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The map label for this gene is ruvA

Identifier: 161936257

GI number: 161936257

Start: 499287

End: 499952

Strand: Direct

Name: ruvA

Synonym: mhp421

Alternate gene names: 161936257

Gene position: 499287-499952 (Clockwise)

Preceding gene: 54020196

Following gene: 54020198

Centisome position: 55.93

GC content: 31.98

Gene sequence:

>666_bases
ATGCAAATCTATCAATTCGGAAAAATTGTCTCAAAAAACAAAAACTATTTAATTCTTGAAAACCACGGCAGTGGCTATCT
TATTTATGTACCTCGAATCGATCGTTTTAGCCGCGACGAAAATCGCAAAATTTATATTTATGAACATGAAAATGACTATA
CAAAAATAACTTACGGTTTTGCGAGCTTCCGTGAGCGGATTTTGTTTGAAGATCTAATTTCAATTCAAGGGGTTGGACCT
AAAACCGCAATTTCTGCACTTAATTCGGGAATGCAGAATTTAATTAATTTAATTGCCGCGAACGACTGAAAAACGCTGGC
AAAAATTCCTTATCTTTCTGAGAAAAATGCAAAACAAATAGTCTTTGAATTTCAAAAAAAATACGAAAGATTTAACGAAA
ACCATAAAAACCAAACAGAGGAAACAAATCCAGAGACTCAAGAAAAAGAACTAGAAAAAGAGGATGATTTAGCTGATATT
ACCATCCAAAAATCAAATCTTGAAGACAAAACAGCCGCAAATCTTGAGGATACTTTGAAAATGCTTGGTTTCAAACCACG
GCAAATTGATTATGCGCTTACAAAAGTTGAACCTAATGAAAATTTTGAAAATTTAATTGAAAACGCAATTAAAATTATAT
CAAATGCCAGAGAATTTAGAAATTAG

Upstream 100 bases:

>100_bases
GGAAAATTAACCAATTTTTCCCTATTTTTTCCTTTTTTAAAGTTTTTTTGCAAAAAATTTTAATTTTAGTTGAAACAAAA
CAACCAAAAGGGAGCAAAAA

Downstream 100 bases:

>100_bases
ACCAAGCAGTTTTGAAAATTTTATCGGGCAAAAAAAATTAGTTGAAACCTTACAAATTTTAATTAGCTCATCACAAAAAC
GGAAGCAATCACTTGATCAT

Product: Holliday junction DNA helicase RuvA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 221; Mature: 221

Protein sequence:

>221_residues
MQIYQFGKIVSKNKNYLILENHGSGYLIYVPRIDRFSRDENRKIYIYEHENDYTKITYGFASFRERILFEDLISIQGVGP
KTAISALNSGMQNLINLIAANDWKTLAKIPYLSEKNAKQIVFEFQKKYERFNENHKNQTEETNPETQEKELEKEDDLADI
TIQKSNLEDKTAANLEDTLKMLGFKPRQIDYALTKVEPNENFENLIENAIKIISNAREFRN

Sequences:

>Translated_221_residues
MQIYQFGKIVSKNKNYLILENHGSGYLIYVPRIDRFSRDENRKIYIYEHENDYTKITYGFASFRERILFEDLISIQGVGP
KTAISALNSGMQNLINLIAAND*KTLAKIPYLSEKNAKQIVFEFQKKYERFNENHKNQTEETNPETQEKELEKEDDLADI
TIQKSNLEDKTAANLEDTLKMLGFKPRQIDYALTKVEPNENFENLIENAIKIISNAREFRN
>Mature_221_residues
MQIYQFGKIVSKNKNYLILENHGSGYLIYVPRIDRFSRDENRKIYIYEHENDYTKITYGFASFRERILFEDLISIQGVGP
KTAISALNSGMQNLINLIAAND*KTLAKIPYLSEKNAKQIVFEFQKKYERFNENHKNQTEETNPETQEKELEKEDDLADI
TIQKSNLEDKTAANLEDTLKMLGFKPRQIDYALTKVEPNENFENLIENAIKIISNAREFRN

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG0632

COG function: function code L; Holliday junction resolvasome, DNA-binding subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvA family [H]

Homologues:

Organism=Escherichia coli, GI1788168, Length=211, Percent_Identity=26.5402843601896, Blast_Score=68, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011114
- InterPro:   IPR013849
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000085
- InterPro:   IPR010994 [H]

Pfam domain/function: PF07499 RuvA_C; PF01330 RuvA_N [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 25710; Mature: 25710

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIYQFGKIVSKNKNYLILENHGSGYLIYVPRIDRFSRDENRKIYIYEHENDYTKITYGF
CCHHHHHHHHHCCCCEEEEEECCCEEEEEECCCCCCCCCCCCEEEEEECCCCCEEEEECH
ASFRERILFEDLISIQGVGPKTAISALNSGMQNLINLIAANDKTLAKIPYLSEKNAKQIV
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHH
FEFQKKYERFNENHKNQTEETNPETQEKELEKEDDLADITIQKSNLEDKTAANLEDTLKM
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCHHHCCHHHHHHH
LGFKPRQIDYALTKVEPNENFENLIENAIKIISNAREFRN
HCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQIYQFGKIVSKNKNYLILENHGSGYLIYVPRIDRFSRDENRKIYIYEHENDYTKITYGF
CCHHHHHHHHHCCCCEEEEEECCCEEEEEECCCCCCCCCCCCEEEEEECCCCCEEEEECH
ASFRERILFEDLISIQGVGPKTAISALNSGMQNLINLIAANDKTLAKIPYLSEKNAKQIV
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHH
FEFQKKYERFNENHKNQTEETNPETQEKELEKEDDLADITIQKSNLEDKTAANLEDTLKM
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCHHHCCHHHHHHH
LGFKPRQIDYALTKVEPNENFENLIENAIKIISNAREFRN
HCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA