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The map label for this gene is iolD

Identifier: 52787925

GI number: 52787925

Start: 4085602

End: 4087515

Strand: Reverse

Name: iolD

Synonym: BLi04248

Alternate gene names: 52787925

Gene position: 4087515-4085602 (Counterclockwise)

Preceding gene: 52787926

Following gene: 52787924

Centisome position: 96.8

GC content: 47.81

Gene sequence:

>1914_bases
GTGGGCAAGAAAATACGTTTAACCACAGCACAAGCGTTAATTAAGTTCTTAAATCAGCAGTACATCCATGTAGATGGAGA
AGAAACTCCGTTTGTCGAGGGAATCTTTACGATTTTTGGCCATGGGAACGTCGTTGGAATCGGCCAGGCGCTTGAACAGG
ATGCCGGCCACTTAAAGGTGTTTCAAGGCAAAAATGAGCAGGGAATGGCGCATGCAGCAATGGCTTACAGCAAGCAAATG
CTGAGAAGGAAGATCTATGCCGTGTCGACATCAGTCGGACCGGGAGCAGCTAACCTTGTGGCAGCCGCAGGTACTGCGCT
TGCGAACAACATTCCAGTCCTCTTGATTCCAGCCGATACATTTGCCACGAGACAGCCTGATCCTGTTCTTCAGCAAGTAG
AGCAGGAATACAGTGCGGCGATTACGACGAACGATGCATTGAAGCCTGTTTCGAGGTATTGGGACCGTATTACGCGCCCT
GAACAGTTGATGAGCAGTCTGATTCGCGCTTTTGAAGTAATGACAGACCCTGCAAAAGCTGGTCCTGCAACGATTTGCAT
TTCTCAAGATGTTGAAGGCGAGGCCTATGATTTCGATGAAAGCTTTTTTGAAAAACGCGTTCACTATGTTGATCGCAAAG
AGCCGAGCGAGCGTGAACTGAAGGGAGCGGCAGAACTGATCAAGTCGAGCAAAAAACCGCTCATCTTGGTCGGCGGCGGA
GCGAAATATTCCGGCGCGCGCGACGAATTGATCGCCATGTCTGAAGCTTACAATATTCCGCTGGTAGAAACCCAGGCCGG
TAAATCCACAGTAGAAGCCGATTTTGCCAACAATTTGGGCGGAATGGGAATCACCGGTACGCTGGCGGCAAACAAAGCGG
CTCGCGGGGCAGACCTGATCATCGGAATTGGCACGCGCTATACCGATTTTGCGACATCATCAAAAACCGCTTTTGACTTT
GACAACGCAAAATTTTTAAACATTAATGTCAGCAGAATGCAGGCTTATAAACTGGATGCTTTCCAAGTCGTTGCCGATGC
AAAAGTAACGCTCGGCAAACTGCACGGCTTATTGGAAGGCTATAAGAGCGAGTTCGGTTCAACCATCAAGGAGCTGAAAG
ACGAATGGCTTGCTGAACGCGACCGCTTAAGCAAGGTAACATTTAAGCGCGAAAACTTCACTCCTGAAATTAAAGATCAC
TTTTCTCAAGACATACTCAACGAATATGCGGATGTGCTGCAAACCGAACTGCCGCAAACAACGGCATTGCTCGCCATCAA
TGAAACAGTTGATCCGGACAGCGTCGTCATTTGTTCAGCAGGCTCTTTGCCGGGAGATCTGCAGCGGCTGTGGCATTCAA
ACGTACCGAATACGTACCACCTGGAATACGGCTATTCCTGCATGGGATATGAAGTATCGGGAACGCTCGGCTTGAAATTG
GCGCATCCTGATAGAGAAGTTTATTCACTGGTCGGAGACGGAAGCTTCCTGATGCTCCACTCAGAGCTCATTACAGCAAT
CCAATATAATAAAAAAATTAACGTGCTGCTCTTTGACAACTCAGGATTCGGCTGCATCAACAATCTGCAAATGGACCATG
GAAACGGCAGCTACTACTGTGAATTCAGAACAGCAGACAATCAGATTCTCAATGTCGATTATGCGAAAGTGGCCGAAGGC
TATGGAGCGAAAACCTACAAAGCGAACACGATTGAAGAATTAAAAGCGGCGCTGGAAGATGCCAAAAAACAAGATGTCTC
AACATTGATTGAAATGAAGGTGCTGCCGAAAACGATGACGGACGGCTATGACAGCTGGTGGCACGTAGGTGTTGCAGAGG
TTTCCGAACAGGAAAGCGTGCAAAAGGCGTATAAAGCAAAAGAAAAAATGCTGGAATCTGCAAAGCAGTATTAA

Upstream 100 bases:

>100_bases
AAGCACAGCTCATCTGAAGCGATGCCGACAGTCGCCGAGATTGAAGAGCTTATTGCGGCTCAGTCTTAAATGAAGAATAT
GAATAAAGGTGGGATCGTTT

Downstream 100 bases:

>100_bases
AGGAGGCACCAGGATGGGCAAGACACAAATTCTATGGGGCATCGCCCCCATAGGATGGCGGAACGATGATATTCCGGAAA
TCGGCGCGGGAAACACACTT

Product: IolD

Products: NA

Alternate protein names: THcHDO hydrolase

Number of amino acids: Translated: 637; Mature: 636

Protein sequence:

>637_residues
MGKKIRLTTAQALIKFLNQQYIHVDGEETPFVEGIFTIFGHGNVVGIGQALEQDAGHLKVFQGKNEQGMAHAAMAYSKQM
LRRKIYAVSTSVGPGAANLVAAAGTALANNIPVLLIPADTFATRQPDPVLQQVEQEYSAAITTNDALKPVSRYWDRITRP
EQLMSSLIRAFEVMTDPAKAGPATICISQDVEGEAYDFDESFFEKRVHYVDRKEPSERELKGAAELIKSSKKPLILVGGG
AKYSGARDELIAMSEAYNIPLVETQAGKSTVEADFANNLGGMGITGTLAANKAARGADLIIGIGTRYTDFATSSKTAFDF
DNAKFLNINVSRMQAYKLDAFQVVADAKVTLGKLHGLLEGYKSEFGSTIKELKDEWLAERDRLSKVTFKRENFTPEIKDH
FSQDILNEYADVLQTELPQTTALLAINETVDPDSVVICSAGSLPGDLQRLWHSNVPNTYHLEYGYSCMGYEVSGTLGLKL
AHPDREVYSLVGDGSFLMLHSELITAIQYNKKINVLLFDNSGFGCINNLQMDHGNGSYYCEFRTADNQILNVDYAKVAEG
YGAKTYKANTIEELKAALEDAKKQDVSTLIEMKVLPKTMTDGYDSWWHVGVAEVSEQESVQKAYKAKEKMLESAKQY

Sequences:

>Translated_637_residues
MGKKIRLTTAQALIKFLNQQYIHVDGEETPFVEGIFTIFGHGNVVGIGQALEQDAGHLKVFQGKNEQGMAHAAMAYSKQM
LRRKIYAVSTSVGPGAANLVAAAGTALANNIPVLLIPADTFATRQPDPVLQQVEQEYSAAITTNDALKPVSRYWDRITRP
EQLMSSLIRAFEVMTDPAKAGPATICISQDVEGEAYDFDESFFEKRVHYVDRKEPSERELKGAAELIKSSKKPLILVGGG
AKYSGARDELIAMSEAYNIPLVETQAGKSTVEADFANNLGGMGITGTLAANKAARGADLIIGIGTRYTDFATSSKTAFDF
DNAKFLNINVSRMQAYKLDAFQVVADAKVTLGKLHGLLEGYKSEFGSTIKELKDEWLAERDRLSKVTFKRENFTPEIKDH
FSQDILNEYADVLQTELPQTTALLAINETVDPDSVVICSAGSLPGDLQRLWHSNVPNTYHLEYGYSCMGYEVSGTLGLKL
AHPDREVYSLVGDGSFLMLHSELITAIQYNKKINVLLFDNSGFGCINNLQMDHGNGSYYCEFRTADNQILNVDYAKVAEG
YGAKTYKANTIEELKAALEDAKKQDVSTLIEMKVLPKTMTDGYDSWWHVGVAEVSEQESVQKAYKAKEKMLESAKQY
>Mature_636_residues
GKKIRLTTAQALIKFLNQQYIHVDGEETPFVEGIFTIFGHGNVVGIGQALEQDAGHLKVFQGKNEQGMAHAAMAYSKQML
RRKIYAVSTSVGPGAANLVAAAGTALANNIPVLLIPADTFATRQPDPVLQQVEQEYSAAITTNDALKPVSRYWDRITRPE
QLMSSLIRAFEVMTDPAKAGPATICISQDVEGEAYDFDESFFEKRVHYVDRKEPSERELKGAAELIKSSKKPLILVGGGA
KYSGARDELIAMSEAYNIPLVETQAGKSTVEADFANNLGGMGITGTLAANKAARGADLIIGIGTRYTDFATSSKTAFDFD
NAKFLNINVSRMQAYKLDAFQVVADAKVTLGKLHGLLEGYKSEFGSTIKELKDEWLAERDRLSKVTFKRENFTPEIKDHF
SQDILNEYADVLQTELPQTTALLAINETVDPDSVVICSAGSLPGDLQRLWHSNVPNTYHLEYGYSCMGYEVSGTLGLKLA
HPDREVYSLVGDGSFLMLHSELITAIQYNKKINVLLFDNSGFGCINNLQMDHGNGSYYCEFRTADNQILNVDYAKVAEGY
GAKTYKANTIEELKAALEDAKKQDVSTLIEMKVLPKTMTDGYDSWWHVGVAEVSEQESVQKAYKAKEKMLESAKQY

Specific function: Involved in the cleavage of the C1-C2 bond of 3D- (3,5/4)-trihydroxycyclohexane-1,2-dione (THcHDO) to yield 5-deoxy- glucuronate (5DG)

COG id: COG3962

COG function: function code E; Acetolactate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family

Homologues:

Organism=Escherichia coli, GI1790104, Length=597, Percent_Identity=22.9480737018425, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI87081685, Length=562, Percent_Identity=24.5551601423488, Blast_Score=129, Evalue=7e-31,
Organism=Escherichia coli, GI1786717, Length=563, Percent_Identity=22.5577264653641, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI1787096, Length=524, Percent_Identity=21.9465648854962, Blast_Score=99, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6323755, Length=594, Percent_Identity=23.7373737373737, Blast_Score=127, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): IOLD_BACLD (Q65D03)

Other databases:

- EMBL:   CP000002
- EMBL:   AE017333
- RefSeq:   YP_081320.1
- RefSeq:   YP_093754.1
- HSSP:   P37063
- ProteinModelPortal:   Q65D03
- SMR:   Q65D03
- STRING:   Q65D03
- EnsemblBacteria:   EBBACT00000054817
- EnsemblBacteria:   EBBACT00000061493
- GeneID:   3030847
- GeneID:   3098106
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi04248
- KEGG:   bli:BL00243
- NMPDR:   fig|279010.5.peg.4027
- eggNOG:   COG3962
- GeneTree:   EBGT00070000032034
- HOGENOM:   HBG294332
- OMA:   FAKQHRR
- ProtClustDB:   CLSK2300076
- BioCyc:   BLIC279010-1:BLI04248-MONOMER
- BioCyc:   BLIC279010:BL00243-MONOMER
- HAMAP:   MF_01669
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N

EC number: 1.-.-.-

Molecular weight: Translated: 70180; Mature: 70049

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00187 TPP_ENZYMES

Important sites: BINDING 66-66

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKKIRLTTAQALIKFLNQQYIHVDGEETPFVEGIFTIFGHGNVVGIGQALEQDAGHLKV
CCCEEEEHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHCCCCCEEECCHHHHCCCCCEEE
FQGKNEQGMAHAAMAYSKQMLRRKIYAVSTSVGPGAANLVAAAGTALANNIPVLLIPADT
EECCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHCCCCEEEECCCC
FATRQPDPVLQQVEQEYSAAITTNDALKPVSRYWDRITRPEQLMSSLIRAFEVMTDPAKA
CCCCCCCHHHHHHHHHHHEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
GPATICISQDVEGEAYDFDESFFEKRVHYVDRKEPSERELKGAAELIKSSKKPLILVGGG
CCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEECC
AKYSGARDELIAMSEAYNIPLVETQAGKSTVEADFANNLGGMGITGTLAANKAARGADLI
CCCCCCCHHHEEHHHHCCCCEEEECCCCCCCHHHHHHCCCCCCEEEEECCCCCCCCCEEE
IGIGTRYTDFATSSKTAFDFDNAKFLNINVSRMQAYKLDAFQVVADAKVTLGKLHGLLEG
EECCCCCCCCCCCCCCEEECCCEEEEEEEHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHH
YKSEFGSTIKELKDEWLAERDRLSKVTFKRENFTPEIKDHFSQDILNEYADVLQTELPQT
HHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
TALLAINETVDPDSVVICSAGSLPGDLQRLWHSNVPNTYHLEYGYSCMGYEVSGTLGLKL
EEEEEEECCCCCCCEEEEECCCCCHHHHHHHHCCCCCEEEEECCCEEEEEEECCEEEEEE
AHPDREVYSLVGDGSFLMLHSELITAIQYNKKINVLLFDNSGFGCINNLQMDHGNGSYYC
ECCCHHHHHHHCCCCEEEEHHHHHHHHHCCCEEEEEEECCCCCCEECCCEEECCCCCEEE
EFRTADNQILNVDYAKVAEGYGAKTYKANTIEELKAALEDAKKQDVSTLIEMKVLPKTMT
EEECCCCEEEECCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHC
DGYDSWWHVGVAEVSEQESVQKAYKAKEKMLESAKQY
CCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GKKIRLTTAQALIKFLNQQYIHVDGEETPFVEGIFTIFGHGNVVGIGQALEQDAGHLKV
CCEEEEHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHCCCCCEEECCHHHHCCCCCEEE
FQGKNEQGMAHAAMAYSKQMLRRKIYAVSTSVGPGAANLVAAAGTALANNIPVLLIPADT
EECCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHCCCCEEEECCCC
FATRQPDPVLQQVEQEYSAAITTNDALKPVSRYWDRITRPEQLMSSLIRAFEVMTDPAKA
CCCCCCCHHHHHHHHHHHEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
GPATICISQDVEGEAYDFDESFFEKRVHYVDRKEPSERELKGAAELIKSSKKPLILVGGG
CCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEECC
AKYSGARDELIAMSEAYNIPLVETQAGKSTVEADFANNLGGMGITGTLAANKAARGADLI
CCCCCCCHHHEEHHHHCCCCEEEECCCCCCCHHHHHHCCCCCCEEEEECCCCCCCCCEEE
IGIGTRYTDFATSSKTAFDFDNAKFLNINVSRMQAYKLDAFQVVADAKVTLGKLHGLLEG
EECCCCCCCCCCCCCCEEECCCEEEEEEEHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHH
YKSEFGSTIKELKDEWLAERDRLSKVTFKRENFTPEIKDHFSQDILNEYADVLQTELPQT
HHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
TALLAINETVDPDSVVICSAGSLPGDLQRLWHSNVPNTYHLEYGYSCMGYEVSGTLGLKL
EEEEEEECCCCCCCEEEEECCCCCHHHHHHHHCCCCCEEEEECCCEEEEEEECCEEEEEE
AHPDREVYSLVGDGSFLMLHSELITAIQYNKKINVLLFDNSGFGCINNLQMDHGNGSYYC
ECCCHHHHHHHCCCCEEEEHHHHHHHHHCCCEEEEEEECCCCCCEECCCEEECCCCCEEE
EFRTADNQILNVDYAKVAEGYGAKTYKANTIEELKAALEDAKKQDVSTLIEMKVLPKTMT
EEECCCCEEEECCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHC
DGYDSWWHVGVAEVSEQESVQKAYKAKEKMLESAKQY
CCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA