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The map label for this gene is iolE

Identifier: 52787924

GI number: 52787924

Start: 4084686

End: 4085588

Strand: Reverse

Name: iolE

Synonym: BLi04247

Alternate gene names: 52787924

Gene position: 4085588-4084686 (Counterclockwise)

Preceding gene: 52787925

Following gene: 52787923

Centisome position: 96.75

GC content: 49.61

Gene sequence:

>903_bases
ATGGGCAAGACACAAATTCTATGGGGCATCGCCCCCATAGGATGGCGGAACGATGATATTCCGGAAATCGGCGCGGGAAA
CACACTTCAGCATTTGTTGAGCGACATTGTTGTTGCGGGCTTTCAAGGCACGGAAGTGGGCGGCTTTTTTCCGGAACCGG
CCATTCTCAATAAAGAGCTCGAGCTGAGGAATCTAAGGATTGCCGGCAAGTGGTTCAGCAGCTATATCATTCGGGACGGC
ATTGAAGAAGCAGCCAAGGAATTTGCGGCTCACTGCCAGTACTTAAAAGACGTCCATGCCGATGTTGCGGTCGTATCAGA
ACAGACGTACAGTGTGCAGGGCTTGGATAAAAACGTGTTTAAAGAAAAGCCTTATTTCACCGATGAAGAATGGCAGCGGC
TATTTGAAGGTTTAAATCACCTCGGGGAAATCGCCGGCCGTTACGGACTGAAGCTTGTCTACCATCACCACCTCGGCACA
GGCGTGCAGACAGAGGAAGAGGTGGACCGGCTCATGGCGGGAACTGACCCGGCACTTGTGCACCTTCTGTACGATACGGG
GCATGCCTACATTTCAGATGGAAATTATATGAACATTTTAGAGAAGCATATTGACCGTATTAGGCACGTTCATTTTAAAG
ACGCGCGCCTTAAGATCATGGAGAAATGCAAGCGGGAAGGAAATTCGTTCCAACAGGCGTTTCTGCAAGGGATGTTCACA
GTTCCAGGCGACGGCTGCATCGATTTTAGAGAAGTATATCAAACGCTTTTAAAGCACGGCTATTCCGGCTGGATCGTAGT
GGAAGCCGAGCAGGATCCCGATGTTGCCAACCCGCTTGAATACGCATTGATCGCCAGAAAGTACATCGATCGTCACTTGT
TGAATGTCCCAGCAACCAATTAA

Upstream 100 bases:

>100_bases
GGTGTTGCAGAGGTTTCCGAACAGGAAAGCGTGCAAAAGGCGTATAAAGCAAAAGAAAAAATGCTGGAATCTGCAAAGCA
GTATTAAAGGAGGCACCAGG

Downstream 100 bases:

>100_bases
GGGGGCTCTAAATTGAGCAATGGAAGTGCAGCAGTCTCACCATTCAATAAACGAACCGTCGCAGCAGCTTTGGCAAATTA
CATTGATGCAGGCTCCATTG

Product: hypothetical protein

Products: NA

Alternate protein names: 2-keto-myo-inositol dehydratase; 2KMI dehydratase

Number of amino acids: Translated: 300; Mature: 299

Protein sequence:

>300_residues
MGKTQILWGIAPIGWRNDDIPEIGAGNTLQHLLSDIVVAGFQGTEVGGFFPEPAILNKELELRNLRIAGKWFSSYIIRDG
IEEAAKEFAAHCQYLKDVHADVAVVSEQTYSVQGLDKNVFKEKPYFTDEEWQRLFEGLNHLGEIAGRYGLKLVYHHHLGT
GVQTEEEVDRLMAGTDPALVHLLYDTGHAYISDGNYMNILEKHIDRIRHVHFKDARLKIMEKCKREGNSFQQAFLQGMFT
VPGDGCIDFREVYQTLLKHGYSGWIVVEAEQDPDVANPLEYALIARKYIDRHLLNVPATN

Sequences:

>Translated_300_residues
MGKTQILWGIAPIGWRNDDIPEIGAGNTLQHLLSDIVVAGFQGTEVGGFFPEPAILNKELELRNLRIAGKWFSSYIIRDG
IEEAAKEFAAHCQYLKDVHADVAVVSEQTYSVQGLDKNVFKEKPYFTDEEWQRLFEGLNHLGEIAGRYGLKLVYHHHLGT
GVQTEEEVDRLMAGTDPALVHLLYDTGHAYISDGNYMNILEKHIDRIRHVHFKDARLKIMEKCKREGNSFQQAFLQGMFT
VPGDGCIDFREVYQTLLKHGYSGWIVVEAEQDPDVANPLEYALIARKYIDRHLLNVPATN
>Mature_299_residues
GKTQILWGIAPIGWRNDDIPEIGAGNTLQHLLSDIVVAGFQGTEVGGFFPEPAILNKELELRNLRIAGKWFSSYIIRDGI
EEAAKEFAAHCQYLKDVHADVAVVSEQTYSVQGLDKNVFKEKPYFTDEEWQRLFEGLNHLGEIAGRYGLKLVYHHHLGTG
VQTEEEVDRLMAGTDPALVHLLYDTGHAYISDGNYMNILEKHIDRIRHVHFKDARLKIMEKCKREGNSFQQAFLQGMFTV
PGDGCIDFREVYQTLLKHGYSGWIVVEAEQDPDVANPLEYALIARKYIDRHLLNVPATN

Specific function: Catalyzes the dehydration of inosose (2-keto-myo- inositol, 2KMI or 2,4,6/3,5-pentahydroxycyclohexanone) to 3D- (3,5/4)-trihydroxycyclohexane-1,2-dione (D-2,3-diketo-4-deoxy-epi- inositol)

COG id: COG1082

COG function: function code G; Sugar phosphate isomerases/epimerases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iolE/mocC family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): IOLE_BACLD (Q65D04)

Other databases:

- EMBL:   CP000002
- EMBL:   AE017333
- RefSeq:   YP_081319.1
- RefSeq:   YP_093753.1
- ProteinModelPortal:   Q65D04
- SMR:   Q65D04
- STRING:   Q65D04
- EnsemblBacteria:   EBBACT00000055497
- EnsemblBacteria:   EBBACT00000061104
- GeneID:   3030851
- GeneID:   3099808
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi04247
- KEGG:   bli:BL00242
- NMPDR:   fig|279010.5.peg.4026
- eggNOG:   COG1082
- GeneTree:   EBGT00050000002447
- HOGENOM:   HBG654552
- OMA:   DLGKENT
- ProtClustDB:   CLSK873288
- BioCyc:   BLIC279010-1:BLI04247-MONOMER
- BioCyc:   BLIC279010:BL00242-MONOMER
- HAMAP:   MF_01672
- InterPro:   IPR013022
- InterPro:   IPR012307
- Gene3D:   G3DSA:3.20.20.150

Pfam domain/function: PF01261 AP_endonuc_2; SSF51658 Xyl_isomerase-like_TIM-brl

EC number: =4.2.1.44

Molecular weight: Translated: 34053; Mature: 33922

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKTQILWGIAPIGWRNDDIPEIGAGNTLQHLLSDIVVAGFQGTEVGGFFPEPAILNKEL
CCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCH
ELRNLRIAGKWFSSYIIRDGIEEAAKEFAAHCQYLKDVHADVAVVSEQTYSVQGLDKNVF
HHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHH
KEKPYFTDEEWQRLFEGLNHLGEIAGRYGLKLVYHHHLGTGVQTEEEVDRLMAGTDPALV
HCCCCCCCHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCHHHHHHHHCCCCHHHE
HLLYDTGHAYISDGNYMNILEKHIDRIRHVHFKDARLKIMEKCKREGNSFQQAFLQGMFT
EEEEECCCEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHCCCC
VPGDGCIDFREVYQTLLKHGYSGWIVVEAEQDPDVANPLEYALIARKYIDRHLLNVPATN
CCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
GKTQILWGIAPIGWRNDDIPEIGAGNTLQHLLSDIVVAGFQGTEVGGFFPEPAILNKEL
CCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCH
ELRNLRIAGKWFSSYIIRDGIEEAAKEFAAHCQYLKDVHADVAVVSEQTYSVQGLDKNVF
HHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHH
KEKPYFTDEEWQRLFEGLNHLGEIAGRYGLKLVYHHHLGTGVQTEEEVDRLMAGTDPALV
HCCCCCCCHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCHHHHHHHHCCCCHHHE
HLLYDTGHAYISDGNYMNILEKHIDRIRHVHFKDARLKIMEKCKREGNSFQQAFLQGMFT
EEEEECCCEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHCCCC
VPGDGCIDFREVYQTLLKHGYSGWIVVEAEQDPDVANPLEYALIARKYIDRHLLNVPATN
CCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA