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The map label for this gene is mls [H]

Identifier: 52787885

GI number: 52787885

Start: 4038733

End: 4040322

Strand: Reverse

Name: mls [H]

Synonym: BLi04208

Alternate gene names: 52787885

Gene position: 4040322-4038733 (Counterclockwise)

Preceding gene: 52787887

Following gene: 52787884

Centisome position: 95.68

GC content: 49.31

Gene sequence:

>1590_bases
ATGAAAACAGAGGAGAAGAGAATTCATATAACCGGATCGATAAATCCGCATTATGAAGAGATTTTGACAAAAGAAGCGAT
GGAGTTTATCGGACGTCTTGAACGCCATTTCGGGGGAAGAAGACGGGAATTGCTGCATTTGCGGACGATCAGGCAGGCTG
AAATTGATCAAGGGAAGAACCCTTGTTTTTTGGCGGAAACGAAGCACATCCGCGAGGCGGACTGGACAGTCGCGCCGCTC
CCCCGGGATTTGGAGGACCGCAGGGTTGAAATCACTGGCCCGGTCGACAGAAAAATGGTCATCAACGCTTTAAATTCAGG
AGCGAAAGTCTTTATGGCCGACTTTGAAGATGCGAACTCGCCGACGTGGAAAAACTGCATTGAAGGCCAGGTTAATTTGC
GGGATGCGGTCCGCGGGACGATTTCTTTTACGAATGAACAGGGCAAACGCTATGAGCTGAAAGAGAACCCCGCCGTTATC
ATGGTCAGGCCGAGGGGATGGCACTTGGAAGAAAAGCATGTGCTGATCGGCGGCAGGCCCGTGTCAGCAAGCCTGTTCGA
TTTTGGTCTTTATTTTTTCCATAACGCAAAAGAGCTGATCAAAAAAGGAAGCGGCCCTTATTTCTACCTTCCAAAAATCG
AAAACCACCTGGAAGCAAGGCTGTGGAACGACGTATTTGTTTATGCGCAAAACGACTTGCGCATTCCGCAGGGCACGATT
AAAGCGACGGTTCTCGTTGAAACGATCCTGGCGGCCTTTGAAATGGATGAAATCCTCTACGAATTGAAGGAACATTCAGC
CGGGCTTAACTGCGGCAGGTGGGATTATATTTTCAGCTTTATTAAGAAATTCAGGAACAGGGATGAAGTCATTCTTCCAG
ATCGCTCAGCCGTGACGATGACAGTTCCCAACATGAGAGCCTACTCCCTCTTGGCGATTCAAACGTGTCATAAACGCAAC
GTCCATGCGATTGGGGGAATGGCGGCGCAAATTCCCGCTAAAGACAAACCTGAGATCAATGAAGCCGCATTTGCCAAAAT
CCGCGAAGATAAGGAACGTGAAGTAAAAGACGGCCATGACGGAACATGGATCGCGCATCCCGGCATGATTCAAACGGCGC
TCGATGTATTCAATCAGCATATGCCGTCGGCGAACCAGATTCATAGAAAGCGGAAAGACGTAAAGGTCGAGGAAAAAGAC
TTGCTGGAAGTACCGAAAGGCAGAATTACAGAAGCAGGCGTCCGTACCAATATCAGCGCCGGCATCCGCTATTTAGCTTC
ATGGCTGTCGGGCAGAGGCGCCGCTCCGATCGACCACTTAATGGAGGACGCTGCAACGGCGGAGATTTCCAGAGCCCAGC
TGTGGCAATGGATTCGCCATCCGAAAGGAGTTCTTGACGATGGCCGGAAAGTCACCGCGGAAATGGTCAAGATGATGAAA
GAGAAAGAAATGCAGAAAATCATCGATGAAGCAGGCGTGGAACATTTTCGGAGCGGCCGGTTTGAAGAGGCCGGACAGTT
ATTTGAACAGCTGATTTTGGATGATGAATTCGCGGAGTTTCTAACGATACCGGCTTATGAAAATATATAA

Upstream 100 bases:

>100_bases
GCAGGCTGAATCTTACTTCATGGAATGCGAGCGCTGTCTTGCCAAAAAAGGCGAGTAGGCAAACTGTTTTATAACAAAGC
ATGTAAAGGGGTAGGGAGAC

Downstream 100 bases:

>100_bases
TGGGGGAATGTGCGATGCTGAAAGAAAAACAACTCGCAGAAAGCTGGGAGCATGATGAACGCTGGAAAGGGATCACAAGG
CCGTATGAGGCGGCAGACGT

Product: malate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 529; Mature: 529

Protein sequence:

>529_residues
MKTEEKRIHITGSINPHYEEILTKEAMEFIGRLERHFGGRRRELLHLRTIRQAEIDQGKNPCFLAETKHIREADWTVAPL
PRDLEDRRVEITGPVDRKMVINALNSGAKVFMADFEDANSPTWKNCIEGQVNLRDAVRGTISFTNEQGKRYELKENPAVI
MVRPRGWHLEEKHVLIGGRPVSASLFDFGLYFFHNAKELIKKGSGPYFYLPKIENHLEARLWNDVFVYAQNDLRIPQGTI
KATVLVETILAAFEMDEILYELKEHSAGLNCGRWDYIFSFIKKFRNRDEVILPDRSAVTMTVPNMRAYSLLAIQTCHKRN
VHAIGGMAAQIPAKDKPEINEAAFAKIREDKEREVKDGHDGTWIAHPGMIQTALDVFNQHMPSANQIHRKRKDVKVEEKD
LLEVPKGRITEAGVRTNISAGIRYLASWLSGRGAAPIDHLMEDAATAEISRAQLWQWIRHPKGVLDDGRKVTAEMVKMMK
EKEMQKIIDEAGVEHFRSGRFEEAGQLFEQLILDDEFAEFLTIPAYENI

Sequences:

>Translated_529_residues
MKTEEKRIHITGSINPHYEEILTKEAMEFIGRLERHFGGRRRELLHLRTIRQAEIDQGKNPCFLAETKHIREADWTVAPL
PRDLEDRRVEITGPVDRKMVINALNSGAKVFMADFEDANSPTWKNCIEGQVNLRDAVRGTISFTNEQGKRYELKENPAVI
MVRPRGWHLEEKHVLIGGRPVSASLFDFGLYFFHNAKELIKKGSGPYFYLPKIENHLEARLWNDVFVYAQNDLRIPQGTI
KATVLVETILAAFEMDEILYELKEHSAGLNCGRWDYIFSFIKKFRNRDEVILPDRSAVTMTVPNMRAYSLLAIQTCHKRN
VHAIGGMAAQIPAKDKPEINEAAFAKIREDKEREVKDGHDGTWIAHPGMIQTALDVFNQHMPSANQIHRKRKDVKVEEKD
LLEVPKGRITEAGVRTNISAGIRYLASWLSGRGAAPIDHLMEDAATAEISRAQLWQWIRHPKGVLDDGRKVTAEMVKMMK
EKEMQKIIDEAGVEHFRSGRFEEAGQLFEQLILDDEFAEFLTIPAYENI
>Mature_529_residues
MKTEEKRIHITGSINPHYEEILTKEAMEFIGRLERHFGGRRRELLHLRTIRQAEIDQGKNPCFLAETKHIREADWTVAPL
PRDLEDRRVEITGPVDRKMVINALNSGAKVFMADFEDANSPTWKNCIEGQVNLRDAVRGTISFTNEQGKRYELKENPAVI
MVRPRGWHLEEKHVLIGGRPVSASLFDFGLYFFHNAKELIKKGSGPYFYLPKIENHLEARLWNDVFVYAQNDLRIPQGTI
KATVLVETILAAFEMDEILYELKEHSAGLNCGRWDYIFSFIKKFRNRDEVILPDRSAVTMTVPNMRAYSLLAIQTCHKRN
VHAIGGMAAQIPAKDKPEINEAAFAKIREDKEREVKDGHDGTWIAHPGMIQTALDVFNQHMPSANQIHRKRKDVKVEEKD
LLEVPKGRITEAGVRTNISAGIRYLASWLSGRGAAPIDHLMEDAATAEISRAQLWQWIRHPKGVLDDGRKVTAEMVKMMK
EKEMQKIIDEAGVEHFRSGRFEEAGQLFEQLILDDEFAEFLTIPAYENI

Specific function: Glyoxylate bypass; second step. [C]

COG id: COG2225

COG function: function code C; Malate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1790444, Length=510, Percent_Identity=54.1176470588235, Blast_Score=564, Evalue=1e-162,
Organism=Caenorhabditis elegans, GI17561814, Length=514, Percent_Identity=53.6964980544747, Blast_Score=552, Evalue=1e-157,
Organism=Caenorhabditis elegans, GI71982926, Length=407, Percent_Identity=56.2653562653563, Blast_Score=467, Evalue=1e-132,
Organism=Saccharomyces cerevisiae, GI6324212, Length=517, Percent_Identity=50.2901353965184, Blast_Score=515, Evalue=1e-147,
Organism=Saccharomyces cerevisiae, GI6322222, Length=517, Percent_Identity=49.9032882011605, Blast_Score=514, Evalue=1e-147,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011076
- InterPro:   IPR006252
- InterPro:   IPR001465
- InterPro:   IPR019830 [H]

Pfam domain/function: PF01274 Malate_synthase [H]

EC number: =2.3.3.9 [H]

Molecular weight: Translated: 60642; Mature: 60642

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS00510 MALATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTEEKRIHITGSINPHYEEILTKEAMEFIGRLERHFGGRRRELLHLRTIRQAEIDQGKN
CCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCC
PCFLAETKHIREADWTVAPLPRDLEDRRVEITGPVDRKMVINALNSGAKVFMADFEDANS
CEEEECCCHHCCCCCEECCCCCCCCCCEEEEECCCCHHHHHHHHCCCCEEEEEECCCCCC
PTWKNCIEGQVNLRDAVRGTISFTNEQGKRYELKENPAVIMVRPRGWHLEEKHVLIGGRP
CCHHHHHCCCCCHHHHHCCCEEEECCCCCEEEECCCCEEEEECCCCCCCCCCEEEECCCC
VSASLFDFGLYFFHNAKELIKKGSGPYFYLPKIENHLEARLWNDVFVYAQNDLRIPQGTI
CCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHCCCEEEEEECCCCCCCCHH
KATVLVETILAAFEMDEILYELKEHSAGLNCGRWDYIFSFIKKFRNRDEVILPDRSAVTM
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEECCCCEEEE
TVPNMRAYSLLAIQTCHKRNVHAIGGMAAQIPAKDKPEINEAAFAKIREDKEREVKDGHD
ECCCCCHHHHHHHHHHHCCCCHHHCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
GTWIAHPGMIQTALDVFNQHMPSANQIHRKRKDVKVEEKDLLEVPKGRITEAGVRTNISA
CCEEECCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCCHHH
GIRYLASWLSGRGAAPIDHLMEDAATAEISRAQLWQWIRHPKGVLDDGRKVTAEMVKMMK
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
EKEMQKIIDEAGVEHFRSGRFEEAGQLFEQLILDDEFAEFLTIPAYENI
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHEECCCCCCC
>Mature Secondary Structure
MKTEEKRIHITGSINPHYEEILTKEAMEFIGRLERHFGGRRRELLHLRTIRQAEIDQGKN
CCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCC
PCFLAETKHIREADWTVAPLPRDLEDRRVEITGPVDRKMVINALNSGAKVFMADFEDANS
CEEEECCCHHCCCCCEECCCCCCCCCCEEEEECCCCHHHHHHHHCCCCEEEEEECCCCCC
PTWKNCIEGQVNLRDAVRGTISFTNEQGKRYELKENPAVIMVRPRGWHLEEKHVLIGGRP
CCHHHHHCCCCCHHHHHCCCEEEECCCCCEEEECCCCEEEEECCCCCCCCCCEEEECCCC
VSASLFDFGLYFFHNAKELIKKGSGPYFYLPKIENHLEARLWNDVFVYAQNDLRIPQGTI
CCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHCCCEEEEEECCCCCCCCHH
KATVLVETILAAFEMDEILYELKEHSAGLNCGRWDYIFSFIKKFRNRDEVILPDRSAVTM
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEECCCCEEEE
TVPNMRAYSLLAIQTCHKRNVHAIGGMAAQIPAKDKPEINEAAFAKIREDKEREVKDGHD
ECCCCCHHHHHHHHHHHCCCCHHHCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
GTWIAHPGMIQTALDVFNQHMPSANQIHRKRKDVKVEEKDLLEVPKGRITEAGVRTNISA
CCEEECCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCCHHH
GIRYLASWLSGRGAAPIDHLMEDAATAEISRAQLWQWIRHPKGVLDDGRKVTAEMVKMMK
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
EKEMQKIIDEAGVEHFRSGRFEEAGQLFEQLILDDEFAEFLTIPAYENI
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA