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The map label for this gene is aspA [H]

Identifier: 52787816

GI number: 52787816

Start: 3966432

End: 3967859

Strand: Reverse

Name: aspA [H]

Synonym: BLi04139

Alternate gene names: 52787816

Gene position: 3967859-3966432 (Counterclockwise)

Preceding gene: 52787817

Following gene: 52787815

Centisome position: 93.97

GC content: 49.58

Gene sequence:

>1428_bases
ATGAAAGCTGAAATGACATACCGGACGGAAAAGGATTTTCTCGGAGAGAAGAAGATTCCGGCCGATGTCTACTACGGGAT
TCAGACATTGCGGGCAGCGGAGAACTTCCCGATTACAGGATATAAAATCCACGAAGAAATGATCAAGGCGCTGGCAGTGG
TGAAAAAAGCGGCGGCGCTTGCCAACATGGAGACAAAACGTCTTTATAAAGGCCTTGGAGATGCCATCGTCCAAGCCGCT
GATGAGATCCTTGAAGGAAAGCTGCACGATCAGTTCATCGTTGATCCGATCCAAGGCGGCGCAGGCACTTCAATGAATAT
GAACGCGAACGAAGTCATCGGAAACCGCGCACTTGAACTGCTCGGCCACAACAAAGGCGAATATATTCACCTGAGTCCGA
ATACGCACGTCAACATGTCTCAATCGACAAACGACGTATTCCCGACGGCGATTCATATTTCAACGCTTAAGCTGCTGGAA
AAGCTGCTGGACACAATGGAATACATGCTTGATGCCTTTAAGAAAAAAGCACGCGACTTTGACCACGTCATTAAAATGGG
GCGCACCCATCTTCAAGACGCCGTGCCGATCCGCCTTGGACAGGAGTTTGAAGCCTACAGCCGCGTGATCGAACGCGACA
TCAAACGGATTAAACAATCGCGCCAGCATTTGTATGAAGTCAATATGGGCGCTACCGCGGTTGGAACGGGATTAAACGCG
GATCCGCGCTATATCGAAAATGTCGTCAAGCACTTGGCTGAGATCAGCGGTCTTCCGCTTGTCGGCGCTGACCATCTGGT
AGATGCGACGCAAAATACGGACGCTTACACAGAAGTTTCAGCTGCATTAAAAGTATGCATGATGAACATGTCGAAAATCG
CCAACGACCTTCGGTTGATGGCATCAGGTCCGCGCGCAGGACTTGCGGAGATTTCCCTTCCGGCAAGACAGCCGGGTTCA
TCCATTATGCCGGGGAAAGTCAATCCGGTTATGGCCGAGCTGATCAACCAAATCGCCTTCCAGGTCATCGGAAACGATCA
TACGATCTGCCTTGCTTCTGAAGCCGGACAGCTCGAACTGAATGTGATGGAGCCAGTGCTCGTCTTTAATCTCCTGCAAT
CCATCAGCATCATGAACAACGGATTCCGTTCGTTCACTGATCACTGTGTAGCAGGTATTGAAGCGAATGAAAAACGCTTA
AAAGCATATGTCGAAAAGAGCGCCGGCGTCATCACTGCGGTCAATCCGCATCTTGGCTACGAAGCAGCTGCAAGGATTGC
GAGAGAAGCCATTTTAACAGGCCAATCCGTCCGCGATCTCTGTCTTCAAAACGATGTGTTGACAGAAGAAGAGCTTGATC
TGATTTTGAATCCTTATGAAATGACAAAACCGGGGATTGCCGGGGCTGAACTTTTAAATCGGGATTGA

Upstream 100 bases:

>100_bases
GGGCTTTAGGGAAAAACGGACAACAAGCTGAAGTCAAAAAGATCATGGAAACGCCGATTGCAGACGATATCATCATATAA
AAAGAAAGCAGGGTTAAAAA

Downstream 100 bases:

>100_bases
TCTGGAAAAAACAGTATGCGAAAACACTCTGACATTTTACCGGGATAGAAACACAGAGTGTTTTCGCTTTCCTTCAATAA
CTTTATGAAGAGAAAGGACA

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MKAEMTYRTEKDFLGEKKIPADVYYGIQTLRAAENFPITGYKIHEEMIKALAVVKKAAALANMETKRLYKGLGDAIVQAA
DEILEGKLHDQFIVDPIQGGAGTSMNMNANEVIGNRALELLGHNKGEYIHLSPNTHVNMSQSTNDVFPTAIHISTLKLLE
KLLDTMEYMLDAFKKKARDFDHVIKMGRTHLQDAVPIRLGQEFEAYSRVIERDIKRIKQSRQHLYEVNMGATAVGTGLNA
DPRYIENVVKHLAEISGLPLVGADHLVDATQNTDAYTEVSAALKVCMMNMSKIANDLRLMASGPRAGLAEISLPARQPGS
SIMPGKVNPVMAELINQIAFQVIGNDHTICLASEAGQLELNVMEPVLVFNLLQSISIMNNGFRSFTDHCVAGIEANEKRL
KAYVEKSAGVITAVNPHLGYEAAARIAREAILTGQSVRDLCLQNDVLTEEELDLILNPYEMTKPGIAGAELLNRD

Sequences:

>Translated_475_residues
MKAEMTYRTEKDFLGEKKIPADVYYGIQTLRAAENFPITGYKIHEEMIKALAVVKKAAALANMETKRLYKGLGDAIVQAA
DEILEGKLHDQFIVDPIQGGAGTSMNMNANEVIGNRALELLGHNKGEYIHLSPNTHVNMSQSTNDVFPTAIHISTLKLLE
KLLDTMEYMLDAFKKKARDFDHVIKMGRTHLQDAVPIRLGQEFEAYSRVIERDIKRIKQSRQHLYEVNMGATAVGTGLNA
DPRYIENVVKHLAEISGLPLVGADHLVDATQNTDAYTEVSAALKVCMMNMSKIANDLRLMASGPRAGLAEISLPARQPGS
SIMPGKVNPVMAELINQIAFQVIGNDHTICLASEAGQLELNVMEPVLVFNLLQSISIMNNGFRSFTDHCVAGIEANEKRL
KAYVEKSAGVITAVNPHLGYEAAARIAREAILTGQSVRDLCLQNDVLTEEELDLILNPYEMTKPGIAGAELLNRD
>Mature_475_residues
MKAEMTYRTEKDFLGEKKIPADVYYGIQTLRAAENFPITGYKIHEEMIKALAVVKKAAALANMETKRLYKGLGDAIVQAA
DEILEGKLHDQFIVDPIQGGAGTSMNMNANEVIGNRALELLGHNKGEYIHLSPNTHVNMSQSTNDVFPTAIHISTLKLLE
KLLDTMEYMLDAFKKKARDFDHVIKMGRTHLQDAVPIRLGQEFEAYSRVIERDIKRIKQSRQHLYEVNMGATAVGTGLNA
DPRYIENVVKHLAEISGLPLVGADHLVDATQNTDAYTEVSAALKVCMMNMSKIANDLRLMASGPRAGLAEISLPARQPGS
SIMPGKVNPVMAELINQIAFQVIGNDHTICLASEAGQLELNVMEPVLVFNLLQSISIMNNGFRSFTDHCVAGIEANEKRL
KAYVEKSAGVITAVNPHLGYEAAARIAREAILTGQSVRDLCLQNDVLTEEELDLILNPYEMTKPGIAGAELLNRD

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=467, Percent_Identity=42.8265524625268, Blast_Score=387, Evalue=1e-107,
Organism=Escherichia coli, GI87082375, Length=460, Percent_Identity=49.3478260869565, Blast_Score=441, Evalue=1e-125,
Organism=Escherichia coli, GI1787896, Length=464, Percent_Identity=42.8879310344828, Blast_Score=379, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17553882, Length=460, Percent_Identity=45, Blast_Score=395, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI32565146, Length=327, Percent_Identity=43.1192660550459, Blast_Score=282, Evalue=3e-76,
Organism=Saccharomyces cerevisiae, GI6324993, Length=462, Percent_Identity=43.0735930735931, Blast_Score=387, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24640177, Length=466, Percent_Identity=42.9184549356223, Blast_Score=387, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24640179, Length=466, Percent_Identity=42.9184549356223, Blast_Score=387, Evalue=1e-108,
Organism=Drosophila melanogaster, GI78710009, Length=463, Percent_Identity=42.7645788336933, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24662684, Length=462, Percent_Identity=42.4242424242424, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24583245, Length=462, Percent_Identity=38.3116883116883, Blast_Score=337, Evalue=1e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR005677
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 52278; Mature: 52278

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAEMTYRTEKDFLGEKKIPADVYYGIQTLRAAENFPITGYKIHEEMIKALAVVKKAAAL
CCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
ANMETKRLYKGLGDAIVQAADEILEGKLHDQFIVDPIQGGAGTSMNMNANEVIGNRALEL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHH
LGHNKGEYIHLSPNTHVNMSQSTNDVFPTAIHISTLKLLEKLLDTMEYMLDAFKKKARDF
HCCCCCCEEEECCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DHVIKMGRTHLQDAVPIRLGQEFEAYSRVIERDIKRIKQSRQHLYEVNMGATAVGTGLNA
HHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCC
DPRYIENVVKHLAEISGLPLVGADHLVDATQNTDAYTEVSAALKVCMMNMSKIANDLRLM
CHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ASGPRAGLAEISLPARQPGSSIMPGKVNPVMAELINQIAFQVIGNDHTICLASEAGQLEL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEE
NVMEPVLVFNLLQSISIMNNGFRSFTDHCVAGIEANEKRLKAYVEKSAGVITAVNPHLGY
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEECCCCCH
EAAARIAREAILTGQSVRDLCLQNDVLTEEELDLILNPYEMTKPGIAGAELLNRD
HHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHEECCHHHCCCCCCHHHHHCCC
>Mature Secondary Structure
MKAEMTYRTEKDFLGEKKIPADVYYGIQTLRAAENFPITGYKIHEEMIKALAVVKKAAAL
CCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
ANMETKRLYKGLGDAIVQAADEILEGKLHDQFIVDPIQGGAGTSMNMNANEVIGNRALEL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHH
LGHNKGEYIHLSPNTHVNMSQSTNDVFPTAIHISTLKLLEKLLDTMEYMLDAFKKKARDF
HCCCCCCEEEECCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DHVIKMGRTHLQDAVPIRLGQEFEAYSRVIERDIKRIKQSRQHLYEVNMGATAVGTGLNA
HHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCC
DPRYIENVVKHLAEISGLPLVGADHLVDATQNTDAYTEVSAALKVCMMNMSKIANDLRLM
CHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ASGPRAGLAEISLPARQPGSSIMPGKVNPVMAELINQIAFQVIGNDHTICLASEAGQLEL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEE
NVMEPVLVFNLLQSISIMNNGFRSFTDHCVAGIEANEKRLKAYVEKSAGVITAVNPHLGY
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEECCCCCH
EAAARIAREAILTGQSVRDLCLQNDVLTEEELDLILNPYEMTKPGIAGAELLNRD
HHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHEECCHHHCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1711029; 8969508; 9384377 [H]