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The map label for this gene is srlB [H]

Identifier: 52787543

GI number: 52787543

Start: 3691630

End: 3692001

Strand: Reverse

Name: srlB [H]

Synonym: BLi03864

Alternate gene names: 52787543

Gene position: 3692001-3691630 (Counterclockwise)

Preceding gene: 52787544

Following gene: 52787542

Centisome position: 87.43

GC content: 43.01

Gene sequence:

>372_bases
ATGGTCATGCTTTATGAAACAAAAATAAACAGAATCGGAGCTCAAGCAGCGGCGTTTTCAGATGAAAAGATGCTCATATT
ATTTGGTGAAGGTGCTCCGGATGAATTGAAAGATTATTGTTTATCAATTGATATAAAGCCTGTAGAAGGCGACTTCAATG
AAGGCGATGAGCTCATACTGGGCGGCGAGACCTACAAAATTACAGCCGTTGGCGGAGCCGTTCGTCAAAACTTGGCGAAT
CTCGGGCATATTACGCTAAAATTTGACGGATCAGTTTCAGCCGAACTGCCGGGAACACTGTACCTTGAACGAAAAGAACA
ACTGCCGGAGGTTTCAGCAGGAGACTGTATTCAAGTTAAAAGATTCTCATAA

Upstream 100 bases:

>100_bases
GTATTCGCGTTTTCTCACGGGGGCGCCAACCGTTTTTATTGCTTGGCTGGCCAGCTTTGGCATGTATGACAGCTGAGGGT
TTTCTTAAGGAGGGAGAATA

Downstream 100 bases:

>100_bases
GGAGCGGGTGCAATATGGAGATGAATAAAGGAAAAGTGGCATTGATATGCGGCGGCGGCCAAAGTCTCGGAGAAGCGCTG
TCCTACCGGATGGCAGAGGC

Product: hypothetical protein

Products: protein histidine; sugar phosphate

Alternate protein names: EIIA-Gut; EIII-Gut; PTS system glucitol/sorbitol-specific EIIA component [H]

Number of amino acids: Translated: 123; Mature: 123

Protein sequence:

>123_residues
MVMLYETKINRIGAQAAAFSDEKMLILFGEGAPDELKDYCLSIDIKPVEGDFNEGDELILGGETYKITAVGGAVRQNLAN
LGHITLKFDGSVSAELPGTLYLERKEQLPEVSAGDCIQVKRFS

Sequences:

>Translated_123_residues
MVMLYETKINRIGAQAAAFSDEKMLILFGEGAPDELKDYCLSIDIKPVEGDFNEGDELILGGETYKITAVGGAVRQNLAN
LGHITLKFDGSVSAELPGTLYLERKEQLPEVSAGDCIQVKRFS
>Mature_123_residues
MVMLYETKINRIGAQAAAFSDEKMLILFGEGAPDELKDYCLSIDIKPVEGDFNEGDELILGGETYKITAVGGAVRQNLAN
LGHITLKFDGSVSAELPGTLYLERKEQLPEVSAGDCIQVKRFS

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG3731

COG function: function code G; Phosphotransferase system sorbitol-specific component IIA

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-5 domain [H]

Homologues:

Organism=Escherichia coli, GI1789056, Length=102, Percent_Identity=43.1372549019608, Blast_Score=89, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004716
- InterPro:   IPR018454
- ProDom:   PD015842 [H]

Pfam domain/function: PF03829 PTSIIA_gutA [H]

EC number: 2.7.1.69

Molecular weight: Translated: 13398; Mature: 13398

Theoretical pI: Translated: 4.31; Mature: 4.31

Prosite motif: PS51097 PTS_EIIA_TYPE_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVMLYETKINRIGAQAAAFSDEKMLILFGEGAPDELKDYCLSIDIKPVEGDFNEGDELIL
CEEEEEECCCCCCCCEEECCCCCEEEEECCCCCHHHHCEEEEEEEEECCCCCCCCCEEEE
GGETYKITAVGGAVRQNLANLGHITLKFDGSVSAELPGTLYLERKEQLPEVSAGDCIQVK
CCCEEEEEEECHHHHHHHHCCCEEEEEECCCCCCCCCCEEEEECCCCCCCCCCCCEEEEE
RFS
ECC
>Mature Secondary Structure
MVMLYETKINRIGAQAAAFSDEKMLILFGEGAPDELKDYCLSIDIKPVEGDFNEGDELIL
CEEEEEECCCCCCCCEEECCCCCEEEEECCCCCHHHHCEEEEEEEEECCCCCCCCCEEEE
GGETYKITAVGGAVRQNLANLGHITLKFDGSVSAELPGTLYLERKEQLPEVSAGDCIQVK
CCCEEEEEEECHHHHHHHHCCCEEEEEECCCCCCCCCCEEEEECCCCCCCCCCCCEEEEE
RFS
ECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferases; Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3553176; 1630305; 9205837; 9278503 [H]