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The map label for this gene is fruK [H]

Identifier: 52787239

GI number: 52787239

Start: 3394762

End: 3395676

Strand: Direct

Name: fruK [H]

Synonym: BLi03549

Alternate gene names: 52787239

Gene position: 3394762-3395676 (Clockwise)

Preceding gene: 52787238

Following gene: 52787240

Centisome position: 80.39

GC content: 47.21

Gene sequence:

>915_bases
ATGATCTATACATGCACGATGAACACCGCAGTCGACTTATTCGTTGAAACCGACGAGCTGCTTCCCGATGTTGTCAACCG
TACTAAAGATGAAGATTATCAGCCAAACGGAAAAGGCGTCAATATTTCATTTATGCTCAAGCGCCTTGGTCTTGACAATA
CCGCGCTTGGCTTTACAGGCGGATTCACCGGGCGTTTTATTGAAAATGAACTGCTGAATGAAAAAATCGTCACAGACTTT
GTCGAAGTGGAAGGCATCTCACGGATCAATATTTTTATCAATTCAACCCGGGAATTCAAGATTGTAAACAAAGGACCGCT
CATATCTGAAGCCCGAAAACAGGAGCTGATCAACAAAATCCGTCAAATCCCGCCGGGCCGCTTCTTATTTATTTCAGGCA
GTCTTCCGAGAGGAGTCCCTGATGACATGTACCTTTCGCTTTCCGAGATTGCCGCCGAAAACAAACTGAAATTGATCTTG
GATATCAGTTCTCCCGTCCTATTAGATTGTCTTTCATACCATCCTTACCTGATTAAGCCGAATGAACAGGAGCTTGCCGC
ATTTTTCAGCCGGAAGAAAGCGCTGTCCGAAGAAGAAATCATCCATTGCGGAAAGGAACTTCTCGACCGCGGCGCTGAAA
GGGTTCTCATCTCCCGCGGCAAAGACGGAGCCCTTTATTTGGACCGCCAAAGAACATTAAAAGCGACCGCCGCTAAAGGC
AAAGTTGTCAATACAGCATGTGCCGGCGATGCCATGCTTGCCGTATTCGTCGGAAAACAGGAGCTCGGCTGTTCCGTTCC
TGAAGCACTCAGGCACGCCTCTGCCGCCGGTTCCTTGACGGCATTCTCGAAAGGACTCGGTGATGTGTCCAAACTCGATC
AAACAGCTTCACAAATTCAAATCACTCATTTATAA

Upstream 100 bases:

>100_bases
GCACCGCTCATAGCTGAAAACCTTTACACAACCTGTAAACATCCCCCCTTTCATCTGTGACATAATGTCTGTGAAGCCAC
ATATAAAGGGGGGATACAGC

Downstream 100 bases:

>100_bases
AGGGGGTTTTCTTATGAAAAAGTTCAAAATCATCGCGGCAACCGGCTGTCCAACAGGGATTGCCCATACATTTATGGCTG
AAGAGGCCTTAAAACAGGCT

Product: hypothetical protein

Products: NA

Alternate protein names: Fructose 1-phosphate kinase [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MIYTCTMNTAVDLFVETDELLPDVVNRTKDEDYQPNGKGVNISFMLKRLGLDNTALGFTGGFTGRFIENELLNEKIVTDF
VEVEGISRINIFINSTREFKIVNKGPLISEARKQELINKIRQIPPGRFLFISGSLPRGVPDDMYLSLSEIAAENKLKLIL
DISSPVLLDCLSYHPYLIKPNEQELAAFFSRKKALSEEEIIHCGKELLDRGAERVLISRGKDGALYLDRQRTLKATAAKG
KVVNTACAGDAMLAVFVGKQELGCSVPEALRHASAAGSLTAFSKGLGDVSKLDQTASQIQITHL

Sequences:

>Translated_304_residues
MIYTCTMNTAVDLFVETDELLPDVVNRTKDEDYQPNGKGVNISFMLKRLGLDNTALGFTGGFTGRFIENELLNEKIVTDF
VEVEGISRINIFINSTREFKIVNKGPLISEARKQELINKIRQIPPGRFLFISGSLPRGVPDDMYLSLSEIAAENKLKLIL
DISSPVLLDCLSYHPYLIKPNEQELAAFFSRKKALSEEEIIHCGKELLDRGAERVLISRGKDGALYLDRQRTLKATAAKG
KVVNTACAGDAMLAVFVGKQELGCSVPEALRHASAAGSLTAFSKGLGDVSKLDQTASQIQITHL
>Mature_304_residues
MIYTCTMNTAVDLFVETDELLPDVVNRTKDEDYQPNGKGVNISFMLKRLGLDNTALGFTGGFTGRFIENELLNEKIVTDF
VEVEGISRINIFINSTREFKIVNKGPLISEARKQELINKIRQIPPGRFLFISGSLPRGVPDDMYLSLSEIAAENKLKLIL
DISSPVLLDCLSYHPYLIKPNEQELAAFFSRKKALSEEEIIHCGKELLDRGAERVLISRGKDGALYLDRQRTLKATAAKG
KVVNTACAGDAMLAVFVGKQELGCSVPEALRHASAAGSLTAFSKGLGDVSKLDQTASQIQITHL

Specific function: Key control step of glycolysis. [C]

COG id: COG1105

COG function: function code G; Fructose-1-phosphate kinase and related fructose-6-phosphate kinase (PfkB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family [H]

Homologues:

Organism=Escherichia coli, GI1788017, Length=289, Percent_Identity=28.719723183391, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1788493, Length=263, Percent_Identity=29.277566539924, Blast_Score=91, Evalue=6e-20,

Paralogues:

None

Copy number: 496 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022463
- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR017583 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: =2.7.1.56 [H]

Molecular weight: Translated: 33465; Mature: 33465

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYTCTMNTAVDLFVETDELLPDVVNRTKDEDYQPNGKGVNISFMLKRLGLDNTALGFTG
CEEEEECCCEEEEEEECHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHHCCCCCCEEECC
GFTGRFIENELLNEKIVTDFVEVEGISRINIFINSTREFKIVNKGPLISEARKQELINKI
CCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCEEEEEECCCCCHHHHHHHHHHHHH
RQIPPGRFLFISGSLPRGVPDDMYLSLSEIAAENKLKLILDISSPVLLDCLSYHPYLIKP
HHCCCCCEEEEECCCCCCCCHHHHEEHHHHCCCCCEEEEEECCCHHHHHHHHCCCEEECC
NEQELAAFFSRKKALSEEEIIHCGKELLDRGAERVLISRGKDGALYLDRQRTLKATAAKG
CHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHEEEEECCCCCCEEEECCHHHHHHHCCC
KVVNTACAGDAMLAVFVGKQELGCSVPEALRHASAAGSLTAFSKGLGDVSKLDQTASQIQ
CEEEEECCCCEEEEEEECCHHHCCCHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHEE
ITHL
EECC
>Mature Secondary Structure
MIYTCTMNTAVDLFVETDELLPDVVNRTKDEDYQPNGKGVNISFMLKRLGLDNTALGFTG
CEEEEECCCEEEEEEECHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHHCCCCCCEEECC
GFTGRFIENELLNEKIVTDFVEVEGISRINIFINSTREFKIVNKGPLISEARKQELINKI
CCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCEEEEEECCCCCHHHHHHHHHHHHH
RQIPPGRFLFISGSLPRGVPDDMYLSLSEIAAENKLKLILDISSPVLLDCLSYHPYLIKP
HHCCCCCEEEEECCCCCCCCHHHHEEHHHHCCCCCEEEEEECCCHHHHHHHHCCCEEECC
NEQELAAFFSRKKALSEEEIIHCGKELLDRGAERVLISRGKDGALYLDRQRTLKATAAKG
CHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHEEEEECCCCCCEEEECCHHHHHHHCCC
KVVNTACAGDAMLAVFVGKQELGCSVPEALRHASAAGSLTAFSKGLGDVSKLDQTASQIQ
CEEEEECCCCEEEEEEECCHHHCCCHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHEE
ITHL
EECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]