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The map label for this gene is fruA [H]

Identifier: 52787240

GI number: 52787240

Start: 3395690

End: 3397090

Strand: Direct

Name: fruA [H]

Synonym: BLi03550

Alternate gene names: 52787240

Gene position: 3395690-3397090 (Clockwise)

Preceding gene: 52787239

Following gene: 52787241

Centisome position: 80.42

GC content: 50.11

Gene sequence:

>1401_bases
ATGAAAAAGTTCAAAATCATCGCGGCAACCGGCTGTCCAACAGGGATTGCCCATACATTTATGGCTGAAGAGGCCTTAAA
ACAGGCTGCCGCCAAACTGGGTGTGGAAATCAAAGTCGAAACACACGGGCAAATCGGCGTTCAAAACGAACTATCGCCAG
AGGACATTAAACAAGCCGACGGGGTCATTGTCGCGGCAGACAAAGATGTAGGCGCAGAGCGTTTTGCAGGCAAACGTGTC
CTTGACGTCCCGGTTGCGCGGGGCATCCGGGATGCGGAATCTTTAATTCGGACGCTGATGAACGGCGAAGCGCCGATTTA
CCGCGGACAAACTGCGGCAAAAACAGAGGATGAACCAAAAACAGGCGAATCCGCTTCCATCGGCCGTAAAATATATAAAC
ATTTGATGAACGGGGTTTCCCACATGCTCCCATTTGTCGTCGGCGGAGGGGTTTTGATTGCACTTTCCTTTTTATTCGGC
ATCCATTCAGCGGATCCTGAACATCCAAGCTACAACGCTTTTGCCGAATTGCTGAATAAAACGGGCGCATTCGGATTCCA
GCTGATGGTTCCGATTTTATCCGCCTATATCGCCGATTCAATGGCAAAACGCCCCGGCCTGATTGTCGGTTTTATCGGCG
GCATGATCGCCAGCACGGGCGGAGCGGGTTTTCTGGGCGGCATCGTATCCGGTTTTTTGGCGGGACTGATCATTTACGGC
CTCTCGCATGCACTTAAAAAAATGCCTCAGTCACTTGAAGGACTAAAAGCAATTTTTCTTTATCCGGTTATCGGCATTTT
TCTGATCGGTGCGGTCATGTTTTTCCTCGTCGAACCGATGACGGCCATTAATGAGGGGATGAAGGGCTTTTTAGCCGACT
TTCAAGGGGCGAACCCAATCATTCTCGGCTTGATCATCGGCTGTATGAGCGCATTTGACATGGGCGGCCCGGTCAATAAA
GCCGCTTATGTCACCGGTACTGCTCTTCTCGCTGAAGGGAACCAATATTTCATGGCAGGCGTCTCCGCCGCGTGCATCAC
ACCGCCGCTCGTTATTGCGTTTGCCACATTGCTTTTCCGCAAATATTTCAGCCAGCAGGACCGAAATGCAGGACTTGTCA
ATTTTATTCTCGGCGCCACTCATATTACTGAAGGCGCTATCCCGTTCGCCGCAAAGAAACCAATCCCTGTCATTCCGATT
TTGATGATCGGCTCTTCGGTTTCGGCAATATTAACATATTCATTCGCAGTTCAAGTACCTGCCCCGCACGGCGGATTCCT
TGTACTTCCCGTTGTAACAGGAGCCTTTCAATGGGTGCTGTCGATTATCATCGGATCGCTTATCGGTGCCTTCTTATACG
GCTTTTACCAAAAACGGACATATGAAAAAGAGAAAGGCTGA

Upstream 100 bases:

>100_bases
GGTTCCTTGACGGCATTCTCGAAAGGACTCGGTGATGTGTCCAAACTCGATCAAACAGCTTCACAAATTCAAATCACTCA
TTTATAAAGGGGGTTTTCTT

Downstream 100 bases:

>100_bases
TCAAACATGAAAACAGCAGCTTTAATCAAAAAAGAGCACATCTATCTACAAGAAACGCTATCTTCCCAAGCGGAAGCTTT
TGACCGGCTGGCTCAGATCG

Product: hypothetical protein

Products: NA

Alternate protein names: EIIABC-Fru; Fructose-specific phosphotransferase enzyme IIA component; EII-Fru; PTS system fructose-specific EIIA component; Fructose-specific phosphotransferase enzyme IIB component; EIII-Fru; PTS system fructose-specific EIIB component; Fructose permease IIC component; PTS system fructose-specific EIIC component [H]

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MKKFKIIAATGCPTGIAHTFMAEEALKQAAAKLGVEIKVETHGQIGVQNELSPEDIKQADGVIVAADKDVGAERFAGKRV
LDVPVARGIRDAESLIRTLMNGEAPIYRGQTAAKTEDEPKTGESASIGRKIYKHLMNGVSHMLPFVVGGGVLIALSFLFG
IHSADPEHPSYNAFAELLNKTGAFGFQLMVPILSAYIADSMAKRPGLIVGFIGGMIASTGGAGFLGGIVSGFLAGLIIYG
LSHALKKMPQSLEGLKAIFLYPVIGIFLIGAVMFFLVEPMTAINEGMKGFLADFQGANPIILGLIIGCMSAFDMGGPVNK
AAYVTGTALLAEGNQYFMAGVSAACITPPLVIAFATLLFRKYFSQQDRNAGLVNFILGATHITEGAIPFAAKKPIPVIPI
LMIGSSVSAILTYSFAVQVPAPHGGFLVLPVVTGAFQWVLSIIIGSLIGAFLYGFYQKRTYEKEKG

Sequences:

>Translated_466_residues
MKKFKIIAATGCPTGIAHTFMAEEALKQAAAKLGVEIKVETHGQIGVQNELSPEDIKQADGVIVAADKDVGAERFAGKRV
LDVPVARGIRDAESLIRTLMNGEAPIYRGQTAAKTEDEPKTGESASIGRKIYKHLMNGVSHMLPFVVGGGVLIALSFLFG
IHSADPEHPSYNAFAELLNKTGAFGFQLMVPILSAYIADSMAKRPGLIVGFIGGMIASTGGAGFLGGIVSGFLAGLIIYG
LSHALKKMPQSLEGLKAIFLYPVIGIFLIGAVMFFLVEPMTAINEGMKGFLADFQGANPIILGLIIGCMSAFDMGGPVNK
AAYVTGTALLAEGNQYFMAGVSAACITPPLVIAFATLLFRKYFSQQDRNAGLVNFILGATHITEGAIPFAAKKPIPVIPI
LMIGSSVSAILTYSFAVQVPAPHGGFLVLPVVTGAFQWVLSIIIGSLIGAFLYGFYQKRTYEKEKG
>Mature_466_residues
MKKFKIIAATGCPTGIAHTFMAEEALKQAAAKLGVEIKVETHGQIGVQNELSPEDIKQADGVIVAADKDVGAERFAGKRV
LDVPVARGIRDAESLIRTLMNGEAPIYRGQTAAKTEDEPKTGESASIGRKIYKHLMNGVSHMLPFVVGGGVLIALSFLFG
IHSADPEHPSYNAFAELLNKTGAFGFQLMVPILSAYIADSMAKRPGLIVGFIGGMIASTGGAGFLGGIVSGFLAGLIIYG
LSHALKKMPQSLEGLKAIFLYPVIGIFLIGAVMFFLVEPMTAINEGMKGFLADFQGANPIILGLIIGCMSAFDMGGPVNK
AAYVTGTALLAEGNQYFMAGVSAACITPPLVIAFATLLFRKYFSQQDRNAGLVNFILGATHITEGAIPFAAKKPIPVIPI
LMIGSSVSAILTYSFAVQVPAPHGGFLVLPVVTGAFQWVLSIIIGSLIGAFLYGFYQKRTYEKEKG

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1299

COG function: function code G; Phosphotransferase system, fructose-specific IIC component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788492, Length=459, Percent_Identity=41.3943355119826, Blast_Score=328, Evalue=6e-91,
Organism=Escherichia coli, GI1786951, Length=474, Percent_Identity=32.0675105485232, Blast_Score=226, Evalue=3e-60,
Organism=Escherichia coli, GI87082348, Length=464, Percent_Identity=32.3275862068966, Blast_Score=225, Evalue=6e-60,
Organism=Escherichia coli, GI1790386, Length=320, Percent_Identity=37.8125, Blast_Score=202, Evalue=4e-53,
Organism=Escherichia coli, GI1788729, Length=397, Percent_Identity=25.6926952141058, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1790387, Length=95, Percent_Identity=49.4736842105263, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI1788730, Length=97, Percent_Identity=40.2061855670103, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- InterPro:   IPR004715
- InterPro:   IPR003353
- InterPro:   IPR006327 [H]

Pfam domain/function: PF00359 PTS_EIIA_2; PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 49238; Mature: 49238

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: PS51099 PTS_EIIB_TYPE_2 ; PS51104 PTS_EIIC_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFKIIAATGCPTGIAHTFMAEEALKQAAAKLGVEIKVETHGQIGVQNELSPEDIKQAD
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHC
GVIVAADKDVGAERFAGKRVLDVPVARGIRDAESLIRTLMNGEAPIYRGQTAAKTEDEPK
CEEEEECCCCCHHHHCCCCEEECHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
TGESASIGRKIYKHLMNGVSHMLPFVVGGGVLIALSFLFGIHSADPEHPSYNAFAELLNK
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
TGAFGFQLMVPILSAYIADSMAKRPGLIVGFIGGMIASTGGAGFLGGIVSGFLAGLIIYG
CCCCHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
LSHALKKMPQSLEGLKAIFLYPVIGIFLIGAVMFFLVEPMTAINEGMKGFLADFQGANPI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHH
ILGLIIGCMSAFDMGGPVNKAAYVTGTALLAEGNQYFMAGVSAACITPPLVIAFATLLFR
HHHHHHHHHHHHCCCCCCCCCHHEEHHHEEECCCEEEECCCHHHHHCHHHHHHHHHHHHH
KYFSQQDRNAGLVNFILGATHITEGAIPFAAKKPIPVIPILMIGSSVSAILTYSFAVQVP
HHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEHHHHHCCCHHHHHHHHEEEEEC
APHGGFLVLPVVTGAFQWVLSIIIGSLIGAFLYGFYQKRTYEKEKG
CCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKKFKIIAATGCPTGIAHTFMAEEALKQAAAKLGVEIKVETHGQIGVQNELSPEDIKQAD
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHC
GVIVAADKDVGAERFAGKRVLDVPVARGIRDAESLIRTLMNGEAPIYRGQTAAKTEDEPK
CEEEEECCCCCHHHHCCCCEEECHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
TGESASIGRKIYKHLMNGVSHMLPFVVGGGVLIALSFLFGIHSADPEHPSYNAFAELLNK
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
TGAFGFQLMVPILSAYIADSMAKRPGLIVGFIGGMIASTGGAGFLGGIVSGFLAGLIIYG
CCCCHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
LSHALKKMPQSLEGLKAIFLYPVIGIFLIGAVMFFLVEPMTAINEGMKGFLADFQGANPI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHH
ILGLIIGCMSAFDMGGPVNKAAYVTGTALLAEGNQYFMAGVSAACITPPLVIAFATLLFR
HHHHHHHHHHHHCCCCCCCCCHHEEHHHEEECCCEEEECCCHHHHHCHHHHHHHHHHHHH
KYFSQQDRNAGLVNFILGATHITEGAIPFAAKKPIPVIPILMIGSSVSAILTYSFAVQVP
HHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEHHHHHCCCHHHHHHHHEEEEEC
APHGGFLVLPVVTGAFQWVLSIIIGSLIGAFLYGFYQKRTYEKEKG
CCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]