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The map label for this gene is rph

Identifier: 52786716

GI number: 52786716

Start: 2867507

End: 2868247

Strand: Reverse

Name: rph

Synonym: BLi02985

Alternate gene names: 52786716

Gene position: 2868247-2867507 (Counterclockwise)

Preceding gene: 52786717

Following gene: 52786715

Centisome position: 67.93

GC content: 52.63

Gene sequence:

>741_bases
ATGAGATACGATGGAAGAAAAAACAATGAACTGCGCCCTGTCACAATGGACCTCGACTTTATTACGCATCCGGAAGGATC
GGTCCTGATTACAGTCGGCGGGACAAAGGTGATATGCAACGCGTCTGTGGAAGACCGCGTACCGCCCTTTTTGAGAGGAG
AAGGGAAGGGCTGGATCACCGCGGAATACAGCATGCTGCCGCGGGCCACGAACCAAAGGACGATCAGGGAATCGTCAAAA
GGGAAAATCTCCGGGCGGACGATGGAAATCCAACGGCTGATCGGACGAGCTCTTCGGGCGGTTGTTGATTTGGAAAAGCT
CGGTGAGCGGACGATCTGGATCGACTGCGACGTCATTCAGGCGGACGGAGGTACGAGAACTGCGTCGATTACCGGAGCAT
TCACCGCCATGGCGCTTGCCGTCGGAAGACTCGTCGAAAAAGGCGCGCTGAAGGAGATGCCGATCACCGACTTTTTGGCT
GCCACTTCAGTGGGCATCGATAAGGAAAAAGGATTGATCCTTGATTTGAACTACCAGGAAGATTCTGCAGCTGAGGTTGA
TATGAACGTCGTCATGACAGGCGAAGGCCGCTTTGTCGAACTTCAGGGAACAGGAGAGGAAGCGACATTCTCAAGAAGTG
AACTGGACGGGCTTTTAGAGCTGGCTGAAAAAGGGATCGCCGAACTCCTCGAAAAGCAGAAAGAAGTGCTCGGCGACGTT
GCATTATCTATTCAACAATAA

Upstream 100 bases:

>100_bases
AAAAGAGGTGAGCGATCAAAGCTGCCTCTTTCTTTATTCAGCTTTTTGGAAGAGCGGAATGATTGTGAAAGGTTTATGTA
CATACAATCGGAGGTAAAAA

Downstream 100 bases:

>100_bases
CATGAGAAAGGCTGATACACGATGAAGGAAGTCATCATTGCCACGAAAAACGAAGGGAAAGTCAGGGAATTCAAAGCGAT
GCTTGAACCGAGGGGCTACG

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MRYDGRKNNELRPVTMDLDFITHPEGSVLITVGGTKVICNASVEDRVPPFLRGEGKGWITAEYSMLPRATNQRTIRESSK
GKISGRTMEIQRLIGRALRAVVDLEKLGERTIWIDCDVIQADGGTRTASITGAFTAMALAVGRLVEKGALKEMPITDFLA
ATSVGIDKEKGLILDLNYQEDSAAEVDMNVVMTGEGRFVELQGTGEEATFSRSELDGLLELAEKGIAELLEKQKEVLGDV
ALSIQQ

Sequences:

>Translated_246_residues
MRYDGRKNNELRPVTMDLDFITHPEGSVLITVGGTKVICNASVEDRVPPFLRGEGKGWITAEYSMLPRATNQRTIRESSK
GKISGRTMEIQRLIGRALRAVVDLEKLGERTIWIDCDVIQADGGTRTASITGAFTAMALAVGRLVEKGALKEMPITDFLA
ATSVGIDKEKGLILDLNYQEDSAAEVDMNVVMTGEGRFVELQGTGEEATFSRSELDGLLELAEKGIAELLEKQKEVLGDV
ALSIQQ
>Mature_246_residues
MRYDGRKNNELRPVTMDLDFITHPEGSVLITVGGTKVICNASVEDRVPPFLRGEGKGWITAEYSMLPRATNQRTIRESSK
GKISGRTMEIQRLIGRALRAVVDLEKLGERTIWIDCDVIQADGGTRTASITGAFTAMALAVGRLVEKGALKEMPITDFLA
ATSVGIDKEKGLILDLNYQEDSAAEVDMNVVMTGEGRFVELQGTGEEATFSRSELDGLLELAEKGIAELLEKQKEVLGDV
ALSIQQ

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family

Homologues:

Organism=Escherichia coli, GI157672248, Length=212, Percent_Identity=60.377358490566, Blast_Score=256, Evalue=8e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNPH_BACLD (Q65GG2)

Other databases:

- EMBL:   CP000002
- EMBL:   AE017333
- RefSeq:   YP_080129.2
- RefSeq:   YP_092545.1
- HSSP:   P28619
- ProteinModelPortal:   Q65GG2
- SMR:   Q65GG2
- STRING:   Q65GG2
- EnsemblBacteria:   EBBACT00000056374
- EnsemblBacteria:   EBBACT00000059632
- GeneID:   3028501
- GeneID:   3098495
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi02985
- KEGG:   bli:BL00312
- NMPDR:   fig|279010.5.peg.3334
- eggNOG:   COG0689
- GeneTree:   EBGT00050000001825
- HOGENOM:   HBG737187
- ProtClustDB:   PRK00173
- BioCyc:   BLIC279010-1:BLI02985-MONOMER
- HAMAP:   MF_00564
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336
- TIGRFAMs:   TIGR01966

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C; SSF55666 3_ExoRNase; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.56

Molecular weight: Translated: 26845; Mature: 26845

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYDGRKNNELRPVTMDLDFITHPEGSVLITVGGTKVICNASVEDRVPPFLRGEGKGWIT
CCCCCCCCCCCCEEEEEEEEEECCCCCEEEEECCCEEEEECCHHHCCCCCEECCCCEEEE
AEYSMLPRATNQRTIRESSKGKISGRTMEIQRLIGRALRAVVDLEKLGERTIWIDCDVIQ
EEHEECCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEE
ADGGTRTASITGAFTAMALAVGRLVEKGALKEMPITDFLAATSVGIDKEKGLILDLNYQE
CCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCC
DSAAEVDMNVVMTGEGRFVELQGTGEEATFSRSELDGLLELAEKGIAELLEKQKEVLGDV
CCCCEEEEEEEEECCCCEEEEECCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALSIQQ
HHEECC
>Mature Secondary Structure
MRYDGRKNNELRPVTMDLDFITHPEGSVLITVGGTKVICNASVEDRVPPFLRGEGKGWIT
CCCCCCCCCCCCEEEEEEEEEECCCCCEEEEECCCEEEEECCHHHCCCCCEECCCCEEEE
AEYSMLPRATNQRTIRESSKGKISGRTMEIQRLIGRALRAVVDLEKLGERTIWIDCDVIQ
EEHEECCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEE
ADGGTRTASITGAFTAMALAVGRLVEKGALKEMPITDFLAATSVGIDKEKGLILDLNYQE
CCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCC
DSAAEVDMNVVMTGEGRFVELQGTGEEATFSRSELDGLLELAEKGIAELLEKQKEVLGDV
CCCCEEEEEEEEECCCCEEEEECCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALSIQQ
HHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA