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The map label for this gene is ilvE [H]

Identifier: 52786694

GI number: 52786694

Start: 2851215

End: 2852129

Strand: Reverse

Name: ilvE [H]

Synonym: BLi02962

Alternate gene names: 52786694

Gene position: 2852129-2851215 (Counterclockwise)

Preceding gene: 52786696

Following gene: 52786693

Centisome position: 67.54

GC content: 48.42

Gene sequence:

>915_bases
ATGGGGGACCAGAAAGACCAGTGGATCTTCCTAAACGACAAACTCGTTAAAAAAGAAGACGCTAAAATATCAGTCTATGA
TCACGGATTTTTATACGGGGACGGCGTGTTTGAAGGGATCAGGGTATACGACGGCAACATCTTCAGAATGCAAGAGCACA
TGGACCGCCTCTACGATTCTGCGAGATCGATCATGCTGGAGATTCCATATCCACAGGAAGAACTGACACAGCACGTACTC
AAAACAGTCGAAAAAAACGGGCTGAAAGACGCTTACATCCGCTTGGTCGTTTCAAGAGGAGCAGGTGACCTCGGACTCGA
TCCAAACAATTGTTCAAACCCGAGTGTCATCATAATTGTCGAACCATTGGCAATATTCCCGAAACATTTATATGAAACGG
GGATTGACATTGTTACGGTTCCGACAAGAAGGAACAGACCCGATGTGCTGAGCCCTAAAGTAAAATCGCTGAACTACTTA
AACAATATTCTTGTCCGGATCGAGGCGCATATGGCGGGTGTGACGGAAGCGCTCATGCTCAATGATCAAGGCTATGTCGC
CGAAGGGTCTGCGGATAACGTATTTATTTATAAAAACGGAAAGCTCTTGACGCCTCCGGGCTATATCGGAGCGCTTGAAG
GAATCACCCGGAATGCCATCATCGAAATAGCGCGAGAGCTCGGCTATGAAGTGAAAGAAGAGCCGTTTACCCGCCATGAC
GTATACACAGCCGAGGAAGTGTTTTTAACCGGAACGGCTGCAGAAGTCATCGCGGTCGTAAAAGTTGACGGCCGCAAGAT
CGGGGACGGCAAACCGGGAGTCCACACAAACCGGATGCTTGAAAAGTTCCGCGAGCGCGTCGTCCGTGAAGGGTTAAAAG
TCAGCCTCAAAGATCAAAGCTTAAGTGTCAGCTGA

Upstream 100 bases:

>100_bases
TTCATCTTTCCCGGGAATGACAGCGGGAAAACGGGTTCCCGCATCATGAATTTTTAATCGTTTAAAGCGTTCATTTACAA
CAGAAAGGTGTGCTAGAGGG

Downstream 100 bases:

>100_bases
ATAAAGATAAACGTTGAAGAGGATAAGTAGCAGCGGAAACGCCGCCTGCAGAGAGCCGGGATTGCTGGGAACCGGTGACG
GCCTCCGCTTGTGAACTCCC

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT; Transaminase B [H]

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MGDQKDQWIFLNDKLVKKEDAKISVYDHGFLYGDGVFEGIRVYDGNIFRMQEHMDRLYDSARSIMLEIPYPQEELTQHVL
KTVEKNGLKDAYIRLVVSRGAGDLGLDPNNCSNPSVIIIVEPLAIFPKHLYETGIDIVTVPTRRNRPDVLSPKVKSLNYL
NNILVRIEAHMAGVTEALMLNDQGYVAEGSADNVFIYKNGKLLTPPGYIGALEGITRNAIIEIARELGYEVKEEPFTRHD
VYTAEEVFLTGTAAEVIAVVKVDGRKIGDGKPGVHTNRMLEKFRERVVREGLKVSLKDQSLSVS

Sequences:

>Translated_304_residues
MGDQKDQWIFLNDKLVKKEDAKISVYDHGFLYGDGVFEGIRVYDGNIFRMQEHMDRLYDSARSIMLEIPYPQEELTQHVL
KTVEKNGLKDAYIRLVVSRGAGDLGLDPNNCSNPSVIIIVEPLAIFPKHLYETGIDIVTVPTRRNRPDVLSPKVKSLNYL
NNILVRIEAHMAGVTEALMLNDQGYVAEGSADNVFIYKNGKLLTPPGYIGALEGITRNAIIEIARELGYEVKEEPFTRHD
VYTAEEVFLTGTAAEVIAVVKVDGRKIGDGKPGVHTNRMLEKFRERVVREGLKVSLKDQSLSVS
>Mature_303_residues
GDQKDQWIFLNDKLVKKEDAKISVYDHGFLYGDGVFEGIRVYDGNIFRMQEHMDRLYDSARSIMLEIPYPQEELTQHVLK
TVEKNGLKDAYIRLVVSRGAGDLGLDPNNCSNPSVIIIVEPLAIFPKHLYETGIDIVTVPTRRNRPDVLSPKVKSLNYLN
NILVRIEAHMAGVTEALMLNDQGYVAEGSADNVFIYKNGKLLTPPGYIGALEGITRNAIIEIARELGYEVKEEPFTRHDV
YTAEEVFLTGTAAEVIAVVKVDGRKIGDGKPGVHTNRMLEKFRERVVREGLKVSLKDQSLSVS

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=289, Percent_Identity=39.4463667820069, Blast_Score=204, Evalue=4e-54,
Organism=Escherichia coli, GI1787338, Length=245, Percent_Identity=28.1632653061224, Blast_Score=101, Evalue=7e-23,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 34105; Mature: 33973

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDQKDQWIFLNDKLVKKEDAKISVYDHGFLYGDGVFEGIRVYDGNIFRMQEHMDRLYDS
CCCCCCCEEEECCCEECCCCCEEEEEECCEEEECCHHCCEEEECCCEEEHHHHHHHHHHH
ARSIMLEIPYPQEELTQHVLKTVEKNGLKDAYIRLVVSRGAGDLGLDPNNCSNPSVIIIV
HCEEEEECCCCHHHHHHHHHHHHHHCCCHHHEEEEEEECCCCCCCCCCCCCCCCEEEEEE
EPLAIFPKHLYETGIDIVTVPTRRNRPDVLSPKVKSLNYLNNILVRIEAHMAGVTEALML
CCHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHEEEEHHHHCHHHEEEE
NDQGYVAEGSADNVFIYKNGKLLTPPGYIGALEGITRNAIIEIARELGYEVKEEPFTRHD
CCCCEEECCCCCEEEEEECCEEECCCCCHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCC
VYTAEEVFLTGTAAEVIAVVKVDGRKIGDGKPGVHTNRMLEKFRERVVREGLKVSLKDQS
CCEEHHEEEECCHHHEEEEEEECCEEECCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCC
LSVS
CCCC
>Mature Secondary Structure 
GDQKDQWIFLNDKLVKKEDAKISVYDHGFLYGDGVFEGIRVYDGNIFRMQEHMDRLYDS
CCCCCCEEEECCCEECCCCCEEEEEECCEEEECCHHCCEEEECCCEEEHHHHHHHHHHH
ARSIMLEIPYPQEELTQHVLKTVEKNGLKDAYIRLVVSRGAGDLGLDPNNCSNPSVIIIV
HCEEEEECCCCHHHHHHHHHHHHHHCCCHHHEEEEEEECCCCCCCCCCCCCCCCEEEEEE
EPLAIFPKHLYETGIDIVTVPTRRNRPDVLSPKVKSLNYLNNILVRIEAHMAGVTEALML
CCHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHEEEEHHHHCHHHEEEE
NDQGYVAEGSADNVFIYKNGKLLTPPGYIGALEGITRNAIIEIARELGYEVKEEPFTRHD
CCCCEEECCCCCEEEEEECCEEECCCCCHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCC
VYTAEEVFLTGTAAEVIAVVKVDGRKIGDGKPGVHTNRMLEKFRERVVREGLKVSLKDQS
CCEEHHEEEECCHHHEEEEEEECCEEECCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCC
LSVS
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]