Click here to switch to the map view.

The map label for this gene is rocG [H]

Identifier: 52786696

GI number: 52786696

Start: 2852858

End: 2854240

Strand: Reverse

Name: rocG [H]

Synonym: BLi02964

Alternate gene names: 52786696

Gene position: 2854240-2852858 (Counterclockwise)

Preceding gene: 52786698

Following gene: 52786694

Centisome position: 67.59

GC content: 50.83

Gene sequence:

>1383_bases
GTGCATACGCTAGAAAAAATGGAGCAAACAAATTTCCAGACGGCGAGAGATTATGTGACACAAGCATACGAGACAGTACA
GAAGCGAAATTTTTACGAAAGCGAATTTCTTCAAGCTGTAAAGGAAATATTTGATTCCCTTGTCCCTGTATTGGCAAGGC
ATCCAAAGTATATCGAACACCGCATTCTTGAGAGGATCGCAGAGCCGGAACGGATGATCACCTTCAGGGTGCCGTGGGTC
GATGATGAAGGCAATATCCGGGTTAACCGAGGGTTCCGGGTTCAATTTAACAGTGCAATCGGTCCGTATAAAGGCGGCAT
CCGCTTTCACCCTTCTGTGAACGCGAGCATTATTAAATTTTTGGGTTTTGAGCAGATTTTTAAAAATTCTTTGACCGGAC
TGCCGATCGGAGGCGGAAAAGGCGGGGCTGATTTTGATCCGAAGGGCAAATCGGACAGGGAGATTATGAGTTTTACGCAG
AGCTTCATGAATGAACTGTACAGACATATCGGACCGGACACGGATATCCCTGCCGGCGATATTGGTGTCGGAGCAAGGGA
AGTCGGGTTTATGTTCGGACAGTATAAAAAGATTCGGGGCCGCTATGATGCAGGCGTGTTAACAGGCAAAGGCCTTGAAT
ACGGGGGCAGTTTAACGAGGAAAGAAGCGACAGGGTACGGTCTGGTTTATTTCGTGGAAGAAATGCTGAAGGATCAGGGG
ATGCGCTTTGAAAACAGCACCGTTGTCGTCTCCGGTTCAGGGAATGTGGCGCTGTACGCGATGGAAAAAGCCGCTCAATT
CGGTGCGAAGGTGGTGGCCTGCAGCGATTCTGACGGCTATGTCTATGACGAAAAAGGCATCTGTCTTGAGACGGTGAAGC
GGCTCAAAGAAGACGGGAACGGAAGGATTCGCGAGTATGTCAGCGAGCATCCGGAAGCACACTATTTCGAGGGATGTACC
GGCATTTGGTCTATTCCATGCGATATCGCGCTTCCGTGCGCGACCCAGAACGAAATTGACGAAGAGGCGGCCGAAGTGCT
CATTTCAAATGGGGTCAAAGCTGTCGGAGAAGGAGCAAATATGCCGTCTGAAGAGGGCGCCGTCAAACGCTTTTTGGATG
CGGGAGTTCTATTCGGACCGGCTAAGGCTGCAAATGCCGGCGGTGTAGCCGTTTCAGCGCTCGAAATGGCGCAGAACAGC
GCACGGCTTCACTGGACGGCGGAAGAAACGGATGCGAAGCTCAGGGCGATCATGGCTGATATTCACAAGAGAAGCGTTGA
AGCGGCTTCAGAATACGGACGGCCCGGAAATCTGCTCGACGGCTGCAATATAGCCGGATTTATCAAAGTGGCGGATGCGA
TGATCGCTCAGGGAGTCGTTTAA

Upstream 100 bases:

>100_bases
CCAGATTGATGTAAATGGGGCATTGGTTGTAAAATTTACCATGAGAGAAAATCTTACAATGTTCGTTCTGCTTTTATTTT
AGAAAGGGAGCTGAAAAAAG

Downstream 100 bases:

>100_bases
CGGTAAAAAGAGAGAGCATGATGCCCTCTCTTTTTTTGTGCTTTATTCATTGTCTCTGAATCCACCGGTAAAGGCGTGTA
CAGCCGGAACGGAAATTCCC

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: NADP-GDH [H]

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MHTLEKMEQTNFQTARDYVTQAYETVQKRNFYESEFLQAVKEIFDSLVPVLARHPKYIEHRILERIAEPERMITFRVPWV
DDEGNIRVNRGFRVQFNSAIGPYKGGIRFHPSVNASIIKFLGFEQIFKNSLTGLPIGGGKGGADFDPKGKSDREIMSFTQ
SFMNELYRHIGPDTDIPAGDIGVGAREVGFMFGQYKKIRGRYDAGVLTGKGLEYGGSLTRKEATGYGLVYFVEEMLKDQG
MRFENSTVVVSGSGNVALYAMEKAAQFGAKVVACSDSDGYVYDEKGICLETVKRLKEDGNGRIREYVSEHPEAHYFEGCT
GIWSIPCDIALPCATQNEIDEEAAEVLISNGVKAVGEGANMPSEEGAVKRFLDAGVLFGPAKAANAGGVAVSALEMAQNS
ARLHWTAEETDAKLRAIMADIHKRSVEAASEYGRPGNLLDGCNIAGFIKVADAMIAQGVV

Sequences:

>Translated_460_residues
MHTLEKMEQTNFQTARDYVTQAYETVQKRNFYESEFLQAVKEIFDSLVPVLARHPKYIEHRILERIAEPERMITFRVPWV
DDEGNIRVNRGFRVQFNSAIGPYKGGIRFHPSVNASIIKFLGFEQIFKNSLTGLPIGGGKGGADFDPKGKSDREIMSFTQ
SFMNELYRHIGPDTDIPAGDIGVGAREVGFMFGQYKKIRGRYDAGVLTGKGLEYGGSLTRKEATGYGLVYFVEEMLKDQG
MRFENSTVVVSGSGNVALYAMEKAAQFGAKVVACSDSDGYVYDEKGICLETVKRLKEDGNGRIREYVSEHPEAHYFEGCT
GIWSIPCDIALPCATQNEIDEEAAEVLISNGVKAVGEGANMPSEEGAVKRFLDAGVLFGPAKAANAGGVAVSALEMAQNS
ARLHWTAEETDAKLRAIMADIHKRSVEAASEYGRPGNLLDGCNIAGFIKVADAMIAQGVV
>Mature_460_residues
MHTLEKMEQTNFQTARDYVTQAYETVQKRNFYESEFLQAVKEIFDSLVPVLARHPKYIEHRILERIAEPERMITFRVPWV
DDEGNIRVNRGFRVQFNSAIGPYKGGIRFHPSVNASIIKFLGFEQIFKNSLTGLPIGGGKGGADFDPKGKSDREIMSFTQ
SFMNELYRHIGPDTDIPAGDIGVGAREVGFMFGQYKKIRGRYDAGVLTGKGLEYGGSLTRKEATGYGLVYFVEEMLKDQG
MRFENSTVVVSGSGNVALYAMEKAAQFGAKVVACSDSDGYVYDEKGICLETVKRLKEDGNGRIREYVSEHPEAHYFEGCT
GIWSIPCDIALPCATQNEIDEEAAEVLISNGVKAVGEGANMPSEEGAVKRFLDAGVLFGPAKAANAGGVAVSALEMAQNS
ARLHWTAEETDAKLRAIMADIHKRSVEAASEYGRPGNLLDGCNIAGFIKVADAMIAQGVV

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=353, Percent_Identity=32.8611898016997, Blast_Score=169, Evalue=5e-42,
Organism=Homo sapiens, GI4885281, Length=353, Percent_Identity=32.5779036827195, Blast_Score=165, Evalue=7e-41,
Organism=Escherichia coli, GI1788059, Length=438, Percent_Identity=62.3287671232877, Blast_Score=534, Evalue=1e-153,
Organism=Caenorhabditis elegans, GI17544676, Length=352, Percent_Identity=30.6818181818182, Blast_Score=142, Evalue=5e-34,
Organism=Saccharomyces cerevisiae, GI6324951, Length=455, Percent_Identity=48.7912087912088, Blast_Score=383, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6319256, Length=459, Percent_Identity=49.2374727668845, Blast_Score=382, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24649283, Length=351, Percent_Identity=31.6239316239316, Blast_Score=154, Evalue=2e-37,
Organism=Drosophila melanogaster, GI45553475, Length=375, Percent_Identity=30.1333333333333, Blast_Score=148, Evalue=6e-36,
Organism=Drosophila melanogaster, GI45549226, Length=375, Percent_Identity=30.1333333333333, Blast_Score=148, Evalue=7e-36,
Organism=Drosophila melanogaster, GI24649460, Length=286, Percent_Identity=31.1188811188811, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.4 [H]

Molecular weight: Translated: 50469; Mature: 50469

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHTLEKMEQTNFQTARDYVTQAYETVQKRNFYESEFLQAVKEIFDSLVPVLARHPKYIEH
CCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
RILERIAEPERMITFRVPWVDDEGNIRVNRGFRVQFNSAIGPYKGGIRFHPSVNASIIKF
HHHHHHCCCCCEEEEECCEECCCCCEEECCCEEEEECCCCCCCCCCEEECCCCCHHHHHH
LGFEQIFKNSLTGLPIGGGKGGADFDPKGKSDREIMSFTQSFMNELYRHIGPDTDIPAGD
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
IGVGAREVGFMFGQYKKIRGRYDAGVLTGKGLEYGGSLTRKEATGYGLVYFVEEMLKDQG
CCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCHHCCCCHHHHHHHHHHHHCC
MRFENSTVVVSGSGNVALYAMEKAAQFGAKVVACSDSDGYVYDEKGICLETVKRLKEDGN
CEEECCEEEEECCCCEEEEEEHHHHHCCCEEEEEECCCCEEECCCCCHHHHHHHHHHCCC
GRIREYVSEHPEAHYFEGCTGIWSIPCDIALPCATQNEIDEEAAEVLISNGVKAVGEGAN
CHHHHHHHCCCCCCHHCCCCCEEECCCCCEECCCCCHHHHHHHHHHHHHCCCHHHCCCCC
MPSEEGAVKRFLDAGVLFGPAKAANAGGVAVSALEMAQNSARLHWTAEETDAKLRAIMAD
CCCHHHHHHHHHHCCCEECCCCCCCCCCHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHH
IHKRSVEAASEYGRPGNLLDGCNIAGFIKVADAMIAQGVV
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MHTLEKMEQTNFQTARDYVTQAYETVQKRNFYESEFLQAVKEIFDSLVPVLARHPKYIEH
CCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
RILERIAEPERMITFRVPWVDDEGNIRVNRGFRVQFNSAIGPYKGGIRFHPSVNASIIKF
HHHHHHCCCCCEEEEECCEECCCCCEEECCCEEEEECCCCCCCCCCEEECCCCCHHHHHH
LGFEQIFKNSLTGLPIGGGKGGADFDPKGKSDREIMSFTQSFMNELYRHIGPDTDIPAGD
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
IGVGAREVGFMFGQYKKIRGRYDAGVLTGKGLEYGGSLTRKEATGYGLVYFVEEMLKDQG
CCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCHHCCCCHHHHHHHHHHHHCC
MRFENSTVVVSGSGNVALYAMEKAAQFGAKVVACSDSDGYVYDEKGICLETVKRLKEDGN
CEEECCEEEEECCCCEEEEEEHHHHHCCCEEEEEECCCCEEECCCCCHHHHHHHHHHCCC
GRIREYVSEHPEAHYFEGCTGIWSIPCDIALPCATQNEIDEEAAEVLISNGVKAVGEGAN
CHHHHHHHCCCCCCHHCCCCCEEECCCCCEECCCCCHHHHHHHHHHHHHCCCHHHCCCCC
MPSEEGAVKRFLDAGVLFGPAKAANAGGVAVSALEMAQNSARLHWTAEETDAKLRAIMAD
CCCHHHHHHHHHHCCCEECCCCCCCCCCHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHH
IHKRSVEAASEYGRPGNLLDGCNIAGFIKVADAMIAQGVV
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308576; 6373501; 9097039; 9278503; 9298646 [H]