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The map label for this gene is ruvA

Identifier: 52786633

GI number: 52786633

Start: 2791156

End: 2791761

Strand: Reverse

Name: ruvA

Synonym: BLi02901

Alternate gene names: 52786633

Gene position: 2791761-2791156 (Counterclockwise)

Preceding gene: 52786634

Following gene: 52786632

Centisome position: 66.11

GC content: 45.21

Gene sequence:

>606_bases
TTGATCGAATTCGTAAAAGGGACGATTGATTATGTATCGCCCCAATATATTGTCATTGAAAACGGCGGGATCGGCTATCA
GATCTTCACGCCAAATCCGTTTATTTATAAGAAAAACAGCAAAGAAACAATCTATACATACCATTATGTAAGAGAAGACA
CGAATGCGCTGTACGGCTTTTCGACAAGGGAAGAAAAAATGCTGTTTACGAAAATGCTGAATGTTACGGGGATCGGCCCA
AAAGGAGCGCTTGCGATCCTCGCTTCCGGCGATCCGGGAGCGGTGATTGAAGCGATCGAGAATGAGGACGAAGCATTTCT
CGTCAAATTTCCCGGCGTAGGCAAAAAAACGGCAAGGCAGATCATCCTTGACCTGAAAGGGAAGCTTGCGGATGCCGTTC
CTGAAATCGCTGCCGATCTGTTTAACCATGAAGAGCGGGAGCAAATTCATGAAGCGGCAACGGCTCTTGAGGAAGCGCTT
GAAGCATTGAGGGCGCTCGGCTATTCCGATCGGGAAATTAAAAAAGTCCAGCCTCATTTGAAAAAGGAAGAGGCGCTTTC
GACAGATCAATACGTTAAAAAAGCATTGCAAAAATTGTTAAAGTAA

Upstream 100 bases:

>100_bases
TGCAGACAGAAGCTTTTTTAGCGAACATATGTTAACTTTTTCATTCTAGCTTTGCCTGTTTTGTGTTACAATGAAGAGCA
GTCAAAGAGGTGAATGAACG

Downstream 100 bases:

>100_bases
GGTGATATCATGGATGAGCGGCTTGTATCAAGTGAACTAGACAACCATGAATCAGCGATTGAGCAAAGTCTGCGTCCGCA
AAGACTTGCTCAGTATATCG

Product: Holliday junction DNA helicase RuvA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MIEFVKGTIDYVSPQYIVIENGGIGYQIFTPNPFIYKKNSKETIYTYHYVREDTNALYGFSTREEKMLFTKMLNVTGIGP
KGALAILASGDPGAVIEAIENEDEAFLVKFPGVGKKTARQIILDLKGKLADAVPEIAADLFNHEEREQIHEAATALEEAL
EALRALGYSDREIKKVQPHLKKEEALSTDQYVKKALQKLLK

Sequences:

>Translated_201_residues
MIEFVKGTIDYVSPQYIVIENGGIGYQIFTPNPFIYKKNSKETIYTYHYVREDTNALYGFSTREEKMLFTKMLNVTGIGP
KGALAILASGDPGAVIEAIENEDEAFLVKFPGVGKKTARQIILDLKGKLADAVPEIAADLFNHEEREQIHEAATALEEAL
EALRALGYSDREIKKVQPHLKKEEALSTDQYVKKALQKLLK
>Mature_201_residues
MIEFVKGTIDYVSPQYIVIENGGIGYQIFTPNPFIYKKNSKETIYTYHYVREDTNALYGFSTREEKMLFTKMLNVTGIGP
KGALAILASGDPGAVIEAIENEDEAFLVKFPGVGKKTARQIILDLKGKLADAVPEIAADLFNHEEREQIHEAATALEEAL
EALRALGYSDREIKKVQPHLKKEEALSTDQYVKKALQKLLK

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG0632

COG function: function code L; Holliday junction resolvasome, DNA-binding subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvA family

Homologues:

Organism=Escherichia coli, GI1788168, Length=208, Percent_Identity=33.1730769230769, Blast_Score=115, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVA_BACLD (Q65GP5)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_080044.1
- RefSeq:   YP_092462.1
- ProteinModelPortal:   Q65GP5
- SMR:   Q65GP5
- STRING:   Q65GP5
- EnsemblBacteria:   EBBACT00000057117
- EnsemblBacteria:   EBBACT00000060858
- GeneID:   3027343
- GeneID:   3098261
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi02901
- KEGG:   bli:BL01144
- NMPDR:   fig|279010.5.peg.3228
- eggNOG:   COG0632
- GeneTree:   EBGT00050000000364
- HOGENOM:   HBG635309
- OMA:   IATKEVK
- ProtClustDB:   PRK00116
- BioCyc:   BLIC279010-1:BLI02901-MONOMER
- BioCyc:   BLIC279010:BL01144-MONOMER
- HAMAP:   MF_00031
- InterPro:   IPR011114
- InterPro:   IPR013849
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000085
- InterPro:   IPR010994
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00278
- TIGRFAMs:   TIGR00084

Pfam domain/function: PF07499 RuvA_C; PF01330 RuvA_N; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like; SSF46929 RuvA_C-like

EC number: =3.6.4.12

Molecular weight: Translated: 22509; Mature: 22509

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEFVKGTIDYVSPQYIVIENGGIGYQIFTPNPFIYKKNSKETIYTYHYVREDTNALYGF
CCEEECCHHHHCCCCEEEEECCCEEEEEECCCCEEEECCCCCEEEEEEEEECCCCEEECC
STREEKMLFTKMLNVTGIGPKGALAILASGDPGAVIEAIENEDEAFLVKFPGVGKKTARQ
CCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHEECCCCCEEEEECCCCCHHHHHH
IILDLKGKLADAVPEIAADLFNHEEREQIHEAATALEEALEALRALGYSDREIKKVQPHL
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
KKEEALSTDQYVKKALQKLLK
HHHHHCCHHHHHHHHHHHHHC
>Mature Secondary Structure
MIEFVKGTIDYVSPQYIVIENGGIGYQIFTPNPFIYKKNSKETIYTYHYVREDTNALYGF
CCEEECCHHHHCCCCEEEEECCCEEEEEECCCCEEEECCCCCEEEEEEEEECCCCEEECC
STREEKMLFTKMLNVTGIGPKGALAILASGDPGAVIEAIENEDEAFLVKFPGVGKKTARQ
CCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHEECCCCCEEEEECCCCCHHHHHH
IILDLKGKLADAVPEIAADLFNHEEREQIHEAATALEEALEALRALGYSDREIKKVQPHL
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
KKEEALSTDQYVKKALQKLLK
HHHHHCCHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA