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The map label for this gene is ruvB

Identifier: 52786632

GI number: 52786632

Start: 2790142

End: 2791146

Strand: Reverse

Name: ruvB

Synonym: BLi02900

Alternate gene names: 52786632

Gene position: 2791146-2790142 (Counterclockwise)

Preceding gene: 52786633

Following gene: 52786631

Centisome position: 66.1

GC content: 52.74

Gene sequence:

>1005_bases
ATGGATGAGCGGCTTGTATCAAGTGAACTAGACAACCATGAATCAGCGATTGAGCAAAGTCTGCGTCCGCAAAGACTTGC
TCAGTATATCGGCCAGGAAAAAGTGAAAGATAACCTCAAGGTTTTCATCGAAGCTGCGAAAATGCGGGAGGAAACCCTTG
ACCATGTCTTATTGTACGGGCCGCCGGGATTGGGAAAAACGACGCTCGCCGCGATCATCGCCAACGAAATGGGAGTCAAT
CTCAGAACGACGTCAGGTCCGGCGATCGAGCGCCCCGGTGATTTGGCGGCGATATTAACGGCTCTGGAGCCAGGTGACGT
TTTATTTATAGACGAGATCCACCGCCTGCACCGTTCGATCGAAGAGGTGCTGTATCCTGCGATGGAAGATTTCTGCCTTG
ATATCGTGATCGGCAAAGGACCGTCGGCACGTTCTGTGAGGCTCGATCTTCCTCCTTTTACCCTCGTCGGCGCGACCACA
AGGGTTGGGCTTTTAACCGCGCCTCTCAGGGACAGGTTCGGCGTTCTGTCGAGGCTTGAGTATTACACGCGTGATGAGCT
TTCAGAGATCGTGATCAGAACGGCAGAGCTGTTTGAAGTCGACATCGATTCGCTCTCGGCTCTCGAAATCGCGAGACGGT
CAAGAGGGACGCCGCGGATTGCCAACAGGCTTTTAAGGAGGGTCAGGGATTTTGCCCAGGTGCTTGGAAACAGCTCCATC
ACAGAGGAAGTGGCGGTTGATGCGCTTGAACGCCTCCAGGTTGATAAACTCGGGCTTGACCATATCGACCGCAAGCTTTT
GATGGGCATGATCGAAAAATTTGGCGGCGGCCCCGTCGGCATCGATACGATTTCGGCTACTATCGGTGAGGAGTCTCACA
CGATTGAGGATGTGTATGAGCCGTATCTTCTTCAAATCGGTTTTATTCAAAGAACGCCGCGGGGGCGCATCGTAACCCCT
GATGTTTACAGCCACTTTAAAATGGAGGTTCCGAACCATGACTGA

Upstream 100 bases:

>100_bases
AATTAAAAAAGTCCAGCCTCATTTGAAAAAGGAAGAGGCGCTTTCGACAGATCAATACGTTAAAAAAGCATTGCAAAAAT
TGTTAAAGTAAGGTGATATC

Downstream 100 bases:

>100_bases
ATTTCCAAAGATTTTAATGGTGCTGGGCGCCGTCATTTTTGGCATCGGTTTATTCATGCAAGTCATCGGCAGGCTTCCCG
GCGACATTTTTGTGAAAAAG

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MDERLVSSELDNHESAIEQSLRPQRLAQYIGQEKVKDNLKVFIEAAKMREETLDHVLLYGPPGLGKTTLAAIIANEMGVN
LRTTSGPAIERPGDLAAILTALEPGDVLFIDEIHRLHRSIEEVLYPAMEDFCLDIVIGKGPSARSVRLDLPPFTLVGATT
RVGLLTAPLRDRFGVLSRLEYYTRDELSEIVIRTAELFEVDIDSLSALEIARRSRGTPRIANRLLRRVRDFAQVLGNSSI
TEEVAVDALERLQVDKLGLDHIDRKLLMGMIEKFGGGPVGIDTISATIGEESHTIEDVYEPYLLQIGFIQRTPRGRIVTP
DVYSHFKMEVPNHD

Sequences:

>Translated_334_residues
MDERLVSSELDNHESAIEQSLRPQRLAQYIGQEKVKDNLKVFIEAAKMREETLDHVLLYGPPGLGKTTLAAIIANEMGVN
LRTTSGPAIERPGDLAAILTALEPGDVLFIDEIHRLHRSIEEVLYPAMEDFCLDIVIGKGPSARSVRLDLPPFTLVGATT
RVGLLTAPLRDRFGVLSRLEYYTRDELSEIVIRTAELFEVDIDSLSALEIARRSRGTPRIANRLLRRVRDFAQVLGNSSI
TEEVAVDALERLQVDKLGLDHIDRKLLMGMIEKFGGGPVGIDTISATIGEESHTIEDVYEPYLLQIGFIQRTPRGRIVTP
DVYSHFKMEVPNHD
>Mature_334_residues
MDERLVSSELDNHESAIEQSLRPQRLAQYIGQEKVKDNLKVFIEAAKMREETLDHVLLYGPPGLGKTTLAAIIANEMGVN
LRTTSGPAIERPGDLAAILTALEPGDVLFIDEIHRLHRSIEEVLYPAMEDFCLDIVIGKGPSARSVRLDLPPFTLVGATT
RVGLLTAPLRDRFGVLSRLEYYTRDELSEIVIRTAELFEVDIDSLSALEIARRSRGTPRIANRLLRRVRDFAQVLGNSSI
TEEVAVDALERLQVDKLGLDHIDRKLLMGMIEKFGGGPVGIDTISATIGEESHTIEDVYEPYLLQIGFIQRTPRGRIVTP
DVYSHFKMEVPNHD

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family

Homologues:

Organism=Escherichia coli, GI1788167, Length=329, Percent_Identity=59.5744680851064, Blast_Score=414, Evalue=1e-117,
Organism=Escherichia coli, GI1787119, Length=138, Percent_Identity=34.0579710144928, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVB_BACLD (Q65GP6)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_080043.1
- RefSeq:   YP_092461.1
- ProteinModelPortal:   Q65GP6
- SMR:   Q65GP6
- STRING:   Q65GP6
- EnsemblBacteria:   EBBACT00000054378
- EnsemblBacteria:   EBBACT00000058825
- GeneID:   3028914
- GeneID:   3098251
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi02900
- KEGG:   bli:BL01143
- NMPDR:   fig|279010.5.peg.3227
- eggNOG:   COG2255
- GeneTree:   EBGT00050000000363
- HOGENOM:   HBG339960
- OMA:   EKFDGGP
- ProtClustDB:   PRK00080
- BioCyc:   BLIC279010-1:BLI02900-MONOMER
- BioCyc:   BLIC279010:BL01143-MONOMER
- HAMAP:   MF_00016
- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- ProDom:   PD005323
- SMART:   SM00382
- TIGRFAMs:   TIGR00635

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N

EC number: =3.6.4.12

Molecular weight: Translated: 37287; Mature: 37287

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDERLVSSELDNHESAIEQSLRPQRLAQYIGQEKVKDNLKVFIEAAKMREETLDHVLLYG
CCCHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEC
PPGLGKTTLAAIIANEMGVNLRTTSGPAIERPGDLAAILTALEPGDVLFIDEIHRLHRSI
CCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHCCCCCEEEHHHHHHHHHHH
EEVLYPAMEDFCLDIVIGKGPSARSVRLDLPPFTLVGATTRVGLLTAPLRDRFGVLSRLE
HHHHHHHHHHHEEEEEECCCCCCCEEEECCCCEEEECCHHHHHHEECCHHHHHHHHHHHH
YYTRDELSEIVIRTAELFEVDIDSLSALEIARRSRGTPRIANRLLRRVRDFAQVLGNSSI
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCC
TEEVAVDALERLQVDKLGLDHIDRKLLMGMIEKFGGGPVGIDTISATIGEESHTIEDVYE
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHH
PYLLQIGFIQRTPRGRIVTPDVYSHFKMEVPNHD
HHHHHHHHHEECCCCCEECCHHHHHEEECCCCCC
>Mature Secondary Structure
MDERLVSSELDNHESAIEQSLRPQRLAQYIGQEKVKDNLKVFIEAAKMREETLDHVLLYG
CCCHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEC
PPGLGKTTLAAIIANEMGVNLRTTSGPAIERPGDLAAILTALEPGDVLFIDEIHRLHRSI
CCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHCCCCCEEEHHHHHHHHHHH
EEVLYPAMEDFCLDIVIGKGPSARSVRLDLPPFTLVGATTRVGLLTAPLRDRFGVLSRLE
HHHHHHHHHHHEEEEEECCCCCCCEEEECCCCEEEECCHHHHHHEECCHHHHHHHHHHHH
YYTRDELSEIVIRTAELFEVDIDSLSALEIARRSRGTPRIANRLLRRVRDFAQVLGNSSI
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCC
TEEVAVDALERLQVDKLGLDHIDRKLLMGMIEKFGGGPVGIDTISATIGEESHTIEDVYE
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHH
PYLLQIGFIQRTPRGRIVTPDVYSHFKMEVPNHD
HHHHHHHHHEECCCCCEECCHHHHHEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA