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The map label for this gene is tgt [H]

Identifier: 52786629

GI number: 52786629

Start: 2787706

End: 2788851

Strand: Reverse

Name: tgt [H]

Synonym: BLi02897

Alternate gene names: 52786629

Gene position: 2788851-2787706 (Counterclockwise)

Preceding gene: 52786630

Following gene: 52786628

Centisome position: 66.05

GC content: 48.43

Gene sequence:

>1146_bases
TTGTCGCAGCAACCGATACGCTATGAGTTCATAAAAGCATGTAAACAAACAGGAGCAAGGCTGGGGAGAGTGCACACCCC
GCACGGCTCTTTTGAAACGCCGGTGTTTATGCCCGTAGGAACGCTCGCCACCGTTAAAACGATGTCGCCGGAAGAGCTGA
AAGAAATGGGTGCAGACATTATTTTAAGCAATACGTACCACCTTTGGCTGAGACCGGGCCATGACATCGTCAAAGAGGCG
GGGGGGCTTCATCAGTTTATGAATTGGAACCGCGCCATCTTGACGGATTCAGGCGGCTTTCAGGTGTTTTCCCTGAGCGA
GTTCCGCAAAATCGAAGAGGAAGGCGTTCATTTCCGCAATCATTTAAACGGAGACAAGCTGTTTTTATCTCCTGAAAAAG
CGATGGAAATCCAAAACGCCCTCGGCTCGGATATTATGATGGCGTTTGACGAATGCCCGCCATACCCGGCAGAGTACGAT
TATATGAAGAAGTCTGTTGAACGCACGAGCCGCTGGGCGGAAAGGTGCCTGAAAGCTCACAATCGCCCTGACGAGCAGGG
GCTTTTCGGAATCGTTCAAGGCGGCGAGTACGAAAATTTGAGAAAACAAAGTGCGAAAGACTTGATTTCATTGGATTTCC
CGGGATATGCTATAGGTGGACTATCCGTCGGAGAGCCAAAAGACGTGATGAACCGTGTGCTGGAGTTTACAACTCCGCTG
CTTCCAGCGGATAAACCGCGCTACTTAATGGGTGTCGGTTCACCGGACTCACTGATTGACGGAGCCATCCGCGGAGTGGA
TATGTTCGATTGCGTGCTGCCGACAAGGATAGCGAGAAACGGTACGCTCATGACGAGCGAAGGGCGCCTCGTCGTCAAAA
ATGCAAAATATGAAAGAGATTTCAGGCCGATTGATGAGCAGTGCGACTGCTATACGTGCCGCAATTACTCGCGCGCTTAT
ATCAGACATTTAATCCGCTGCAACGAAACGTTTGGAATTCGTTTAACTTCTTATCATAATCTTTATTTTCTGTTAAAATT
AATGGGGCAAGTGAGAGATGCCATTCGTGAAGATCGTTTAGGTGATTTCCGGGAAGAGTTTTTTGAGCGCTACGGTTTTA
ATAAACCAAATGCTAAAAGTTTCTGA

Upstream 100 bases:

>100_bases
TCCGCCTACCGGACCGCAGTGGAACACGAATACCGCTTCTTCAGCTTTGGAGACGCCATGTTGATCAAATAAAATTCAAA
AAGATTGGAGGGCTCATAAT

Downstream 100 bases:

>100_bases
CACATAAACGAAGAAAGCGATAAAGCCATTTGAAAGGGGTGAAAAAATAATGGAGATGCTTGGAACATTACTTCCGATTA
TTTTAATGTTTGCGGTTCTT

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 381; Mature: 380

Protein sequence:

>381_residues
MSQQPIRYEFIKACKQTGARLGRVHTPHGSFETPVFMPVGTLATVKTMSPEELKEMGADIILSNTYHLWLRPGHDIVKEA
GGLHQFMNWNRAILTDSGGFQVFSLSEFRKIEEEGVHFRNHLNGDKLFLSPEKAMEIQNALGSDIMMAFDECPPYPAEYD
YMKKSVERTSRWAERCLKAHNRPDEQGLFGIVQGGEYENLRKQSAKDLISLDFPGYAIGGLSVGEPKDVMNRVLEFTTPL
LPADKPRYLMGVGSPDSLIDGAIRGVDMFDCVLPTRIARNGTLMTSEGRLVVKNAKYERDFRPIDEQCDCYTCRNYSRAY
IRHLIRCNETFGIRLTSYHNLYFLLKLMGQVRDAIREDRLGDFREEFFERYGFNKPNAKSF

Sequences:

>Translated_381_residues
MSQQPIRYEFIKACKQTGARLGRVHTPHGSFETPVFMPVGTLATVKTMSPEELKEMGADIILSNTYHLWLRPGHDIVKEA
GGLHQFMNWNRAILTDSGGFQVFSLSEFRKIEEEGVHFRNHLNGDKLFLSPEKAMEIQNALGSDIMMAFDECPPYPAEYD
YMKKSVERTSRWAERCLKAHNRPDEQGLFGIVQGGEYENLRKQSAKDLISLDFPGYAIGGLSVGEPKDVMNRVLEFTTPL
LPADKPRYLMGVGSPDSLIDGAIRGVDMFDCVLPTRIARNGTLMTSEGRLVVKNAKYERDFRPIDEQCDCYTCRNYSRAY
IRHLIRCNETFGIRLTSYHNLYFLLKLMGQVRDAIREDRLGDFREEFFERYGFNKPNAKSF
>Mature_380_residues
SQQPIRYEFIKACKQTGARLGRVHTPHGSFETPVFMPVGTLATVKTMSPEELKEMGADIILSNTYHLWLRPGHDIVKEAG
GLHQFMNWNRAILTDSGGFQVFSLSEFRKIEEEGVHFRNHLNGDKLFLSPEKAMEIQNALGSDIMMAFDECPPYPAEYDY
MKKSVERTSRWAERCLKAHNRPDEQGLFGIVQGGEYENLRKQSAKDLISLDFPGYAIGGLSVGEPKDVMNRVLEFTTPLL
PADKPRYLMGVGSPDSLIDGAIRGVDMFDCVLPTRIARNGTLMTSEGRLVVKNAKYERDFRPIDEQCDCYTCRNYSRAYI
RHLIRCNETFGIRLTSYHNLYFLLKLMGQVRDAIREDRLGDFREEFFERYGFNKPNAKSF

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=368, Percent_Identity=44.5652173913043, Blast_Score=345, Evalue=3e-95,
Organism=Homo sapiens, GI13375872, Length=286, Percent_Identity=26.2237762237762, Blast_Score=109, Evalue=4e-24,
Organism=Escherichia coli, GI1786607, Length=359, Percent_Identity=57.6601671309192, Blast_Score=437, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI17544680, Length=354, Percent_Identity=50, Blast_Score=337, Evalue=8e-93,
Organism=Caenorhabditis elegans, GI25146502, Length=246, Percent_Identity=25.609756097561, Blast_Score=78, Evalue=8e-15,
Organism=Drosophila melanogaster, GI19920510, Length=372, Percent_Identity=45.6989247311828, Blast_Score=345, Evalue=3e-95,
Organism=Drosophila melanogaster, GI21355311, Length=255, Percent_Identity=25.0980392156863, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 43615; Mature: 43484

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQQPIRYEFIKACKQTGARLGRVHTPHGSFETPVFMPVGTLATVKTMSPEELKEMGADI
CCCCCHHHHHHHHHHHHHHHHCCEECCCCCCCCCEEEECCCHHHEECCCHHHHHHCCCCE
ILSNTYHLWLRPGHDIVKEAGGLHQFMNWNRAILTDSGGFQVFSLSEFRKIEEEGVHFRN
EEECCEEEEEECCHHHHHHCCCHHHHHCCCCEEEECCCCEEEEEHHHHHHHHHHCCHHHH
HLNGDKLFLSPEKAMEIQNALGSDIMMAFDECPPYPAEYDYMKKSVERTSRWAERCLKAH
CCCCCEEEECHHHHHHHHHHHCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
NRPDEQGLFGIVQGGEYENLRKQSAKDLISLDFPGYAIGGLSVGEPKDVMNRVLEFTTPL
CCCCCCCCEEEECCCCHHHHHHHHHHHEEEECCCCEEECCCCCCCHHHHHHHHHHHHCCC
LPADKPRYLMGVGSPDSLIDGAIRGVDMFDCVLPTRIARNGTLMTSEGRLVVKNAKYERD
CCCCCCCEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCEEECCCEEEEECCCCCCC
FRPIDEQCDCYTCRNYSRAYIRHLIRCNETFGIRLTSYHNLYFLLKLMGQVRDAIREDRL
CCCCCCCCCCHHHCCHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHCC
GDFREEFFERYGFNKPNAKSF
CHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SQQPIRYEFIKACKQTGARLGRVHTPHGSFETPVFMPVGTLATVKTMSPEELKEMGADI
CCCCHHHHHHHHHHHHHHHHCCEECCCCCCCCCEEEECCCHHHEECCCHHHHHHCCCCE
ILSNTYHLWLRPGHDIVKEAGGLHQFMNWNRAILTDSGGFQVFSLSEFRKIEEEGVHFRN
EEECCEEEEEECCHHHHHHCCCHHHHHCCCCEEEECCCCEEEEEHHHHHHHHHHCCHHHH
HLNGDKLFLSPEKAMEIQNALGSDIMMAFDECPPYPAEYDYMKKSVERTSRWAERCLKAH
CCCCCEEEECHHHHHHHHHHHCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
NRPDEQGLFGIVQGGEYENLRKQSAKDLISLDFPGYAIGGLSVGEPKDVMNRVLEFTTPL
CCCCCCCCEEEECCCCHHHHHHHHHHHEEEECCCCEEECCCCCCCHHHHHHHHHHHHCCC
LPADKPRYLMGVGSPDSLIDGAIRGVDMFDCVLPTRIARNGTLMTSEGRLVVKNAKYERD
CCCCCCCEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCEEECCCEEEEECCCCCCC
FRPIDEQCDCYTCRNYSRAYIRHLIRCNETFGIRLTSYHNLYFLLKLMGQVRDAIREDRL
CCCCCCCCCCHHHCCHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHCC
GDFREEFFERYGFNKPNAKSF
CHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA