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The map label for this gene is queA

Identifier: 52786630

GI number: 52786630

Start: 2788880

End: 2789908

Strand: Reverse

Name: queA

Synonym: BLi02898

Alternate gene names: 52786630

Gene position: 2789908-2788880 (Counterclockwise)

Preceding gene: 52786631

Following gene: 52786629

Centisome position: 66.07

GC content: 51.9

Gene sequence:

>1029_bases
ATGAAAGTAGAATTATTCGATTTTGACTTGCCTGAACGCCTCATCGCACAGGTTCCTTTAAAAGAGCGGGATGCGTCCAG
GCTGATGGTGCTGGATAAAAAAACAGGGGAGATCACTCACAGCACGTTTAAGCACGTGATTGATTTTCTGAACGCGGGCG
ATTGCATCGTTCTGAACGATACGAGGGTGCTGCCGGCCCGCTTGTACGGAGTCAAGGAAGAGACGGGAGCAAAGGTTGAA
GTTCTTCTGTTAAAACAGGAGGAAGGGGACGTCTGGGAAACGCTTGTCAAGCCCGCAAAGCGGGTCAAGCGCGGAACTGT
TCTTTCGTTCGGTGACGGCCGTCTAACGGCGGTCTGCACAGAAGAGCTCGAGCACGGCGGCAGAAAAATTGAATTCCGCT
ATGAAGGGATTTTTTACGAGGTGCTCGAGTCGCTCGGTGAAATGCCTCTGCCTCCTTATATTAAAGAACAGCTCGATGAC
AGGGAGCGCTATCAGACGGTTTACTCAAAGAAACAAGGATCGGCGGCTGCGCCGACGGCCGGGCTGCATTTTACGGAAGA
GATTCTCGATGCGCTCAGGGAAAAAGGCGTGCATATTGCGTTTATCACCCTTCATGTCGGTCTTGGGACATTCCGGCCTG
TCAGTGCGGAAAATGTAGAGGAACACGACATGCATGCCGAGTTTTATGAAATGTCGGAAGAAACGGCAGCATTGTTAAAC
CGCGTCAGACAGGAAGGCGGAAGGATCATTTCCGTCGGCACGACATCGACGAGAACGCTTGAGACAATCGCTTCCGAGCA
TGACGGCCGCTTCCGGGAAGCGAGCGGCTGGACATCGATTTTTATCTATCCCGGCTACACATTCCGGGCAATAGACGGGA
TGATTACCAATTTTCATCTTCCGAAATCGTCCCTCATTATGCTTGTCAGCGCGCTCGCAGGCAGAGAGCATGTGCTTTCC
GCCTACCGGACCGCAGTGGAACACGAATACCGCTTCTTCAGCTTTGGAGACGCCATGTTGATCAAATAA

Upstream 100 bases:

>100_bases
TGCATTGTCATCAGCATCATTCTGTCCGTCGTGTTTTCATTCTTCGGACGAATGAAATGATGCGATATTCAAGTCTATAG
ATAGAGTAAGGTGAAAAAAG

Downstream 100 bases:

>100_bases
AATTCAAAAAGATTGGAGGGCTCATAATTTGTCGCAGCAACCGATACGCTATGAGTTCATAAAAGCATGTAAACAAACAG
GAGCAAGGCTGGGGAGAGTG

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MKVELFDFDLPERLIAQVPLKERDASRLMVLDKKTGEITHSTFKHVIDFLNAGDCIVLNDTRVLPARLYGVKEETGAKVE
VLLLKQEEGDVWETLVKPAKRVKRGTVLSFGDGRLTAVCTEELEHGGRKIEFRYEGIFYEVLESLGEMPLPPYIKEQLDD
RERYQTVYSKKQGSAAAPTAGLHFTEEILDALREKGVHIAFITLHVGLGTFRPVSAENVEEHDMHAEFYEMSEETAALLN
RVRQEGGRIISVGTTSTRTLETIASEHDGRFREASGWTSIFIYPGYTFRAIDGMITNFHLPKSSLIMLVSALAGREHVLS
AYRTAVEHEYRFFSFGDAMLIK

Sequences:

>Translated_342_residues
MKVELFDFDLPERLIAQVPLKERDASRLMVLDKKTGEITHSTFKHVIDFLNAGDCIVLNDTRVLPARLYGVKEETGAKVE
VLLLKQEEGDVWETLVKPAKRVKRGTVLSFGDGRLTAVCTEELEHGGRKIEFRYEGIFYEVLESLGEMPLPPYIKEQLDD
RERYQTVYSKKQGSAAAPTAGLHFTEEILDALREKGVHIAFITLHVGLGTFRPVSAENVEEHDMHAEFYEMSEETAALLN
RVRQEGGRIISVGTTSTRTLETIASEHDGRFREASGWTSIFIYPGYTFRAIDGMITNFHLPKSSLIMLVSALAGREHVLS
AYRTAVEHEYRFFSFGDAMLIK
>Mature_342_residues
MKVELFDFDLPERLIAQVPLKERDASRLMVLDKKTGEITHSTFKHVIDFLNAGDCIVLNDTRVLPARLYGVKEETGAKVE
VLLLKQEEGDVWETLVKPAKRVKRGTVLSFGDGRLTAVCTEELEHGGRKIEFRYEGIFYEVLESLGEMPLPPYIKEQLDD
RERYQTVYSKKQGSAAAPTAGLHFTEEILDALREKGVHIAFITLHVGLGTFRPVSAENVEEHDMHAEFYEMSEETAALLN
RVRQEGGRIISVGTTSTRTLETIASEHDGRFREASGWTSIFIYPGYTFRAIDGMITNFHLPKSSLIMLVSALAGREHVLS
AYRTAVEHEYRFFSFGDAMLIK

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=345, Percent_Identity=47.2463768115942, Blast_Score=319, Evalue=2e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_BACLD (Q65GP8)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_080041.1
- RefSeq:   YP_092459.1
- ProteinModelPortal:   Q65GP8
- SMR:   Q65GP8
- STRING:   Q65GP8
- EnsemblBacteria:   EBBACT00000055066
- EnsemblBacteria:   EBBACT00000059280
- GeneID:   3028980
- GeneID:   3098241
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi02898
- KEGG:   bli:BL01141
- NMPDR:   fig|279010.5.peg.3225
- eggNOG:   COG0809
- GeneTree:   EBGT00050000002867
- HOGENOM:   HBG286076
- OMA:   SEFYRLT
- ProtClustDB:   PRK00147
- BioCyc:   BLIC279010-1:BLI02898-MONOMER
- BioCyc:   BLIC279010:BL01141-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 38662; Mature: 38662

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVELFDFDLPERLIAQVPLKERDASRLMVLDKKTGEITHSTFKHVIDFLNAGDCIVLND
CEEEEECCCCHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEC
TRVLPARLYGVKEETGAKVEVLLLKQEEGDVWETLVKPAKRVKRGTVLSFGDGRLTAVCT
CCCCHHHHCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHCCCEEEECCCCEEEEEH
EELEHGGRKIEFRYEGIFYEVLESLGEMPLPPYIKEQLDDRERYQTVYSKKQGSAAAPTA
HHHHCCCCEEEEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GLHFTEEILDALREKGVHIAFITLHVGLGTFRPVSAENVEEHDMHAEFYEMSEETAALLN
CCHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RVRQEGGRIISVGTTSTRTLETIASEHDGRFREASGWTSIFIYPGYTFRAIDGMITNFHL
HHHHCCCEEEEECCCCHHHHHHHHHHCCCCEEECCCCEEEEEECCCCEEHHHHHHHCCCC
PKSSLIMLVSALAGREHVLSAYRTAVEHEYRFFSFGDAMLIK
CHHHHHHHHHHHCCHHHHHHHHHHHHHHCCEEEEECCEEEEC
>Mature Secondary Structure
MKVELFDFDLPERLIAQVPLKERDASRLMVLDKKTGEITHSTFKHVIDFLNAGDCIVLND
CEEEEECCCCHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEC
TRVLPARLYGVKEETGAKVEVLLLKQEEGDVWETLVKPAKRVKRGTVLSFGDGRLTAVCT
CCCCHHHHCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHCCCEEEECCCCEEEEEH
EELEHGGRKIEFRYEGIFYEVLESLGEMPLPPYIKEQLDDRERYQTVYSKKQGSAAAPTA
HHHHCCCCEEEEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GLHFTEEILDALREKGVHIAFITLHVGLGTFRPVSAENVEEHDMHAEFYEMSEETAALLN
CCHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RVRQEGGRIISVGTTSTRTLETIASEHDGRFREASGWTSIFIYPGYTFRAIDGMITNFHL
HHHHCCCEEEEECCCCHHHHHHHHHHCCCCEEECCCCEEEEEECCCCEEHHHHHHHCCCC
PKSSLIMLVSALAGREHVLSAYRTAVEHEYRFFSFGDAMLIK
CHHHHHHHHHHHCCHHHHHHHHHHHHHHCCEEEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA