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The map label for this gene is hisS

Identifier: 52786615

GI number: 52786615

Start: 2771991

End: 2773265

Strand: Reverse

Name: hisS

Synonym: BLi02882

Alternate gene names: 52786615

Gene position: 2773265-2771991 (Counterclockwise)

Preceding gene: 52786616

Following gene: 52786614

Centisome position: 65.68

GC content: 48.16

Gene sequence:

>1275_bases
ATGGGATTTAATATTCCAAGGGGAACACAAGATATTCTCCCCGGAGAATCGGAACGCTGGCAATATGTCGAAAAGATTGC
GAGAGAAACGTGTCATGCGTATCAATATAAAGAAATCCGGACGCCTATTTTTGAACATACAGAACTATTTTCCCGCGGGG
TGGGAGAATCGACCGATATTGTGCAAAAAGAAATGTATACATTCGCAGACAAAAAAGGAAGAAGCCTGACGCTTCGTCCT
GAAGGCACTGCATCTGCAGTACGCTCTTTTGTGGAAAACAAACTTTTTGCAAATCCGGTTCAGCCGACGAAGCTTTACTA
CATTGGACCGATGTTCCGCTATGAAAGGCCGCAGACGGGCCGCTACCGCCAATTTTATCAATTCGGCATCGAAGCGATCG
GTTCGAACGATCCGGCGATTGACGCGGAGGTCATTGCTTTGGCGATGAGTGTATACCGGAAAGCGGGTCTCAGCAACCTG
AAGCTTGTCATTAACAGCCTCGGCGACAAAGAGAGCCGAACCGCTCACAGAGAAGCGCTGATCCGCCACTTTGAGCCGCG
GATCGACGAGTTTTGTTCAGACTGTCAAAAACGGCTCCGGCAAAACCCGCTGCGGATTCTGGACTGCAAAAAAGACAGGG
ATCATGAACTGCTGAAAACGGCTCCGTCGATTCTTGACTACTTAAATGAAGAATCTAAAGCATATTTTGCAAAGGTACAG
CAATATCTGACGGACATCGGCATTGCATTTGAGGTCGATCCGAATCTGGTGAGAGGGCTTGACTATTACAATCACACGGC
CTTTGAAATCATGAGCAATGCCGAAGGCTTCGGAGCGATTACGACGCTTGCCGGCGGGGGGCGCTATGACGGCCTTACCG
AGGAGTTCGGCGGTCCGAAAGCGCCGGGCATCGGCTTTGCGATGAGCATCGAACGCCTTCTCGCCGCACTGGATGCTGAA
AATGTAAAAGTCGGAGCGGATGAAGGCATTGACTGCTATATCGTGACACTTGGCGACAAAGCCAAAGACTATTCCGTATC
CCTTCTCTATAAACTGAGAGAAGCGGGGATATCCGCGGAAATCGACTATGAACAGAAAAAAATGAAAGGCCAGTTTAAAT
CCGCCGACCGTCTCAATGCGAAATATATCGCTGTTTTGGGCGAGGATGAACTGAACCAAAATCAAATTAATGTCAAAGAT
GGGAAAACAGGAGATCAACAGGCCATCGGCCTTGATGATTTCATCCGTTTTTTGAAAACAAACACTGAAAGTTAA

Upstream 100 bases:

>100_bases
GTATAACTCTCGTCCCTACTATTGGTTTTATAGTAGGTTCGGGGGTTTTTTTATTTCCATAAATGAGAAAAGCATTTAAG
TCATGAGGAGGATTTTAAAA

Downstream 100 bases:

>100_bases
AGGAGAGGACCGCTTTGTTTGGACGAACGTATTATTGTGGCGAAATAACGGAGAAGGCGATCGGAGAAACCGTCGTTTTA
AAAGGATGGGTTCAAAAAAG

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MGFNIPRGTQDILPGESERWQYVEKIARETCHAYQYKEIRTPIFEHTELFSRGVGESTDIVQKEMYTFADKKGRSLTLRP
EGTASAVRSFVENKLFANPVQPTKLYYIGPMFRYERPQTGRYRQFYQFGIEAIGSNDPAIDAEVIALAMSVYRKAGLSNL
KLVINSLGDKESRTAHREALIRHFEPRIDEFCSDCQKRLRQNPLRILDCKKDRDHELLKTAPSILDYLNEESKAYFAKVQ
QYLTDIGIAFEVDPNLVRGLDYYNHTAFEIMSNAEGFGAITTLAGGGRYDGLTEEFGGPKAPGIGFAMSIERLLAALDAE
NVKVGADEGIDCYIVTLGDKAKDYSVSLLYKLREAGISAEIDYEQKKMKGQFKSADRLNAKYIAVLGEDELNQNQINVKD
GKTGDQQAIGLDDFIRFLKTNTES

Sequences:

>Translated_424_residues
MGFNIPRGTQDILPGESERWQYVEKIARETCHAYQYKEIRTPIFEHTELFSRGVGESTDIVQKEMYTFADKKGRSLTLRP
EGTASAVRSFVENKLFANPVQPTKLYYIGPMFRYERPQTGRYRQFYQFGIEAIGSNDPAIDAEVIALAMSVYRKAGLSNL
KLVINSLGDKESRTAHREALIRHFEPRIDEFCSDCQKRLRQNPLRILDCKKDRDHELLKTAPSILDYLNEESKAYFAKVQ
QYLTDIGIAFEVDPNLVRGLDYYNHTAFEIMSNAEGFGAITTLAGGGRYDGLTEEFGGPKAPGIGFAMSIERLLAALDAE
NVKVGADEGIDCYIVTLGDKAKDYSVSLLYKLREAGISAEIDYEQKKMKGQFKSADRLNAKYIAVLGEDELNQNQINVKD
GKTGDQQAIGLDDFIRFLKTNTES
>Mature_423_residues
GFNIPRGTQDILPGESERWQYVEKIARETCHAYQYKEIRTPIFEHTELFSRGVGESTDIVQKEMYTFADKKGRSLTLRPE
GTASAVRSFVENKLFANPVQPTKLYYIGPMFRYERPQTGRYRQFYQFGIEAIGSNDPAIDAEVIALAMSVYRKAGLSNLK
LVINSLGDKESRTAHREALIRHFEPRIDEFCSDCQKRLRQNPLRILDCKKDRDHELLKTAPSILDYLNEESKAYFAKVQQ
YLTDIGIAFEVDPNLVRGLDYYNHTAFEIMSNAEGFGAITTLAGGGRYDGLTEEFGGPKAPGIGFAMSIERLLAALDAEN
VKVGADEGIDCYIVTLGDKAKDYSVSLLYKLREAGISAEIDYEQKKMKGQFKSADRLNAKYIAVLGEDELNQNQINVKDG
KTGDQQAIGLDDFIRFLKTNTES

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI15029520, Length=466, Percent_Identity=25.5364806866953, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI6996014, Length=468, Percent_Identity=24.7863247863248, Blast_Score=102, Evalue=6e-22,
Organism=Escherichia coli, GI1788861, Length=417, Percent_Identity=44.3645083932854, Blast_Score=360, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI71993693, Length=459, Percent_Identity=25.0544662309368, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71993686, Length=459, Percent_Identity=25.0544662309368, Blast_Score=96, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6325290, Length=482, Percent_Identity=23.8589211618257, Blast_Score=87, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24643061, Length=471, Percent_Identity=24.203821656051, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24643059, Length=487, Percent_Identity=24.0246406570842, Blast_Score=95, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_BACLD (Q65GR3)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_080026.1
- RefSeq:   YP_092444.1
- ProteinModelPortal:   Q65GR3
- SMR:   Q65GR3
- STRING:   Q65GR3
- EnsemblBacteria:   EBBACT00000057872
- EnsemblBacteria:   EBBACT00000061936
- GeneID:   3028433
- GeneID:   3098197
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi02882
- KEGG:   bli:BL01122
- NMPDR:   fig|279010.5.peg.3210
- eggNOG:   COG0124
- GeneTree:   EBGT00050000001169
- HOGENOM:   HBG616575
- OMA:   VFEWVTT
- ProtClustDB:   PRK00037
- BioCyc:   BLIC279010-1:BLI02882-MONOMER
- BioCyc:   BLIC279010:BL01122-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 47843; Mature: 47711

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFNIPRGTQDILPGESERWQYVEKIARETCHAYQYKEIRTPIFEHTELFSRGVGESTDI
CCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHH
VQKEMYTFADKKGRSLTLRPEGTASAVRSFVENKLFANPVQPTKLYYIGPMFRYERPQTG
HHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCHHCCCCCCCC
RYRQFYQFGIEAIGSNDPAIDAEVIALAMSVYRKAGLSNLKLVINSLGDKESRTAHREAL
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHH
IRHFEPRIDEFCSDCQKRLRQNPLRILDCKKDRDHELLKTAPSILDYLNEESKAYFAKVQ
HHHHCHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHH
QYLTDIGIAFEVDPNLVRGLDYYNHTAFEIMSNAEGFGAITTLAGGGRYDGLTEEFGGPK
HHHHHCCEEEEECCHHHHHHHHHCHHHHHHHHCCCCCCCCEEECCCCCCCCCHHHCCCCC
APGIGFAMSIERLLAALDAENVKVGADEGIDCYIVTLGDKAKDYSVSLLYKLREAGISAE
CCCCHHHHHHHHHHHHHCCCCEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCC
IDYEQKKMKGQFKSADRLNAKYIAVLGEDELNQNQINVKDGKTGDQQAIGLDDFIRFLKT
CCHHHHHHCCHHCCCCCCCCEEEEEECCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHC
NTES
CCCC
>Mature Secondary Structure 
GFNIPRGTQDILPGESERWQYVEKIARETCHAYQYKEIRTPIFEHTELFSRGVGESTDI
CCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHH
VQKEMYTFADKKGRSLTLRPEGTASAVRSFVENKLFANPVQPTKLYYIGPMFRYERPQTG
HHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCHHCCCCCCCC
RYRQFYQFGIEAIGSNDPAIDAEVIALAMSVYRKAGLSNLKLVINSLGDKESRTAHREAL
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHH
IRHFEPRIDEFCSDCQKRLRQNPLRILDCKKDRDHELLKTAPSILDYLNEESKAYFAKVQ
HHHHCHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHH
QYLTDIGIAFEVDPNLVRGLDYYNHTAFEIMSNAEGFGAITTLAGGGRYDGLTEEFGGPK
HHHHHCCEEEEECCHHHHHHHHHCHHHHHHHHCCCCCCCCEEECCCCCCCCCHHHCCCCC
APGIGFAMSIERLLAALDAENVKVGADEGIDCYIVTLGDKAKDYSVSLLYKLREAGISAE
CCCCHHHHHHHHHHHHHCCCCEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCC
IDYEQKKMKGQFKSADRLNAKYIAVLGEDELNQNQINVKDGKTGDQQAIGLDDFIRFLKT
CCHHHHHHCCHHCCCCCCCCEEEEEECCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHC
NTES
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA