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The map label for this gene is aspS

Identifier: 52786614

GI number: 52786614

Start: 2770198

End: 2771976

Strand: Reverse

Name: aspS

Synonym: BLi02881

Alternate gene names: 52786614

Gene position: 2771976-2770198 (Counterclockwise)

Preceding gene: 52786615

Following gene: 52786613

Centisome position: 65.65

GC content: 50.7

Gene sequence:

>1779_bases
TTGTTTGGACGAACGTATTATTGTGGCGAAATAACGGAGAAGGCGATCGGAGAAACCGTCGTTTTAAAAGGATGGGTTCA
AAAAAGACGCGATCTTGGAGGCTTGATTTTTATTGATTTGAGAGATCGTACCGGCATTGTGCAGGTCGTATTTAATCCAG
ACGTTTCCAAAGACGCCCTGGAAACAGCTGAAAGCATCCGCAGCGAATATGTGCTTGACATTACAGGCAAAGTCGTTGCC
CGTGAAGAAGCAACAGTCAATCCAAACTTGAAGACCGGAAGGATCGAAATTCAGGCGGAATCGGTAGATGTGCTGAGTGC
GGCCAAAACGCCTCCTTTTGCGATCAGCGACCAGGCTGCAGAAGTATCTGAAGACATCAGATTAAAGCACCGCTATCTCG
ACTTGCGCCGTCCGGAGATGTTCAACTCCCTGAAAATGCGCCATAACGTCACAAAAGCTGTCCGCCGCTTTTTGGATGAC
AACGGCTTTCTTGATATTGAAACGCCTGTTTTAACAAAAAGCACGCCTGAAGGCGCGCGCGACTATCTTGTACCGAGCCG
GGTGCATGAAGGGGAATTTTATGCGCTTCCGCAATCTCCGCAAATCTTTAAGCAGCTCTTGATGGTGTCAGGGTTTGACC
GCTATTATCAAATCGCCCGCTGCTTCAGGGATGAGGACCTGCGCGCCGACCGTCAGCCTGAGTTTACGCAAATCGATATC
GAAATGTCATTTATGAGCCAGGAAGACATCATGAAGCTCTCAGAAGAAATGATGGCGCATGTGATGCGTGAAACGCACGG
CATTGAAATCTCGCTTCCGCTTCCTAGAATGTCGTATGAGGATGCAATGAACCGATACGGCTCAGACAAACCGGATACGC
GCTTCGGCATGCTGTTGACGGATGTGTCAGAAGCAGTGAAGGATTCGGACTTTAAAGTGTTCGCTTCGGCTGTGGCGAAC
GGCGGAGCGGTCAAGGCGATCAATGTAAAAGGAGCCGCTGCAAACTACTCTCGCAAAGATATCGATGCGCTTGGAGAGTT
CGCTGCAAACTATGGAGCAAAAGGGCTGGCTTGGCTGAAAACAGAAGCGGATGGACTTAAAGGACCGATTGCGAAGTTTT
TTGCCGGCGAAAAACAGGAGGCACTTGTTCAGGCGCTTGACGCCGCAGACGGGGATCTGCTTCTCTTTGTGGCCGACAAG
CTCGAAGTCGCCAATGACGCGCTGGGAGCGCTTCGTTTAAAGCTTGGTAAAGAGCTGAACCTGATCGATGAAAGCTTGTT
TAACTTCTTATGGGTTGTCGACTGGCCGCTTCTTGAGTACGATGCTGAGGAAGGCCGCTACTATGCCGCACACCACCCGT
TCACAATGCCTGTCAGAGAGGATCTCAAGCTGATCGAGACGAATCCGCAGGACATGAAAGCGCAGGCTTACGACCTTGTC
CTAAACGGCTATGAACTTGGCGGCGGCTCGATCCGTATTTTTGAAAAAGACGTTCAGGAGAAAATGTTTGGCCTTCTCGG
CTTTTCAGAAGAAGAAGCGAAGGAGCAATTCGGGTTCCTGCTCGAGGCTTTTGATTACGGGGCTCCTCCGCATGGCGGAA
TTGCGCTCGGTCTTGACAGGCTGGTGATGCTGCTCAGCGGCCGTACGAATTTGAGGGATACGATCGCATTTCCGAAAACG
GCCAGCGCAAGCTGCCTGATGACCGAAGCGCCAAGCGAGGTCGACAATGCTCAGCTTGATGAGCTGCACCTTGAAATCAA
ACGCAAAATTCGACAGTAA

Upstream 100 bases:

>100_bases
ATGTCAAAGATGGGAAAACAGGAGATCAACAGGCCATCGGCCTTGATGATTTCATCCGTTTTTTGAAAACAAACACTGAA
AGTTAAAGGAGAGGACCGCT

Downstream 100 bases:

>100_bases
ATTGTAAAATATACGGCTGTACATCCCATTGAAAATCAAAGGGACGTATGATATCATTTTTAACAAATAAAGTCCTGATG
TGTTTGTTGTACACCTAGTT

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 592; Mature: 592

Protein sequence:

>592_residues
MFGRTYYCGEITEKAIGETVVLKGWVQKRRDLGGLIFIDLRDRTGIVQVVFNPDVSKDALETAESIRSEYVLDITGKVVA
REEATVNPNLKTGRIEIQAESVDVLSAAKTPPFAISDQAAEVSEDIRLKHRYLDLRRPEMFNSLKMRHNVTKAVRRFLDD
NGFLDIETPVLTKSTPEGARDYLVPSRVHEGEFYALPQSPQIFKQLLMVSGFDRYYQIARCFRDEDLRADRQPEFTQIDI
EMSFMSQEDIMKLSEEMMAHVMRETHGIEISLPLPRMSYEDAMNRYGSDKPDTRFGMLLTDVSEAVKDSDFKVFASAVAN
GGAVKAINVKGAAANYSRKDIDALGEFAANYGAKGLAWLKTEADGLKGPIAKFFAGEKQEALVQALDAADGDLLLFVADK
LEVANDALGALRLKLGKELNLIDESLFNFLWVVDWPLLEYDAEEGRYYAAHHPFTMPVREDLKLIETNPQDMKAQAYDLV
LNGYELGGGSIRIFEKDVQEKMFGLLGFSEEEAKEQFGFLLEAFDYGAPPHGGIALGLDRLVMLLSGRTNLRDTIAFPKT
ASASCLMTEAPSEVDNAQLDELHLEIKRKIRQ

Sequences:

>Translated_592_residues
MFGRTYYCGEITEKAIGETVVLKGWVQKRRDLGGLIFIDLRDRTGIVQVVFNPDVSKDALETAESIRSEYVLDITGKVVA
REEATVNPNLKTGRIEIQAESVDVLSAAKTPPFAISDQAAEVSEDIRLKHRYLDLRRPEMFNSLKMRHNVTKAVRRFLDD
NGFLDIETPVLTKSTPEGARDYLVPSRVHEGEFYALPQSPQIFKQLLMVSGFDRYYQIARCFRDEDLRADRQPEFTQIDI
EMSFMSQEDIMKLSEEMMAHVMRETHGIEISLPLPRMSYEDAMNRYGSDKPDTRFGMLLTDVSEAVKDSDFKVFASAVAN
GGAVKAINVKGAAANYSRKDIDALGEFAANYGAKGLAWLKTEADGLKGPIAKFFAGEKQEALVQALDAADGDLLLFVADK
LEVANDALGALRLKLGKELNLIDESLFNFLWVVDWPLLEYDAEEGRYYAAHHPFTMPVREDLKLIETNPQDMKAQAYDLV
LNGYELGGGSIRIFEKDVQEKMFGLLGFSEEEAKEQFGFLLEAFDYGAPPHGGIALGLDRLVMLLSGRTNLRDTIAFPKT
ASASCLMTEAPSEVDNAQLDELHLEIKRKIRQ
>Mature_592_residues
MFGRTYYCGEITEKAIGETVVLKGWVQKRRDLGGLIFIDLRDRTGIVQVVFNPDVSKDALETAESIRSEYVLDITGKVVA
REEATVNPNLKTGRIEIQAESVDVLSAAKTPPFAISDQAAEVSEDIRLKHRYLDLRRPEMFNSLKMRHNVTKAVRRFLDD
NGFLDIETPVLTKSTPEGARDYLVPSRVHEGEFYALPQSPQIFKQLLMVSGFDRYYQIARCFRDEDLRADRQPEFTQIDI
EMSFMSQEDIMKLSEEMMAHVMRETHGIEISLPLPRMSYEDAMNRYGSDKPDTRFGMLLTDVSEAVKDSDFKVFASAVAN
GGAVKAINVKGAAANYSRKDIDALGEFAANYGAKGLAWLKTEADGLKGPIAKFFAGEKQEALVQALDAADGDLLLFVADK
LEVANDALGALRLKLGKELNLIDESLFNFLWVVDWPLLEYDAEEGRYYAAHHPFTMPVREDLKLIETNPQDMKAQAYDLV
LNGYELGGGSIRIFEKDVQEKMFGLLGFSEEEAKEQFGFLLEAFDYGAPPHGGIALGLDRLVMLLSGRTNLRDTIAFPKT
ASASCLMTEAPSEVDNAQLDELHLEIKRKIRQ

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=601, Percent_Identity=40.9317803660566, Blast_Score=447, Evalue=1e-125,
Organism=Homo sapiens, GI5031815, Length=261, Percent_Identity=27.2030651340996, Blast_Score=86, Evalue=7e-17,
Organism=Homo sapiens, GI194272210, Length=261, Percent_Identity=26.8199233716475, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI45439306, Length=250, Percent_Identity=26.4, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI226491502, Length=258, Percent_Identity=25.1937984496124, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI4758762, Length=318, Percent_Identity=25.1572327044025, Blast_Score=72, Evalue=2e-12,
Organism=Escherichia coli, GI1788173, Length=592, Percent_Identity=53.3783783783784, Blast_Score=630, Evalue=0.0,
Organism=Escherichia coli, GI1789256, Length=305, Percent_Identity=25.2459016393443, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI1790571, Length=288, Percent_Identity=24.3055555555556, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1787161, Length=279, Percent_Identity=27.2401433691756, Blast_Score=77, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI32566633, Length=594, Percent_Identity=36.7003367003367, Blast_Score=340, Evalue=2e-93,
Organism=Caenorhabditis elegans, GI71994340, Length=271, Percent_Identity=24.3542435424354, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17535927, Length=271, Percent_Identity=24.3542435424354, Blast_Score=92, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17535925, Length=271, Percent_Identity=24.3542435424354, Blast_Score=92, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17551876, Length=310, Percent_Identity=26.7741935483871, Blast_Score=91, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6325153, Length=630, Percent_Identity=33.1746031746032, Blast_Score=295, Evalue=1e-80,
Organism=Saccharomyces cerevisiae, GI6321807, Length=261, Percent_Identity=25.6704980842912, Blast_Score=84, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6323011, Length=249, Percent_Identity=26.9076305220884, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320242, Length=178, Percent_Identity=26.9662921348315, Blast_Score=74, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6324256, Length=137, Percent_Identity=27.007299270073, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24584738, Length=603, Percent_Identity=39.6351575456053, Blast_Score=390, Evalue=1e-108,
Organism=Drosophila melanogaster, GI17136276, Length=241, Percent_Identity=29.4605809128631, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24640849, Length=255, Percent_Identity=27.0588235294118, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24640851, Length=257, Percent_Identity=27.2373540856031, Blast_Score=82, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19921528, Length=305, Percent_Identity=27.2131147540984, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_BACLD (Q65GR4)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_080025.1
- RefSeq:   YP_092443.1
- ProteinModelPortal:   Q65GR4
- SMR:   Q65GR4
- STRING:   Q65GR4
- EnsemblBacteria:   EBBACT00000056789
- EnsemblBacteria:   EBBACT00000061532
- GeneID:   3029050
- GeneID:   3098196
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi02881
- KEGG:   bli:BL05288
- NMPDR:   fig|279010.5.peg.3209
- eggNOG:   COG0173
- GeneTree:   EBGT00050000000858
- HOGENOM:   HBG396032
- OMA:   AFPKTQQ
- ProtClustDB:   PRK00476
- BioCyc:   BLIC279010-1:BLI02881-MONOMER
- BioCyc:   BLIC279010:BL05288-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 66378; Mature: 66378

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGRTYYCGEITEKAIGETVVLKGWVQKRRDLGGLIFIDLRDRTGIVQVVFNPDVSKDAL
CCCCEEECCHHHHHHCCCEEEHHHHHHHHHCCCCEEEEEEECCCCEEEEEECCCCCHHHH
ETAESIRSEYVLDITGKVVAREEATVNPNLKTGRIEIQAESVDVLSAAKTPPFAISDQAA
HHHHHHHHCEEEEECCCEEECCCCCCCCCCCCCEEEEEECCCHHHHHCCCCCCEECCHHH
EVSEDIRLKHRYLDLRRPEMFNSLKMRHNVTKAVRRFLDDNGFLDIETPVLTKSTPEGAR
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEECCCCCCCCH
DYLVPSRVHEGEFYALPQSPQIFKQLLMVSGFDRYYQIARCFRDEDLRADRQPEFTQIDI
HCCCCCCCCCCCEEECCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEE
EMSFMSQEDIMKLSEEMMAHVMRETHGIEISLPLPRMSYEDAMNRYGSDKPDTRFGMLLT
EEECCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHCCCCCCCCHHHHHHH
DVSEAVKDSDFKVFASAVANGGAVKAINVKGAAANYSRKDIDALGEFAANYGAKGLAWLK
HHHHHHCCCCHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEE
TEADGLKGPIAKFFAGEKQEALVQALDAADGDLLLFVADKLEVANDALGALRLKLGKELN
ECCCCCCCHHHHHHCCCHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHCCCCH
LIDESLFNFLWVVDWPLLEYDAEEGRYYAAHHPFTMPVREDLKLIETNPQDMKAQAYDLV
HHHHHHHHHHHHCCCCCEEEECCCCCEEEECCCEECCHHHCCCEECCCCHHHHHHHEEEE
LNGYELGGGSIRIFEKDVQEKMFGLLGFSEEEAKEQFGFLLEAFDYGAPPHGGIALGLDR
EECEEECCCEEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
LVMLLSGRTNLRDTIAFPKTASASCLMTEAPSEVDNAQLDELHLEIKRKIRQ
HHHHHCCCCCCCHHCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFGRTYYCGEITEKAIGETVVLKGWVQKRRDLGGLIFIDLRDRTGIVQVVFNPDVSKDAL
CCCCEEECCHHHHHHCCCEEEHHHHHHHHHCCCCEEEEEEECCCCEEEEEECCCCCHHHH
ETAESIRSEYVLDITGKVVAREEATVNPNLKTGRIEIQAESVDVLSAAKTPPFAISDQAA
HHHHHHHHCEEEEECCCEEECCCCCCCCCCCCCEEEEEECCCHHHHHCCCCCCEECCHHH
EVSEDIRLKHRYLDLRRPEMFNSLKMRHNVTKAVRRFLDDNGFLDIETPVLTKSTPEGAR
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEECCCCCCCCH
DYLVPSRVHEGEFYALPQSPQIFKQLLMVSGFDRYYQIARCFRDEDLRADRQPEFTQIDI
HCCCCCCCCCCCEEECCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEE
EMSFMSQEDIMKLSEEMMAHVMRETHGIEISLPLPRMSYEDAMNRYGSDKPDTRFGMLLT
EEECCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHCCCCCCCCHHHHHHH
DVSEAVKDSDFKVFASAVANGGAVKAINVKGAAANYSRKDIDALGEFAANYGAKGLAWLK
HHHHHHCCCCHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEE
TEADGLKGPIAKFFAGEKQEALVQALDAADGDLLLFVADKLEVANDALGALRLKLGKELN
ECCCCCCCHHHHHHCCCHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHCCCCH
LIDESLFNFLWVVDWPLLEYDAEEGRYYAAHHPFTMPVREDLKLIETNPQDMKAQAYDLV
HHHHHHHHHHHHCCCCCEEEECCCCCEEEECCCEECCHHHCCCEECCCCHHHHHHHEEEE
LNGYELGGGSIRIFEKDVQEKMFGLLGFSEEEAKEQFGFLLEAFDYGAPPHGGIALGLDR
EECEEECCCEEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
LVMLLSGRTNLRDTIAFPKTASASCLMTEAPSEVDNAQLDELHLEIKRKIRQ
HHHHHCCCCCCCHHCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA