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The map label for this gene is yrhA [H]

Identifier: 52786588

GI number: 52786588

Start: 2743980

End: 2744903

Strand: Reverse

Name: yrhA [H]

Synonym: BLi02854

Alternate gene names: 52786588

Gene position: 2744903-2743980 (Counterclockwise)

Preceding gene: 52786589

Following gene: 52786587

Centisome position: 65.0

GC content: 48.05

Gene sequence:

>924_bases
ATGAATGTGATCAAGGATATCACCGAATTGATCGGCAAAACGCCGCTTCTGCAGATTTTGGATTTTCGAATCCCTAAAGG
CGTTAACATATATGCAAAACTTGAAATGATGAATCCGGGAGGCAGCATTAAAGACCGGCTCGGAGAAGTGCTGATTGCTG
AAGCGATCGGTTCGGGAAAGCTGAAACCCGGTGGAACGGTCATTGAGGCAACAGCAGGCAATACGGGAATCGGTCTGGCT
CTCGCCGCCAGAAAGCACGGCATTCGACCCGTTTTTTGCGTACCCGAGCATTTCAGCATGGAAAAACAGTCGATTATGAA
AGCGCTCGGCGCTCGGATAGTTCATACCCCCAGAAGTGCCGGGATGAAGGGTGCGATTGAAAAAGCACTTGAACTTGAGA
AGGAGATTCCCGATTCTTATTGCGTTTTGCAGTTTAAAAACCCAGTAAATCCATTGGCTTACTATAAAACGATTGCCCCG
GAAATATGGAATGATCTTGGCGGCCGGATTGACGTCTTCGTCGCCGGAGCGGGGTCAGGCGGCACATTTGCGGGAACCGC
CAAGTTTCTGAAAGAAAAAAATCCGTCCATTAAAACGGTTATTGTTGAACCGGAAGGCTCGATTTTAAACGGAGGGGAAC
CGCATGGGCATAAGACCGAAGGAATCGGAATGGAATTCATCCCGGAATACATGGAGGAAAGCTATTTCGATGAGATTTAC
ACAGTGCTTGACGAAGATGCGTTTCGACTCGTAAAAGAAGCCGCGGAAAAGGAAGGGCTTCTGATTGGAAGCTCATCAGG
CGCCGCGTTGTTTGCCGCATTGAAAGAAGCCGAAAAAGCGAAAGCCGGCACAAACATTGTGACGATTTTTCCCGACAGCA
GCGACCGCTATTTAAGCAAAAAAATTTACGAAGGAGGAATATGA

Upstream 100 bases:

>100_bases
AAGCATTCGACAGATCTTGTGCTTCGCATGATTAAAAGAATCAATTAAAACAGGGGGGAGGCTCCCCCTCTGTTTTGCAC
CTCTTGAAGGAGGAAGGAAC

Downstream 100 bases:

>100_bases
TATGAAGCCAAAAACGAAAATGATTCACGGCGGAATTACAGGAGACGAAAAAACAGGAGCGGTATCGGTTCCTATCTATC
AGGTCAGCACGTACAAACAG

Product: YrhA

Products: L-cysteine; acetate

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MNVIKDITELIGKTPLLQILDFRIPKGVNIYAKLEMMNPGGSIKDRLGEVLIAEAIGSGKLKPGGTVIEATAGNTGIGLA
LAARKHGIRPVFCVPEHFSMEKQSIMKALGARIVHTPRSAGMKGAIEKALELEKEIPDSYCVLQFKNPVNPLAYYKTIAP
EIWNDLGGRIDVFVAGAGSGGTFAGTAKFLKEKNPSIKTVIVEPEGSILNGGEPHGHKTEGIGMEFIPEYMEESYFDEIY
TVLDEDAFRLVKEAAEKEGLLIGSSSGAALFAALKEAEKAKAGTNIVTIFPDSSDRYLSKKIYEGGI

Sequences:

>Translated_307_residues
MNVIKDITELIGKTPLLQILDFRIPKGVNIYAKLEMMNPGGSIKDRLGEVLIAEAIGSGKLKPGGTVIEATAGNTGIGLA
LAARKHGIRPVFCVPEHFSMEKQSIMKALGARIVHTPRSAGMKGAIEKALELEKEIPDSYCVLQFKNPVNPLAYYKTIAP
EIWNDLGGRIDVFVAGAGSGGTFAGTAKFLKEKNPSIKTVIVEPEGSILNGGEPHGHKTEGIGMEFIPEYMEESYFDEIY
TVLDEDAFRLVKEAAEKEGLLIGSSSGAALFAALKEAEKAKAGTNIVTIFPDSSDRYLSKKIYEGGI
>Mature_307_residues
MNVIKDITELIGKTPLLQILDFRIPKGVNIYAKLEMMNPGGSIKDRLGEVLIAEAIGSGKLKPGGTVIEATAGNTGIGLA
LAARKHGIRPVFCVPEHFSMEKQSIMKALGARIVHTPRSAGMKGAIEKALELEKEIPDSYCVLQFKNPVNPLAYYKTIAP
EIWNDLGGRIDVFVAGAGSGGTFAGTAKFLKEKNPSIKTVIVEPEGSILNGGEPHGHKTEGIGMEFIPEYMEESYFDEIY
TVLDEDAFRLVKEAAEKEGLLIGSSSGAALFAALKEAEKAKAGTNIVTIFPDSSDRYLSKKIYEGGI

Specific function: Catalyzes the conversion of O-acetylserine and homocysteine to cystathionine [H]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=309, Percent_Identity=41.1003236245955, Blast_Score=228, Evalue=6e-60,
Organism=Homo sapiens, GI295821200, Length=309, Percent_Identity=41.1003236245955, Blast_Score=228, Evalue=6e-60,
Organism=Homo sapiens, GI4557415, Length=309, Percent_Identity=41.1003236245955, Blast_Score=228, Evalue=6e-60,
Organism=Escherichia coli, GI1788754, Length=312, Percent_Identity=41.3461538461538, Blast_Score=197, Evalue=8e-52,
Organism=Escherichia coli, GI2367138, Length=303, Percent_Identity=36.6336633663366, Blast_Score=194, Evalue=6e-51,
Organism=Caenorhabditis elegans, GI17535051, Length=298, Percent_Identity=41.2751677852349, Blast_Score=224, Evalue=3e-59,
Organism=Caenorhabditis elegans, GI115535073, Length=299, Percent_Identity=40.4682274247492, Blast_Score=207, Evalue=5e-54,
Organism=Caenorhabditis elegans, GI17562970, Length=306, Percent_Identity=36.2745098039216, Blast_Score=206, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI32566674, Length=301, Percent_Identity=36.8770764119601, Blast_Score=203, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI25147552, Length=314, Percent_Identity=40.4458598726115, Blast_Score=186, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17561720, Length=306, Percent_Identity=36.6013071895425, Blast_Score=184, Evalue=4e-47,
Organism=Caenorhabditis elegans, GI17534315, Length=314, Percent_Identity=40.4458598726115, Blast_Score=184, Evalue=7e-47,
Organism=Caenorhabditis elegans, GI32566672, Length=212, Percent_Identity=35.8490566037736, Blast_Score=152, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6321594, Length=317, Percent_Identity=40.6940063091483, Blast_Score=212, Evalue=6e-56,
Organism=Saccharomyces cerevisiae, GI6321449, Length=325, Percent_Identity=36.6153846153846, Blast_Score=151, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24643623, Length=310, Percent_Identity=39.6774193548387, Blast_Score=204, Evalue=5e-53,
Organism=Drosophila melanogaster, GI20129101, Length=310, Percent_Identity=39.6774193548387, Blast_Score=204, Evalue=5e-53,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: 2.5.1.47

Molecular weight: Translated: 33014; Mature: 33014

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVIKDITELIGKTPLLQILDFRIPKGVNIYAKLEMMNPGGSIKDRLGEVLIAEAIGSGK
CCHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHCCCCC
LKPGGTVIEATAGNTGIGLALAARKHGIRPVFCVPEHFSMEKQSIMKALGARIVHTPRSA
CCCCCEEEEECCCCCCCEEEEEHHHCCCCEEEECCCCCCHHHHHHHHHHHHHEEECCHHC
GMKGAIEKALELEKEIPDSYCVLQFKNPVNPLAYYKTIAPEIWNDLGGRIDVFVAGAGSG
CHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCC
GTFAGTAKFLKEKNPSIKTVIVEPEGSILNGGEPHGHKTEGIGMEFIPEYMEESYFDEIY
CCHHHHHHHHHCCCCCEEEEEECCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
TVLDEDAFRLVKEAAEKEGLLIGSSSGAALFAALKEAEKAKAGTNIVTIFPDSSDRYLSK
HHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHH
KIYEGGI
HHHCCCC
>Mature Secondary Structure
MNVIKDITELIGKTPLLQILDFRIPKGVNIYAKLEMMNPGGSIKDRLGEVLIAEAIGSGK
CCHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHCCCCC
LKPGGTVIEATAGNTGIGLALAARKHGIRPVFCVPEHFSMEKQSIMKALGARIVHTPRSA
CCCCCEEEEECCCCCCCEEEEEHHHCCCCEEEECCCCCCHHHHHHHHHHHHHEEECCHHC
GMKGAIEKALELEKEIPDSYCVLQFKNPVNPLAYYKTIAPEIWNDLGGRIDVFVAGAGSG
CHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCC
GTFAGTAKFLKEKNPSIKTVIVEPEGSILNGGEPHGHKTEGIGMEFIPEYMEESYFDEIY
CCHHHHHHHHHCCCCCEEEEEECCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
TVLDEDAFRLVKEAAEKEGLLIGSSSGAALFAALKEAEKAKAGTNIVTIFPDSSDRYLSK
HHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHH
KIYEGGI
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 44000 [C]

Specific activity: 1100

Km value (mM): 0.12 {acetyl-CoA}} 3.6 {S2-}} 0.1 {S2-}} 20 {O-acetyl-L-Ser}} 7.1 {O-acetyl-L-Ser}} 5 {O-acetyl-L-Ser}} 0.7 {L-Ser}} [C]

Substrates: O3-acetyl-L-serine; hydrogen sulfide

Specific reaction: O3-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate

General reaction: Elimination; C-O bond cleavage [C]

Inhibitor: acetyl-CoA; D-cycloserine; L-cysteine; NH2OH; semicarbazide [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9308178; 9384377 [H]