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The map label for this gene is levE [H]

Identifier: 52786564

GI number: 52786564

Start: 2716466

End: 2716957

Strand: Reverse

Name: levE [H]

Synonym: BLi02830

Alternate gene names: 52786564

Gene position: 2716957-2716466 (Counterclockwise)

Preceding gene: 52786565

Following gene: 52786563

Centisome position: 64.34

GC content: 45.73

Gene sequence:

>492_bases
ATGAAAATCGTTTTGGCAAGAATTGATGACCGCTTTATTCACGGCCAAGTATTAACAAGATGGATCAAAATCCACGCTGC
TGATCGCATTATCGTTGTCTCCAATGAGGTGGCACAGGACGAAATGAGAAAAACGCTGATCCTTTCTGTTGCACCTTCAA
ATGTAAAAGCCAGCGCCGTTTCGATTTCCAAAATGGCCAAAGCGTTCCACAGTCCGCGGTACGAAGATACGACGGCAATG
CTGTTATTCGAAAATCCGGGCGACATTGTTTCCTTAATCGAAGCAGGCGTTCCGATCGAAATAGTCAATGTCGGCGGGAT
GCGTTTCGAGAATAACCGCAGGCAAATCACGAAATCGGTAAGCGTTACTGAAAAGGACATCAAATCGTTCGAACAATTGC
ATGAATTGGGCGTGAAGCTGGAACTGAGGCAGCTGCCGTCAGATTCGAGCGAAGACTTTATGCAGATGTTAAGAAACGCA
ACAAAATCCTAG

Upstream 100 bases:

>100_bases
TGAACCAGTTGAAAAAGATGAGCCGGGAATGCTTCCAAATATGCAGCGAGCAATTGGAAAAATTCAGTCAGTCCAATCAG
GGAAGAGAGGATGAATTGTA

Downstream 100 bases:

>100_bases
AAGGAGGTTCTAAAGGCATGTCAACGATTCAAATCATTTTGCTGCTTGTCATCGCTGCTATAACAGGTATCGGTAGTGTA
TTGGACGAAGGGCAGACACA

Product: LevE

Products: NA

Alternate protein names: EIIB-Fru; PTS system fructose-specific EIIB component; p18 [H]

Number of amino acids: Translated: 163; Mature: 163

Protein sequence:

>163_residues
MKIVLARIDDRFIHGQVLTRWIKIHAADRIIVVSNEVAQDEMRKTLILSVAPSNVKASAVSISKMAKAFHSPRYEDTTAM
LLFENPGDIVSLIEAGVPIEIVNVGGMRFENNRRQITKSVSVTEKDIKSFEQLHELGVKLELRQLPSDSSEDFMQMLRNA
TKS

Sequences:

>Translated_163_residues
MKIVLARIDDRFIHGQVLTRWIKIHAADRIIVVSNEVAQDEMRKTLILSVAPSNVKASAVSISKMAKAFHSPRYEDTTAM
LLFENPGDIVSLIEAGVPIEIVNVGGMRFENNRRQITKSVSVTEKDIKSFEQLHELGVKLELRQLPSDSSEDFMQMLRNA
TKS
>Mature_163_residues
MKIVLARIDDRFIHGQVLTRWIKIHAADRIIVVSNEVAQDEMRKTLILSVAPSNVKASAVSISKMAKAFHSPRYEDTTAM
LLFENPGDIVSLIEAGVPIEIVNVGGMRFENNRRQITKSVSVTEKDIKSFEQLHELGVKLELRQLPSDSSEDFMQMLRNA
TKS

Specific function: LevD and levE act as negative regulators of the levanase operon. They may be involved in a PTS-mediated phosphorylation of a regulator [H]

COG id: COG3444

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IIB

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI1788120, Length=157, Percent_Identity=49.0445859872611, Blast_Score=157, Evalue=3e-40,
Organism=Escherichia coli, GI87082217, Length=156, Percent_Identity=33.974358974359, Blast_Score=106, Evalue=9e-25,
Organism=Escherichia coli, GI1789527, Length=154, Percent_Identity=28.5714285714286, Blast_Score=61, Evalue=3e-11,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004720
- InterPro:   IPR018455 [H]

Pfam domain/function: PF03830 PTSIIB_sorb [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 18349; Mature: 18349

Theoretical pI: Translated: 9.05; Mature: 9.05

Prosite motif: PS51101 PTS_EIIB_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVLARIDDRFIHGQVLTRWIKIHAADRIIVVSNEVAQDEMRKTLILSVAPSNVKASAV
CEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHEEEEEECCCCCCHHHH
SISKMAKAFHSPRYEDTTAMLLFENPGDIVSLIEAGVPIEIVNVGGMRFENNRRQITKSV
HHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHCCCCEEEEECCCEEEECCHHHHHHHH
SVTEKDIKSFEQLHELGVKLELRQLPSDSSEDFMQMLRNATKS
HHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKIVLARIDDRFIHGQVLTRWIKIHAADRIIVVSNEVAQDEMRKTLILSVAPSNVKASAV
CEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHEEEEEECCCCCCHHHH
SISKMAKAFHSPRYEDTTAMLLFENPGDIVSLIEAGVPIEIVNVGGMRFENNRRQITKSV
HHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHCCCCEEEEECCCEEEECCHHHHHHHH
SVTEKDIKSFEQLHELGVKLELRQLPSDSSEDFMQMLRNATKS
HHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2117666; 9141695; 9384377; 9551099 [H]