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The map label for this gene is levD [H]

Identifier: 52786565

GI number: 52786565

Start: 2716957

End: 2717394

Strand: Reverse

Name: levD [H]

Synonym: BLi02831

Alternate gene names: 52786565

Gene position: 2717394-2716957 (Counterclockwise)

Preceding gene: 52786566

Following gene: 52786564

Centisome position: 64.35

GC content: 47.03

Gene sequence:

>438_bases
ATGATTACAGTCATTATCAGCGGCCACGGAAACTTTCCCATAGCATTAAAAGAATCTTCAGGGATGATATTCGGAGAAGA
GGAGAGCCTGATCGCGGTGCCGTTTCTGAAAGGGGAAGGCATTCAGACACTGCAAGAAAAATATCATCAGACGCTGAAGG
ATGTTCCGGAGGAACACGAGGTGCTTTTTTTAGTGGATATTTTCGGCGGGACGCCATATAACGCAGCGACCCCTTACATC
GCGAAAGAGCGGAGATTCGATATGGCAGCCGGAGTGAACCTGCCGATTTTGTTAGAGGCATTGAGCTTGAGGGAGCATAT
GCCGCTCAAACAACTGCTGAACCAGTTGAAAAAGATGAGCCGGGAATGCTTCCAAATATGCAGCGAGCAATTGGAAAAAT
TCAGTCAGTCCAATCAGGGAAGAGAGGATGAATTGTAA

Upstream 100 bases:

>100_bases
TAATTCGTCTTGAAATACAATGAAAACGCTTAATACAGTTTTGTTGGCACGATACTTGCATTATAAATAAGCGAACGAAA
AAAGAAAGGAGCAAATGTAC

Downstream 100 bases:

>100_bases
TGAAAATCGTTTTGGCAAGAATTGATGACCGCTTTATTCACGGCCAAGTATTAACAAGATGGATCAAAATCCACGCTGCT
GATCGCATTATCGTTGTCTC

Product: LevD

Products: Protein Histidine; Sugar Phosphate.; D-glucosamine-6-phosphate [Cytoplasm]; pyruvate; glucose-6-phosphate [Cytoplasm]; N-acetyl-D-glucosamine-6-phosphate [Cytoplasm]; mannose-6-phosphate [Cytoplasm]; fructose-6-phosphate [Cytoplasm] [C]

Alternate protein names: EIIA-Fru; PTS system fructose-specific EIIA component; p16 [H]

Number of amino acids: Translated: 145; Mature: 145

Protein sequence:

>145_residues
MITVIISGHGNFPIALKESSGMIFGEEESLIAVPFLKGEGIQTLQEKYHQTLKDVPEEHEVLFLVDIFGGTPYNAATPYI
AKERRFDMAAGVNLPILLEALSLREHMPLKQLLNQLKKMSRECFQICSEQLEKFSQSNQGREDEL

Sequences:

>Translated_145_residues
MITVIISGHGNFPIALKESSGMIFGEEESLIAVPFLKGEGIQTLQEKYHQTLKDVPEEHEVLFLVDIFGGTPYNAATPYI
AKERRFDMAAGVNLPILLEALSLREHMPLKQLLNQLKKMSRECFQICSEQLEKFSQSNQGREDEL
>Mature_145_residues
MITVIISGHGNFPIALKESSGMIFGEEESLIAVPFLKGEGIQTLQEKYHQTLKDVPEEHEVLFLVDIFGGTPYNAATPYI
AKERRFDMAAGVNLPILLEALSLREHMPLKQLLNQLKKMSRECFQICSEQLEKFSQSNQGREDEL

Specific function: LevD and levE act as negative regulators of the levanase operon. They may be involved in a PTS-mediated phosphorylation of a regulator [H]

COG id: COG2893

COG function: function code G; Phosphotransferase system, mannose/fructose-specific component IIA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI1788120, Length=107, Percent_Identity=37.3831775700935, Blast_Score=84, Evalue=3e-18,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004701
- InterPro:   IPR013789 [H]

Pfam domain/function: PF03610 EIIA-man [H]

EC number: 2.7.1.69 [C]

Molecular weight: Translated: 16394; Mature: 16394

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS51096 PTS_EIIA_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITVIISGHGNFPIALKESSGMIFGEEESLIAVPFLKGEGIQTLQEKYHQTLKDVPEEHE
CEEEEEECCCCCCEEEECCCCEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCC
VLFLVDIFGGTPYNAATPYIAKERRFDMAAGVNLPILLEALSLREHMPLKQLLNQLKKMS
EEEEEEECCCCCCCCCCCCHHHHHCCHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
RECFQICSEQLEKFSQSNQGREDEL
HHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MITVIISGHGNFPIALKESSGMIFGEEESLIAVPFLKGEGIQTLQEKYHQTLKDVPEEHE
CEEEEEECCCCCCEEEECCCCEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCC
VLFLVDIFGGTPYNAATPYIAKERRFDMAAGVNLPILLEALSLREHMPLKQLLNQLKKMS
EEEEEEECCCCCCCCCCCCHHHHHCCHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
RECFQICSEQLEKFSQSNQGREDEL
HHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Protein N-Phosphohistidine; Sugar; phosphoenolpyruvate; glucosamine [Periplasm]; phosphoenolpyruvate; beta-D-glucose [Periplasm]; N-acetyl-D-glucosamine [Periplasm]; mannose [Periplasm]; fructose [Periplasm] [C]

Specific reaction: Protein N-Phosphohistidine + Sugar = Protein Histidine + Sugar Phosphate. phosphoenolpyruvate + glucosamine [Periplasm] = D-glucosamine-6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + beta-D-glucose [Periplasm] = glucose-6-phosphate [Cytoplasm]

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2117666; 9141695; 9384377 [H]