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The map label for this gene is levF [H]

Identifier: 52786563

GI number: 52786563

Start: 2715645

End: 2716448

Strand: Reverse

Name: levF [H]

Synonym: BLi02829

Alternate gene names: 52786563

Gene position: 2716448-2715645 (Counterclockwise)

Preceding gene: 52786564

Following gene: 52786562

Centisome position: 64.33

GC content: 50.5

Gene sequence:

>804_bases
ATGTCAACGATTCAAATCATTTTGCTGCTTGTCATCGCTGCTATAACAGGTATCGGTAGTGTATTGGACGAAGGGCAGAC
ACATCGCCCGCTGATTGCCTGCACTTTGGTCGGCCTTGTCCTCGGGGATCTGAAAACAGGCATCATTCTCGGCGGAACGC
TGGAGCTGATGGCGCTTGGCTGGATGAATGTCGGTCTTGCAATGGCCCCCGATACGGCCATCGCTTCCGTGATTTCGACT
ATCTTAGTGATTACCGCCGATCAGGGAATCGGCGAAGGGATCGCCGTCGCGGTGGCTTTGGCAGCTGCGGGTCAGGCGCT
GACGATTTTCGTCCGGACGATCACCGTATTTTTAATTCACCGCGCCGACAAATACGCAAAGGATGGCAATTTTAAAGGAA
TTGAAATTATGCATATAACTGCCATGGCGTTTCAGGCGCTGCGCGTTATGATTCCGACATTGATCGTGGCTCTGATCAGT
GTCAGCGCTGTTCAGGCGTTTTTGGGAAATATTCCGGAAGTCATCACAAAAGGCTTGCAAATAGGCGGAGGCATTATCGT
TGTTGTCGGGTATGCGATGGTCATCAACATGATGAACATTCCGTATTTGAAGCCGTTTTTCTATATCGGTTTTTTGCTGG
CCGCGTTCACCGATTTCAATTTAGTCGGCTTCGGCGCCCTCGGGCTCTGTCTGGCATTTTTATATCAGCAAGTGATGCAA
AAAAATCATGCTCAAGGGGCGGTTGCTGCCGCCAGTGACGGCAGCATGGCCGCTTATGATGATGACGATGATCTGGATGC
TTAA

Upstream 100 bases:

>100_bases
GCGTGAAGCTGGAACTGAGGCAGCTGCCGTCAGATTCGAGCGAAGACTTTATGCAGATGTTAAGAAACGCAACAAAATCC
TAGAAGGAGGTTCTAAAGGC

Downstream 100 bases:

>100_bases
AAATTAAAGGAGGGACGAATAATGGGCACAGAAAAAAGATTAACGAAGAGAGAGATTTTCAGTATGTTTATCCGCTCAAA
CTTTTTGCTCGGATCTTTTA

Product: LevF

Products: protein histidine; sugar phosphate; D-glucosamine-6-phosphate [Cytoplasm]; pyruvate; glucose-6-phosphate [Cytoplasm]; N-acetyl-D-glucosamine-6-phosphate [Cytoplasm]; mannose-6-phosphate [Cytoplasm]; fructose-6-phosphate [Cytoplasm] [C]

Alternate protein names: EIIC-Fru; PTS system fructose-specific EIIC component; p28 [H]

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MSTIQIILLLVIAAITGIGSVLDEGQTHRPLIACTLVGLVLGDLKTGIILGGTLELMALGWMNVGLAMAPDTAIASVIST
ILVITADQGIGEGIAVAVALAAAGQALTIFVRTITVFLIHRADKYAKDGNFKGIEIMHITAMAFQALRVMIPTLIVALIS
VSAVQAFLGNIPEVITKGLQIGGGIIVVVGYAMVINMMNIPYLKPFFYIGFLLAAFTDFNLVGFGALGLCLAFLYQQVMQ
KNHAQGAVAAASDGSMAAYDDDDDLDA

Sequences:

>Translated_267_residues
MSTIQIILLLVIAAITGIGSVLDEGQTHRPLIACTLVGLVLGDLKTGIILGGTLELMALGWMNVGLAMAPDTAIASVIST
ILVITADQGIGEGIAVAVALAAAGQALTIFVRTITVFLIHRADKYAKDGNFKGIEIMHITAMAFQALRVMIPTLIVALIS
VSAVQAFLGNIPEVITKGLQIGGGIIVVVGYAMVINMMNIPYLKPFFYIGFLLAAFTDFNLVGFGALGLCLAFLYQQVMQ
KNHAQGAVAAASDGSMAAYDDDDDLDA
>Mature_266_residues
STIQIILLLVIAAITGIGSVLDEGQTHRPLIACTLVGLVLGDLKTGIILGGTLELMALGWMNVGLAMAPDTAIASVISTI
LVITADQGIGEGIAVAVALAAAGQALTIFVRTITVFLIHRADKYAKDGNFKGIEIMHITAMAFQALRVMIPTLIVALISV
SAVQAFLGNIPEVITKGLQIGGGIIVVVGYAMVINMMNIPYLKPFFYIGFLLAAFTDFNLVGFGALGLCLAFLYQQVMQK
NHAQGAVAAASDGSMAAYDDDDDLDA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG3715

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IIC

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI1788121, Length=268, Percent_Identity=59.3283582089552, Blast_Score=273, Evalue=1e-74,
Organism=Escherichia coli, GI1789528, Length=255, Percent_Identity=25.8823529411765, Blast_Score=89, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004700
- InterPro:   IPR018404 [H]

Pfam domain/function: PF03609 EII-Sor [H]

EC number: NA

Molecular weight: Translated: 27913; Mature: 27781

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS51106 PTS_EIIC_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTIQIILLLVIAAITGIGSVLDEGQTHRPLIACTLVGLVLGDLKTGIILGGTLELMALG
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCEEECCHHHHHHHH
WMNVGLAMAPDTAIASVISTILVITADQGIGEGIAVAVALAAAGQALTIFVRTITVFLIH
HHHCCCEECCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHH
RADKYAKDGNFKGIEIMHITAMAFQALRVMIPTLIVALISVSAVQAFLGNIPEVITKGLQ
HHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
IGGGIIVVVGYAMVINMMNIPYLKPFFYIGFLLAAFTDFNLVGFGALGLCLAFLYQQVMQ
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
KNHAQGAVAAASDGSMAAYDDDDDLDA
HCCCCCCEEECCCCCEECCCCCCCCCC
>Mature Secondary Structure 
STIQIILLLVIAAITGIGSVLDEGQTHRPLIACTLVGLVLGDLKTGIILGGTLELMALG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCEEECCHHHHHHHH
WMNVGLAMAPDTAIASVISTILVITADQGIGEGIAVAVALAAAGQALTIFVRTITVFLIH
HHHCCCEECCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHH
RADKYAKDGNFKGIEIMHITAMAFQALRVMIPTLIVALISVSAVQAFLGNIPEVITKGLQ
HHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
IGGGIIVVVGYAMVINMMNIPYLKPFFYIGFLLAAFTDFNLVGFGALGLCLAFLYQQVMQ
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
KNHAQGAVAAASDGSMAAYDDDDDLDA
HCCCCCCEEECCCCCEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N p -phosphohistidine; sugar; phosphoenolpyruvate; glucosamine [Periplasm]; phosphoenolpyruvate; beta-D-glucose [Periplasm]; N-acetyl-D-glucosamine [Periplasm]; mannose [Periplasm]; fructose [Periplasm] [C]

Specific reaction: protein N p -phosphohistidine + sugar = protein histidine + sugar phosphate phosphoenolpyruvate + glucosamine [Periplasm] = D-glucosamine-6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + beta-D-glucose [Periplasm] = glucose-6-phosphate [Cytoplasm]

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2117666; 9141695; 9384377 [H]