Click here to switch to the map view.

The map label for this gene is levG [H]

Identifier: 52786562

GI number: 52786562

Start: 2714796

End: 2715623

Strand: Reverse

Name: levG [H]

Synonym: BLi02828

Alternate gene names: 52786562

Gene position: 2715623-2714796 (Counterclockwise)

Preceding gene: 52786563

Following gene: 52786561

Centisome position: 64.31

GC content: 47.95

Gene sequence:

>828_bases
ATGGGCACAGAAAAAAGATTAACGAAGAGAGAGATTTTCAGTATGTTTATCCGCTCAAACTTTTTGCTCGGATCTTTTAA
CTTTGAGCGTGTACAGGCGCTCGGCTACTGCTACGTCATGATTCCGGCGATCAAAAAACTGTACGGTCCGGGAGCAAAAC
GGAGCGAAGCGCTCCAGCGCCATTTGGAATGGTTTAATACACATCCGTGGCTGACAGCGCCGATATTTGGCGTAACCGCT
GCGATGGAAGAAGAAATGGCCAACAATAAAAAGATTGATGCCAAAGCAATTAATGGAATGAAAATCGGCTTAATGGGCCC
GTTGGCAGGCGTGGGCGACCCGATTTTCTGGGGGACGTTGCGCCCGGTTTTGGCGGCCTTGGGAGCGTCGCTTGCTTTAA
GCGGAAACATCGCCGGCCCGCTTTTGTTTTTCTTTTTAATCAACGCCGTCAGATTAAGCACTAAATACTACGGATTAAAG
TACGGCTATGTGAAAGGCATGGAAATATTGCAGGACTTGGTGGGGAACCGCATTCAAAAACTGACGGAAGGCGCTTCAAT
ACTCGGGCTGTTTGTGATGGGAGCTCTCGTTTCCAAGTGGACCACAATCAATATCCCGATCGTCGTGTCGAGAATTAAGG
ATGATGCAGGCAAAGTCGATGTACAGACCGTGCAAAACATTTTAGACAGCATCATGCCTGGTGTGCTGCCTTTAGGATTG
ACATTGCTCGCTGCCTGGATGCTTCGCAAAGGGGTGAATCCTCTGCTCATGATTTTCGGTATTTTTATCATCGGGATTGT
CGGCTATTGGGCAGGTTTTTTGGCATAA

Upstream 100 bases:

>100_bases
TCATGCTCAAGGGGCGGTTGCTGCCGCCAGTGACGGCAGCATGGCCGCTTATGATGATGACGATGATCTGGATGCTTAAA
AATTAAAGGAGGGACGAATA

Downstream 100 bases:

>100_bases
CATGTTTTGGGTGTTCTTTTCTTAGCTTTTGAGAAGCAGGTCCATTCGGCCGCAAAAAAGGGGGCGGCTGTTTGGACTTC
CTGCCTAAAAAACCGAGTGA

Product: LevG

Products: protein histidine; sugar phosphate; D-glucosamine-6-phosphate [Cytoplasm]; pyruvate; glucose-6-phosphate [Cytoplasm]; N-acetyl-D-glucosamine-6-phosphate [Cytoplasm]; mannose-6-phosphate [Cytoplasm]; fructose-6-phosphate [Cytoplasm] [C]

Alternate protein names: EIID-Fru; PTS system fructose-specific EIID component; p30 [H]

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MGTEKRLTKREIFSMFIRSNFLLGSFNFERVQALGYCYVMIPAIKKLYGPGAKRSEALQRHLEWFNTHPWLTAPIFGVTA
AMEEEMANNKKIDAKAINGMKIGLMGPLAGVGDPIFWGTLRPVLAALGASLALSGNIAGPLLFFFLINAVRLSTKYYGLK
YGYVKGMEILQDLVGNRIQKLTEGASILGLFVMGALVSKWTTINIPIVVSRIKDDAGKVDVQTVQNILDSIMPGVLPLGL
TLLAAWMLRKGVNPLLMIFGIFIIGIVGYWAGFLA

Sequences:

>Translated_275_residues
MGTEKRLTKREIFSMFIRSNFLLGSFNFERVQALGYCYVMIPAIKKLYGPGAKRSEALQRHLEWFNTHPWLTAPIFGVTA
AMEEEMANNKKIDAKAINGMKIGLMGPLAGVGDPIFWGTLRPVLAALGASLALSGNIAGPLLFFFLINAVRLSTKYYGLK
YGYVKGMEILQDLVGNRIQKLTEGASILGLFVMGALVSKWTTINIPIVVSRIKDDAGKVDVQTVQNILDSIMPGVLPLGL
TLLAAWMLRKGVNPLLMIFGIFIIGIVGYWAGFLA
>Mature_274_residues
GTEKRLTKREIFSMFIRSNFLLGSFNFERVQALGYCYVMIPAIKKLYGPGAKRSEALQRHLEWFNTHPWLTAPIFGVTAA
MEEEMANNKKIDAKAINGMKIGLMGPLAGVGDPIFWGTLRPVLAALGASLALSGNIAGPLLFFFLINAVRLSTKYYGLKY
GYVKGMEILQDLVGNRIQKLTEGASILGLFVMGALVSKWTTINIPIVVSRIKDDAGKVDVQTVQNILDSIMPGVLPLGLT
LLAAWMLRKGVNPLLMIFGIFIIGIVGYWAGFLA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG3716

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IID

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIID domain [H]

Homologues:

Organism=Escherichia coli, GI1788122, Length=273, Percent_Identity=62.6373626373626, Blast_Score=357, Evalue=1e-100,
Organism=Escherichia coli, GI1789529, Length=281, Percent_Identity=37.7224199288256, Blast_Score=162, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004704
- InterPro:   IPR018405 [H]

Pfam domain/function: PF03613 EIID-AGA [H]

EC number: NA

Molecular weight: Translated: 30174; Mature: 30042

Theoretical pI: Translated: 10.30; Mature: 10.30

Prosite motif: PS51108 PTS_EIID

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTEKRLTKREIFSMFIRSNFLLGSFNFERVQALGYCYVMIPAIKKLYGPGAKRSEALQR
CCCHHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
HLEWFNTHPWLTAPIFGVTAAMEEEMANNKKIDAKAINGMKIGLMGPLAGVGDPIFWGTL
HHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCEEEEECCCCCCCCCCHHHHHH
RPVLAALGASLALSGNIAGPLLFFFLINAVRLSTKYYGLKYGYVKGMEILQDLVGNRIQK
HHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
LTEGASILGLFVMGALVSKWTTINIPIVVSRIKDDAGKVDVQTVQNILDSIMPGVLPLGL
HHHHHHHHHHHHHHHHHHHCCEECHHHHEEEHHCCCCCEEHHHHHHHHHHHHHHHHHHHH
TLLAAWMLRKGVNPLLMIFGIFIIGIVGYWAGFLA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GTEKRLTKREIFSMFIRSNFLLGSFNFERVQALGYCYVMIPAIKKLYGPGAKRSEALQR
CCHHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
HLEWFNTHPWLTAPIFGVTAAMEEEMANNKKIDAKAINGMKIGLMGPLAGVGDPIFWGTL
HHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCEEEEECCCCCCCCCCHHHHHH
RPVLAALGASLALSGNIAGPLLFFFLINAVRLSTKYYGLKYGYVKGMEILQDLVGNRIQK
HHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
LTEGASILGLFVMGALVSKWTTINIPIVVSRIKDDAGKVDVQTVQNILDSIMPGVLPLGL
HHHHHHHHHHHHHHHHHHHCCEECHHHHEEEHHCCCCCEEHHHHHHHHHHHHHHHHHHHH
TLLAAWMLRKGVNPLLMIFGIFIIGIVGYWAGFLA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N p -phosphohistidine; sugar; phosphoenolpyruvate; glucosamine [Periplasm]; phosphoenolpyruvate; beta-D-glucose [Periplasm]; N-acetyl-D-glucosamine [Periplasm]; mannose [Periplasm]; fructose [Periplasm] [C]

Specific reaction: protein N p -phosphohistidine + sugar = protein histidine + sugar phosphate phosphoenolpyruvate + glucosamine [Periplasm] = D-glucosamine-6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + beta-D-glucose [Periplasm] = glucose-6-phosphate [Cytoplasm]

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2117666; 9141695; 9384377 [H]