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The map label for this gene is phoB [H]

Identifier: 52786303

GI number: 52786303

Start: 2482956

End: 2484617

Strand: Reverse

Name: phoB [H]

Synonym: BLi02565

Alternate gene names: 52786303

Gene position: 2484617-2482956 (Counterclockwise)

Preceding gene: 52786304

Following gene: 52786302

Centisome position: 58.84

GC content: 47.35

Gene sequence:

>1662_bases
ATGGGTTTCTTACGCAACAGAATCGTCGGCATTACACTGGCAGGCGCAGTCGCTTTAGGATCGGCGGGAACAGGCAGTGC
GGCAATGGAAACAGCGAAAAAAAAAGAAGAGCCGAAGGTCAAAAACGTGATCATGATGGTCATGGATGGGACAAGCTCTT
CTGCAACAACTTTGGCACGCTGGTATAAAGGCGAACCGCTCGCAATGGACCAGATCGTCAGCGGCGGTGTCCGGACGTAT
TCGGCAGAATCGGCGATCACTGATTCTGCACCTGCAGCAACTGCGATGGCAACGGGACGAAAATCAAATTCTTCCTATAT
CGGTGTGCTGCCTTCAAAAGTGAATACACCAGGAGTCGAGAAGGTCCGTGAAAAAGATCATTACCGGCCTGTAGCAAACG
TGCTGGAAGGCGCAAAACAGAAGGGGAAAGCAACCGGCATCATTGCAACATCTGAAATTCAGCATGCCACGCCTGCCGGC
TATTCCGGCCATCATAAAGACCGCAATAATTTTGAAGTGCTCGGTGAACAGCAGGTTTATCAAAACATGGATGTTGTCCT
TGGCGGAGGAAAAGAATCGCTTCAGCCGGGTACAAAGGATAAAAGCCGCAAAGACGGTGAAGACCTTGTAAAAGTGCTCA
AAGATAAAAAGTACGATTTCGTTGAAACGAAAGATCAACTCCTCAAGTCAAAATCGAAAAAAATCTGGGGCGCCTTCTCA
CAGCGCGATCTCGCGTATGACATGGACCGCCGCGCAACGCATCCAGAGCAGCCGACTATTGCAGAGATGACGAAAAAATC
GATTCAGACGCTTTCTAAAGACAAGGACGGTTTCTTTTTATTCGTGGAAGGAAGCAAGCCTGACTGGGCAGCGCATCAAA
ACGATCCGATCGGCATGATCAGCGATGTATTGGCGTTTGATAACGCGGTGAAAGAAGCGCTTGATTTCGCTAAAAAAGAT
AAACATACAATGGTTATTGCCGTCTCAGACCATGGCAACAGCGGCATCTCCATCGGCAACAGCAACACGACGAAAGGCTA
TGACACAACACCGGTATCAGCCTATGTCGATCCGCTGAAAAAAGCTAAAATGACGGTGGAAGGCGCAACTGGCAAATTAA
AAGACGACTTATCGAATATCGAAGAAGCGGCGAAGCTTTACGGACTTGATGATTTAACGCCGGCAGAGAAGGAACAGCTG
AAAAGCGTGAAAAACAAAAAAGAAGCGGCTTCATTATTTACCAAGCTGCTCAGCAACCGGGCCAACATCGGATTCACAAC
AGGCGGTCATACGGGTGAAGACGTCTTCCTGTATTCGTATGGTCCAGAAAAGCCGCAGGGTCTCGTCGAAAACACCGATA
TCGCCAAAACGATGGCAAAGGCAATGGGCTTCAACTTAGACAAATTAACAAACGAATTATTTATCGAAGCAAAACAGGCA
TTTAAAAAAGCCGGAGCCAGCGTAACGATCGATCAAAAAGATCCGGCTAACCCAGTTCTTGTCGCAGCGCGCAAAAAAGC
AAAAGCCGAAATGCCTGTGAACAAAAACATTATCAGAATCAACGGGAAGGAATACGAACTAGACAGCGTGATTGTTGAAA
GCAATGGATCCTTCTACGTGCCTAAAGAAGCAGTTCAGCTATTCAAAAAATACGCCAGATAA

Upstream 100 bases:

>100_bases
AAAATTTACATAAGATTAATAGAAAGCAAAAGTGCTGTTTACATAGCAATGATAGTGTAGGTGTGTCAACAAAATGCAGT
TATGGAGGGAGTACATAAGA

Downstream 100 bases:

>100_bases
TTTATTTGAAAACCCCTCTGGAAAGAAGAGGGGTTTTTTTATTGTTCAAAATAAATACATACATTTATTTTTAAATGTCT
AGACATTTAAAAATAAAAAG

Product: PhoB

Products: NA

Alternate protein names: Alkaline phosphatase III; APase III [H]

Number of amino acids: Translated: 553; Mature: 552

Protein sequence:

>553_residues
MGFLRNRIVGITLAGAVALGSAGTGSAAMETAKKKEEPKVKNVIMMVMDGTSSSATTLARWYKGEPLAMDQIVSGGVRTY
SAESAITDSAPAATAMATGRKSNSSYIGVLPSKVNTPGVEKVREKDHYRPVANVLEGAKQKGKATGIIATSEIQHATPAG
YSGHHKDRNNFEVLGEQQVYQNMDVVLGGGKESLQPGTKDKSRKDGEDLVKVLKDKKYDFVETKDQLLKSKSKKIWGAFS
QRDLAYDMDRRATHPEQPTIAEMTKKSIQTLSKDKDGFFLFVEGSKPDWAAHQNDPIGMISDVLAFDNAVKEALDFAKKD
KHTMVIAVSDHGNSGISIGNSNTTKGYDTTPVSAYVDPLKKAKMTVEGATGKLKDDLSNIEEAAKLYGLDDLTPAEKEQL
KSVKNKKEAASLFTKLLSNRANIGFTTGGHTGEDVFLYSYGPEKPQGLVENTDIAKTMAKAMGFNLDKLTNELFIEAKQA
FKKAGASVTIDQKDPANPVLVAARKKAKAEMPVNKNIIRINGKEYELDSVIVESNGSFYVPKEAVQLFKKYAR

Sequences:

>Translated_553_residues
MGFLRNRIVGITLAGAVALGSAGTGSAAMETAKKKEEPKVKNVIMMVMDGTSSSATTLARWYKGEPLAMDQIVSGGVRTY
SAESAITDSAPAATAMATGRKSNSSYIGVLPSKVNTPGVEKVREKDHYRPVANVLEGAKQKGKATGIIATSEIQHATPAG
YSGHHKDRNNFEVLGEQQVYQNMDVVLGGGKESLQPGTKDKSRKDGEDLVKVLKDKKYDFVETKDQLLKSKSKKIWGAFS
QRDLAYDMDRRATHPEQPTIAEMTKKSIQTLSKDKDGFFLFVEGSKPDWAAHQNDPIGMISDVLAFDNAVKEALDFAKKD
KHTMVIAVSDHGNSGISIGNSNTTKGYDTTPVSAYVDPLKKAKMTVEGATGKLKDDLSNIEEAAKLYGLDDLTPAEKEQL
KSVKNKKEAASLFTKLLSNRANIGFTTGGHTGEDVFLYSYGPEKPQGLVENTDIAKTMAKAMGFNLDKLTNELFIEAKQA
FKKAGASVTIDQKDPANPVLVAARKKAKAEMPVNKNIIRINGKEYELDSVIVESNGSFYVPKEAVQLFKKYAR
>Mature_552_residues
GFLRNRIVGITLAGAVALGSAGTGSAAMETAKKKEEPKVKNVIMMVMDGTSSSATTLARWYKGEPLAMDQIVSGGVRTYS
AESAITDSAPAATAMATGRKSNSSYIGVLPSKVNTPGVEKVREKDHYRPVANVLEGAKQKGKATGIIATSEIQHATPAGY
SGHHKDRNNFEVLGEQQVYQNMDVVLGGGKESLQPGTKDKSRKDGEDLVKVLKDKKYDFVETKDQLLKSKSKKIWGAFSQ
RDLAYDMDRRATHPEQPTIAEMTKKSIQTLSKDKDGFFLFVEGSKPDWAAHQNDPIGMISDVLAFDNAVKEALDFAKKDK
HTMVIAVSDHGNSGISIGNSNTTKGYDTTPVSAYVDPLKKAKMTVEGATGKLKDDLSNIEEAAKLYGLDDLTPAEKEQLK
SVKNKKEAASLFTKLLSNRANIGFTTGGHTGEDVFLYSYGPEKPQGLVENTDIAKTMAKAMGFNLDKLTNELFIEAKQAF
KKAGASVTIDQKDPANPVLVAARKKAKAEMPVNKNIIRINGKEYELDSVIVESNGSFYVPKEAVQLFKKYAR

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI157266296, Length=478, Percent_Identity=28.8702928870293, Blast_Score=129, Evalue=5e-30,
Organism=Homo sapiens, GI94721246, Length=478, Percent_Identity=27.8242677824268, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI157266292, Length=351, Percent_Identity=29.0598290598291, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI116734717, Length=339, Percent_Identity=28.3185840707965, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI294660770, Length=335, Percent_Identity=27.4626865671642, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI294660772, Length=291, Percent_Identity=27.4914089347079, Blast_Score=104, Evalue=2e-22,
Organism=Escherichia coli, GI48994877, Length=347, Percent_Identity=30.835734870317, Blast_Score=124, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6320689, Length=522, Percent_Identity=30.6513409961686, Blast_Score=186, Evalue=8e-48,
Organism=Drosophila melanogaster, GI85725200, Length=342, Percent_Identity=29.5321637426901, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI85815833, Length=342, Percent_Identity=29.5321637426901, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI19921912, Length=480, Percent_Identity=27.2916666666667, Blast_Score=128, Evalue=9e-30,
Organism=Drosophila melanogaster, GI24657827, Length=492, Percent_Identity=29.0650406504065, Blast_Score=128, Evalue=1e-29,
Organism=Drosophila melanogaster, GI21355151, Length=458, Percent_Identity=27.7292576419214, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI21355981, Length=465, Percent_Identity=25.8064516129032, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24657835, Length=482, Percent_Identity=27.5933609958506, Blast_Score=117, Evalue=3e-26,
Organism=Drosophila melanogaster, GI21355149, Length=342, Percent_Identity=29.2397660818713, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24585213, Length=393, Percent_Identity=29.0076335877863, Blast_Score=113, Evalue=4e-25,
Organism=Drosophila melanogaster, GI18859923, Length=447, Percent_Identity=24.8322147651007, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24651554, Length=485, Percent_Identity=25.360824742268, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI21358067, Length=477, Percent_Identity=26.2054507337526, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24657842, Length=490, Percent_Identity=25.5102040816327, Blast_Score=107, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24651556, Length=470, Percent_Identity=25.1063829787234, Blast_Score=99, Evalue=7e-21,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 60020; Mature: 59889

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFLRNRIVGITLAGAVALGSAGTGSAAMETAKKKEEPKVKNVIMMVMDGTSSSATTLAR
CCCHHHHEEEEEEEHHHHCCCCCCCHHHHHHHHHHCCCCHHEEEEEEECCCCCCHHHHHH
WYKGEPLAMDQIVSGGVRTYSAESAITDSAPAATAMATGRKSNSSYIGVLPSKVNTPGVE
HHCCCCCCHHHHHCCCCEEECCCCCCCCCCCCHHHHHCCCCCCCCEEEEECCCCCCCCHH
KVREKDHYRPVANVLEGAKQKGKATGIIATSEIQHATPAGYSGHHKDRNNFEVLGEQQVY
HHHHHHCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHEECHHHHH
QNMDVVLGGGKESLQPGTKDKSRKDGEDLVKVLKDKKYDFVETKDQLLKSKSKKIWGAFS
CCCCEEEECCHHHCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
QRDLAYDMDRRATHPEQPTIAEMTKKSIQTLSKDKDGFFLFVEGSKPDWAAHQNDPIGMI
CCCHHHCCHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHH
SDVLAFDNAVKEALDFAKKDKHTMVIAVSDHGNSGISIGNSNTTKGYDTTPVSAYVDPLK
HHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEECCCCCCCCCCCCCHHHHHHHHH
KAKMTVEGATGKLKDDLSNIEEAAKLYGLDDLTPAEKEQLKSVKNKKEAASLFTKLLSNR
HHEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
ANIGFTTGGHTGEDVFLYSYGPEKPQGLVENTDIAKTMAKAMGFNLDKLTNELFIEAKQA
CCCCEECCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
FKKAGASVTIDQKDPANPVLVAARKKAKAEMPVNKNIIRINGKEYELDSVIVESNGSFYV
HHHCCCEEEECCCCCCCCEEEEEHHHCCCCCCCCCCEEEECCCEEEECEEEEECCCCEEC
PKEAVQLFKKYAR
CHHHHHHHHHHCC
>Mature Secondary Structure 
GFLRNRIVGITLAGAVALGSAGTGSAAMETAKKKEEPKVKNVIMMVMDGTSSSATTLAR
CCHHHHEEEEEEEHHHHCCCCCCCHHHHHHHHHHCCCCHHEEEEEEECCCCCCHHHHHH
WYKGEPLAMDQIVSGGVRTYSAESAITDSAPAATAMATGRKSNSSYIGVLPSKVNTPGVE
HHCCCCCCHHHHHCCCCEEECCCCCCCCCCCCHHHHHCCCCCCCCEEEEECCCCCCCCHH
KVREKDHYRPVANVLEGAKQKGKATGIIATSEIQHATPAGYSGHHKDRNNFEVLGEQQVY
HHHHHHCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHEECHHHHH
QNMDVVLGGGKESLQPGTKDKSRKDGEDLVKVLKDKKYDFVETKDQLLKSKSKKIWGAFS
CCCCEEEECCHHHCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
QRDLAYDMDRRATHPEQPTIAEMTKKSIQTLSKDKDGFFLFVEGSKPDWAAHQNDPIGMI
CCCHHHCCHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHH
SDVLAFDNAVKEALDFAKKDKHTMVIAVSDHGNSGISIGNSNTTKGYDTTPVSAYVDPLK
HHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEECCCCCCCCCCCCCHHHHHHHHH
KAKMTVEGATGKLKDDLSNIEEAAKLYGLDDLTPAEKEQLKSVKNKKEAASLFTKLLSNR
HHEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
ANIGFTTGGHTGEDVFLYSYGPEKPQGLVENTDIAKTMAKAMGFNLDKLTNELFIEAKQA
CCCCEECCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
FKKAGASVTIDQKDPANPVLVAARKKAKAEMPVNKNIIRINGKEYELDSVIVESNGSFYV
HHHCCCEEEECCCCCCCCEEEEEHHHCCCCCCCCCCEEEECCCEEEECEEEEECCCCEEC
PKEAVQLFKKYAR
CHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1898729; 9202461; 9384377; 2113910; 2105301 [H]