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The map label for this gene is proI [H]

Identifier: 52786287

GI number: 52786287

Start: 2467110

End: 2468021

Strand: Direct

Name: proI [H]

Synonym: BLi02549

Alternate gene names: 52786287

Gene position: 2467110-2468021 (Clockwise)

Preceding gene: 52786282

Following gene: 52786289

Centisome position: 58.43

GC content: 51.97

Gene sequence:

>912_bases
ATGTATATGCTAATGAAGGTGGTACCGCGAAACCTTTTCGTCCTTTGCGAAGACGGAAAGGTTTTTTTGTTTTTAGAAGG
AGGAATCAAAATGAAACGAATAGGATTTATCGGAGCCGGTTCGATGGCTGAAGCGATGATTAAGGGGCTTGTCAAAAGCG
GTGCAATGGAACCTGAGGCGATATTCGTTACGAATCAGTCGAATACGGCCAGGCTTCGTGAATTAAGCGAGACTTACGGG
GTATGCGGAGAAGCAGATACGGCAAAAGTCGCCGCCCGGTCGGATACACTCGTTTTGGCGATGAAGCCGAAAGACGCGGC
AGGAGGAATAGCCGCACTCCGCCCCTATTTGCGAAAAGAACAGCTGCTGATCTCTGTTCTTGCAGGCATTCCGATTCATA
CGATCCAGCACTATGCCGGGCTTGAGCTGCCTGTTGTGAGAGCCATGCCGAATACATCGGCCGCCATTCAAAAATCAGCC
ACAGCCTTTGCGGCAAGCCCGCTTGTCACGAAAGAGCAGATGCAGGAAGCGGCTGACCTCTTTGCGACGGTCGGCAGCGT
TACAATCGTTGAGGAATCTGCCCTTGATGCTGTGACAGCCGTAGCCGGAAGCGGGCCCGCTTTTGTTTATCGGTTTGTCG
AAGCCCTGCAGGCGTCGGCTGCCGAGCTGGGGCTTCCTGAAAAAACGGCGAAACAGCTGATTATCGATATGATGGCAGGA
GCGGCTCAAATGCTTGAAACCGGCAGAGATCCTTCTGTCATGAGGAAAGAGATCACAAGCGCCGGAGGGACAACGGAAGC
AGGCCTTAGGATGCTTGACGACCATCAATTTGAACAGACCGTCATATCCTGCGTCAAAGAAGCGGCGAATCGTTCTGCGG
AACTCAGGGATATGTTTGCCGCCAAAATTTAA

Upstream 100 bases:

>100_bases
CACGGCTGAAAGATTTCACAGCCCGCTTGCCTGTTGAACCTGCCCTCAGAGCGCTTAGGCAAACCTAAACGTATCCCGCG
TTACGGGGCAAGAGCTGAGC

Downstream 100 bases:

>100_bases
AAAAGTCGGGAACAGCGTTTTCGCTCGTTCCCGATTTTTCTTCAAGCGGTCCCGTATCTTAGAGAAGCTTGTGTATCGCG
AGCCGGAGGATATCTGGCAG

Product: ProI

Products: NA

Alternate protein names: P5C reductase 2; P5CR 2 [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MYMLMKVVPRNLFVLCEDGKVFLFLEGGIKMKRIGFIGAGSMAEAMIKGLVKSGAMEPEAIFVTNQSNTARLRELSETYG
VCGEADTAKVAARSDTLVLAMKPKDAAGGIAALRPYLRKEQLLISVLAGIPIHTIQHYAGLELPVVRAMPNTSAAIQKSA
TAFAASPLVTKEQMQEAADLFATVGSVTIVEESALDAVTAVAGSGPAFVYRFVEALQASAAELGLPEKTAKQLIIDMMAG
AAQMLETGRDPSVMRKEITSAGGTTEAGLRMLDDHQFEQTVISCVKEAANRSAELRDMFAAKI

Sequences:

>Translated_303_residues
MYMLMKVVPRNLFVLCEDGKVFLFLEGGIKMKRIGFIGAGSMAEAMIKGLVKSGAMEPEAIFVTNQSNTARLRELSETYG
VCGEADTAKVAARSDTLVLAMKPKDAAGGIAALRPYLRKEQLLISVLAGIPIHTIQHYAGLELPVVRAMPNTSAAIQKSA
TAFAASPLVTKEQMQEAADLFATVGSVTIVEESALDAVTAVAGSGPAFVYRFVEALQASAAELGLPEKTAKQLIIDMMAG
AAQMLETGRDPSVMRKEITSAGGTTEAGLRMLDDHQFEQTVISCVKEAANRSAELRDMFAAKI
>Mature_303_residues
MYMLMKVVPRNLFVLCEDGKVFLFLEGGIKMKRIGFIGAGSMAEAMIKGLVKSGAMEPEAIFVTNQSNTARLRELSETYG
VCGEADTAKVAARSDTLVLAMKPKDAAGGIAALRPYLRKEQLLISVLAGIPIHTIQHYAGLELPVVRAMPNTSAAIQKSA
TAFAASPLVTKEQMQEAADLFATVGSVTIVEESALDAVTAVAGSGPAFVYRFVEALQASAAELGLPEKTAKQLIIDMMAG
AAQMLETGRDPSVMRKEITSAGGTTEAGLRMLDDHQFEQTVISCVKEAANRSAELRDMFAAKI

Specific function: Proline biosynthesis; third (last) step. [C]

COG id: COG0345

COG function: function code E; Pyrroline-5-carboxylate reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyrroline-5-carboxylate reductase family [H]

Homologues:

Organism=Homo sapiens, GI21361454, Length=273, Percent_Identity=30.03663003663, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI198041662, Length=267, Percent_Identity=29.9625468164794, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI24797095, Length=273, Percent_Identity=29.3040293040293, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI24797097, Length=273, Percent_Identity=29.3040293040293, Blast_Score=112, Evalue=3e-25,
Organism=Escherichia coli, GI1786585, Length=265, Percent_Identity=30.5660377358491, Blast_Score=140, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17569021, Length=261, Percent_Identity=32.183908045977, Blast_Score=142, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17540664, Length=279, Percent_Identity=27.2401433691756, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6320861, Length=284, Percent_Identity=28.169014084507, Blast_Score=102, Evalue=9e-23,
Organism=Drosophila melanogaster, GI24648116, Length=276, Percent_Identity=34.0579710144928, Blast_Score=142, Evalue=2e-34,
Organism=Drosophila melanogaster, GI21358587, Length=264, Percent_Identity=30.3030303030303, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24647700, Length=168, Percent_Identity=32.7380952380952, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR016040
- InterPro:   IPR004455
- InterPro:   IPR000304 [H]

Pfam domain/function: PF03807 F420_oxidored [H]

EC number: =1.5.1.2 [H]

Molecular weight: Translated: 32248; Mature: 32248

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYMLMKVVPRNLFVLCEDGKVFLFLEGGIKMKRIGFIGAGSMAEAMIKGLVKSGAMEPEA
CCHHHEECCCCEEEEEECCCEEEEEECCCEEEEEEEECCCHHHHHHHHHHHHHCCCCCCE
IFVTNQSNTARLRELSETYGVCGEADTAKVAARSDTLVLAMKPKDAAGGIAALRPYLRKE
EEEECCCCHHHHHHHHHHHCCCCCCCHHHEEECCCEEEEEECCCCCCCCHHHHHHHHHHH
QLLISVLAGIPIHTIQHYAGLELPVVRAMPNTSAAIQKSATAFAASPLVTKEQMQEAADL
HHHHHHHHCCCHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHH
FATVGSVTIVEESALDAVTAVAGSGPAFVYRFVEALQASAAELGLPEKTAKQLIIDMMAG
HHHHCCEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
AAQMLETGRDPSVMRKEITSAGGTTEAGLRMLDDHQFEQTVISCVKEAANRSAELRDMFA
HHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHH
AKI
HCC
>Mature Secondary Structure
MYMLMKVVPRNLFVLCEDGKVFLFLEGGIKMKRIGFIGAGSMAEAMIKGLVKSGAMEPEA
CCHHHEECCCCEEEEEECCCEEEEEECCCEEEEEEEECCCHHHHHHHHHHHHHCCCCCCE
IFVTNQSNTARLRELSETYGVCGEADTAKVAARSDTLVLAMKPKDAAGGIAALRPYLRKE
EEEECCCCHHHHHHHHHHHCCCCCCCHHHEEECCCEEEEEECCCCCCCCHHHHHHHHHHH
QLLISVLAGIPIHTIQHYAGLELPVVRAMPNTSAAIQKSATAFAASPLVTKEQMQEAADL
HHHHHHHHCCCHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHH
FATVGSVTIVEESALDAVTAVAGSGPAFVYRFVEALQASAAELGLPEKTAKQLIIDMMAG
HHHHCCEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
AAQMLETGRDPSVMRKEITSAGGTTEAGLRMLDDHQFEQTVISCVKEAANRSAELRDMFA
HHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHH
AKI
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 11418582 [H]