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The map label for this gene is nikA [H]

Identifier: 52786265

GI number: 52786265

Start: 2447855

End: 2449384

Strand: Direct

Name: nikA [H]

Synonym: BLi02527

Alternate gene names: 52786265

Gene position: 2447855-2449384 (Clockwise)

Preceding gene: 52786264

Following gene: 52786266

Centisome position: 57.97

GC content: 46.14

Gene sequence:

>1530_bases
ATGGCTTACATAGCAAAACGCATGATCATACCGATTATTTTTCTCTTCATTCTCGCTTCCTGCTCCGCCGGAGGCGCTGG
CTCAGCGAAAGGGGAAGAGAAGAAACTCACCTTTCTTTTCAATATTCCGAGTCAAACCCTTGACCCGAATCTTGATGTCA
ATTATACGGCTATCCGCGCCGGCATCAGTGAAACACTCGTCAAAATCAGTGCGGACCTTTCCATCGAACCGTGGATTGCC
AAAGATTGGAAAAGCAAAGACGGACAAACTTGGGTCTTCACCCTTAAAGACAATCTGACGTTTCAAAATGGTAAAAAAGC
GGATGCCAAAGCGGTAAAAGCGTCTTTAGAACGGACGATGCGCGAGAGTAAAGCCATGAAGAACGCCCTAAAAATCAAGG
AAATAAAGGCTGAAGGACAAACATTGACGATTACAACGAAGGAGCCGTTTCCCGAATTCCCTTCAGAATTGGTTCATCCA
AACACATCGATCATTGACGTCAGCGCAGCAAACATTTCTCAACAGCCCGTCGGAACGGGACCTTTCCAAGTTTCCTCTTT
TGAGGCCGGCCATAAAATAGAGTTAGAACGGTATGACGATTATTGGGACGGCAAACCGAAGCTGAAGCACGTCACATTCT
CGTTTAATGAAGACGCAAACGCCCGCGTCATGGCTCTCCAATCAAAAGATGCCGATATCATTTACAGACCGAGCGTTGAG
GATATTGACCAAATTCAAAAAGATTCGTCGATTACCGTTGATTCGGTACCGAGCTTGCGCGTTCACCAGATTCTTTACAA
TACAAAGAAGGATCATCTTGCTGACCGCCACCTGCGCCGCGCCTTCGACGCACTACTGAACCGCAAAGAAATCGCTGAAA
GCATTTTGAACGGACATGCTCAGCCGGCAGACGGCCCCTTCCTAGCCGATTTCCCATTTTCCTCCGATACGGTTCAAAAG
CCGAGCGGACTTCAGGCTGCAAAAGCTGAGCTGAAAAAAGCCGGCTACGAGCTTGAAAACGGTAAAGCCGTAAAGGACGG
AAAAGCCTTGTCTTTTACACTGCTAACCTATCAGTCTCGTCCGGAATTGCCTTTAATCGCGCAAATTCTTGAATCAAATG
CGAAGGAATTGGGCATTTCGATTAAGATTCAGCAAGTCGAAAATATTGATGAATATTTGGCGAAAAACGATGACTGGGAT
TTAGCGACATACAGCTCAATGACCGCGCCAAGAGGCGATGCCAGCTATTTGCTGAACACCGCCTATATGCCGAACGGCGC
TTTAAACTACAGCGGCATCGAAAATCAAACCTTGATCAAATGGATTAAGGAATTCAACCGCACGATCGACAAAGAGAAGC
GGAACCGACTTGCCAAAAAAGCAGCTGAATTGATCGAGAAGGAGACATTGAACTCTTTTATCGTGACTCCCGAAAACATC
ACAGCCTACCGAGACGGTGTATTAAATTGGGAAACGAGCAAAAGTGAGTATTATATGCTGACGAAAGATTTGGATGTGAA
AACGAAATGA

Upstream 100 bases:

>100_bases
CTTTCGTCATATAAAGGCAAATATTCCTGTTTACAACAATGAGAATATCCCCTATGATTATGAGGTAAATCGAAATCATT
ACTACTTGGAGGTATAACTT

Downstream 100 bases:

>100_bases
AGCAGATCGCCAAGCGGCTGTTTGAACTGGTCCTGTTCTTATTCCTGCTTTCATTTATCAGCTTTGTGTTTATGAAAGCG
GCTCCCGGTGATCCGGTGAA

Product: hypothetical protein

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 509; Mature: 508

Protein sequence:

>509_residues
MAYIAKRMIIPIIFLFILASCSAGGAGSAKGEEKKLTFLFNIPSQTLDPNLDVNYTAIRAGISETLVKISADLSIEPWIA
KDWKSKDGQTWVFTLKDNLTFQNGKKADAKAVKASLERTMRESKAMKNALKIKEIKAEGQTLTITTKEPFPEFPSELVHP
NTSIIDVSAANISQQPVGTGPFQVSSFEAGHKIELERYDDYWDGKPKLKHVTFSFNEDANARVMALQSKDADIIYRPSVE
DIDQIQKDSSITVDSVPSLRVHQILYNTKKDHLADRHLRRAFDALLNRKEIAESILNGHAQPADGPFLADFPFSSDTVQK
PSGLQAAKAELKKAGYELENGKAVKDGKALSFTLLTYQSRPELPLIAQILESNAKELGISIKIQQVENIDEYLAKNDDWD
LATYSSMTAPRGDASYLLNTAYMPNGALNYSGIENQTLIKWIKEFNRTIDKEKRNRLAKKAAELIEKETLNSFIVTPENI
TAYRDGVLNWETSKSEYYMLTKDLDVKTK

Sequences:

>Translated_509_residues
MAYIAKRMIIPIIFLFILASCSAGGAGSAKGEEKKLTFLFNIPSQTLDPNLDVNYTAIRAGISETLVKISADLSIEPWIA
KDWKSKDGQTWVFTLKDNLTFQNGKKADAKAVKASLERTMRESKAMKNALKIKEIKAEGQTLTITTKEPFPEFPSELVHP
NTSIIDVSAANISQQPVGTGPFQVSSFEAGHKIELERYDDYWDGKPKLKHVTFSFNEDANARVMALQSKDADIIYRPSVE
DIDQIQKDSSITVDSVPSLRVHQILYNTKKDHLADRHLRRAFDALLNRKEIAESILNGHAQPADGPFLADFPFSSDTVQK
PSGLQAAKAELKKAGYELENGKAVKDGKALSFTLLTYQSRPELPLIAQILESNAKELGISIKIQQVENIDEYLAKNDDWD
LATYSSMTAPRGDASYLLNTAYMPNGALNYSGIENQTLIKWIKEFNRTIDKEKRNRLAKKAAELIEKETLNSFIVTPENI
TAYRDGVLNWETSKSEYYMLTKDLDVKTK
>Mature_508_residues
AYIAKRMIIPIIFLFILASCSAGGAGSAKGEEKKLTFLFNIPSQTLDPNLDVNYTAIRAGISETLVKISADLSIEPWIAK
DWKSKDGQTWVFTLKDNLTFQNGKKADAKAVKASLERTMRESKAMKNALKIKEIKAEGQTLTITTKEPFPEFPSELVHPN
TSIIDVSAANISQQPVGTGPFQVSSFEAGHKIELERYDDYWDGKPKLKHVTFSFNEDANARVMALQSKDADIIYRPSVED
IDQIQKDSSITVDSVPSLRVHQILYNTKKDHLADRHLRRAFDALLNRKEIAESILNGHAQPADGPFLADFPFSSDTVQKP
SGLQAAKAELKKAGYELENGKAVKDGKALSFTLLTYQSRPELPLIAQILESNAKELGISIKIQQVENIDEYLAKNDDWDL
ATYSSMTAPRGDASYLLNTAYMPNGALNYSGIENQTLIKWIKEFNRTIDKEKRNRLAKKAAELIEKETLNSFIVTPENIT
AYRDGVLNWETSKSEYYMLTKDLDVKTK

Specific function: Involved in a nickel transport system, probably represents the nickel binder [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789887, Length=471, Percent_Identity=27.1762208067941, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI1787762, Length=453, Percent_Identity=26.0485651214128, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1787052, Length=450, Percent_Identity=24.6666666666667, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1789966, Length=486, Percent_Identity=24.4855967078189, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1787551, Length=261, Percent_Identity=24.5210727969349, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI87081878, Length=396, Percent_Identity=24.7474747474747, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1787495, Length=523, Percent_Identity=22.9445506692161, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011980
- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 56969; Mature: 56838

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYIAKRMIIPIIFLFILASCSAGGAGSAKGEEKKLTFLFNIPSQTLDPNLDVNYTAIRA
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHH
GISETLVKISADLSIEPWIAKDWKSKDGQTWVFTLKDNLTFQNGKKADAKAVKASLERTM
CHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECCCEECCCCCHHHHHHHHHHHHHH
RESKAMKNALKIKEIKAEGQTLTITTKEPFPEFPSELVHPNTSIIDVSAANISQQPVGTG
HHHHHHHHHHHHHHEECCCCEEEEEECCCCCCCCHHHCCCCCCEEEEECCCCCCCCCCCC
PFQVSSFEAGHKIELERYDDYWDGKPKLKHVTFSFNEDANARVMALQSKDADIIYRPSVE
CEEEECCCCCCEEEEEECCCCCCCCCCEEEEEEEECCCCCCEEEEEECCCCCEEECCCHH
DIDQIQKDSSITVDSVPSLRVHQILYNTKKDHLADRHLRRAFDALLNRKEIAESILNGHA
HHHHHHCCCCEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCC
QPADGPFLADFPFSSDTVQKPSGLQAAKAELKKAGYELENGKAVKDGKALSFTLLTYQSR
CCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEEECCCCEEEEEEEEECCC
PELPLIAQILESNAKELGISIKIQQVENIDEYLAKNDDWDLATYSSMTAPRGDASYLLNT
CCCHHHHHHHHCCHHHCCEEEEEHHHHCHHHHHHCCCCCCEEECCCCCCCCCCHHHEEEE
AYMPNGALNYSGIENQTLIKWIKEFNRTIDKEKRNRLAKKAAELIEKETLNSFIVTPENI
EECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCC
TAYRDGVLNWETSKSEYYMLTKDLDVKTK
HHHHCCCCCEECCCCCEEEEEECCCCCCC
>Mature Secondary Structure 
AYIAKRMIIPIIFLFILASCSAGGAGSAKGEEKKLTFLFNIPSQTLDPNLDVNYTAIRA
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHH
GISETLVKISADLSIEPWIAKDWKSKDGQTWVFTLKDNLTFQNGKKADAKAVKASLERTM
CHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECCCEECCCCCHHHHHHHHHHHHHH
RESKAMKNALKIKEIKAEGQTLTITTKEPFPEFPSELVHPNTSIIDVSAANISQQPVGTG
HHHHHHHHHHHHHHEECCCCEEEEEECCCCCCCCHHHCCCCCCEEEEECCCCCCCCCCCC
PFQVSSFEAGHKIELERYDDYWDGKPKLKHVTFSFNEDANARVMALQSKDADIIYRPSVE
CEEEECCCCCCEEEEEECCCCCCCCCCEEEEEEEECCCCCCEEEEEECCCCCEEECCCHH
DIDQIQKDSSITVDSVPSLRVHQILYNTKKDHLADRHLRRAFDALLNRKEIAESILNGHA
HHHHHHCCCCEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCC
QPADGPFLADFPFSSDTVQKPSGLQAAKAELKKAGYELENGKAVKDGKALSFTLLTYQSR
CCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEEECCCCEEEEEEEEECCC
PELPLIAQILESNAKELGISIKIQQVENIDEYLAKNDDWDLATYSSMTAPRGDASYLLNT
CCCHHHHHHHHCCHHHCCEEEEEHHHHCHHHHHHCCCCCCEEECCCCCCCCCCHHHEEEE
AYMPNGALNYSGIENQTLIKWIKEFNRTIDKEKRNRLAKKAAELIEKETLNSFIVTPENI
EECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCC
TAYRDGVLNWETSKSEYYMLTKDLDVKTK
HHHHCCCCCEECCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7934931; 8041620; 9278503 [H]