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The map label for this gene is pucI [H]

Identifier: 52786247

GI number: 52786247

Start: 2436059

End: 2436928

Strand: Direct

Name: pucI [H]

Synonym: BLi02508

Alternate gene names: 52786247

Gene position: 2436059-2436928 (Clockwise)

Preceding gene: 52786246

Following gene: 52786248

Centisome position: 57.69

GC content: 50.34

Gene sequence:

>870_bases
GTGGGCGGTCGATATTGCAGGGGGGCTCGGTCCGATTTATTCGCAGCCGGGAAAATTTCAGACATTCAGCGAATTAATTT
GGCCATTTCTTGCCGGTGTGACGGGGATTATCGGCATTTGGGCGACGCTCATTTTGAACATTCCCGATTTTACGAGGTTT
GCAGCCTCCCAAAAAGAACAAATCAGAGGACAGTTTTACGGTTTGCCGGGCACATTCGTTCTGCAAGCATCACCGTCACC
TCCGGCTCACAGGCTGCCTTCGGGGAGCCGATTTGGGACGTGGTCGATATTCTCGCCCATTTTGACAATCCGTACGTGAT
CGCTCTTTCGGTCATTACGCTTTGCATCGCCGCCATTTCGGTAAACGTTGCGGCAAATATTGTCTCGCCTGCCTATGATC
TGGCCAATTTTTTGCCGAAATACATCGATTTCAAGCGAGGCAGCTATTTGACAGCAGTTTTGGCCCTGTTCACTTTTCCA
TGGAAGCTGATGGAGAATGAAGCGAGTGTGTTTTCGTTTCTCGGCACGATCGGCGGAATATTGGGCCCGGTCGCAGGCGT
TATGCTCGCCGATTATTTCATCATCCGGAAGCGCACCCTCTGCCTCGAAGATCTCTATTCCGCAAAAGGACAGTATATGT
ATTATAAAGGCTATAATTACAGGGCTTTTGCTGCGGTAGCTCTCGGAGCGCTTATATCTCTCATCGGCTCTTACATCCCC
GCCATGAAGCCGCTTTATGACATCTCCTGGTTTTCGGGCGTGCTGATCGCTGCCTTCGCCTATATCTTTTTAATGCGCAT
CCATCCGCCGGCTGCCATTTCAAATGAAACGGCTCAATACGAAGGCGGCGGAAGAAAAATAAGCGGGTAA

Upstream 100 bases:

>100_bases
GGATTCATCTTCTCGTCCTCCATCACGGAATGGAGTCGATTAGAAGGTTTGAAGTCTGGGCCGGCCCGCTCGTTTATCTC
GTCTTTGGCGGGATGGTGTG

Downstream 100 bases:

>100_bases
TATCGAAAAAGGATTTTTCGCTAGTGAAAAATGCCTTTTCTTCTTACCTCTTTTTTTCAAAGGCAGCTTATGATAGACTC
ATATTATCAAAAATTTTCCA

Product: hypothetical protein

Products: NA

Alternate protein names: Allantoin transport protein [H]

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MGGRYCRGARSDLFAAGKISDIQRINLAISCRCDGDYRHLGDAHFEHSRFYEVCSLPKRTNQRTVLRFAGHIRSASITVT
SGSQAAFGEPIWDVVDILAHFDNPYVIALSVITLCIAAISVNVAANIVSPAYDLANFLPKYIDFKRGSYLTAVLALFTFP
WKLMENEASVFSFLGTIGGILGPVAGVMLADYFIIRKRTLCLEDLYSAKGQYMYYKGYNYRAFAAVALGALISLIGSYIP
AMKPLYDISWFSGVLIAAFAYIFLMRIHPPAAISNETAQYEGGGRKISG

Sequences:

>Translated_289_residues
MGGRYCRGARSDLFAAGKISDIQRINLAISCRCDGDYRHLGDAHFEHSRFYEVCSLPKRTNQRTVLRFAGHIRSASITVT
SGSQAAFGEPIWDVVDILAHFDNPYVIALSVITLCIAAISVNVAANIVSPAYDLANFLPKYIDFKRGSYLTAVLALFTFP
WKLMENEASVFSFLGTIGGILGPVAGVMLADYFIIRKRTLCLEDLYSAKGQYMYYKGYNYRAFAAVALGALISLIGSYIP
AMKPLYDISWFSGVLIAAFAYIFLMRIHPPAAISNETAQYEGGGRKISG
>Mature_288_residues
GGRYCRGARSDLFAAGKISDIQRINLAISCRCDGDYRHLGDAHFEHSRFYEVCSLPKRTNQRTVLRFAGHIRSASITVTS
GSQAAFGEPIWDVVDILAHFDNPYVIALSVITLCIAAISVNVAANIVSPAYDLANFLPKYIDFKRGSYLTAVLALFTFPW
KLMENEASVFSFLGTIGGILGPVAGVMLADYFIIRKRTLCLEDLYSAKGQYMYYKGYNYRAFAAVALGALISLIGSYIPA
MKPLYDISWFSGVLIAAFAYIFLMRIHPPAAISNETAQYEGGGRKISG

Specific function: Transport of allantoin [H]

COG id: COG1953

COG function: function code FH; Cytosine/uracil/thiamine/allantoin permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine-cytosine permease family [H]

Homologues:

Organism=Escherichia coli, GI87081749, Length=193, Percent_Identity=36.7875647668394, Blast_Score=149, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6319429, Length=217, Percent_Identity=29.9539170506912, Blast_Score=87, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323266, Length=205, Percent_Identity=27.3170731707317, Blast_Score=85, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6324766, Length=166, Percent_Identity=28.3132530120482, Blast_Score=83, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6324645, Length=209, Percent_Identity=24.8803827751196, Blast_Score=80, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6322219, Length=171, Percent_Identity=30.9941520467836, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6319495, Length=234, Percent_Identity=28.2051282051282, Blast_Score=78, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001248
- InterPro:   IPR012681 [H]

Pfam domain/function: PF02133 Transp_cyt_pur [H]

EC number: NA

Molecular weight: Translated: 31814; Mature: 31683

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGRYCRGARSDLFAAGKISDIQRINLAISCRCDGDYRHLGDAHFEHSRFYEVCSLPKRT
CCCCCCCCCCCCCEECCCCCCCEEEEEEEEEEECCCHHHCCCCHHHHHHHHHHHCCCCCC
NQRTVLRFAGHIRSASITVTSGSQAAFGEPIWDVVDILAHFDNPYVIALSVITLCIAAIS
CHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
VNVAANIVSPAYDLANFLPKYIDFKRGSYLTAVLALFTFPWKLMENEASVFSFLGTIGGI
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
LGPVAGVMLADYFIIRKRTLCLEDLYSAKGQYMYYKGYNYRAFAAVALGALISLIGSYIP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHH
AMKPLYDISWFSGVLIAAFAYIFLMRIHPPAAISNETAQYEGGGRKISG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCC
>Mature Secondary Structure 
GGRYCRGARSDLFAAGKISDIQRINLAISCRCDGDYRHLGDAHFEHSRFYEVCSLPKRT
CCCCCCCCCCCCEECCCCCCCEEEEEEEEEEECCCHHHCCCCHHHHHHHHHHHCCCCCC
NQRTVLRFAGHIRSASITVTSGSQAAFGEPIWDVVDILAHFDNPYVIALSVITLCIAAIS
CHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
VNVAANIVSPAYDLANFLPKYIDFKRGSYLTAVLALFTFPWKLMENEASVFSFLGTIGGI
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
LGPVAGVMLADYFIIRKRTLCLEDLYSAKGQYMYYKGYNYRAFAAVALGALISLIGSYIP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHH
AMKPLYDISWFSGVLIAAFAYIFLMRIHPPAAISNETAQYEGGGRKISG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 11344136 [H]