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The map label for this gene is yclK [H]

Identifier: 52786194

GI number: 52786194

Start: 2390174

End: 2391625

Strand: Reverse

Name: yclK [H]

Synonym: BLi02454

Alternate gene names: 52786194

Gene position: 2391625-2390174 (Counterclockwise)

Preceding gene: 52786195

Following gene: 52786193

Centisome position: 56.64

GC content: 40.29

Gene sequence:

>1452_bases
GTGTTACGGACCATCAAGGGCAAGTTTATTGTTGGCTTTTTTCTGATTTTTTCTCTCTCTTTCCTTGTGCTCAATCACAC
GGTTAAGAACGTCATTTGGTCGAGTAACGAAAAAATTGTAACATCGGATTTAGTCGAACTGAAAAAAAACAGTACCGTCT
ATGTAAACCAAGCATTTTTAATCAATCACTATAAAAACAATGAACTTTATTTCGGTGATATGGCCGATGAAATGGTAGAT
AATTTAAATCACTCCACAGGAAGCAATGTCGGTGTTTATACAGTAGATGGCGCATTGTTGTCTTCTTCTGATCAGAAGGT
GTTTTCCAGAGGGAGTGATGAAGATCTTCAACAGGCGATCAAAGGAAAGACTGCCTATCATATCACCTATAACCGGAATA
AAGCAAAAGTGTTTTTCTCATATCCGGTTGTCATTGAAGGCACAAAAGTAGGCATATTACGCTTTTCGAAAAATTTCGAC
CTGCTGTATAAGCAGAGCGGAGAGATTCTGGATATTGTTTTTTATATCGCGCTTGCAATATTTGTGGCGGCGTTCCTTTT
CTCCTATATCCTGTCCAGACATATCACGATTCCGCTCGTCAAGCTGACAGATGCCACCTCACAAGTCAAGAAGGGTAAGC
TGGATATGGATATCCACTTTAATAGAAAAGATGAGATTGGCCAGCTTGCCGTCAACTTTAACGATATGATCAGCAGGATT
CGGGAACAAATTTTAACCATCGAAAGGGATCGCGACCGTCTAAAAGAACTTAACGAACAAGAAAAACGGTTTTTTGATAA
TATGACGCATGAGCTGAAAACACCTCTGACTTCCATCCAAGGATATGCTGAGATCATAAAAGATAAAGGGGAAGCGGACC
GGAAATTTTTTGATAAAGGAATGAACCACATTGTCGAAGAAAGCCGGCGCCTGCATAATTTGGTGATCAAGCTTCTTGAA
GTATCCCGGGAAACGTCCAAGAGTGCTCAATTTGACAGGATCGACGCAGGCGAAATTTTAAAAGATGTTTGCGAGTCGAT
GAAATTTCGGGCAGAACGGTATAAGAAAAGGATCACCTGTGACATTGAAGATGGCCTGTACGTTCTTGGTGAGGTGAGCA
GGCTGCGCCAGCTTTTTATCAATCTGCTGGACAACGCCATCAAATACAGCTTATCTCATTCGGAAATCATCATTAAGGCC
GAAATCGTTGGAGAAAACATTCTTTTTACATTCAAGAATCCAAGCGATCCATTGGAAAATGACCAACACACCAAAATGTT
CCAACCTTTTTATTCAGTCAATCAGAAGATTAAGGAAGAAGGAAGTGTAGGTTTGGGATTAAGCATTGTGAAAACAATTG
TTGACGAACACGGAGGAACAATCGAGATGTTCTACAAAGAAAACCACACAGTGATTCAAGTTGAATTAGCTTACGCAAAG
GTTGAGAAATGA

Upstream 100 bases:

>100_bases
TACCCATATTCAAAGACTCCGCAAAAAGCTTGATGCAAGCGACTTGATTAAAACAGTGTTCGGCATCGGATATAAATTCG
AGAAGCAGGAGGAGTAGAGA

Downstream 100 bases:

>100_bases
TGAAAAACAAACCGTTTATTATATGGGCAGGAGTGCTGCTGTTTCTGATCATTATCGTGTCCAGTTATAATGTCGGAAAA
AGATCGGAAACGATTATTAT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MLRTIKGKFIVGFFLIFSLSFLVLNHTVKNVIWSSNEKIVTSDLVELKKNSTVYVNQAFLINHYKNNELYFGDMADEMVD
NLNHSTGSNVGVYTVDGALLSSSDQKVFSRGSDEDLQQAIKGKTAYHITYNRNKAKVFFSYPVVIEGTKVGILRFSKNFD
LLYKQSGEILDIVFYIALAIFVAAFLFSYILSRHITIPLVKLTDATSQVKKGKLDMDIHFNRKDEIGQLAVNFNDMISRI
REQILTIERDRDRLKELNEQEKRFFDNMTHELKTPLTSIQGYAEIIKDKGEADRKFFDKGMNHIVEESRRLHNLVIKLLE
VSRETSKSAQFDRIDAGEILKDVCESMKFRAERYKKRITCDIEDGLYVLGEVSRLRQLFINLLDNAIKYSLSHSEIIIKA
EIVGENILFTFKNPSDPLENDQHTKMFQPFYSVNQKIKEEGSVGLGLSIVKTIVDEHGGTIEMFYKENHTVIQVELAYAK
VEK

Sequences:

>Translated_483_residues
MLRTIKGKFIVGFFLIFSLSFLVLNHTVKNVIWSSNEKIVTSDLVELKKNSTVYVNQAFLINHYKNNELYFGDMADEMVD
NLNHSTGSNVGVYTVDGALLSSSDQKVFSRGSDEDLQQAIKGKTAYHITYNRNKAKVFFSYPVVIEGTKVGILRFSKNFD
LLYKQSGEILDIVFYIALAIFVAAFLFSYILSRHITIPLVKLTDATSQVKKGKLDMDIHFNRKDEIGQLAVNFNDMISRI
REQILTIERDRDRLKELNEQEKRFFDNMTHELKTPLTSIQGYAEIIKDKGEADRKFFDKGMNHIVEESRRLHNLVIKLLE
VSRETSKSAQFDRIDAGEILKDVCESMKFRAERYKKRITCDIEDGLYVLGEVSRLRQLFINLLDNAIKYSLSHSEIIIKA
EIVGENILFTFKNPSDPLENDQHTKMFQPFYSVNQKIKEEGSVGLGLSIVKTIVDEHGGTIEMFYKENHTVIQVELAYAK
VEK
>Mature_483_residues
MLRTIKGKFIVGFFLIFSLSFLVLNHTVKNVIWSSNEKIVTSDLVELKKNSTVYVNQAFLINHYKNNELYFGDMADEMVD
NLNHSTGSNVGVYTVDGALLSSSDQKVFSRGSDEDLQQAIKGKTAYHITYNRNKAKVFFSYPVVIEGTKVGILRFSKNFD
LLYKQSGEILDIVFYIALAIFVAAFLFSYILSRHITIPLVKLTDATSQVKKGKLDMDIHFNRKDEIGQLAVNFNDMISRI
REQILTIERDRDRLKELNEQEKRFFDNMTHELKTPLTSIQGYAEIIKDKGEADRKFFDKGMNHIVEESRRLHNLVIKLLE
VSRETSKSAQFDRIDAGEILKDVCESMKFRAERYKKRITCDIEDGLYVLGEVSRLRQLFINLLDNAIKYSLSHSEIIIKA
EIVGENILFTFKNPSDPLENDQHTKMFQPFYSVNQKIKEEGSVGLGLSIVKTIVDEHGGTIEMFYKENHTVIQVELAYAK
VEK

Specific function: Could be member of the two-component regulatory system yclK/yclJ. Potentially phosphorylates yclJ [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=330, Percent_Identity=27.5757575757576, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1790346, Length=270, Percent_Identity=27.037037037037, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1786600, Length=219, Percent_Identity=26.9406392694064, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1786912, Length=244, Percent_Identity=27.8688524590164, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI87082128, Length=313, Percent_Identity=22.0447284345048, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1786783, Length=254, Percent_Identity=25.5905511811024, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI87081816, Length=307, Percent_Identity=22.8013029315961, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1790436, Length=232, Percent_Identity=26.7241379310345, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789149, Length=330, Percent_Identity=25.4545454545455, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1790861, Length=230, Percent_Identity=26.9565217391304, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1787894, Length=306, Percent_Identity=23.8562091503268, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1790551, Length=320, Percent_Identity=23.75, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1789808, Length=300, Percent_Identity=24, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1788279, Length=261, Percent_Identity=23.3716475095785, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 55530; Mature: 55530

Theoretical pI: Translated: 7.46; Mature: 7.46

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRTIKGKFIVGFFLIFSLSFLVLNHTVKNVIWSSNEKIVTSDLVELKKNSTVYVNQAFL
CCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHCCCCEEEEEEEEE
INHYKNNELYFGDMADEMVDNLNHSTGSNVGVYTVDGALLSSSDQKVFSRGSDEDLQQAI
EEEECCCEEEECCHHHHHHHHCCCCCCCCCEEEEECCHHCCCCCHHHHHCCCCHHHHHHH
KGKTAYHITYNRNKAKVFFSYPVVIEGTKVGILRFSKNFDLLYKQSGEILDIVFYIALAI
CCCEEEEEEEECCCEEEEEECCEEEECCEEEEEEECCCCCEEECCCCCHHHHHHHHHHHH
FVAAFLFSYILSRHITIPLVKLTDATSQVKKGKLDMDIHFNRKDEIGQLAVNFNDMISRI
HHHHHHHHHHHHCCCEEEEEEECCCHHHHHCCCCCEEEEECCCCCHHHEEECHHHHHHHH
REQILTIERDRDRLKELNEQEKRFFDNMTHELKTPLTSIQGYAEIIKDKGEADRKFFDKG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCHHHHHHHHH
MNHIVEESRRLHNLVIKLLEVSRETSKSAQFDRIDAGEILKDVCESMKFRAERYKKRITC
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEE
DIEDGLYVLGEVSRLRQLFINLLDNAIKYSLSHSEIIIKAEIVGENILFTFKNPSDPLEN
EECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCEEEEEECCCCCCCCC
DQHTKMFQPFYSVNQKIKEEGSVGLGLSIVKTIVDEHGGTIEMFYKENHTVIQVELAYAK
CCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEEEECCCEEEEEEEEEEE
VEK
CCC
>Mature Secondary Structure
MLRTIKGKFIVGFFLIFSLSFLVLNHTVKNVIWSSNEKIVTSDLVELKKNSTVYVNQAFL
CCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHCCCCEEEEEEEEE
INHYKNNELYFGDMADEMVDNLNHSTGSNVGVYTVDGALLSSSDQKVFSRGSDEDLQQAI
EEEECCCEEEECCHHHHHHHHCCCCCCCCCEEEEECCHHCCCCCHHHHHCCCCHHHHHHH
KGKTAYHITYNRNKAKVFFSYPVVIEGTKVGILRFSKNFDLLYKQSGEILDIVFYIALAI
CCCEEEEEEEECCCEEEEEECCEEEECCEEEEEEECCCCCEEECCCCCHHHHHHHHHHHH
FVAAFLFSYILSRHITIPLVKLTDATSQVKKGKLDMDIHFNRKDEIGQLAVNFNDMISRI
HHHHHHHHHHHHCCCEEEEEEECCCHHHHHCCCCCEEEEECCCCCHHHEEECHHHHHHHH
REQILTIERDRDRLKELNEQEKRFFDNMTHELKTPLTSIQGYAEIIKDKGEADRKFFDKG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCHHHHHHHHH
MNHIVEESRRLHNLVIKLLEVSRETSKSAQFDRIDAGEILKDVCESMKFRAERYKKRITC
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEE
DIEDGLYVLGEVSRLRQLFINLLDNAIKYSLSHSEIIIKAEIVGENILFTFKNPSDPLEN
EECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCEEEEEECCCCCCCCC
DQHTKMFQPFYSVNQKIKEEGSVGLGLSIVKTIVDEHGGTIEMFYKENHTVIQVELAYAK
CCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEEEECCCEEEEEEEEEEE
VEK
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]