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The map label for this gene is trpE [H]

Identifier: 52786147

GI number: 52786147

Start: 2346570

End: 2348117

Strand: Reverse

Name: trpE [H]

Synonym: BLi02403

Alternate gene names: 52786147

Gene position: 2348117-2346570 (Counterclockwise)

Preceding gene: 52786148

Following gene: 52786146

Centisome position: 55.61

GC content: 48.77

Gene sequence:

>1548_bases
TTGAGTCTGCAAACCGATTTTGCCTCTTTTTCAGAGGATTCTTCACACTTTAAAACGATACCGATTATTGAGAGTTTTTC
CGTCGATACGCTGACGCCCGTTCAATTGGTCGAGAAGCTGCAGGAAGACATCGTCTATTTGCTGGAAAGCCAGGATGAAA
CCTCTGCATGGTCAAGATATTCTTTCATCGGCCTTGACCCCTTTTTGACGATCAAAGAAGAACGCGGCCGGTTTCACGCC
TGCGATCAAAAGCAGAAGAAGCTTTATTCGGCTTCACAGTTAAAAGACGTTCTCGAATGGATGTCGGAGACGTATTCGAT
CAAAGCGCCTGAGCTCGACATCCCGTTTACAGGAGGAGCGGTCGGCTATTTAAGCTACGATTTAATGCCGCTCATTGAAC
CGTCCATCAAAGGGCACAGCAAAGAAACGGAAATGGCAAAATGCCTCCTGTTTGTCTGCCGGACGATGATCGCTTATGAT
CACGAACTGAAAAAGCTGCATTTCATCCACTATACAAGGCTTGAAGGCAGTGAAAATAGGGAGGAAAAGCGCCGCATTTT
CAAAAAGGGGAAAGAAGAGCTCAAGCAGCTTACACACAGGCTGTCAGACCGGAAAACGCCGAAAGAGCTGTTTCTTCCGC
AAGGACGGCACACGGCCCCCAGCTTTGAAGGGATTCAATCAACATATGAGAAATCCCGGTTTTTGGCCGATGTCGATCGC
TTGAAAGAATACATTAAAGCGGGAGACATTTTTCAAGGCGTCCTTTCGCAAAGGTTCGACATTCCGGTGAAGGTCGGTTC
TTTTGAGTTATACAGAGTGCTGAGAATGATCAACCCGTCCCCGTACATGTACTACATGAGGCTTCCTGACCGCGATCTCG
TCGGAAGCTCGCCCGAACGTCTGATCCATGTTCAAAACGGCCATCTTGAAATCCATCCGATCGCCGGCACAAGGAAAAGG
GGCGGTGATCAGGCTGAGGATAAGCGGCTTGAAAAAGAACTATTGGCTGATGAGAAAGAAAAAGCGGAGCATTACATGCT
TGTCGATTTGGCGAGAAATGATATCGGCAGAGTAGCTGAGTACGGGTCTGTCCAAGTGCCCGAGTTTACGAAAATCGTTT
CCTTTTCGCACGTCATGCACATCATTTCGATCGTAACCGGCACTTTAAAAGCGGGTGTCCACCCGGTTGACGCTTTAATG
AGCGCATTTCCGGCCGGAACTTTGACCGGCGCTCCGAAAATCCGCGCGATTCAGCTTTTAAATGAGCTTGAGCCCGAACC
GAGGGAAACATACGGAGGATGCATTGCTTACATCGGATTTGACGGAAATATCGATTCGTGCATCACGATCCGGACGATGA
GCGTGAAAAACGGAGTGGCGTCCATTCAGGCCGGGGCGGGCATCGTAGCCGACTCCGTGCCTGAAAAAGAATGGGAAGAG
ACCTGCAATAAAGCAGGGGCTCTGTTAAAAACGATTCAAATCGCAGAAGAAACGTTTCTCGAAAAGGGGGAGGACGGCAA
TGAACGAACTGCTGAAATCATGCGTTAA

Upstream 100 bases:

>100_bases
GTTCTTTTGGGTTTGTTTCGTTTTCCCCTCGTCAATTCAATCATTTGTCCTCTAAAGGCCTTCTTCTCATTCTCTCCGCC
TGAAAGGGAGTGAGAGATAG

Downstream 100 bases:

>100_bases
CGGACGGACACTGAATGAAATCGAGGCACACAGCATCATGCGCGACATGATGACCGGCTCGCTGAGCGCTGCGGAAATCG
GAGGCCTCCTGTCCGTTCTC

Product: anthranilate synthase component I

Products: NA

Alternate protein names: Anthranilate synthase component I [H]

Number of amino acids: Translated: 515; Mature: 514

Protein sequence:

>515_residues
MSLQTDFASFSEDSSHFKTIPIIESFSVDTLTPVQLVEKLQEDIVYLLESQDETSAWSRYSFIGLDPFLTIKEERGRFHA
CDQKQKKLYSASQLKDVLEWMSETYSIKAPELDIPFTGGAVGYLSYDLMPLIEPSIKGHSKETEMAKCLLFVCRTMIAYD
HELKKLHFIHYTRLEGSENREEKRRIFKKGKEELKQLTHRLSDRKTPKELFLPQGRHTAPSFEGIQSTYEKSRFLADVDR
LKEYIKAGDIFQGVLSQRFDIPVKVGSFELYRVLRMINPSPYMYYMRLPDRDLVGSSPERLIHVQNGHLEIHPIAGTRKR
GGDQAEDKRLEKELLADEKEKAEHYMLVDLARNDIGRVAEYGSVQVPEFTKIVSFSHVMHIISIVTGTLKAGVHPVDALM
SAFPAGTLTGAPKIRAIQLLNELEPEPRETYGGCIAYIGFDGNIDSCITIRTMSVKNGVASIQAGAGIVADSVPEKEWEE
TCNKAGALLKTIQIAEETFLEKGEDGNERTAEIMR

Sequences:

>Translated_515_residues
MSLQTDFASFSEDSSHFKTIPIIESFSVDTLTPVQLVEKLQEDIVYLLESQDETSAWSRYSFIGLDPFLTIKEERGRFHA
CDQKQKKLYSASQLKDVLEWMSETYSIKAPELDIPFTGGAVGYLSYDLMPLIEPSIKGHSKETEMAKCLLFVCRTMIAYD
HELKKLHFIHYTRLEGSENREEKRRIFKKGKEELKQLTHRLSDRKTPKELFLPQGRHTAPSFEGIQSTYEKSRFLADVDR
LKEYIKAGDIFQGVLSQRFDIPVKVGSFELYRVLRMINPSPYMYYMRLPDRDLVGSSPERLIHVQNGHLEIHPIAGTRKR
GGDQAEDKRLEKELLADEKEKAEHYMLVDLARNDIGRVAEYGSVQVPEFTKIVSFSHVMHIISIVTGTLKAGVHPVDALM
SAFPAGTLTGAPKIRAIQLLNELEPEPRETYGGCIAYIGFDGNIDSCITIRTMSVKNGVASIQAGAGIVADSVPEKEWEE
TCNKAGALLKTIQIAEETFLEKGEDGNERTAEIMR
>Mature_514_residues
SLQTDFASFSEDSSHFKTIPIIESFSVDTLTPVQLVEKLQEDIVYLLESQDETSAWSRYSFIGLDPFLTIKEERGRFHAC
DQKQKKLYSASQLKDVLEWMSETYSIKAPELDIPFTGGAVGYLSYDLMPLIEPSIKGHSKETEMAKCLLFVCRTMIAYDH
ELKKLHFIHYTRLEGSENREEKRRIFKKGKEELKQLTHRLSDRKTPKELFLPQGRHTAPSFEGIQSTYEKSRFLADVDRL
KEYIKAGDIFQGVLSQRFDIPVKVGSFELYRVLRMINPSPYMYYMRLPDRDLVGSSPERLIHVQNGHLEIHPIAGTRKRG
GDQAEDKRLEKELLADEKEKAEHYMLVDLARNDIGRVAEYGSVQVPEFTKIVSFSHVMHIISIVTGTLKAGVHPVDALMS
AFPAGTLTGAPKIRAIQLLNELEPEPRETYGGCIAYIGFDGNIDSCITIRTMSVKNGVASIQAGAGIVADSVPEKEWEET
CNKAGALLKTIQIAEETFLEKGEDGNERTAEIMR

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anthranilate synthase component I family [H]

Homologues:

Organism=Escherichia coli, GI1787518, Length=416, Percent_Identity=37.2596153846154, Blast_Score=245, Evalue=4e-66,
Organism=Escherichia coli, GI1788114, Length=448, Percent_Identity=31.25, Blast_Score=224, Evalue=1e-59,
Organism=Escherichia coli, GI1786809, Length=270, Percent_Identity=28.1481481481481, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI87082077, Length=221, Percent_Identity=29.4117647058824, Blast_Score=96, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6320935, Length=461, Percent_Identity=36.6594360086768, Blast_Score=275, Evalue=1e-74,
Organism=Saccharomyces cerevisiae, GI6324361, Length=403, Percent_Identity=25.5583126550869, Blast_Score=109, Evalue=1e-24,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR019999
- InterPro:   IPR006805
- InterPro:   IPR005256
- InterPro:   IPR015890 [H]

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 58326; Mature: 58195

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLQTDFASFSEDSSHFKTIPIIESFSVDTLTPVQLVEKLQEDIVYLLESQDETSAWSRY
CCCCCCHHHCCCCCCCCEEECEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHEE
SFIGLDPFLTIKEERGRFHACDQKQKKLYSASQLKDVLEWMSETYSIKAPELDIPFTGGA
EEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
VGYLSYDLMPLIEPSIKGHSKETEMAKCLLFVCRTMIAYDHELKKLHFIHYTRLEGSENR
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCH
EEKRRIFKKGKEELKQLTHRLSDRKTPKELFLPQGRHTAPSFEGIQSTYEKSRFLADVDR
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LKEYIKAGDIFQGVLSQRFDIPVKVGSFELYRVLRMINPSPYMYYMRLPDRDLVGSSPER
HHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHH
LIHVQNGHLEIHPIAGTRKRGGDQAEDKRLEKELLADEKEKAEHYMLVDLARNDIGRVAE
EEEEECCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHEEEEHHHHHHHHHHHH
YGSVQVPEFTKIVSFSHVMHIISIVTGTLKAGVHPVDALMSAFPAGTLTGAPKIRAIQLL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCHHHHHHHH
NELEPEPRETYGGCIAYIGFDGNIDSCITIRTMSVKNGVASIQAGAGIVADSVPEKEWEE
HHCCCCCCCCCCCEEEEEECCCCCCCEEEEEEEEHHCCHHHHHCCCCCCCCCCCHHHHHH
TCNKAGALLKTIQIAEETFLEKGEDGNERTAEIMR
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCC
>Mature Secondary Structure 
SLQTDFASFSEDSSHFKTIPIIESFSVDTLTPVQLVEKLQEDIVYLLESQDETSAWSRY
CCCCCHHHCCCCCCCCEEECEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHEE
SFIGLDPFLTIKEERGRFHACDQKQKKLYSASQLKDVLEWMSETYSIKAPELDIPFTGGA
EEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
VGYLSYDLMPLIEPSIKGHSKETEMAKCLLFVCRTMIAYDHELKKLHFIHYTRLEGSENR
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCH
EEKRRIFKKGKEELKQLTHRLSDRKTPKELFLPQGRHTAPSFEGIQSTYEKSRFLADVDR
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LKEYIKAGDIFQGVLSQRFDIPVKVGSFELYRVLRMINPSPYMYYMRLPDRDLVGSSPER
HHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHH
LIHVQNGHLEIHPIAGTRKRGGDQAEDKRLEKELLADEKEKAEHYMLVDLARNDIGRVAE
EEEEECCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHEEEEHHHHHHHHHHHH
YGSVQVPEFTKIVSFSHVMHIISIVTGTLKAGVHPVDALMSAFPAGTLTGAPKIRAIQLL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCHHHHHHHH
NELEPEPRETYGGCIAYIGFDGNIDSCITIRTMSVKNGVASIQAGAGIVADSVPEKEWEE
HHCCCCCCCCCCCEEEEEECCCCCCCEEEEEEEEHHCCHHHHHCCCCCCCCCCCHHHHHH
TCNKAGALLKTIQIAEETFLEKGEDGNERTAEIMR
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3924737; 6425119; 9384377; 6436812; 2422155 [H]