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The map label for this gene is birA [H]

Identifier: 52786123

GI number: 52786123

Start: 2325528

End: 2326511

Strand: Reverse

Name: birA [H]

Synonym: BLi02379

Alternate gene names: 52786123

Gene position: 2326511-2325528 (Counterclockwise)

Preceding gene: 52786124

Following gene: 52786122

Centisome position: 55.1

GC content: 47.15

Gene sequence:

>984_bases
ATGCAGTCAAATTTAAGAAAGAAACTCATTCAATTGTTTACCGAGGCAGACGGCGAATTCATTTCTGGCCAGAAAATCAG
CGAAGAGCTCGGATGCTCAAGAACCGCCGTTTGGAAGCATATCGAAGATCTGAGAAAATCGGGATATGAACTTGAAGCGG
TCCGAAAGCTCGGCTATAAAATGATAAAAAAACCCGACAAGATCACTGAAGATGAAATCAGGCTCGGGTTAAAAACGAAA
AAAATGGGACAGACGATCCATTTTCAGGATGTCGTTTCATCAACTCAAAAAATCGCCCACGAACTTGCAAACAACGGTGC
GCCCGAAGGAACGATCATCGTTGCCGACAAGCAGACTGAAGGAAGAGGAAGAATGGCGAGAGCATGGCATTCCCCGGAAG
GAAACGGCATTTGGATGAGCATCATTTTAAGGCCTGAAATTCCCGTGCAAAAAACGCCGCAGCTGACGCTTTTAGCCGCT
GTAGCGATCGTTCAGGCAATCGAAGAGCAAACCGGCATCGCAGCCGAAATTAAATGGCCGAACGATATTTTGATCAACGG
CAAAAAAGTGGTCGGGATCTTAACGGAACTGCAGGCGGAAGCTGATCAGGTCCATTCCGTTATTGTCGGAACGGGGATCA
ACGTCAATCAGCTTGCAGCCGACTTTCCGGAGGAATTACAAGAAACAGCGACAAGCCTTCGTCTCGCAAGCGGTGAAAAA
ATCGACAGAGCGGCTTTGGTCCAAACAATAATGTCGACGTTTGAAAAAAGGTATGAAGACTATCTCGCCTACGGTTTTGA
ACCGATCAAGCTTTTGTGGGAAAGCTATGCTTTGACCCTGAACCGCGAATTGACGGCCCGGACGCTGAACGGAACGTTTC
ACGGCAAAGCGCTCGGAATCGACGAAGAAGGCGTATTGATCCTGGAAACGAGTGACGGCATCAAAAAAATATACTCCGCT
GATATCGATATTAATCCGTCATAG

Upstream 100 bases:

>100_bases
CCGGAAAATGGGTGTCCGATGATTTGAAACGGATTGAGCGGGCCGTGTTAAACGGGGAGCTTGCCAATCAAAAAAAAGCC
ATAGAGGAGTGGCTGGATTC

Downstream 100 bases:

>100_bases
TTGGAACGATCCATGCGTTTTTGGTATACTTCGGATGGGCAGTATCCTCTGGGAACTGCACCTGGAAAGCATGCAACTTA
TAAAAAAACAGTCTGCCTTG

Product: hypothetical protein

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MQSNLRKKLIQLFTEADGEFISGQKISEELGCSRTAVWKHIEDLRKSGYELEAVRKLGYKMIKKPDKITEDEIRLGLKTK
KMGQTIHFQDVVSSTQKIAHELANNGAPEGTIIVADKQTEGRGRMARAWHSPEGNGIWMSIILRPEIPVQKTPQLTLLAA
VAIVQAIEEQTGIAAEIKWPNDILINGKKVVGILTELQAEADQVHSVIVGTGINVNQLAADFPEELQETATSLRLASGEK
IDRAALVQTIMSTFEKRYEDYLAYGFEPIKLLWESYALTLNRELTARTLNGTFHGKALGIDEEGVLILETSDGIKKIYSA
DIDINPS

Sequences:

>Translated_327_residues
MQSNLRKKLIQLFTEADGEFISGQKISEELGCSRTAVWKHIEDLRKSGYELEAVRKLGYKMIKKPDKITEDEIRLGLKTK
KMGQTIHFQDVVSSTQKIAHELANNGAPEGTIIVADKQTEGRGRMARAWHSPEGNGIWMSIILRPEIPVQKTPQLTLLAA
VAIVQAIEEQTGIAAEIKWPNDILINGKKVVGILTELQAEADQVHSVIVGTGINVNQLAADFPEELQETATSLRLASGEK
IDRAALVQTIMSTFEKRYEDYLAYGFEPIKLLWESYALTLNRELTARTLNGTFHGKALGIDEEGVLILETSDGIKKIYSA
DIDINPS
>Mature_327_residues
MQSNLRKKLIQLFTEADGEFISGQKISEELGCSRTAVWKHIEDLRKSGYELEAVRKLGYKMIKKPDKITEDEIRLGLKTK
KMGQTIHFQDVVSSTQKIAHELANNGAPEGTIIVADKQTEGRGRMARAWHSPEGNGIWMSIILRPEIPVQKTPQLTLLAA
VAIVQAIEEQTGIAAEIKWPNDILINGKKVVGILTELQAEADQVHSVIVGTGINVNQLAADFPEELQETATSLRLASGEK
IDRAALVQTIMSTFEKRYEDYLAYGFEPIKLLWESYALTLNRELTARTLNGTFHGKALGIDEEGVLILETSDGIKKIYSA
DIDINPS

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Homo sapiens, GI46255045, Length=150, Percent_Identity=34, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1790408, Length=313, Percent_Identity=29.3929712460064, Blast_Score=117, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI32563942, Length=260, Percent_Identity=27.3076923076923, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI32563944, Length=254, Percent_Identity=27.9527559055118, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI212645369, Length=336, Percent_Identity=25.297619047619, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 36294; Mature: 36294

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS00041 HTH_ARAC_FAMILY_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSNLRKKLIQLFTEADGEFISGQKISEELGCSRTAVWKHIEDLRKSGYELEAVRKLGYK
CCCHHHHHHHHHHHHCCCCEECCCHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHH
MIKKPDKITEDEIRLGLKTKKMGQTIHFQDVVSSTQKIAHELANNGAPEGTIIVADKQTE
HHCCCCCCCHHHHHHCCCHHHHCCEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCC
GRGRMARAWHSPEGNGIWMSIILRPEIPVQKTPQLTLLAAVAIVQAIEEQTGIAAEIKWP
CCCCHHHHCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECC
NDILINGKKVVGILTELQAEADQVHSVIVGTGINVNQLAADFPEELQETATSLRLASGEK
CCEEECCHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHCCHHHHHHHHHHHHHCCCCC
IDRAALVQTIMSTFEKRYEDYLAYGFEPIKLLWESYALTLNRELTARTLNGTFHGKALGI
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEECCCCEEEEECCCCCCEEECC
DEEGVLILETSDGIKKIYSADIDINPS
CCCCEEEEECCCHHHHHHHCCCCCCCC
>Mature Secondary Structure
MQSNLRKKLIQLFTEADGEFISGQKISEELGCSRTAVWKHIEDLRKSGYELEAVRKLGYK
CCCHHHHHHHHHHHHCCCCEECCCHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHH
MIKKPDKITEDEIRLGLKTKKMGQTIHFQDVVSSTQKIAHELANNGAPEGTIIVADKQTE
HHCCCCCCCHHHHHHCCCHHHHCCEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCC
GRGRMARAWHSPEGNGIWMSIILRPEIPVQKTPQLTLLAAVAIVQAIEEQTGIAAEIKWP
CCCCHHHHCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECC
NDILINGKKVVGILTELQAEADQVHSVIVGTGINVNQLAADFPEELQETATSLRLASGEK
CCEEECCHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHCCHHHHHHHHHHHHHCCCCC
IDRAALVQTIMSTFEKRYEDYLAYGFEPIKLLWESYALTLNRELTARTLNGTFHGKALGI
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEECCCCEEEEECCCCCCEEECC
DEEGVLILETSDGIKKIYSADIDINPS
CCCCEEEEECCCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]