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The map label for this gene is panB

Identifier: 52786122

GI number: 52786122

Start: 2324445

End: 2325278

Strand: Reverse

Name: panB

Synonym: BLi02378

Alternate gene names: 52786122

Gene position: 2325278-2324445 (Counterclockwise)

Preceding gene: 52786123

Following gene: 52786121

Centisome position: 55.07

GC content: 51.08

Gene sequence:

>834_bases
ATGAAGACAAAAACTGACTTTCTAAAAATGAAACAAGAGGGAGAGCCGATCGTCATGCTGACTGCCTACGATTATCCGGC
GGCAAAGCTTGCCGAACAGGCCGGCGTCGATATGATCCTTGTCGGCGATTCACTCGGCATGGTTGTACTCGGCCTTGACT
CGACCGTATCTGTTACGGTTGCTGATATGATCCACCATACAAAAGCTGTCAAACGCGGGGCAAAAGATACGTTTGTTGTG
ACGGATATGCCTTTTATGTCATATCACTGCTCTTTGGAAAGCGCTCTGAAAAATGCCGCGGCGATCGTTCAAGAAAGCGG
GGCAGATGCTTTGAAGCTTGAAGGCGGCGACGGTGTGTTTGAGACGATCACGGCTTTAACACGCGGCGGAATACCTGTTG
TCAGCCATTTAGGCTTGACTCCTCAATCCGTCGGTGTTTTGGGAGGCTATAAAGTTCAAGGGAAAGATGAAGAAAGCGCC
GGCAAGCTGATTGAAGACAGCAGGCGCTGTGAAGAAGCAGGCGCGATGGCGCTCGTTCTGGAATGCGTCCCGGCAGCGCT
CGCTGAACGGATTTCAAAGGAGCTCACAATTCCTGTCATCGGCATTGGAGCAGGTGCTGGCACGGACGGACAGGTTCTCG
TTTACCACGATGTTGTCGGACACGGAGTAAGCCGGACGCCTAAATTTGTCAAGCAGTATGCACAAATCGACCAGCCGCTT
GAACAAGCGCTTCGGGGATATGTTGAGGATGTCAGAAGCCGCGTGTTTCCTGAAGATGCTCATTCGTTCCGTATAGATCA
GCAAGTTCTTGAAGGATTATACGGAGGGAAATAG

Upstream 100 bases:

>100_bases
TACGAAATCCTTCTCCTTGCCGGAAGGTTTTTTTGCTGTCCGAAAAGATGCAGATGCAAGCACGGCAGCTCATCTCCTCT
GAAAAAGAGGAGGAGAAAAC

Downstream 100 bases:

>100_bases
GCATGATTCAGATCTATACAGCAAAAGATATACAAAACTTAACGAAAAATTACCGCAAGGAAGGCAAAACCATCGGTTTT
GTGCCGACAATGGGCTTCCT

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MKTKTDFLKMKQEGEPIVMLTAYDYPAAKLAEQAGVDMILVGDSLGMVVLGLDSTVSVTVADMIHHTKAVKRGAKDTFVV
TDMPFMSYHCSLESALKNAAAIVQESGADALKLEGGDGVFETITALTRGGIPVVSHLGLTPQSVGVLGGYKVQGKDEESA
GKLIEDSRRCEEAGAMALVLECVPAALAERISKELTIPVIGIGAGAGTDGQVLVYHDVVGHGVSRTPKFVKQYAQIDQPL
EQALRGYVEDVRSRVFPEDAHSFRIDQQVLEGLYGGK

Sequences:

>Translated_277_residues
MKTKTDFLKMKQEGEPIVMLTAYDYPAAKLAEQAGVDMILVGDSLGMVVLGLDSTVSVTVADMIHHTKAVKRGAKDTFVV
TDMPFMSYHCSLESALKNAAAIVQESGADALKLEGGDGVFETITALTRGGIPVVSHLGLTPQSVGVLGGYKVQGKDEESA
GKLIEDSRRCEEAGAMALVLECVPAALAERISKELTIPVIGIGAGAGTDGQVLVYHDVVGHGVSRTPKFVKQYAQIDQPL
EQALRGYVEDVRSRVFPEDAHSFRIDQQVLEGLYGGK
>Mature_277_residues
MKTKTDFLKMKQEGEPIVMLTAYDYPAAKLAEQAGVDMILVGDSLGMVVLGLDSTVSVTVADMIHHTKAVKRGAKDTFVV
TDMPFMSYHCSLESALKNAAAIVQESGADALKLEGGDGVFETITALTRGGIPVVSHLGLTPQSVGVLGGYKVQGKDEESA
GKLIEDSRRCEEAGAMALVLECVPAALAERISKELTIPVIGIGAGAGTDGQVLVYHDVVGHGVSRTPKFVKQYAQIDQPL
EQALRGYVEDVRSRVFPEDAHSFRIDQQVLEGLYGGK

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=256, Percent_Identity=49.609375, Blast_Score=247, Evalue=6e-67,
Organism=Saccharomyces cerevisiae, GI6319653, Length=266, Percent_Identity=39.0977443609023, Blast_Score=196, Evalue=4e-51,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_BACLD (Q65I56)

Other databases:

- EMBL:   CP000002
- EMBL:   AE017333
- RefSeq:   YP_079542.1
- RefSeq:   YP_091951.1
- HSSP:   P31057
- ProteinModelPortal:   Q65I56
- SMR:   Q65I56
- STRING:   Q65I56
- EnsemblBacteria:   EBBACT00000056450
- EnsemblBacteria:   EBBACT00000062437
- GeneID:   3027472
- GeneID:   3100431
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi02378
- KEGG:   bli:BL02752
- NMPDR:   fig|279010.5.peg.228
- eggNOG:   COG0413
- GeneTree:   EBGT00050000000505
- HOGENOM:   HBG299908
- OMA:   YATPEQT
- ProtClustDB:   PRK00311
- BioCyc:   BLIC279010-1:BLI02378-MONOMER
- BioCyc:   BLIC279010:BL02752-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 29519; Mature: 29519

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: ACT_SITE 181-181 BINDING 82-82 BINDING 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTKTDFLKMKQEGEPIVMLTAYDYPAAKLAEQAGVDMILVGDSLGMVVLGLDSTVSVTV
CCCHHHHHHHHCCCCCEEEEEECCCCHHHHHHHCCCCEEEECCCCCEEEEECCCCHHHHH
ADMIHHTKAVKRGAKDTFVVTDMPFMSYHCSLESALKNAAAIVQESGADALKLEGGDGVF
HHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHH
ETITALTRGGIPVVSHLGLTPQSVGVLGGYKVQGKDEESAGKLIEDSRRCEEAGAMALVL
HHHHHHHCCCCHHHHHCCCCHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
ECVPAALAERISKELTIPVIGIGAGAGTDGQVLVYHDVVGHGVSRTPKFVKQYAQIDQPL
HHHHHHHHHHHHHCCEEEEEEECCCCCCCCCEEEEECHHCCCCCCCHHHHHHHHHHHHHH
EQALRGYVEDVRSRVFPEDAHSFRIDQQVLEGLYGGK
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKTKTDFLKMKQEGEPIVMLTAYDYPAAKLAEQAGVDMILVGDSLGMVVLGLDSTVSVTV
CCCHHHHHHHHCCCCCEEEEEECCCCHHHHHHHCCCCEEEECCCCCEEEEECCCCHHHHH
ADMIHHTKAVKRGAKDTFVVTDMPFMSYHCSLESALKNAAAIVQESGADALKLEGGDGVF
HHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHH
ETITALTRGGIPVVSHLGLTPQSVGVLGGYKVQGKDEESAGKLIEDSRRCEEAGAMALVL
HHHHHHHCCCCHHHHHCCCCHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
ECVPAALAERISKELTIPVIGIGAGAGTDGQVLVYHDVVGHGVSRTPKFVKQYAQIDQPL
HHHHHHHHHHHHHCCEEEEEEECCCCCCCCCEEEEECHHCCCCCCCHHHHHHHHHHHHHH
EQALRGYVEDVRSRVFPEDAHSFRIDQQVLEGLYGGK
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA