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The map label for this gene is ypqE [H]

Identifier: 52786103

GI number: 52786103

Start: 2307501

End: 2308007

Strand: Reverse

Name: ypqE [H]

Synonym: BLi02359

Alternate gene names: 52786103

Gene position: 2308007-2307501 (Counterclockwise)

Preceding gene: 52786105

Following gene: 52786102

Centisome position: 54.66

GC content: 46.15

Gene sequence:

>507_bases
TTGCTGAAAAAATTATTCGGTATTGGTCAAAAACAAGATGAAGTGAAAGAAGAAACCGTTTTTTCACCGGTTGACGGACA
AGTTTTGGAACTTTCAAAAGTTCCTGACCCCGTTTTCTCTCAAAAGATGATGGGTGACGGAATGGCTGTCGAGCCTTCCG
CGGGCGAGATCGTATCGCCTGTCGACGGGGAAGTGATCCAGCTGTTCCACACAAAACACGCCATAGGCATTCGTTCATTG
TCCGGCGTGGAGCTCTTGATTCACATCGGTCTGGAAACCGTAGCAATGGAAGGCGAAGGTTTCACAGCCCATGTCAAAGA
AGGAGACAAAGTAAAAGTCGGCGATCCTTTAATAACATGCGATTTGGAACTGATCAAGGAAAAAGCGAGCAGCACAATTA
CGCCGATCGTCATTATGAACGGCGATGTGTTGGAACGCGTCACAAAACATGAAGAGGCAGCAGCGAAAAAAAGCGAGACA
AAGCTCATGGAGCTTAAAGTAAAGTAA

Upstream 100 bases:

>100_bases
AAAGTGTGGTTAAATAGTGGGGAATCCAACGATTTGGTATTTAACCTGTTCCTCTTTTAATAGCCATCATAAAGAGGCGT
TTTGAAAGGAGCCCACTGTA

Downstream 100 bases:

>100_bases
AGTAAAAGAAAAAAGCAGAGAGCGCTTCTCTGCTTTTTCATTTTGTCAGGAAATCACCTCTTTCAGAACGAACGGACATT
CGCTAAAATAGAAGGGAGAG

Product: YpqE

Products: protein histidine; sugar phosphate

Alternate protein names: Putative PTS system EIIA component [H]

Number of amino acids: Translated: 168; Mature: 168

Protein sequence:

>168_residues
MLKKLFGIGQKQDEVKEETVFSPVDGQVLELSKVPDPVFSQKMMGDGMAVEPSAGEIVSPVDGEVIQLFHTKHAIGIRSL
SGVELLIHIGLETVAMEGEGFTAHVKEGDKVKVGDPLITCDLELIKEKASSTITPIVIMNGDVLERVTKHEEAAAKKSET
KLMELKVK

Sequences:

>Translated_168_residues
MLKKLFGIGQKQDEVKEETVFSPVDGQVLELSKVPDPVFSQKMMGDGMAVEPSAGEIVSPVDGEVIQLFHTKHAIGIRSL
SGVELLIHIGLETVAMEGEGFTAHVKEGDKVKVGDPLITCDLELIKEKASSTITPIVIMNGDVLERVTKHEEAAAKKSET
KLMELKVK
>Mature_168_residues
MLKKLFGIGQKQDEVKEETVFSPVDGQVLELSKVPDPVFSQKMMGDGMAVEPSAGEIVSPVDGEVIQLFHTKHAIGIRSL
SGVELLIHIGLETVAMEGEGFTAHVKEGDKVKVGDPLITCDLELIKEKASSTITPIVIMNGDVLERVTKHEEAAAKKSET
KLMELKVK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane [H]

COG id: COG2190

COG function: function code G; Phosphotransferase system IIA components

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788757, Length=130, Percent_Identity=46.1538461538462, Blast_Score=131, Evalue=2e-32,
Organism=Escherichia coli, GI1790159, Length=143, Percent_Identity=43.3566433566434, Blast_Score=123, Evalue=5e-30,
Organism=Escherichia coli, GI1786894, Length=145, Percent_Identity=37.2413793103448, Blast_Score=116, Evalue=8e-28,

Paralogues:

None

Copy number: 3540 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR001127 [H]

Pfam domain/function: PF00358 PTS_EIIA_1 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 18219; Mature: 18219

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00371 PTS_EIIA_TYPE_1_HIS ; PS51093 PTS_EIIA_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKLFGIGQKQDEVKEETVFSPVDGQVLELSKVPDPVFSQKMMGDGMAVEPSAGEIVSP
CCCHHHCCCCCHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHCCCEEECCCCCCEECC
VDGEVIQLFHTKHAIGIRSLSGVELLIHIGLETVAMEGEGFTAHVKEGDKVKVGDPLITC
CCCHHHHHHHHHHHHCCCCCCCEEEEEEECCEEEEECCCCCEEECCCCCEEEECCCEEEE
DLELIKEKASSTITPIVIMNGDVLERVTKHEEAAAKKSETKLMELKVK
CHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEEEC
>Mature Secondary Structure
MLKKLFGIGQKQDEVKEETVFSPVDGQVLELSKVPDPVFSQKMMGDGMAVEPSAGEIVSP
CCCHHHCCCCCHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHCCCEEECCCCCCEECC
VDGEVIQLFHTKHAIGIRSLSGVELLIHIGLETVAMEGEGFTAHVKEGDKVKVGDPLITC
CCCHHHHHHHHHHHHCCCCCCCEEEEEEECCEEEEECCCCCEEECCCCCEEEECCCEEEE
DLELIKEKASSTITPIVIMNGDVLERVTKHEEAAAKKSETKLMELKVK
CHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8760912; 9384377 [H]