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The map label for this gene is ilvD [H]

Identifier: 52786068

GI number: 52786068

Start: 2273303

End: 2274979

Strand: Reverse

Name: ilvD [H]

Synonym: BLi02324

Alternate gene names: 52786068

Gene position: 2274979-2273303 (Counterclockwise)

Preceding gene: 52786069

Following gene: 52786067

Centisome position: 53.88

GC content: 52.59

Gene sequence:

>1677_bases
ATGACAGGTTTACGCAGTGACATGATTACAAAAGGGATCGACAGAGCGCCGCACCGCAGTTTGCTGCGCGCGGCTGGGGT
AAAAGAAGAGGACTTCGGCAAACCGTTTATTGCCGTTTGCAACTCATACATCGATATCGTACCGGGTCATGTCCATTTGC
AGGAGTTTGGAAAAATCGTCAAAGAGGCGATCAGAGAGGCCGGCGGTGTTCCGTTTGAATTTAATACAATCGGGGTCGAC
GACGGAATTGCGATGGGGCACATCGGAATGAGGTATTCTCTCCCGAGCCGCGAAATCATCGCAGATTCAGTGGAAACGGT
TGTATCGGCGCACTGGTTTGACGGAATGGTATGTATTCCAAACTGTGATAAAATCACACCGGGCATGATCATGGCGGCAA
TGCGGATCAACATTCCGACCGTGTTTGTCAGCGGGGGGCCGATGGAAGCGGGAAGAACGAGCGACGGACGAAAAATCTCG
CTTTCCTCTGTATTTGAAGGCGTTGGCGCTTATCAATCAGGCAAAATCGATGAGAAAGGACTCGAGGAGCTTGAACAGTT
CGGCTGTCCGACTTGCGGATCATGCTCGGGCATGTTTACGGCGAACTCGATGAACTGTCTTTCTGAAGCTCTTGGCATCG
CCATGCCGGGCAACGGCACCATTTTGGCGACATCGCCCGACCGCAGGGAATTTGCCAAACAGTCGGCCCGCCAGCTGATG
GAGCTGATCAAGTCGGATATCAAACCGCGCGACATCGTGACCGAAAAAGCGATCGACAACGCGTTCGCTTTAGACATGGC
GCTCGGCGGATCAACGAATACGATCCTTCATACGCTTGCGATCGCCAATGAAGCGGGTGTAGACTATTCGCTTGAACGGA
TCAATGAGGTAGCGGCAAGGGTTCCGCATTTATCGAAGCTTGCACCGGCTTCCGATGTGTTTATTGAAGATTTGCATGAA
GCAGGAGGCGTATCGGCAGTCTTAAACGAGCTGTCGAAAAAAGAAGGCGCGCTTCACTTGGATACGCTGACTGTAACGGG
GAAAACGCTTGGCGAAAATATTGCCGGACGCGAAGTGAAAGATTACGAGGTCATTCATCCGATCGATCAGCCGTTTTCAG
AGCAAGGCGGACTCGCCGTCCTGTTCGGCAACCTGGCTCCTGACGGTGCGATCATTAAAACGGGCGGCGTCCAAGACGGG
ATTACCCGCCATGAAGGACCTGCGGTTGTCTTTGATTCACAGGAAGAAGCGCTTGACGGCATCATCAACCGTAAAGTAAA
AGCGGGAGATGTCGTCATCATCCGCTATGAAGGCCCTAAAGGCGGACCGGGAATGCCTGAAATGCTTGCGCCGACTTCAC
AGATCGTCGGAATGGGCCTCGGCCCGAAAGTCGCCTTGATTACCGACGGCCGCTTTTCAGGAGCCTCCCGCGGTCTTTCG
ATCGGCCACGTTTCACCGGAAGCAGCCGAAGGCGGCCCGCTTGCTTTCGTAGAAAACGGCGACCATATCGTTGTCGATAT
CGAAAAGCGGATTTTAAACATCGAAATCTCCGATGAGGAATGGGAAAAAAGAAAAGCAAACTGGCCCGGCTTTGAACCGA
AAGTGAAAACGGGCTATCTCGCCAGGTATTCAAAGCTTGTGACATCTGCCAATACCGGCGGCATTATGAAAATCTAG

Upstream 100 bases:

>100_bases
ATCTTGCGGATCAGTCCGGCAAGCGGTTAACAGACAATTTTTAAAACTGAATATTCAGAATATATAGAATGTAAATACGA
AGATGTTTAGGAGGATCACG

Downstream 100 bases:

>100_bases
CGGTTTTTTCGCCCGGCGTTTGAAATTCAGCCCCCTCTGTAGTATCCTTGAAAACATCAGTAGTCAAGGTTTATAGAGGG
GGCTTTTTAATATGTCAACA

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD [H]

Number of amino acids: Translated: 558; Mature: 557

Protein sequence:

>558_residues
MTGLRSDMITKGIDRAPHRSLLRAAGVKEEDFGKPFIAVCNSYIDIVPGHVHLQEFGKIVKEAIREAGGVPFEFNTIGVD
DGIAMGHIGMRYSLPSREIIADSVETVVSAHWFDGMVCIPNCDKITPGMIMAAMRINIPTVFVSGGPMEAGRTSDGRKIS
LSSVFEGVGAYQSGKIDEKGLEELEQFGCPTCGSCSGMFTANSMNCLSEALGIAMPGNGTILATSPDRREFAKQSARQLM
ELIKSDIKPRDIVTEKAIDNAFALDMALGGSTNTILHTLAIANEAGVDYSLERINEVAARVPHLSKLAPASDVFIEDLHE
AGGVSAVLNELSKKEGALHLDTLTVTGKTLGENIAGREVKDYEVIHPIDQPFSEQGGLAVLFGNLAPDGAIIKTGGVQDG
ITRHEGPAVVFDSQEEALDGIINRKVKAGDVVIIRYEGPKGGPGMPEMLAPTSQIVGMGLGPKVALITDGRFSGASRGLS
IGHVSPEAAEGGPLAFVENGDHIVVDIEKRILNIEISDEEWEKRKANWPGFEPKVKTGYLARYSKLVTSANTGGIMKI

Sequences:

>Translated_558_residues
MTGLRSDMITKGIDRAPHRSLLRAAGVKEEDFGKPFIAVCNSYIDIVPGHVHLQEFGKIVKEAIREAGGVPFEFNTIGVD
DGIAMGHIGMRYSLPSREIIADSVETVVSAHWFDGMVCIPNCDKITPGMIMAAMRINIPTVFVSGGPMEAGRTSDGRKIS
LSSVFEGVGAYQSGKIDEKGLEELEQFGCPTCGSCSGMFTANSMNCLSEALGIAMPGNGTILATSPDRREFAKQSARQLM
ELIKSDIKPRDIVTEKAIDNAFALDMALGGSTNTILHTLAIANEAGVDYSLERINEVAARVPHLSKLAPASDVFIEDLHE
AGGVSAVLNELSKKEGALHLDTLTVTGKTLGENIAGREVKDYEVIHPIDQPFSEQGGLAVLFGNLAPDGAIIKTGGVQDG
ITRHEGPAVVFDSQEEALDGIINRKVKAGDVVIIRYEGPKGGPGMPEMLAPTSQIVGMGLGPKVALITDGRFSGASRGLS
IGHVSPEAAEGGPLAFVENGDHIVVDIEKRILNIEISDEEWEKRKANWPGFEPKVKTGYLARYSKLVTSANTGGIMKI
>Mature_557_residues
TGLRSDMITKGIDRAPHRSLLRAAGVKEEDFGKPFIAVCNSYIDIVPGHVHLQEFGKIVKEAIREAGGVPFEFNTIGVDD
GIAMGHIGMRYSLPSREIIADSVETVVSAHWFDGMVCIPNCDKITPGMIMAAMRINIPTVFVSGGPMEAGRTSDGRKISL
SSVFEGVGAYQSGKIDEKGLEELEQFGCPTCGSCSGMFTANSMNCLSEALGIAMPGNGTILATSPDRREFAKQSARQLME
LIKSDIKPRDIVTEKAIDNAFALDMALGGSTNTILHTLAIANEAGVDYSLERINEVAARVPHLSKLAPASDVFIEDLHEA
GGVSAVLNELSKKEGALHLDTLTVTGKTLGENIAGREVKDYEVIHPIDQPFSEQGGLAVLFGNLAPDGAIIKTGGVQDGI
TRHEGPAVVFDSQEEALDGIINRKVKAGDVVIIRYEGPKGGPGMPEMLAPTSQIVGMGLGPKVALITDGRFSGASRGLSI
GHVSPEAAEGGPLAFVENGDHIVVDIEKRILNIEISDEEWEKRKANWPGFEPKVKTGYLARYSKLVTSANTGGIMKI

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family [H]

Homologues:

Organism=Escherichia coli, GI48994964, Length=609, Percent_Identity=47.4548440065681, Blast_Score=545, Evalue=1e-156,
Organism=Escherichia coli, GI1788157, Length=558, Percent_Identity=33.3333333333333, Blast_Score=232, Evalue=4e-62,
Organism=Escherichia coli, GI2367371, Length=505, Percent_Identity=33.8613861386139, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1786464, Length=504, Percent_Identity=32.3412698412698, Blast_Score=203, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6322476, Length=560, Percent_Identity=39.1071428571429, Blast_Score=403, Evalue=1e-113,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558 [H]

Pfam domain/function: PF00920 ILVD_EDD [H]

EC number: =4.2.1.9 [H]

Molecular weight: Translated: 59462; Mature: 59331

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00886 ILVD_EDD_1 ; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGLRSDMITKGIDRAPHRSLLRAAGVKEEDFGKPFIAVCNSYIDIVPGHVHLQEFGKIV
CCCCHHHHHHHCCCCCHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHCCCEEEHHHHHHHH
KEAIREAGGVPFEFNTIGVDDGIAMGHIGMRYSLPSREIIADSVETVVSAHWFDGMVCIP
HHHHHHHCCCCEEEEEECCCCCCEECCCCEEECCCCHHHHHHHHHHHHHHHHCCCEEECC
NCDKITPGMIMAAMRINIPTVFVSGGPMEAGRTSDGRKISLSSVFEGVGAYQSGKIDEKG
CCCCCCHHHHHHHEEECCCEEEEECCCCCCCCCCCCCEEEHHHHHHHHCCCCCCCCCHHH
LEELEQFGCPTCGSCSGMFTANSMNCLSEALGIAMPGNGTILATSPDRREFAKQSARQLM
HHHHHHCCCCCCCCCCCCEECCHHHHHHHHHCEEECCCCEEEECCCCHHHHHHHHHHHHH
ELIKSDIKPRDIVTEKAIDNAFALDMALGGSTNTILHTLAIANEAGVDYSLERINEVAAR
HHHHHCCCCHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHH
VPHLSKLAPASDVFIEDLHEAGGVSAVLNELSKKEGALHLDTLTVTGKTLGENIAGREVK
CCCHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEEEEECHHHCCCCCCCCCC
DYEVIHPIDQPFSEQGGLAVLFGNLAPDGAIIKTGGVQDGITRHEGPAVVFDSQEEALDG
CCEEECCCCCCCCCCCCEEEEECCCCCCCCEEECCCCCCCCCCCCCCEEEECCHHHHHHH
IINRKVKAGDVVIIRYEGPKGGPGMPEMLAPTSQIVGMGLGPKVALITDGRFSGASRGLS
HHHCCEECCCEEEEEECCCCCCCCCCHHHCCHHHHHCCCCCCEEEEEECCCCCCCCCCCE
IGHVSPEAAEGGPLAFVENGDHIVVDIEKRILNIEISDEEWEKRKANWPGFEPKVKTGYL
ECCCCCCCCCCCCEEEEECCCEEEEEEEEEEEEEEECCHHHHHHCCCCCCCCCCCCHHHH
ARYSKLVTSANTGGIMKI
HHHHHHHHCCCCCCEEEC
>Mature Secondary Structure 
TGLRSDMITKGIDRAPHRSLLRAAGVKEEDFGKPFIAVCNSYIDIVPGHVHLQEFGKIV
CCCHHHHHHHCCCCCHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHCCCEEEHHHHHHHH
KEAIREAGGVPFEFNTIGVDDGIAMGHIGMRYSLPSREIIADSVETVVSAHWFDGMVCIP
HHHHHHHCCCCEEEEEECCCCCCEECCCCEEECCCCHHHHHHHHHHHHHHHHCCCEEECC
NCDKITPGMIMAAMRINIPTVFVSGGPMEAGRTSDGRKISLSSVFEGVGAYQSGKIDEKG
CCCCCCHHHHHHHEEECCCEEEEECCCCCCCCCCCCCEEEHHHHHHHHCCCCCCCCCHHH
LEELEQFGCPTCGSCSGMFTANSMNCLSEALGIAMPGNGTILATSPDRREFAKQSARQLM
HHHHHHCCCCCCCCCCCCEECCHHHHHHHHHCEEECCCCEEEECCCCHHHHHHHHHHHHH
ELIKSDIKPRDIVTEKAIDNAFALDMALGGSTNTILHTLAIANEAGVDYSLERINEVAAR
HHHHHCCCCHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHH
VPHLSKLAPASDVFIEDLHEAGGVSAVLNELSKKEGALHLDTLTVTGKTLGENIAGREVK
CCCHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEEEEECHHHCCCCCCCCCC
DYEVIHPIDQPFSEQGGLAVLFGNLAPDGAIIKTGGVQDGITRHEGPAVVFDSQEEALDG
CCEEECCCCCCCCCCCCEEEEECCCCCCCCEEECCCCCCCCCCCCCCEEEECCHHHHHHH
IINRKVKAGDVVIIRYEGPKGGPGMPEMLAPTSQIVGMGLGPKVALITDGRFSGASRGLS
HHHCCEECCCEEEEEECCCCCCCCCCHHHCCHHHHHCCCCCCEEEEEECCCCCCCCCCCE
IGHVSPEAAEGGPLAFVENGDHIVVDIEKRILNIEISDEEWEKRKANWPGFEPKVKTGYL
ECCCCCCCCCCCCEEEEECCCEEEEEEEEEEEEEEECCHHHHHHCCCCCCCCCCCCHHHH
ARYSKLVTSANTGGIMKI
HHHHHHHHCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA