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The map label for this gene is ydfA [H]

Identifier: 52785875

GI number: 52785875

Start: 2069508

End: 2070803

Strand: Reverse

Name: ydfA [H]

Synonym: BLi02123

Alternate gene names: 52785875

Gene position: 2070803-2069508 (Counterclockwise)

Preceding gene: 52785876

Following gene: 52785874

Centisome position: 49.04

GC content: 47.69

Gene sequence:

>1296_bases
ATGACGACAGTCTTAGCCACTTTGATTTTTCTGCTGACGCTCACGCTCGTGATTTGGCAGCCGAAAAATCTATCCATCGG
CTGGTCGGCCTGCGGAGGAGCGGTGCTGGCTTTAATTGCCGGAGTCGTCAATTTTCAGGATGTATTAGACGTAACGGGAA
TCGTATGGAATGCGACTTTGGCATTTGTTGCGATCATCATTATCTCCCTGATCCTTGATGAAATCGGATTTTTCGAGTGG
GCCGCCCTGCACATGGCCAAGGCAGCTAAAGGAAACGGCATTCGGATGTTTGTCTATGTCACCCTGCTGGGAGCCGTAGT
AGCCGCGCTGTTTGCCAATGACGGAGCTGCTTTAATTTTGACGCCGATTGTGCTTGCCATGGTACGCGCGTTAAATTTTC
AAGAAAAGCTCGTTTTTCCTTTTATTATCGCAAGCGGATTTATCGCGGATACAACATCGCTGCCGCTTGTTGTCAGCAAC
CTTGTCAATATTGTCTCAGCAGACTTTTTCAACATCGGATTTATCGAATATGCAACAAGAATGATTGTTCCAAACCTGTT
TTCGCTTATTGCAAGCATCATCGTGCTTTTTTTGTTTTTCAGAAAAAGCATTCCGCGGCATTATGACATGACAAATGTAA
AACAGCCAGCGGAAGCGATTAGAGACAGGAAAATATTCCGCTTGTCATGGCTCATATTAGCCGTTCTGTTGGCCGGTTAC
TTTATCAGCGAGCTGTTCTCGATCCCTGTCTCGATCATCGCGGGCGTGATCGCCGTCATTTTCTTAATTACAGCGAAAAA
CAGCCCCGCGGTGCAAACGAAGAAAGTGCTGAAGGGAGCGCCTTGGGCGATCGTTTTCTTCTCAATCGGGATGTATGTCG
TGGTATACGGTTTGAGAAACGCCGGCTTAACAGATGTTCTCTCACAGGTCATCCAGACCGTCAGCGATCAAGGGCTGTTT
GCCGGAACAATGGGAATGGGCTTTATCGCAGCCATCCTGTCTTCATTAATGAACAATATGCCGACGGTGATGATTGATGC
GCTTGCGATTCAGAATACCGATACACACGGGATTCTCAGAGAAGCGCTGATTTACGCCAATGTCATCGGCTCTGATCTCG
GTCCGAAAATCACGCCGATCGGCTCCCTGGCGACGCTCTTGTGGCTTCACGTCCTTTCACTGAAAGGCGTAAAGATTTCT
TGGGGAACCTATTTTAAAACAGGTGTCATTTTGACCATTCCAACCCTGTTCATCACGCTTGCCGGACTTTATGTGTGGCT
GCTGATCATCCATTAA

Upstream 100 bases:

>100_bases
CTCTTTTTCATTTGCATTCCGAATATAAGCATTGACTTATATAACAATTTTATTATAAGATGTGTCCGGAGAGGAAACCT
ATGAAGGAGGCGTACACGCG

Downstream 100 bases:

>100_bases
ACATGATAGGAGACGATGACCAATGTCAAAAAAGACCATCTATTTTTTATGCACAGGCAACTCCTGCCGCAGCCAAATGG
CTGAAGGCTGGGCGAAGAAA

Product: YdfA

Products: ADP; phosphate; arsenite [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MTTVLATLIFLLTLTLVIWQPKNLSIGWSACGGAVLALIAGVVNFQDVLDVTGIVWNATLAFVAIIIISLILDEIGFFEW
AALHMAKAAKGNGIRMFVYVTLLGAVVAALFANDGAALILTPIVLAMVRALNFQEKLVFPFIIASGFIADTTSLPLVVSN
LVNIVSADFFNIGFIEYATRMIVPNLFSLIASIIVLFLFFRKSIPRHYDMTNVKQPAEAIRDRKIFRLSWLILAVLLAGY
FISELFSIPVSIIAGVIAVIFLITAKNSPAVQTKKVLKGAPWAIVFFSIGMYVVVYGLRNAGLTDVLSQVIQTVSDQGLF
AGTMGMGFIAAILSSLMNNMPTVMIDALAIQNTDTHGILREALIYANVIGSDLGPKITPIGSLATLLWLHVLSLKGVKIS
WGTYFKTGVILTIPTLFITLAGLYVWLLIIH

Sequences:

>Translated_431_residues
MTTVLATLIFLLTLTLVIWQPKNLSIGWSACGGAVLALIAGVVNFQDVLDVTGIVWNATLAFVAIIIISLILDEIGFFEW
AALHMAKAAKGNGIRMFVYVTLLGAVVAALFANDGAALILTPIVLAMVRALNFQEKLVFPFIIASGFIADTTSLPLVVSN
LVNIVSADFFNIGFIEYATRMIVPNLFSLIASIIVLFLFFRKSIPRHYDMTNVKQPAEAIRDRKIFRLSWLILAVLLAGY
FISELFSIPVSIIAGVIAVIFLITAKNSPAVQTKKVLKGAPWAIVFFSIGMYVVVYGLRNAGLTDVLSQVIQTVSDQGLF
AGTMGMGFIAAILSSLMNNMPTVMIDALAIQNTDTHGILREALIYANVIGSDLGPKITPIGSLATLLWLHVLSLKGVKIS
WGTYFKTGVILTIPTLFITLAGLYVWLLIIH
>Mature_430_residues
TTVLATLIFLLTLTLVIWQPKNLSIGWSACGGAVLALIAGVVNFQDVLDVTGIVWNATLAFVAIIIISLILDEIGFFEWA
ALHMAKAAKGNGIRMFVYVTLLGAVVAALFANDGAALILTPIVLAMVRALNFQEKLVFPFIIASGFIADTTSLPLVVSNL
VNIVSADFFNIGFIEYATRMIVPNLFSLIASIIVLFLFFRKSIPRHYDMTNVKQPAEAIRDRKIFRLSWLILAVLLAGYF
ISELFSIPVSIIAGVIAVIFLITAKNSPAVQTKKVLKGAPWAIVFFSIGMYVVVYGLRNAGLTDVLSQVIQTVSDQGLFA
GTMGMGFIAAILSSLMNNMPTVMIDALAIQNTDTHGILREALIYANVIGSDLGPKITPIGSLATLLWLHVLSLKGVKISW
GTYFKTGVILTIPTLFITLAGLYVWLLIIH

Specific function: Involved In Arsenical Resistance. Thought To Form The Channel Of An Arsenite Pump (By Similarity). [C]

COG id: COG1055

COG function: function code P; Na+/H+ antiporter NhaD and related arsenite permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ArsB family [H]

Homologues:

Organism=Escherichia coli, GI87082277, Length=420, Percent_Identity=60.7142857142857, Blast_Score=498, Evalue=1e-142,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000802 [H]

Pfam domain/function: PF02040 ArsB [H]

EC number: NA

Molecular weight: Translated: 46724; Mature: 46593

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHCC
>Mature Secondary Structure 
TTVLATLIFLLTLTLVIWQPKNLSIGWSACGGAVLALIAGVVNFQDVLDVTGIVWNATL
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH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HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; arsenite [Cytoplasm]; H2O [C]

Specific reaction: ATP + arsenite [Cytoplasm] + H2O = ADP + phosphate + arsenite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]