BacMap

Definition	Bacillus licheniformis ATCC 14580, complete genome.
Accession	NC_006322
Length	4,222,645

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The map label for this gene is uxuB [H]

Identifier: 52785867

GI number: 52785867

Start: 2059844

End: 2060695

Strand: Reverse

Name: uxuB [H]

Synonym: BLi02115

Alternate gene names: 52785867

Gene position: 2060695-2059844 (Counterclockwise)

Preceding gene: 52785868

Following gene: 52785866

Centisome position: 48.8

GC content: 43.78

Gene sequence:

>852_bases
GTGCAGATTCCTTTTCATGTCAACTTGAAAGATAAGGTGGCTGTAGTGACAGGTGGAAGCGGCATCTTATGCAGCGGTTT
CGCTAAAGCATTAGCTCAATGCGGTGCAAAAGTAGCAGTAGTCAGCCGAAGAGCTGAATCGGCAGACAGAGTAGCGAGAG
ATATCCAAGAAAACGGAGGCGAAGCACTTGCCGTTACAGCAAACGTCCTTGATAAACAAAGCCTTGAACAAGCGAAACAA
CTCATTAACAGAACATTCGGCAGATGCAATATCCTCATTAACGGAGCAGGCGGCAATCATCCAATGGGAACAACAGATAA
AGAATATTTATATAAAAAAGACTTGAATACTAAAGAATTGACTACGTTTTTTGATTTAGAAATAGAGGGTGTAAGGTCTG
TATTGGATTTAAACTTTCTTGGTACACTGTTAACCACTCAAGTATTTGCAAAAGACATGGCTGATCACGACGGAAATGTG
ATCGTTAACATCTCATCCATGAACGCCTATACGCCGCTGACGAAAATTCCCGCCTACAGCGGTGCAAAAGCGGCCGTCAG
TAACTTTACACAATGGCTTGCTGTGCATATGTCAAAAGTCGGAATTCGTGTCAATGCCATTGCCCCAGGCTTTTTTCTTA
CCGCCCAAAATAGGAGCCTATTGCTCAATGAAGACGGAACATATACGGAGCGAGCACAAAAAATTATCAACCAGACGCCG
TTGGATAGATTTGGGGTTCCGGAAGATCTAATCGGAACGTTATTATGGTTGGTAAGCGATAAAGCCTCGCGATTTGTCAC
TGGAACCATCATACCGGTCGATGGAGGATTCTCCGCCTATTCTGGAGTATAA

Upstream 100 bases:

>100_bases
AATGGAATGTGGGAAACATTGACGAAAGAAAAGAAGCGGATAGCCTTAGAATGCAATAAATGAACAAGCACTAACTAAAA
TGCAGAGACGAGGGATAAAT

Downstream 100 bases:

>100_bases
CTAACGGGGTTAGCTCCAAAAAGCTGATTTTCAACACTGATAAAAATCCCAATTTCTCTGTGTCACATTGAGAAATTGGG
ATTTATTATTTCAACTTTTC

Product: D-mannonate oxidoreductase

Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MQIPFHVNLKDKVAVVTGGSGILCSGFAKALAQCGAKVAVVSRRAESADRVARDIQENGGEALAVTANVLDKQSLEQAKQ
LINRTFGRCNILINGAGGNHPMGTTDKEYLYKKDLNTKELTTFFDLEIEGVRSVLDLNFLGTLLTTQVFAKDMADHDGNV
IVNISSMNAYTPLTKIPAYSGAKAAVSNFTQWLAVHMSKVGIRVNAIAPGFFLTAQNRSLLLNEDGTYTERAQKIINQTP
LDRFGVPEDLIGTLLWLVSDKASRFVTGTIIPVDGGFSAYSGV

Sequences:

>Translated_283_residues
MQIPFHVNLKDKVAVVTGGSGILCSGFAKALAQCGAKVAVVSRRAESADRVARDIQENGGEALAVTANVLDKQSLEQAKQ
LINRTFGRCNILINGAGGNHPMGTTDKEYLYKKDLNTKELTTFFDLEIEGVRSVLDLNFLGTLLTTQVFAKDMADHDGNV
IVNISSMNAYTPLTKIPAYSGAKAAVSNFTQWLAVHMSKVGIRVNAIAPGFFLTAQNRSLLLNEDGTYTERAQKIINQTP
LDRFGVPEDLIGTLLWLVSDKASRFVTGTIIPVDGGFSAYSGV
>Mature_283_residues
MQIPFHVNLKDKVAVVTGGSGILCSGFAKALAQCGAKVAVVSRRAESADRVARDIQENGGEALAVTANVLDKQSLEQAKQ
LINRTFGRCNILINGAGGNHPMGTTDKEYLYKKDLNTKELTTFFDLEIEGVRSVLDLNFLGTLLTTQVFAKDMADHDGNV
IVNISSMNAYTPLTKIPAYSGAKAAVSNFTQWLAVHMSKVGIRVNAIAPGFFLTAQNRSLLLNEDGTYTERAQKIINQTP
LDRFGVPEDLIGTLLWLVSDKASRFVTGTIIPVDGGFSAYSGV

Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI32483357, Length=271, Percent_Identity=28.7822878228782, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI40254992, Length=267, Percent_Identity=27.3408239700375, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI19923817, Length=281, Percent_Identity=28.8256227758007, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI31542939, Length=265, Percent_Identity=30.188679245283, Blast_Score=85, Evalue=9e-17,
Organism=Homo sapiens, GI5031737, Length=272, Percent_Identity=29.7794117647059, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI126723750, Length=271, Percent_Identity=26.1992619926199, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI10190704, Length=272, Percent_Identity=27.9411764705882, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI7705925, Length=274, Percent_Identity=25.5474452554745, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI4758504, Length=288, Percent_Identity=27.4305555555556, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI33667109, Length=209, Percent_Identity=29.6650717703349, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI59889578, Length=249, Percent_Identity=30.1204819277108, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI4503301, Length=271, Percent_Identity=26.5682656826568, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI7706318, Length=207, Percent_Identity=25.6038647342995, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI1787905, Length=271, Percent_Identity=30.2583025830258, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI1790717, Length=272, Percent_Identity=28.6764705882353, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1789208, Length=276, Percent_Identity=30.7971014492754, Blast_Score=104, Evalue=5e-24,
Organism=Escherichia coli, GI87082160, Length=270, Percent_Identity=27.4074074074074, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI1788459, Length=272, Percent_Identity=29.4117647058824, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI2367175, Length=270, Percent_Identity=29.6296296296296, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1789378, Length=271, Percent_Identity=27.6752767527675, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787335, Length=274, Percent_Identity=28.1021897810219, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI87082100, Length=275, Percent_Identity=27.6363636363636, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1789057, Length=275, Percent_Identity=27.6363636363636, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1786812, Length=273, Percent_Identity=27.1062271062271, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17538486, Length=278, Percent_Identity=32.3741007194245, Blast_Score=101, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17560676, Length=283, Percent_Identity=29.6819787985866, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17536025, Length=270, Percent_Identity=29.2592592592593, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17559104, Length=282, Percent_Identity=29.0780141843972, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17560332, Length=276, Percent_Identity=29.7101449275362, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17561402, Length=271, Percent_Identity=28.4132841328413, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17551412, Length=283, Percent_Identity=28.9752650176678, Blast_Score=87, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17536651, Length=270, Percent_Identity=28.8888888888889, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71994604, Length=269, Percent_Identity=28.6245353159851, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17565030, Length=282, Percent_Identity=30.1418439716312, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71994600, Length=270, Percent_Identity=29.2592592592593, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17562906, Length=282, Percent_Identity=26.5957446808511, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI115534694, Length=279, Percent_Identity=26.8817204301075, Blast_Score=85, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI72000259, Length=288, Percent_Identity=27.4305555555556, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17563726, Length=281, Percent_Identity=28.1138790035587, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17562910, Length=279, Percent_Identity=28.673835125448, Blast_Score=84, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17560150, Length=280, Percent_Identity=26.4285714285714, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17560220, Length=281, Percent_Identity=27.0462633451957, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17562904, Length=288, Percent_Identity=27.7777777777778, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17555706, Length=270, Percent_Identity=25.5555555555556, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17538182, Length=274, Percent_Identity=28.8321167883212, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI25147288, Length=275, Percent_Identity=27.2727272727273, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI193204405, Length=283, Percent_Identity=27.208480565371, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17561272, Length=272, Percent_Identity=29.0441176470588, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17531453, Length=275, Percent_Identity=26.5454545454545, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17538480, Length=281, Percent_Identity=28.4697508896797, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17562990, Length=283, Percent_Identity=25.7950530035336, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17562908, Length=274, Percent_Identity=25.5474452554745, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17544670, Length=277, Percent_Identity=25.2707581227437, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6324126, Length=274, Percent_Identity=29.5620437956204, Blast_Score=104, Evalue=2e-23,
Organism=Drosophila melanogaster, GI23397609, Length=274, Percent_Identity=31.3868613138686, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI21357041, Length=273, Percent_Identity=28.5714285714286, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI28571526, Length=273, Percent_Identity=30.03663003663, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24644337, Length=271, Percent_Identity=29.520295202952, Blast_Score=91, Evalue=6e-19,
Organism=Drosophila melanogaster, GI21355319, Length=276, Percent_Identity=27.1739130434783, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24639444, Length=274, Percent_Identity=31.7518248175182, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24644339, Length=276, Percent_Identity=26.0869565217391, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI17737361, Length=212, Percent_Identity=29.2452830188679, Blast_Score=75, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.159 [C]

Molecular weight: Translated: 30514; Mature: 30514

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIPFHVNLKDKVAVVTGGSGILCSGFAKALAQCGAKVAVVSRRAESADRVARDIQENGG
CCCCEEECCCCCEEEEECCCCEEHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHCCC
EALAVTANVLDKQSLEQAKQLINRTFGRCNILINGAGGNHPMGTTDKEYLYKKDLNTKEL
CEEEEEHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHCCCCHHHE
TTFFDLEIEGVRSVLDLNFLGTLLTTQVFAKDMADHDGNVIVNISSMNAYTPLTKIPAYS
EEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHCCCCC
GAKAAVSNFTQWLAVHMSKVGIRVNAIAPGFFLTAQNRSLLLNEDGTYTERAQKIINQTP
CHHHHHHHHHHHHHHHHHHHCEEEEEECCCEEEEECCCEEEEECCCCHHHHHHHHHHCCC
LDRFGVPEDLIGTLLWLVSDKASRFVTGTIIPVDGGFSAYSGV
CHHCCCCHHHHHHHHHHHCCCCCCEEEEEEEEECCCCHHCCCC
>Mature Secondary Structure
MQIPFHVNLKDKVAVVTGGSGILCSGFAKALAQCGAKVAVVSRRAESADRVARDIQENGG
CCCCEEECCCCCEEEEECCCCEEHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHCCC
EALAVTANVLDKQSLEQAKQLINRTFGRCNILINGAGGNHPMGTTDKEYLYKKDLNTKEL
CEEEEEHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHCCCCHHHE
TTFFDLEIEGVRSVLDLNFLGTLLTTQVFAKDMADHDGNVIVNISSMNAYTPLTKIPAYS
EEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHCCCCC
GAKAAVSNFTQWLAVHMSKVGIRVNAIAPGFFLTAQNRSLLLNEDGTYTERAQKIINQTP
CHHHHHHHHHHHHHHHHHHHCEEEEEECCCEEEEECCCEEEEECCCCHHHHHHHHHHCCC
LDRFGVPEDLIGTLLWLVSDKASRFVTGTIIPVDGGFSAYSGV
CHHCCCCHHHHHHHHHHHCCCCCCEEEEEEEEECCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]

Metal ions: CaCl2; KCl; MnCl2; NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.8 {cholic} 0.24 {taurochenodeoxycholic} 0.085 {glycochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]

Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]

Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]

General reaction: Redox reaction [C]

Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579062; 9384377 [H]