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The map label for this gene is brnQ [H]

Identifier: 52785849

GI number: 52785849

Start: 2038263

End: 2039582

Strand: Reverse

Name: brnQ [H]

Synonym: BLi02096

Alternate gene names: 52785849

Gene position: 2039582-2038263 (Counterclockwise)

Preceding gene: 52785850

Following gene: 52785848

Centisome position: 48.3

GC content: 41.21

Gene sequence:

>1320_bases
ATGATGGCAAAAAAAATTCCTTTTTCATTTATCCTAATCATAGGATTAATGCTATTCGCATTATTTTTCGGTGCAGGAAA
TCTCATTTTCCCGCCTATGCTGGGTCAGATGGCCGGAGAGAATGTATGGACAGCCAATGCAGGATTCTTGGTGACCGGAG
TCGGATTGCCATTGCTCGCCATTACGGCATTCGTTTTTTCAGGAAAAAATGATTTGCAGTCTCTGGCCAGTCGTGTGCAT
CCGTTGTTCGGCATTGTATTTACAACGGTTCTCTATTTAGCAATCGGCCCTTTTTTTGCGATTCCCAGATCCGGCAATGT
GTCGTTTGAAATAGGGGTTAAACCTTTTTTATCAAATGATGCGGGCCCTGTTGCCTTAATCATATTTACCGTATTATTTT
TCACTGTGGCATGCTTGCTGTCCCTGAATCCAACTAAAATTATCGATGTAATCGGAAAATTTCTAACTCCTATTAAATTA
ACATTTATCGGATTGCTCGTTGTTACGGCTATTATTCATCCAATCGGAAGCTTTCAAGCTCCGTCTGAAGGATATGCATC
ACACGTATTTTTTAAAGGATTCAAAGAAGGTTACTTTACTCTTGATGCCCTTGTCGCTTTTGTATTTGGAATCATCATTG
TGAACGCCATGAAAGAGAAGGGAGCGGCCTCCAAAAAACAGCTCATAATCGTTTGTGCAAAATCGACGGCGATTGCAGCG
GCGCTCTTGGCTCTGATCTATTCAGCGCTTTCATATATGGGGGCTTCCAGCGTTGAAAAACTGGGTCTTTTAGAAAATGG
AGCCGAGGTTTTGGCAAAGGTTTCATCCTATTATTTCGGACCATATGGTTCCGTTTTTTTGGGCTTAATGATAACTGTAG
CATGTCTGACAACAAGTGTGGGACTCATCACAGCATGCTCTTCGTTTTTCCACGGCTTGTTTCCAAACATCTCCTATAAA
AAAATTGCTGTCATTCTGTCAATGTTCAGCACCCTTGTAGCAAATATAGGATTAACACAGCTGATTAACGTTTCAATGCC
GGTTTTAACAACTTTGTATCCCATTGCGATTTCTTTAATATTCTTAACATTTTTACATCCTTTATTTAAAGGGAAGAGGG
AAGTGTATCAAGGCAGTCTGATATTGACCTTTATCATCAGCCTGTTTGACGGCCTGAATGCTGCCGGACTCAAAATTGCA
GCTATTAATCAATTTTTCAATCAGTTTCTCCCGATGTATGACATCGGATTAGGATGGGTGCTTCCGGCGATTTTAGGAGG
AATAGGCGGCTATCTCATTCATAGACTCCGGAAGTGCTGA

Upstream 100 bases:

>100_bases
ATTTACTGATAATTCAGGACATTTATTTTGAAAATTTTTATCGCGCTACACTGCTGACTACTAAAAACGCACTAATAGCT
TAACCCGAATCGGAGGACTC

Downstream 100 bases:

>100_bases
ATAAACTCATAAATATGAAGGCAACCCTATGATCCCAGGGTTGCCTTTTTCTTTCAAATGTTTGCGATGGTTTTCCATAA
ATGGATATTCTATAATGGTG

Product: BrnQ

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: Branched-chain amino acid uptake carrier BrnQ [H]

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MMAKKIPFSFILIIGLMLFALFFGAGNLIFPPMLGQMAGENVWTANAGFLVTGVGLPLLAITAFVFSGKNDLQSLASRVH
PLFGIVFTTVLYLAIGPFFAIPRSGNVSFEIGVKPFLSNDAGPVALIIFTVLFFTVACLLSLNPTKIIDVIGKFLTPIKL
TFIGLLVVTAIIHPIGSFQAPSEGYASHVFFKGFKEGYFTLDALVAFVFGIIIVNAMKEKGAASKKQLIIVCAKSTAIAA
ALLALIYSALSYMGASSVEKLGLLENGAEVLAKVSSYYFGPYGSVFLGLMITVACLTTSVGLITACSSFFHGLFPNISYK
KIAVILSMFSTLVANIGLTQLINVSMPVLTTLYPIAISLIFLTFLHPLFKGKREVYQGSLILTFIISLFDGLNAAGLKIA
AINQFFNQFLPMYDIGLGWVLPAILGGIGGYLIHRLRKC

Sequences:

>Translated_439_residues
MMAKKIPFSFILIIGLMLFALFFGAGNLIFPPMLGQMAGENVWTANAGFLVTGVGLPLLAITAFVFSGKNDLQSLASRVH
PLFGIVFTTVLYLAIGPFFAIPRSGNVSFEIGVKPFLSNDAGPVALIIFTVLFFTVACLLSLNPTKIIDVIGKFLTPIKL
TFIGLLVVTAIIHPIGSFQAPSEGYASHVFFKGFKEGYFTLDALVAFVFGIIIVNAMKEKGAASKKQLIIVCAKSTAIAA
ALLALIYSALSYMGASSVEKLGLLENGAEVLAKVSSYYFGPYGSVFLGLMITVACLTTSVGLITACSSFFHGLFPNISYK
KIAVILSMFSTLVANIGLTQLINVSMPVLTTLYPIAISLIFLTFLHPLFKGKREVYQGSLILTFIISLFDGLNAAGLKIA
AINQFFNQFLPMYDIGLGWVLPAILGGIGGYLIHRLRKC
>Mature_439_residues
MMAKKIPFSFILIIGLMLFALFFGAGNLIFPPMLGQMAGENVWTANAGFLVTGVGLPLLAITAFVFSGKNDLQSLASRVH
PLFGIVFTTVLYLAIGPFFAIPRSGNVSFEIGVKPFLSNDAGPVALIIFTVLFFTVACLLSLNPTKIIDVIGKFLTPIKL
TFIGLLVVTAIIHPIGSFQAPSEGYASHVFFKGFKEGYFTLDALVAFVFGIIIVNAMKEKGAASKKQLIIVCAKSTAIAA
ALLALIYSALSYMGASSVEKLGLLENGAEVLAKVSSYYFGPYGSVFLGLMITVACLTTSVGLITACSSFFHGLFPNISYK
KIAVILSMFSTLVANIGLTQLINVSMPVLTTLYPIAISLIFLTFLHPLFKGKREVYQGSLILTFIISLFDGLNAAGLKIA
AINQFFNQFLPMYDIGLGWVLPAILGGIGGYLIHRLRKC

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine) Which is coupled to a proton motive force [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=424, Percent_Identity=39.1509433962264, Blast_Score=300, Evalue=9e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 47116; Mature: 47116

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMAKKIPFSFILIIGLMLFALFFGAGNLIFPPMLGQMAGENVWTANAGFLVTGVGLPLLA
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCEEECCCCEEEECCCHHHHH
ITAFVFSGKNDLQSLASRVHPLFGIVFTTVLYLAIGPFFAIPRSGNVSFEIGVKPFLSND
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHCCCC
AGPVALIIFTVLFFTVACLLSLNPTKIIDVIGKFLTPIKLTFIGLLVVTAIIHPIGSFQA
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PSEGYASHVFFKGFKEGYFTLDALVAFVFGIIIVNAMKEKGAASKKQLIIVCAKSTAIAA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHH
ALLALIYSALSYMGASSVEKLGLLENGAEVLAKVSSYYFGPYGSVFLGLMITVACLTTSV
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
GLITACSSFFHGLFPNISYKKIAVILSMFSTLVANIGLTQLINVSMPVLTTLYPIAISLI
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
FLTFLHPLFKGKREVYQGSLILTFIISLFDGLNAAGLKIAAINQFFNQFLPMYDIGLGWV
HHHHHHHHHHCHHHHHHCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHH
LPAILGGIGGYLIHRLRKC
HHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMAKKIPFSFILIIGLMLFALFFGAGNLIFPPMLGQMAGENVWTANAGFLVTGVGLPLLA
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCEEECCCCEEEECCCHHHHH
ITAFVFSGKNDLQSLASRVHPLFGIVFTTVLYLAIGPFFAIPRSGNVSFEIGVKPFLSND
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHCCCC
AGPVALIIFTVLFFTVACLLSLNPTKIIDVIGKFLTPIKLTFIGLLVVTAIIHPIGSFQA
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PSEGYASHVFFKGFKEGYFTLDALVAFVFGIIIVNAMKEKGAASKKQLIIVCAKSTAIAA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHH
ALLALIYSALSYMGASSVEKLGLLENGAEVLAKVSSYYFGPYGSVFLGLMITVACLTTSV
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
GLITACSSFFHGLFPNISYKKIAVILSMFSTLVANIGLTQLINVSMPVLTTLYPIAISLI
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
FLTFLHPLFKGKREVYQGSLILTFIISLFDGLNAAGLKIAAINQFFNQFLPMYDIGLGWV
HHHHHHHHHHCHHHHHHCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHH
LPAILGGIGGYLIHRLRKC
HHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9023181; 9308178; 9384377 [H]