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The map label for this gene is ebrB

Identifier: 52785716

GI number: 52785716

Start: 1916771

End: 1917130

Strand: Reverse

Name: ebrB

Synonym: BLi01958

Alternate gene names: 52785716

Gene position: 1917130-1916771 (Counterclockwise)

Preceding gene: 52785717

Following gene: 52785714

Centisome position: 45.4

GC content: 48.89

Gene sequence:

>360_bases
ATGAAAGGCATGATCTTTTTGGCGGCTGCCATTTTGTCCGAAGTATTCGGAAGCACGATGCTGAAGCTGTCAGAAGGCTT
TAGCGCCCCCCTGCCCGCAGCCGGCGTGATAATCGGTTTTGCGGCTTCTTTTACATTTCTGAGCTTTTCATTAAAAACAC
TGCCTTTATCAGCGGCTTATGCGACTTGGGCCGGCACCGGAACGGCCTTGACAGCTGCAATCGGGCATTTTATATTTCAG
GAGCCATTTAATCTCAAAACTCTTATTGGGCTTACTTTGATCATCGGCGGCGTCTTTCTGCTCAATTCAAAGCGCACCGA
AGCAGCGGATCAAAAGGCTCAGCTCACGATCGAAATCTAA

Upstream 100 bases:

>100_bases
TTGTGGTTTCAAGAAAAGCTGAACCGGCGAAATATCGCCGGCATTATCTGTCTCGTGTCAGGCGTTGTACTGATCAATTT
GTCATAAGGAGGCGAATGTC

Downstream 100 bases:

>100_bases
TCCGCTTTGGAGACGACAATCTTCGGCTCTAATTCAATGGCGACGTTGCCCTGAACCGCCCTTGATATCATGCATGATGT
CTCCGCTTTTTTAGCCAGTC

Product: EbrB

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 119; Mature: 119

Protein sequence:

>119_residues
MKGMIFLAAAILSEVFGSTMLKLSEGFSAPLPAAGVIIGFAASFTFLSFSLKTLPLSAAYATWAGTGTALTAAIGHFIFQ
EPFNLKTLIGLTLIIGGVFLLNSKRTEAADQKAQLTIEI

Sequences:

>Translated_119_residues
MKGMIFLAAAILSEVFGSTMLKLSEGFSAPLPAAGVIIGFAASFTFLSFSLKTLPLSAAYATWAGTGTALTAAIGHFIFQ
EPFNLKTLIGLTLIIGGVFLLNSKRTEAADQKAQLTIEI
>Mature_119_residues
MKGMIFLAAAILSEVFGSTMLKLSEGFSAPLPAAGVIIGFAASFTFLSFSLKTLPLSAAYATWAGTGTALTAAIGHFIFQ
EPFNLKTLIGLTLIIGGVFLLNSKRTEAADQKAQLTIEI

Specific function: Part of a multidrug efflux pump. Confers resistance to cationic lipophilic dyes such as ethidium bromide, acriflavine, pyronine Y and safranin O. The efflux is probably coupled to an influx of protons

COG id: COG2076

COG function: function code P; Membrane transporters of cations and cationic drugs

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the small multidrug resistance (SMR) protein family. EbrA/ebrB (TC 2.A.7.1.6) subfamily

Homologues:

Organism=Escherichia coli, GI1786755, Length=102, Percent_Identity=40.1960784313725, Blast_Score=81, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): EBRB_BACLD (Q65JB2)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_079127.1
- RefSeq:   YP_091545.1
- ProteinModelPortal:   Q65JB2
- EnsemblBacteria:   EBBACT00000055474
- EnsemblBacteria:   EBBACT00000060449
- GeneID:   3031183
- GeneID:   3100548
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi01958
- KEGG:   bli:BL00457
- NMPDR:   fig|279010.5.peg.2661
- eggNOG:   COG2076
- GeneTree:   EBGT00050000001580
- HOGENOM:   HBG740972
- OMA:   MLKLSQG
- ProtClustDB:   CLSK887328
- BioCyc:   BLIC279010-1:BLI01958-MONOMER
- BioCyc:   BLIC279010:BL00457-MONOMER
- GO:   GO:0006810
- InterPro:   IPR000390

Pfam domain/function: PF00893 Multi_Drug_Res

EC number: NA

Molecular weight: Translated: 12473; Mature: 12473

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xe5ade10)-; HASH(0xe99e678)-; HASH(0xfa5be90)-; HASH(0xf899588)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGMIFLAAAILSEVFGSTMLKLSEGFSAPLPAAGVIIGFAASFTFLSFSLKTLPLSAAY
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHH
ATWAGTGTALTAAIGHFIFQEPFNLKTLIGLTLIIGGVFLLNSKRTEAADQKAQLTIEI
HHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHEECCCCCCCCCCCEEEEEEH
>Mature Secondary Structure
MKGMIFLAAAILSEVFGSTMLKLSEGFSAPLPAAGVIIGFAASFTFLSFSLKTLPLSAAY
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHH
ATWAGTGTALTAAIGHFIFQEPFNLKTLIGLTLIIGGVFLLNSKRTEAADQKAQLTIEI
HHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHEECCCCCCCCCCCEEEEEEH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA