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The map label for this gene is ebrA

Identifier: 52785717

GI number: 52785717

Start: 1917144

End: 1917461

Strand: Reverse

Name: ebrA

Synonym: BLi01959

Alternate gene names: 52785717

Gene position: 1917461-1917144 (Counterclockwise)

Preceding gene: 52785726

Following gene: 52785716

Centisome position: 45.41

GC content: 50.94

Gene sequence:

>318_bases
ATGATTGCAGGCTACATTTTTCTTCTGATCGCCATCTTGTCAGAAGCGGCGGCAGCCGCCATGCTGAAAATATCTGACGG
GTTCGCGCGCTGGCAGCCGTCGGTTCTTGTAGTAATCGGCTACGGTCTCGCATTTTACATGATGTCGCTCACATTGCAGG
TCATTCCATTAAGCCTCTCCTATGCGACTTGGAGCGGAGCGGGCACCGTTCTGACTGCAATCATCGGCGTCTTGTGGTTT
CAAGAAAAGCTGAACCGGCGAAATATCGCCGGCATTATCTGTCTCGTGTCAGGCGTTGTACTGATCAATTTGTCATAA

Upstream 100 bases:

>100_bases
GCATCTGGCTTTCGGGAATGTTTCAATTGGAACTGCTGGCTGACGAAGAGAAAGCTCTCCTTTTGAATCATGCAATAAAA
TCGATAGAGGAGGATTCCGT

Downstream 100 bases:

>100_bases
GGAGGCGAATGTCATGAAAGGCATGATCTTTTTGGCGGCTGCCATTTTGTCCGAAGTATTCGGAAGCACGATGCTGAAGC
TGTCAGAAGGCTTTAGCGCC

Product: EbrA

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 105; Mature: 105

Protein sequence:

>105_residues
MIAGYIFLLIAILSEAAAAAMLKISDGFARWQPSVLVVIGYGLAFYMMSLTLQVIPLSLSYATWSGAGTVLTAIIGVLWF
QEKLNRRNIAGIICLVSGVVLINLS

Sequences:

>Translated_105_residues
MIAGYIFLLIAILSEAAAAAMLKISDGFARWQPSVLVVIGYGLAFYMMSLTLQVIPLSLSYATWSGAGTVLTAIIGVLWF
QEKLNRRNIAGIICLVSGVVLINLS
>Mature_105_residues
MIAGYIFLLIAILSEAAAAAMLKISDGFARWQPSVLVVIGYGLAFYMMSLTLQVIPLSLSYATWSGAGTVLTAIIGVLWF
QEKLNRRNIAGIICLVSGVVLINLS

Specific function: Part of a multidrug efflux pump. Confers resistance to cationic lipophilic dyes such as ethidium bromide, acriflavine, pyronine Y and safranin O. The efflux is probably coupled to an influx of protons

COG id: COG2076

COG function: function code P; Membrane transporters of cations and cationic drugs

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the small multidrug resistance (SMR) protein family. EbrA/ebrB (TC 2.A.7.1.6) subfamily

Homologues:

Organism=Escherichia coli, GI1786755, Length=100, Percent_Identity=39, Blast_Score=73, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): EBRA_BACLD (Q65JB1)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_079128.1
- RefSeq:   YP_091546.1
- ProteinModelPortal:   Q65JB1
- STRING:   Q65JB1
- EnsemblBacteria:   EBBACT00000054195
- EnsemblBacteria:   EBBACT00000060374
- GeneID:   3031184
- GeneID:   3100690
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi01959
- KEGG:   bli:BL05171
- NMPDR:   fig|279010.5.peg.2662
- eggNOG:   COG2076
- GeneTree:   EBGT00050000001580
- HOGENOM:   HBG740972
- OMA:   RILFREP
- ProtClustDB:   CLSK887329
- BioCyc:   BLIC279010-1:BLI01959-MONOMER
- BioCyc:   BLIC279010:BL05171-MONOMER
- GO:   GO:0006810
- InterPro:   IPR000390

Pfam domain/function: PF00893 Multi_Drug_Res

EC number: NA

Molecular weight: Translated: 11265; Mature: 11265

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xea2f1c4)-; HASH(0xe312d18)-; HASH(0xe9531f4)-; HASH(0xf014ef0)-;

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAGYIFLLIAILSEAAAAAMLKISDGFARWQPSVLVVIGYGLAFYMMSLTLQVIPLSLS
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHEEEEE
YATWSGAGTVLTAIIGVLWFQEKLNRRNIAGIICLVSGVVLINLS
ECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCEEEEECH
>Mature Secondary Structure
MIAGYIFLLIAILSEAAAAAMLKISDGFARWQPSVLVVIGYGLAFYMMSLTLQVIPLSLS
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHEEEEE
YATWSGAGTVLTAIIGVLWFQEKLNRRNIAGIICLVSGVVLINLS
ECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCEEEEECH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA