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The map label for this gene is ligD [H]

Identifier: 52785259

GI number: 52785259

Start: 1465910

End: 1467760

Strand: Reverse

Name: ligD [H]

Synonym: BLi01494

Alternate gene names: 52785259

Gene position: 1467760-1465910 (Counterclockwise)

Preceding gene: 52785260

Following gene: 52785257

Centisome position: 34.76

GC content: 46.84

Gene sequence:

>1851_bases
ATGGGAATTACGATGCAGCCTGTATTGACTACATCAGCTCCCTCAGGCGGCAATTGGCGGTATGAAGTCAAATATGACGG
GTATCGGGGTCTTTTAAAGATTGCTGCGACCGGCGAAGTTTCGCTCATCAGCAGAAACTCACAGCCGCTCGAAAACACAT
TTCCGGAAATTTCGGAATTTGCAAAAAGCATGATCGAAACTCTCAAAGAACACTTGCCGATCACGATTGACGGGGAAATT
GTGTCGTTGACAAACCGTTTTCGTTCCCGATTTGAATATGTCCAAAAAAGGGGTTTGAGCAAAAAGGCCGAGTTGATTGC
ACAAGCAGCCGCAAAGAAACCGTGCCAGTACTTGGCTTTTGATTTACTGGTTTTTAACGGAGAAAGTTTAACCTCGCTCC
CCTATACCGAACGAAAACGCATGCTTTTCGACCTGATGAAAGAGCTCGGCCTGCCGATGGCTCCCGATCCGATGGCTCAC
GCTCGAATTCAATACATCCCTGACACTTCTGATTTCCACGACCTTTGGAAGGCTGTTAAAAGGTTTGACGGCGAGGGAAT
TGTCGCGAAGAAAAAAGACAGCCGCTGGGCTGAAAATAAAAAAACGGCCGAATGGCTCAAACTCAAAAACTACAAAAAAG
CTGCTGTATTTATGACAGGCTACAACATGGCAAACCGTTATTTGACAATCGCCGTCTATGACCGCGGCCAAATCAAGGAA
GTCGGTTCGGTTTCCCACGGACTCGGAGAACAGGAAAGAAACGCCATTCTTTCGATCGTCAAGCAATACGGAACCGAAAC
AAAACCCGGTGAATATACCATTGATCCGTCGATTTGCATGACGGTCCATTACTTAACGATTCATTACGGGACATTGCGCG
AGGTGTCGTTTGTTTCATTTGAATTTGACATGGCATGGGAAAATTGCACTTACAACCGCCTGCTGCTTCATTCAAGAAAC
GTCCATCCCGATTTGCAGCTGACAAGCCTTGATAAAGTCATCTTTCCTAAAAGCGATAAAACAAAAGCGGATTACATCGG
ATACCTTAATGAAATCGGCGATTTTCTGCTTCCGTTTCTCGACAATCGGGCGCTTACCGTTATCCGTTATCCGCACGGTT
CCGGCGGAGAGTCTTTTTTTCAAAAAAACAAGCCCGACTACGCACCCGAATTTATCACGTCCATTCGGGATGACGAGCAC
GAGCATATCATCTGCAGCGATTATTCGGTTCTGCTGTGGCTTGCCAATCAGCTTGCGTTGGAGTTCCATATCCCTTTTCA
AACGGTGGATACGGCCCATCCGACCGAAATCGTTTTCGATCTTGATCCGCCTTCAAAGTCGGAGTTTCCGCTGGCCGTCA
GAGCCGCGAAAGAGCTGCACCGTTTATTTGATCAGCTCGGCCTCTTATCTTTTCCAAAGCTGTCGGGAAATAAAGGGATA
CAAATCTATATTCCGATTTCAAAAAACGCCTTTACTTATGAAGAAACCCGCCTTTTCACAAGCTTTGCGGCTTCTTATTG
CGTCTCGCTTTTTCCCGATCTGTTCACAACCGAACGCCTGATCAAAAACAGGGGCGGAAAGCTCTATATTGACTATGTGC
AGCATGCCCCGGGAAAAACGATCATCTGCCCGTATTCCACAAGAGGCAATGAGCTTGGCACAGTGGCCGCACCGCTGTTT
TGGGATGAAGTCCATTCAGACTTGTCACCCTTAAATTTTACGATGGAGGCTGTCGTCAAACGCACAAAGGAACTCGGATG
CCCTTTCGAAAGCTTCTTCAGACAGCCCCAGGACAAACAGATCAAAGCTATTCTCGATCACTTAAAAGAAAACAACCGTT
CGGAAAACTGA

Upstream 100 bases:

>100_bases
AGGGCCTGACCGGGAAGCGGCGAAAGCGCCTGCAGCAAAAGGAACAGCTGAGAAAAAGAAAAAAACGTCGCGCAAAAAAG
CTTCAGAGGCAAAATAAAAA

Downstream 100 bases:

>100_bases
CTTTTTCAGCGAAACAATAGGTGCTGGCTGTGGATTCCGGCTTTTTTCAGAAGGACCATCAGCTGCTCCTGTTCCTCCTT
TGTCAAGCCTGAAAAAGCCC

Product: ATP-dependent DNA ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 616; Mature: 615

Protein sequence:

>616_residues
MGITMQPVLTTSAPSGGNWRYEVKYDGYRGLLKIAATGEVSLISRNSQPLENTFPEISEFAKSMIETLKEHLPITIDGEI
VSLTNRFRSRFEYVQKRGLSKKAELIAQAAAKKPCQYLAFDLLVFNGESLTSLPYTERKRMLFDLMKELGLPMAPDPMAH
ARIQYIPDTSDFHDLWKAVKRFDGEGIVAKKKDSRWAENKKTAEWLKLKNYKKAAVFMTGYNMANRYLTIAVYDRGQIKE
VGSVSHGLGEQERNAILSIVKQYGTETKPGEYTIDPSICMTVHYLTIHYGTLREVSFVSFEFDMAWENCTYNRLLLHSRN
VHPDLQLTSLDKVIFPKSDKTKADYIGYLNEIGDFLLPFLDNRALTVIRYPHGSGGESFFQKNKPDYAPEFITSIRDDEH
EHIICSDYSVLLWLANQLALEFHIPFQTVDTAHPTEIVFDLDPPSKSEFPLAVRAAKELHRLFDQLGLLSFPKLSGNKGI
QIYIPISKNAFTYEETRLFTSFAASYCVSLFPDLFTTERLIKNRGGKLYIDYVQHAPGKTIICPYSTRGNELGTVAAPLF
WDEVHSDLSPLNFTMEAVVKRTKELGCPFESFFRQPQDKQIKAILDHLKENNRSEN

Sequences:

>Translated_616_residues
MGITMQPVLTTSAPSGGNWRYEVKYDGYRGLLKIAATGEVSLISRNSQPLENTFPEISEFAKSMIETLKEHLPITIDGEI
VSLTNRFRSRFEYVQKRGLSKKAELIAQAAAKKPCQYLAFDLLVFNGESLTSLPYTERKRMLFDLMKELGLPMAPDPMAH
ARIQYIPDTSDFHDLWKAVKRFDGEGIVAKKKDSRWAENKKTAEWLKLKNYKKAAVFMTGYNMANRYLTIAVYDRGQIKE
VGSVSHGLGEQERNAILSIVKQYGTETKPGEYTIDPSICMTVHYLTIHYGTLREVSFVSFEFDMAWENCTYNRLLLHSRN
VHPDLQLTSLDKVIFPKSDKTKADYIGYLNEIGDFLLPFLDNRALTVIRYPHGSGGESFFQKNKPDYAPEFITSIRDDEH
EHIICSDYSVLLWLANQLALEFHIPFQTVDTAHPTEIVFDLDPPSKSEFPLAVRAAKELHRLFDQLGLLSFPKLSGNKGI
QIYIPISKNAFTYEETRLFTSFAASYCVSLFPDLFTTERLIKNRGGKLYIDYVQHAPGKTIICPYSTRGNELGTVAAPLF
WDEVHSDLSPLNFTMEAVVKRTKELGCPFESFFRQPQDKQIKAILDHLKENNRSEN
>Mature_615_residues
GITMQPVLTTSAPSGGNWRYEVKYDGYRGLLKIAATGEVSLISRNSQPLENTFPEISEFAKSMIETLKEHLPITIDGEIV
SLTNRFRSRFEYVQKRGLSKKAELIAQAAAKKPCQYLAFDLLVFNGESLTSLPYTERKRMLFDLMKELGLPMAPDPMAHA
RIQYIPDTSDFHDLWKAVKRFDGEGIVAKKKDSRWAENKKTAEWLKLKNYKKAAVFMTGYNMANRYLTIAVYDRGQIKEV
GSVSHGLGEQERNAILSIVKQYGTETKPGEYTIDPSICMTVHYLTIHYGTLREVSFVSFEFDMAWENCTYNRLLLHSRNV
HPDLQLTSLDKVIFPKSDKTKADYIGYLNEIGDFLLPFLDNRALTVIRYPHGSGGESFFQKNKPDYAPEFITSIRDDEHE
HIICSDYSVLLWLANQLALEFHIPFQTVDTAHPTEIVFDLDPPSKSEFPLAVRAAKELHRLFDQLGLLSFPKLSGNKGIQ
IYIPISKNAFTYEETRLFTSFAASYCVSLFPDLFTTERLIKNRGGKLYIDYVQHAPGKTIICPYSTRGNELGTVAAPLFW
DEVHSDLSPLNFTMEAVVKRTKELGCPFESFFRQPQDKQIKAILDHLKENNRSEN

Specific function: Probably involved in the repair of DNA double-strand breaks by non-homologous-end joining (NHEJ) during spore germination [H]

COG id: COG3285

COG function: function code L; Predicted eukaryotic-type DNA primase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent DNA ligase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012310
- InterPro:   IPR014146
- InterPro:   IPR014145
- InterPro:   IPR014143 [H]

Pfam domain/function: PF01068 DNA_ligase_A_M [H]

EC number: =6.5.1.1 [H]

Molecular weight: Translated: 70286; Mature: 70155

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: PS50160 DNA_LIGASE_A3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGITMQPVLTTSAPSGGNWRYEVKYDGYRGLLKIAATGEVSLISRNSQPLENTFPEISEF
CCCCCCCEEEECCCCCCCEEEEEEECCCCEEEEEEECCCEEEEECCCCCHHHCCHHHHHH
AKSMIETLKEHLPITIDGEIVSLTNRFRSRFEYVQKRGLSKKAELIAQAAAKKPCQYLAF
HHHHHHHHHHHCCEEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHEE
DLLVFNGESLTSLPYTERKRMLFDLMKELGLPMAPDPMAHARIQYIPDTSDFHDLWKAVK
EEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHH
RFDGEGIVAKKKDSRWAENKKTAEWLKLKNYKKAAVFMTGYNMANRYLTIAVYDRGQIKE
HCCCCCEEEECCCCCHHCCCHHHHHHHHCCCCEEEEEEECCCCCCEEEEEEEECCCCCHH
VGSVSHGLGEQERNAILSIVKQYGTETKPGEYTIDPSICMTVHYLTIHYGTLREVSFVSF
HHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCEEECCHHHEEEEEEEEECCCHHCEEEEEE
EFDMAWENCTYNRLLLHSRNVHPDLQLTSLDKVIFPKSDKTKADYIGYLNEIGDFLLPFL
EEEECCCCCCCCEEEEECCCCCCCCEEECCCCEECCCCCCCHHHHHHHHHHHHHHHHHHH
DNRALTVIRYPHGSGGESFFQKNKPDYAPEFITSIRDDEHEHIICSDYSVLLWLANQLAL
CCCEEEEEECCCCCCCHHHHHCCCCCCCHHHHHHHCCCCCCEEEECCHHHHHHHHHHHEE
EFHIPFQTVDTAHPTEIVFDLDPPSKSEFPLAVRAAKELHRLFDQLGLLSFPKLSGNKGI
EEECCCEECCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCE
QIYIPISKNAFTYEETRLFTSFAASYCVSLFPDLFTTERLIKNRGGKLYIDYVQHAPGKT
EEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCE
IICPYSTRGNELGTVAAPLFWDEVHSDLSPLNFTMEAVVKRTKELGCPFESFFRQPQDKQ
EEEECCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCCCCHH
IKAILDHLKENNRSEN
HHHHHHHHHHCCCCCC
>Mature Secondary Structure 
GITMQPVLTTSAPSGGNWRYEVKYDGYRGLLKIAATGEVSLISRNSQPLENTFPEISEF
CCCCCCEEEECCCCCCCEEEEEEECCCCEEEEEEECCCEEEEECCCCCHHHCCHHHHHH
AKSMIETLKEHLPITIDGEIVSLTNRFRSRFEYVQKRGLSKKAELIAQAAAKKPCQYLAF
HHHHHHHHHHHCCEEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHEE
DLLVFNGESLTSLPYTERKRMLFDLMKELGLPMAPDPMAHARIQYIPDTSDFHDLWKAVK
EEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHH
RFDGEGIVAKKKDSRWAENKKTAEWLKLKNYKKAAVFMTGYNMANRYLTIAVYDRGQIKE
HCCCCCEEEECCCCCHHCCCHHHHHHHHCCCCEEEEEEECCCCCCEEEEEEEECCCCCHH
VGSVSHGLGEQERNAILSIVKQYGTETKPGEYTIDPSICMTVHYLTIHYGTLREVSFVSF
HHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCEEECCHHHEEEEEEEEECCCHHCEEEEEE
EFDMAWENCTYNRLLLHSRNVHPDLQLTSLDKVIFPKSDKTKADYIGYLNEIGDFLLPFL
EEEECCCCCCCCEEEEECCCCCCCCEEECCCCEECCCCCCCHHHHHHHHHHHHHHHHHHH
DNRALTVIRYPHGSGGESFFQKNKPDYAPEFITSIRDDEHEHIICSDYSVLLWLANQLAL
CCCEEEEEECCCCCCCHHHHHCCCCCCCHHHHHHHCCCCCCEEEECCHHHHHHHHHHHEE
EFHIPFQTVDTAHPTEIVFDLDPPSKSEFPLAVRAAKELHRLFDQLGLLSFPKLSGNKGI
EEECCCEECCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCE
QIYIPISKNAFTYEETRLFTSFAASYCVSLFPDLFTTERLIKNRGGKLYIDYVQHAPGKT
EEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCE
IICPYSTRGNELGTVAAPLFWDEVHSDLSPLNFTMEAVVKRTKELGCPFESFFRQPQDKQ
EEEECCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCCCCHH
IKAILDHLKENNRSEN
HHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]