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The map label for this gene is lmrB [H]

Identifier: 52785078

GI number: 52785078

Start: 1311155

End: 1312579

Strand: Reverse

Name: lmrB [H]

Synonym: BLi01310

Alternate gene names: 52785078

Gene position: 1312579-1311155 (Counterclockwise)

Preceding gene: 52785079

Following gene: 52785076

Centisome position: 31.08

GC content: 50.11

Gene sequence:

>1425_bases
ATGGAACAAGCTAGAAAAGGCTCCTTCATGATTATCGCAATCTTAATCGTCGGAAATTTTCTCGCTCTGATTAATGAGAC
GGTCATGAATGTGGCTTTGCCGAAAATCATCGACGATTTTGGCATTTCAGCGAACACAGCCCAGTGGCTGTCAACCGGAT
ATATGCTCATGATCGGCATCGCCGTGCCGATTACCGCGTTTTTAATGCAGCGCTTTACGACAAGGCAGCTGTTTCTGTCT
GCGATGACGCTTTTTACAGCGGGAACCTTGCTGGCCGGAATTGCTCCGGTCTTCCCGCTCGTTCTCGCCGGACGGATCAT
CCAGGGTTCAGGTACAGGCCTGATATTGCCGCTTGTGATGAATGTGATACTGACTTTGATTCCGCCGCAAAAACGCGGAA
CGATGACCGGGATCCTTACGCTCGTTATCTTATTTGCCCCGGCGATCGGTCCGGTCATCTCAGGCTATATTGTCGAACAT
TATTCATGGCGCGTTGTTTTCTTCATGATGCTGCCAGTATCGGTCTTCATGATTTTTTATGCGGCGTTCCGCTTGAAAAA
TGTAACCGAACTGACGTATCCGAAAATTGACATATTATCAATTCTTTTATCGACCGTCGGATTTGGCGGGATCGTTTTCG
GCTTCAGCAACGCCGGGGAAGCAGCGGGCTGGAGCAGTCCGAAAGTCATCATCGGACTGGCGGCCGGTGTCATCGGATTA
ATATTGTTCGTCATGCGCCAGCTCAGATTGGAAGTGCCGATGCTTGATATGAGGCCGTTTCGTTCAAAGATATTTTCAAT
CTCGGTTATCCTGATGTGGATCGTGATGATGCTGCAGTTTTCGATGATGCTGATTTTGCCGCTTTACTTCCAAATGGCGC
TTGAGCTGTCACCGCTTTATACAGGCATTCTCATGCTGCCGGGCGGCCTCGTCCTCGCGATGACATCCCTTGTTGCAGGG
CGCCTGTTTGATAAGCTTGGCTTTAAACCGCTGTTGATCACCGGATTGATCATTGTGATTGCCGTCCTTTCGCTGTTTAC
GAATATTTCAAGCGATACGCCGATGGCGGTCGGAATGATGCTCTACGCCGGCTTTACACTCGGCGTCGGGTTGACAATGG
CTCCGATTATGACGCTCGGTTTGAATCAGGTGCCCAAACCGCTTTACCCGCATGGTTCGGCGATAATGAATACAGTTAAT
CAAGTTTCAGGGGCAATCGGTCCGGCATTGTATACGACGATCCTGACAACCGTTTCGAATCGCTTTATCGCGCACTCTTC
CGGAGGCAGCCGTCAGGAGCTGCACATGCAAGGCATGACAGAAGGCGTTCATATGGTTTATTTCGTAGCCATCGCCTTTG
CAGCCGCCGCTCTTGCGATTTCGGTTTTTGTAAAAAGCAGACGCGCGGAACAGCATGCAGCATAA

Upstream 100 bases:

>100_bases
GTATGTGCTCTCAGTGACGAAAAAAAACGGCCGGCCTCTCAGTGCAGCCGCCGAACAAATTCCTTTTTTGCTAAAACGTT
CATAAAGGGTGGGAATACAA

Downstream 100 bases:

>100_bases
ACATCGTTCGACAAAAAAATGCAGGAGGCAATTAACGCCTCCTGCATTTTTTATATCAGTTTAAAATTTTAACCTTTACG
GTTTTGACGCCCCAATTGAA

Product: hypothetical protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MEQARKGSFMIIAILIVGNFLALINETVMNVALPKIIDDFGISANTAQWLSTGYMLMIGIAVPITAFLMQRFTTRQLFLS
AMTLFTAGTLLAGIAPVFPLVLAGRIIQGSGTGLILPLVMNVILTLIPPQKRGTMTGILTLVILFAPAIGPVISGYIVEH
YSWRVVFFMMLPVSVFMIFYAAFRLKNVTELTYPKIDILSILLSTVGFGGIVFGFSNAGEAAGWSSPKVIIGLAAGVIGL
ILFVMRQLRLEVPMLDMRPFRSKIFSISVILMWIVMMLQFSMMLILPLYFQMALELSPLYTGILMLPGGLVLAMTSLVAG
RLFDKLGFKPLLITGLIIVIAVLSLFTNISSDTPMAVGMMLYAGFTLGVGLTMAPIMTLGLNQVPKPLYPHGSAIMNTVN
QVSGAIGPALYTTILTTVSNRFIAHSSGGSRQELHMQGMTEGVHMVYFVAIAFAAAALAISVFVKSRRAEQHAA

Sequences:

>Translated_474_residues
MEQARKGSFMIIAILIVGNFLALINETVMNVALPKIIDDFGISANTAQWLSTGYMLMIGIAVPITAFLMQRFTTRQLFLS
AMTLFTAGTLLAGIAPVFPLVLAGRIIQGSGTGLILPLVMNVILTLIPPQKRGTMTGILTLVILFAPAIGPVISGYIVEH
YSWRVVFFMMLPVSVFMIFYAAFRLKNVTELTYPKIDILSILLSTVGFGGIVFGFSNAGEAAGWSSPKVIIGLAAGVIGL
ILFVMRQLRLEVPMLDMRPFRSKIFSISVILMWIVMMLQFSMMLILPLYFQMALELSPLYTGILMLPGGLVLAMTSLVAG
RLFDKLGFKPLLITGLIIVIAVLSLFTNISSDTPMAVGMMLYAGFTLGVGLTMAPIMTLGLNQVPKPLYPHGSAIMNTVN
QVSGAIGPALYTTILTTVSNRFIAHSSGGSRQELHMQGMTEGVHMVYFVAIAFAAAALAISVFVKSRRAEQHAA
>Mature_474_residues
MEQARKGSFMIIAILIVGNFLALINETVMNVALPKIIDDFGISANTAQWLSTGYMLMIGIAVPITAFLMQRFTTRQLFLS
AMTLFTAGTLLAGIAPVFPLVLAGRIIQGSGTGLILPLVMNVILTLIPPQKRGTMTGILTLVILFAPAIGPVISGYIVEH
YSWRVVFFMMLPVSVFMIFYAAFRLKNVTELTYPKIDILSILLSTVGFGGIVFGFSNAGEAAGWSSPKVIIGLAAGVIGL
ILFVMRQLRLEVPMLDMRPFRSKIFSISVILMWIVMMLQFSMMLILPLYFQMALELSPLYTGILMLPGGLVLAMTSLVAG
RLFDKLGFKPLLITGLIIVIAVLSLFTNISSDTPMAVGMMLYAGFTLGVGLTMAPIMTLGLNQVPKPLYPHGSAIMNTVN
QVSGAIGPALYTTILTTVSNRFIAHSSGGSRQELHMQGMTEGVHMVYFVAIAFAAAALAISVFVKSRRAEQHAA

Specific function: Proton-dependent transporter. May mediate the efflux of lincomycin [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=451, Percent_Identity=24.8337028824834, Blast_Score=154, Evalue=9e-39,
Organism=Escherichia coli, GI1788710, Length=444, Percent_Identity=24.5495495495495, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI1790195, Length=406, Percent_Identity=24.384236453202, Blast_Score=112, Evalue=7e-26,
Organism=Escherichia coli, GI87081983, Length=422, Percent_Identity=26.303317535545, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1788392, Length=424, Percent_Identity=22.1698113207547, Blast_Score=79, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6325455, Length=376, Percent_Identity=22.8723404255319, Blast_Score=85, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6322958, Length=431, Percent_Identity=22.969837587007, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6319783, Length=336, Percent_Identity=23.2142857142857, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 51232; Mature: 51232

Theoretical pI: Translated: 10.35; Mature: 10.35

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
6.8 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
6.8 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQARKGSFMIIAILIVGNFLALINETVMNVALPKIIDDFGISANTAQWLSTGYMLMIGI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHH
AVPITAFLMQRFTTRQLFLSAMTLFTAGTLLAGIAPVFPLVLAGRIIQGSGTGLILPLVM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHEECCCCCCHHHHHH
NVILTLIPPQKRGTMTGILTLVILFAPAIGPVISGYIVEHYSWRVVFFMMLPVSVFMIFY
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAFRLKNVTELTYPKIDILSILLSTVGFGGIVFGFSNAGEAAGWSSPKVIIGLAAGVIGL
HHHHHCCCHHHCCCHHHHHHHHHHHHCCCHHEEEECCCCCCCCCCCCHHHHHHHHHHHHH
ILFVMRQLRLEVPMLDMRPFRSKIFSISVILMWIVMMLQFSMMLILPLYFQMALELSPLY
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGILMLPGGLVLAMTSLVAGRLFDKLGFKPLLITGLIIVIAVLSLFTNISSDTPMAVGMM
HHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
LYAGFTLGVGLTMAPIMTLGLNQVPKPLYPHGSAIMNTVNQVSGAIGPALYTTILTTVSN
HHHHHHHHHHHHHHHHHHHCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RFIAHSSGGSRQELHMQGMTEGVHMVYFVAIAFAAAALAISVFVKSRRAEQHAA
HHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEQARKGSFMIIAILIVGNFLALINETVMNVALPKIIDDFGISANTAQWLSTGYMLMIGI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHH
AVPITAFLMQRFTTRQLFLSAMTLFTAGTLLAGIAPVFPLVLAGRIIQGSGTGLILPLVM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHEECCCCCCHHHHHH
NVILTLIPPQKRGTMTGILTLVILFAPAIGPVISGYIVEHYSWRVVFFMMLPVSVFMIFY
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAFRLKNVTELTYPKIDILSILLSTVGFGGIVFGFSNAGEAAGWSSPKVIIGLAAGVIGL
HHHHHCCCHHHCCCHHHHHHHHHHHHCCCHHEEEECCCCCCCCCCCCHHHHHHHHHHHHH
ILFVMRQLRLEVPMLDMRPFRSKIFSISVILMWIVMMLQFSMMLILPLYFQMALELSPLY
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGILMLPGGLVLAMTSLVAGRLFDKLGFKPLLITGLIIVIAVLSLFTNISSDTPMAVGMM
HHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
LYAGFTLGVGLTMAPIMTLGLNQVPKPLYPHGSAIMNTVNQVSGAIGPALYTTILTTVSN
HHHHHHHHHHHHHHHHHHHCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RFIAHSSGGSRQELHMQGMTEGVHMVYFVAIAFAAAALAISVFVKSRRAEQHAA
HHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274031; 9384377 [H]