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The map label for this gene is citA [H]

Identifier: 52784782

GI number: 52784782

Start: 1012599

End: 1013690

Strand: Direct

Name: citA [H]

Synonym: BLi01010

Alternate gene names: 52784782

Gene position: 1012599-1013690 (Clockwise)

Preceding gene: 52784780

Following gene: 52784783

Centisome position: 23.98

GC content: 50.18

Gene sequence:

>1092_bases
ATGGTTTATCATGGGTTAAAAGGGATTACTTGTGTTGAAACAGCCATCAGCCGCATTGACGGTGAAAAAGGAAAGCTGAT
CTACAGAGGGTACAATGCTGATGAACTGGCGCTTTCGTCCACATTTGAAGAAGTCGCTTACCTGATTCTCAACGGGGCAT
TTCCGACGGAAGCTGAACTCAGCAGATTCAATGAGCTGCTCGCTTCATTTCGTGTGCTTCCAGAAGCATGCGAGCGATTG
ATCCGCAGTCTTCCGGCCGAATTAGACGATATGGCGGTTCTGCGCACGGCCGTCTCTTCATTCGGGGACATATCGTTTGC
GTTCAAGCCGACAATGGAGCAGGCCCTCAGGCTGATTGCGGCCGTCCCGTCAATTATCGCGTTCAGAAAGAGGCTGGCTG
ATGGAGAAGAGCCTGTTCAGCCGCTGGATGAGCTAGGTTTTGTGGAAAATTATTTTTACATGCTGAAAGGAAAGCGTCCG
ACAGAAGCTCAGAAAAAAGCGCTTGAAACGTACATGATTCTTGCGATGGAACATGGCATGAACGCTTCAACCTTTTCGGC
AAGGGTTACGGTGTCTACGGAATCCGATCTTGTTTCTGCCGTCACATCTGCACTTGGCACGATGAAAGGACCTTTGCACG
GCGGAGCGCCGTCTGCGGTGACGGATATGCTGGAAGGCATCGGGGAAAAACAGCACGCTGAAAGCTATATAAGAGAAAAG
CTTGAAAAAGGCGAGAGACTGATGGGATTTGGACACCGCGTCTATAAAACGCATGATCCGAGAGCGAAGGCTTTAAGGGT
GAAAGCCGAGGAAATTGCCGGGGGCGACAGGGATATCGACCTTGCTCTTCATGTTGAAAATACGGCGATCCGCCTTTTGG
AAGAATACAAGCCGGGCCGCAAGCTTTATACGAATGTCGAATTTTACGCAGCCGCTGTGATGAAGGCGATCGATTTTGAT
GCCGCATTGTTCACCCCTACTTTTTCGGCAGCGAGAATGGTCGGCTGGTGCGCCCATGTGCTTGAGCAGGCTGACAACAA
CATGATTTTCAGGCCATCGGCTAAATATGTCGGAGAATTGGTCTCACAGTAA

Upstream 100 bases:

>100_bases
CTGATTAATTTTATTAATCAAAGACATCAATATAATTCAATATTTTTTAAATATTATATCAACTATAATAATATTTACAA
AGGAAAAAGGAGGAATTGAT

Downstream 100 bases:

>100_bases
TGCGGACACACGCCTTCTCATCGAAGCGCGTGTTTTTTAATAGGATTTTTTGAATGTTTAGTAGGGAATCATGCCGGTGC
TCTTGAATGTATTCATCAAG

Product: hypothetical protein

Products: NA

Alternate protein names: Citrate synthase I [H]

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MVYHGLKGITCVETAISRIDGEKGKLIYRGYNADELALSSTFEEVAYLILNGAFPTEAELSRFNELLASFRVLPEACERL
IRSLPAELDDMAVLRTAVSSFGDISFAFKPTMEQALRLIAAVPSIIAFRKRLADGEEPVQPLDELGFVENYFYMLKGKRP
TEAQKKALETYMILAMEHGMNASTFSARVTVSTESDLVSAVTSALGTMKGPLHGGAPSAVTDMLEGIGEKQHAESYIREK
LEKGERLMGFGHRVYKTHDPRAKALRVKAEEIAGGDRDIDLALHVENTAIRLLEEYKPGRKLYTNVEFYAAAVMKAIDFD
AALFTPTFSAARMVGWCAHVLEQADNNMIFRPSAKYVGELVSQ

Sequences:

>Translated_363_residues
MVYHGLKGITCVETAISRIDGEKGKLIYRGYNADELALSSTFEEVAYLILNGAFPTEAELSRFNELLASFRVLPEACERL
IRSLPAELDDMAVLRTAVSSFGDISFAFKPTMEQALRLIAAVPSIIAFRKRLADGEEPVQPLDELGFVENYFYMLKGKRP
TEAQKKALETYMILAMEHGMNASTFSARVTVSTESDLVSAVTSALGTMKGPLHGGAPSAVTDMLEGIGEKQHAESYIREK
LEKGERLMGFGHRVYKTHDPRAKALRVKAEEIAGGDRDIDLALHVENTAIRLLEEYKPGRKLYTNVEFYAAAVMKAIDFD
AALFTPTFSAARMVGWCAHVLEQADNNMIFRPSAKYVGELVSQ
>Mature_363_residues
MVYHGLKGITCVETAISRIDGEKGKLIYRGYNADELALSSTFEEVAYLILNGAFPTEAELSRFNELLASFRVLPEACERL
IRSLPAELDDMAVLRTAVSSFGDISFAFKPTMEQALRLIAAVPSIIAFRKRLADGEEPVQPLDELGFVENYFYMLKGKRP
TEAQKKALETYMILAMEHGMNASTFSARVTVSTESDLVSAVTSALGTMKGPLHGGAPSAVTDMLEGIGEKQHAESYIREK
LEKGERLMGFGHRVYKTHDPRAKALRVKAEEIAGGDRDIDLALHVENTAIRLLEEYKPGRKLYTNVEFYAAAVMKAIDFD
AALFTPTFSAARMVGWCAHVLEQADNNMIFRPSAKYVGELVSQ

Specific function: Might regulate the synthesis and function of enzymes involved in later enzymatic steps of Krebs cycle. Loss in activity results in sporulation defect [H]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=375, Percent_Identity=23.7333333333333, Blast_Score=76, Evalue=4e-14,
Organism=Escherichia coli, GI1786527, Length=361, Percent_Identity=33.2409972299169, Blast_Score=204, Evalue=8e-54,
Organism=Escherichia coli, GI1786939, Length=372, Percent_Identity=31.989247311828, Blast_Score=196, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI17555174, Length=369, Percent_Identity=23.5772357723577, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6319850, Length=388, Percent_Identity=24.4845360824742, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6324328, Length=395, Percent_Identity=24.5569620253165, Blast_Score=80, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6325257, Length=341, Percent_Identity=22.8739002932551, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24640126, Length=390, Percent_Identity=22.3076923076923, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24640124, Length=390, Percent_Identity=22.3076923076923, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21356863, Length=369, Percent_Identity=24.1192411924119, Blast_Score=75, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 40134; Mature: 40134

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVYHGLKGITCVETAISRIDGEKGKLIYRGYNADELALSSTFEEVAYLILNGAFPTEAEL
CCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCHHHH
SRFNELLASFRVLPEACERLIRSLPAELDDMAVLRTAVSSFGDISFAFKPTMEQALRLIA
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHH
AVPSIIAFRKRLADGEEPVQPLDELGFVENYFYMLKGKRPTEAQKKALETYMILAMEHGM
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHCCC
NASTFSARVTVSTESDLVSAVTSALGTMKGPLHGGAPSAVTDMLEGIGEKQHAESYIREK
CCCCEEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHH
LEKGERLMGFGHRVYKTHDPRAKALRVKAEEIAGGDRDIDLALHVENTAIRLLEEYKPGR
HHHCCHHHHCCHHHHCCCCCHHHHHEEEHHHHCCCCCCEEEEEEECHHHHHHHHHCCCCC
KLYTNVEFYAAAVMKAIDFDAALFTPTFSAARMVGWCAHVLEQADNNMIFRPSAKYVGEL
HHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHH
VSQ
HCC
>Mature Secondary Structure
MVYHGLKGITCVETAISRIDGEKGKLIYRGYNADELALSSTFEEVAYLILNGAFPTEAEL
CCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCHHHH
SRFNELLASFRVLPEACERLIRSLPAELDDMAVLRTAVSSFGDISFAFKPTMEQALRLIA
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHH
AVPSIIAFRKRLADGEEPVQPLDELGFVENYFYMLKGKRPTEAQKKALETYMILAMEHGM
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHCCC
NASTFSARVTVSTESDLVSAVTSALGTMKGPLHGGAPSAVTDMLEGIGEKQHAESYIREK
CCCCEEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHH
LEKGERLMGFGHRVYKTHDPRAKALRVKAEEIAGGDRDIDLALHVENTAIRLLEEYKPGR
HHHCCHHHHCCHHHHCCCCCHHHHHEEEHHHHCCCCCCEEEEEEECHHHHHHHHHCCCCC
KLYTNVEFYAAAVMKAIDFDAALFTPTFSAARMVGWCAHVLEQADNNMIFRPSAKYVGEL
HHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHH
VSQ
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8045898; 9579061; 9384377 [H]