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The map label for this gene is glpK

Identifier: 52784767

GI number: 52784767

Start: 997397

End: 998887

Strand: Direct

Name: glpK

Synonym: BLi00995

Alternate gene names: 52784767

Gene position: 997397-998887 (Clockwise)

Preceding gene: 52784766

Following gene: 52784768

Centisome position: 23.62

GC content: 48.83

Gene sequence:

>1491_bases
ATGGAAAAGTACATTTTGTCTCTTGATCAAGGCACCACAAGCACAAGGGCGATTGTTTTCAACAAAGCAGGCGAAATCGT
CCATATTGCGCAAAAGGAATTCCGGCAATATTTTCCAAACCCTGGCTGGGTTGAACACAATGCAAACGAAATCTGGGGCT
CTGTTCTGTCGGTGATCGCTTCAGCGCTTTCAGAATCGGGGATCGAAGCCGGACAAATTGCCGGAATCGGGATCACAAAC
CAGCGGGAAACGACCGTGGTTTGGGATAAACATACCGGCAAACCGGTCTACAACGCGATTGTGTGGCAGTCCCGCCAATC
GGCTGAGATATGCCAGGAATTAAAAGAGAAAGGCTATGAAGAGACGATCAGAGAAAAAACAGGGCTTTTAATCGATCCTT
ATTTTTCAGGCACGAAAGTGAAATGGATCCTGGATCATGTGGAAGGGGCAAGGGAGAAAGCCGAAAACGGCGACCTTCTC
TTCGGTACGATCGATTCTTGGCTGATCTGGAAAATGTCCGGCGGAAAAGCGCATGTGACAGATTATTCAAACGCCTCAAG
AACATTGATGTTCAACATCTATGACCTAAAATGGGATGATGAACTTCTCGATATTCTCGGCGTGCCGAAATCGATGCTTC
CGGAAGTCAAGCCTTCATCGCATGTATACGCTGAAACGGTCGATTATCATTTCTTCGGCAAAAACATTCCGATTGCAGGT
GCAGCCGGCGACCAGCAGGCAGCATTGTTCGGGCAGGCTTGCTTTGAAGAAGGAATGGTTAAGAACACGTATGGAACAGG
CTGCTTTATGCTGATGAACACCGGCGAGAAAGCGATTAAATCAGAGCACGGCCTGCTGACGACAATCGCTTGGGGCATCG
ACGGAAAGGTGGAATATGCGCTGGAAGGCAGCGTCTTCGTCGCGGGTTCCGCTATTCAATGGCTGCGTGATGGGCTGAGA
ATGTTTAAAGACGCCAAAGAAAGTGAAAAATACGCTGTAAGAGCAGAATCTGCCGATGGTGTTTATGTGGTCCCTGCATT
TGTAGGTTTAGGCACGCCTTATTGGGACAGCGATGTCCGCGGCGCTGTATTCGGACTGACCCGGGGTACGACGAAAGAGC
ATTTTATCAGAGCAACGCTTGAAGCGCTTGCCTATCAAACGAAAGACGTGCTGGACGCAATGAAGGAAGACTCCGGGATC
CCGGTTAAAACGCTGAGAGTCGACGGCGGAGCTGTCAAAAACAACTTCCTGATGGATTTTCAGGGCGACATTTTAGATGT
CCCTGTAGAACGTCCTGAAATCAATGAAACAACAGCGCTTGGTTCAGCCTATTTAGCGGGCCTTGCTGTCGGCTTCTGGA
GCGATCGTTCCGAGATCAAAGACCAGTGGCAGCTTGACAAACGTTTTGAACCGAAAATGGAAGAAAAAGAGCGTGAGAGC
CTGTACAACGGGTGGAAGAAAGCTGTAAATGCAGCTAGGGCTTTTAAATAA

Upstream 100 bases:

>100_bases
GTTAGGTTTCTATATTCATATGAAAAAACAAGCAGTTGATCAATCGGTCAACATTTAAAAAAAACGCAATCTTAACAGAC
ATATAAGGGGGAGTTTCAAA

Downstream 100 bases:

>100_bases
GCTGCATGTATGTTACAATCTAATTAAGTTAATAGAAACGGTTGGAGAAGAGGAGAGACCGCAGACACCAAAGCAGTATC
AGCGCTTTGGATGTTTGTGG

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MEKYILSLDQGTTSTRAIVFNKAGEIVHIAQKEFRQYFPNPGWVEHNANEIWGSVLSVIASALSESGIEAGQIAGIGITN
QRETTVVWDKHTGKPVYNAIVWQSRQSAEICQELKEKGYEETIREKTGLLIDPYFSGTKVKWILDHVEGAREKAENGDLL
FGTIDSWLIWKMSGGKAHVTDYSNASRTLMFNIYDLKWDDELLDILGVPKSMLPEVKPSSHVYAETVDYHFFGKNIPIAG
AAGDQQAALFGQACFEEGMVKNTYGTGCFMLMNTGEKAIKSEHGLLTTIAWGIDGKVEYALEGSVFVAGSAIQWLRDGLR
MFKDAKESEKYAVRAESADGVYVVPAFVGLGTPYWDSDVRGAVFGLTRGTTKEHFIRATLEALAYQTKDVLDAMKEDSGI
PVKTLRVDGGAVKNNFLMDFQGDILDVPVERPEINETTALGSAYLAGLAVGFWSDRSEIKDQWQLDKRFEPKMEEKERES
LYNGWKKAVNAARAFK

Sequences:

>Translated_496_residues
MEKYILSLDQGTTSTRAIVFNKAGEIVHIAQKEFRQYFPNPGWVEHNANEIWGSVLSVIASALSESGIEAGQIAGIGITN
QRETTVVWDKHTGKPVYNAIVWQSRQSAEICQELKEKGYEETIREKTGLLIDPYFSGTKVKWILDHVEGAREKAENGDLL
FGTIDSWLIWKMSGGKAHVTDYSNASRTLMFNIYDLKWDDELLDILGVPKSMLPEVKPSSHVYAETVDYHFFGKNIPIAG
AAGDQQAALFGQACFEEGMVKNTYGTGCFMLMNTGEKAIKSEHGLLTTIAWGIDGKVEYALEGSVFVAGSAIQWLRDGLR
MFKDAKESEKYAVRAESADGVYVVPAFVGLGTPYWDSDVRGAVFGLTRGTTKEHFIRATLEALAYQTKDVLDAMKEDSGI
PVKTLRVDGGAVKNNFLMDFQGDILDVPVERPEINETTALGSAYLAGLAVGFWSDRSEIKDQWQLDKRFEPKMEEKERES
LYNGWKKAVNAARAFK
>Mature_496_residues
MEKYILSLDQGTTSTRAIVFNKAGEIVHIAQKEFRQYFPNPGWVEHNANEIWGSVLSVIASALSESGIEAGQIAGIGITN
QRETTVVWDKHTGKPVYNAIVWQSRQSAEICQELKEKGYEETIREKTGLLIDPYFSGTKVKWILDHVEGAREKAENGDLL
FGTIDSWLIWKMSGGKAHVTDYSNASRTLMFNIYDLKWDDELLDILGVPKSMLPEVKPSSHVYAETVDYHFFGKNIPIAG
AAGDQQAALFGQACFEEGMVKNTYGTGCFMLMNTGEKAIKSEHGLLTTIAWGIDGKVEYALEGSVFVAGSAIQWLRDGLR
MFKDAKESEKYAVRAESADGVYVVPAFVGLGTPYWDSDVRGAVFGLTRGTTKEHFIRATLEALAYQTKDVLDAMKEDSGI
PVKTLRVDGGAVKNNFLMDFQGDILDVPVERPEINETTALGSAYLAGLAVGFWSDRSEIKDQWQLDKRFEPKMEEKERES
LYNGWKKAVNAARAFK

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family

Homologues:

Organism=Homo sapiens, GI189409120, Length=496, Percent_Identity=46.5725806451613, Blast_Score=437, Evalue=1e-123,
Organism=Homo sapiens, GI42794763, Length=500, Percent_Identity=46.2, Blast_Score=437, Evalue=1e-123,
Organism=Homo sapiens, GI4504007, Length=496, Percent_Identity=46.5725806451613, Blast_Score=437, Evalue=1e-122,
Organism=Homo sapiens, GI41393575, Length=493, Percent_Identity=45.841784989858, Blast_Score=431, Evalue=1e-121,
Organism=Homo sapiens, GI88196792, Length=502, Percent_Identity=35.0597609561753, Blast_Score=335, Evalue=5e-92,
Organism=Escherichia coli, GI1790361, Length=495, Percent_Identity=62.020202020202, Blast_Score=668, Evalue=0.0,
Organism=Escherichia coli, GI1790005, Length=509, Percent_Identity=22.9862475442043, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1787789, Length=513, Percent_Identity=23.0019493177388, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1789168, Length=491, Percent_Identity=23.2179226069246, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789987, Length=488, Percent_Identity=22.3360655737705, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1789138, Length=477, Percent_Identity=21.5932914046122, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17535599, Length=499, Percent_Identity=41.2825651302605, Blast_Score=384, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI71986140, Length=520, Percent_Identity=25.3846153846154, Blast_Score=194, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI71986147, Length=299, Percent_Identity=29.4314381270903, Blast_Score=140, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6321755, Length=511, Percent_Identity=36.399217221135, Blast_Score=308, Evalue=9e-85,
Organism=Drosophila melanogaster, GI17864214, Length=511, Percent_Identity=40.9001956947162, Blast_Score=388, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24654573, Length=438, Percent_Identity=42.0091324200913, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24655555, Length=460, Percent_Identity=38.2608695652174, Blast_Score=360, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24655560, Length=460, Percent_Identity=38.2608695652174, Blast_Score=360, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24655570, Length=460, Percent_Identity=38.2608695652174, Blast_Score=360, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24655574, Length=460, Percent_Identity=38.2608695652174, Blast_Score=360, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24655565, Length=460, Percent_Identity=38.2608695652174, Blast_Score=360, Evalue=1e-100,
Organism=Drosophila melanogaster, GI19922058, Length=522, Percent_Identity=31.6091954022989, Blast_Score=268, Evalue=5e-72,
Organism=Drosophila melanogaster, GI24652871, Length=489, Percent_Identity=32.1063394683027, Blast_Score=254, Evalue=9e-68,
Organism=Drosophila melanogaster, GI24656367, Length=501, Percent_Identity=26.5469061876248, Blast_Score=219, Evalue=5e-57,
Organism=Drosophila melanogaster, GI21357867, Length=516, Percent_Identity=25.7751937984496, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI24656372, Length=330, Percent_Identity=28.4848484848485, Blast_Score=172, Evalue=4e-43,
Organism=Drosophila melanogaster, GI45552909, Length=290, Percent_Identity=25.8620689655172, Blast_Score=127, Evalue=1e-29,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GLPK_BACLD (Q65M11)

Other databases:

- EMBL:   CP000002
- EMBL:   AE017333
- RefSeq:   YP_078198.1
- RefSeq:   YP_090596.1
- HSSP:   O34153
- ProteinModelPortal:   Q65M11
- SMR:   Q65M11
- STRING:   Q65M11
- EnsemblBacteria:   EBBACT00000056110
- EnsemblBacteria:   EBBACT00000061992
- GeneID:   3029876
- GeneID:   3098715
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi00995
- KEGG:   bli:BL02903
- NMPDR:   fig|279010.5.peg.1669
- eggNOG:   COG0554
- GeneTree:   EBGT00050000001374
- HOGENOM:   HBG511469
- OMA:   KPSSEVY
- ProtClustDB:   PRK00047
- BioCyc:   BLIC279010-1:BLI00995-MONOMER
- BioCyc:   BLIC279010:BL02903-MONOMER
- HAMAP:   MF_00186
- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999
- PANTHER:   PTHR10196
- PANTHER:   PTHR10196:SF9
- TIGRFAMs:   TIGR01311

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N

EC number: =2.7.1.30

Molecular weight: Translated: 55183; Mature: 55183

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS00933 FGGY_KINASES_1; PS00445 FGGY_KINASES_2

Important sites: BINDING 12-12 BINDING 16-16 BINDING 82-82 BINDING 134-134 BINDING 244-244 BINDING 266-266 BINDING 309-309

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKYILSLDQGTTSTRAIVFNKAGEIVHIAQKEFRQYFPNPGWVEHNANEIWGSVLSVIA
CCCEEEEECCCCCCCEEEEEECCCCEEEHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
SALSESGIEAGQIAGIGITNQRETTVVWDKHTGKPVYNAIVWQSRQSAEICQELKEKGYE
HHHHHCCCCCCCEEEECCCCCCCEEEEEECCCCCHHHHHHHCCCCCHHHHHHHHHHCCHH
ETIREKTGLLIDPYFSGTKVKWILDHVEGAREKAENGDLLFGTIDSWLIWKMSGGKAHVT
HHHHHHCCCEEECCCCCCEEEHHHHHHHHHHHHCCCCCEEEEECCCEEEEEECCCEEEEE
DYSNASRTLMFNIYDLKWDDELLDILGVPKSMLPEVKPSSHVYAETVDYHFFGKNIPIAG
ECCCCCEEEEEEEEECCCCHHHHHHHCCCHHHCCCCCCCCCEEEEEEEEEEECCCCCEEC
AAGDQQAALFGQACFEEGMVKNTYGTGCFMLMNTGEKAIKSEHGLLTTIAWGIDGKVEYA
CCCCCHHHHHHHHHHHCCCEECCCCCEEEEEEECCHHHHHCCCCEEEEEEECCCCEEEEE
LEGSVFVAGSAIQWLRDGLRMFKDAKESEKYAVRAESADGVYVVPAFVGLGTPYWDSDVR
ECCCEEEECHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEEHHHHCCCCCCCCCCC
GAVFGLTRGTTKEHFIRATLEALAYQTKDVLDAMKEDSGIPVKTLRVDGGAVKNNFLMDF
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEECCCEEEEE
QGDILDVPVERPEINETTALGSAYLAGLAVGFWSDRSEIKDQWQLDKRFEPKMEEKERES
CCCEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHH
LYNGWKKAVNAARAFK
HHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEKYILSLDQGTTSTRAIVFNKAGEIVHIAQKEFRQYFPNPGWVEHNANEIWGSVLSVIA
CCCEEEEECCCCCCCEEEEEECCCCEEEHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
SALSESGIEAGQIAGIGITNQRETTVVWDKHTGKPVYNAIVWQSRQSAEICQELKEKGYE
HHHHHCCCCCCCEEEECCCCCCCEEEEEECCCCCHHHHHHHCCCCCHHHHHHHHHHCCHH
ETIREKTGLLIDPYFSGTKVKWILDHVEGAREKAENGDLLFGTIDSWLIWKMSGGKAHVT
HHHHHHCCCEEECCCCCCEEEHHHHHHHHHHHHCCCCCEEEEECCCEEEEEECCCEEEEE
DYSNASRTLMFNIYDLKWDDELLDILGVPKSMLPEVKPSSHVYAETVDYHFFGKNIPIAG
ECCCCCEEEEEEEEECCCCHHHHHHHCCCHHHCCCCCCCCCEEEEEEEEEEECCCCCEEC
AAGDQQAALFGQACFEEGMVKNTYGTGCFMLMNTGEKAIKSEHGLLTTIAWGIDGKVEYA
CCCCCHHHHHHHHHHHCCCEECCCCCEEEEEEECCHHHHHCCCCEEEEEEECCCCEEEEE
LEGSVFVAGSAIQWLRDGLRMFKDAKESEKYAVRAESADGVYVVPAFVGLGTPYWDSDVR
ECCCEEEECHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEEHHHHCCCCCCCCCCC
GAVFGLTRGTTKEHFIRATLEALAYQTKDVLDAMKEDSGIPVKTLRVDGGAVKNNFLMDF
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEECCCEEEEE
QGDILDVPVERPEINETTALGSAYLAGLAVGFWSDRSEIKDQWQLDKRFEPKMEEKERES
CCCEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHH
LYNGWKKAVNAARAFK
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA