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The map label for this gene is thiC

Identifier: 52784713

GI number: 52784713

Start: 942291

End: 944066

Strand: Direct

Name: thiC

Synonym: BLi00940

Alternate gene names: 52784713

Gene position: 942291-944066 (Clockwise)

Preceding gene: 52784712

Following gene: 52784714

Centisome position: 22.32

GC content: 50.9

Gene sequence:

>1776_bases
ATGAAACAGGAATCAGTACAGCAAAAAAACATCTCGATAATGTCAAGTTTTTCAGGGAGCCGCAAAGTGTATGTCGAAGG
GTCGCGTCCTGACATTCAAGTGCCGATGAGAGAAATTTCGTTAAGTCCGACGACGGGTTCGTTTGGCGACGAAGAAAACC
AGCCTGTCCGCGTCTACGACACGAGCGGCCCCTATACGGACGCCAATGCGGACGTTGATATTCTTAAGGGGCTGAAACCT
TTGCGGGCGACCTGGATCAGGGAGCGCGGCGATACCGAAGAATATGAAGGCAGAGAAATAAGACCGGAGGATAACGGCTA
TAAAGGCAAAGAGAAAGCGAGCCACAGCTATACAGGGTTGAAACGAAAACCGCTGCGCGCGAAAAAAGGACAAAATGTCA
CTCAGCTGCATTACGCAAGAAAGGGGATCATTACGCCCGAGATGGAATTTATTGCGATCAGAGAGCACGTATCTCCCGAA
TTTGTTCGTGATGAAGTGGCAAGCGGACGGGCGATTATTCCGTCAAACATCAACCATCCGGAAAGCGAACCGATGATTAT
CGGCCGCAACTTCCACGTCAAAATCAATGCCAACATCGGCAATTCCGCTGTCACCTCTTCGATTGAAGAAGAAGTGGAAA
AAATGACATGGGCCATCCGCTGGGGCGCTGATACGATGATGGACTTGTCCACAGGTAAAGATATTCATACAACGAGAGAA
TGGATCATCCGCAACTGTCCGGTGCCTGTGGGAACAGTCCCGATTTATCAGGCGCTTGAAAAAGTAAACGGCGTCGCGGA
AGATCTTACTTGGGACATTTACCGCGATACATTGATCGAACAGGCTGAGCAAGGCGTTGATTATTTCACGATTCACGCCG
GCGTCCTTCTCCGGTATGTGCCGTTAACAGCAAAGCGCGTCACCGGAATCGTATCCCGCGGCGGGGCGATCATGGCGCAA
TGGTGTTTGGCTCATCATGAGGAAAGCTTTCTGTACACCCATTTTGAAGAGATATGCGAGATTATGAAAACATACGATAT
CGCATTTTCGCTCGGAGACGGTCTTCGTCCCGGCTCGATTGCCGATGCGAATGATGAGGCCCAATTTGCCGAATTGGAGA
CGCTCGGCGAGCTGACGGAAATCGCCTGGAAGCATGATGTTCAAGTAATGATCGAAGGCCCGGGACACGTGCCGATGCAT
AAAATCAAAGAGAATGTCGACAAGCAGATGGAGATTTGCAAAGAGGCACCGTTTTACACGCTCGGTCCGCTGACGACCGA
TATTGCACCGGGCTACGACCATATTACATCGGCGATCGGAGCGGCGATGATCGGCTGGTACGGAACGGCCATGCTTTGCT
ATGTGACGCCGAAGGAGCATTTGGGACTGCCGAACAAAGATGATGTCCGCGAAGGCGTCATAACATACAAAATCGCGGCC
CATGCGGCTGACCTTGCAAAAGGGCACCCCGGCGCGCAAATCCGCGATGACGCGCTTTCAAAGGCGCGCTTTGAATTCCG
CTGGCGGGATCAGTTCAACTTGTCGCTCGATCCGGAAAGAGCGCTCGAATACCATGATGAAACCCTTCCGGCGGAAGGCG
CCAAAACGGCACACTTTTGTTCAATGTGCGGGCCTAAATTTTGCAGCATGAGGATTTCCCAGGATATTCGCGACTACGCC
AAGGAAAACAACCTTGATGAAGAAAAGGCTATCCAAAAAGGATTGGAAGAGAAGGCGAAGGAATTTAAAAAAGCAGGCGG
CAGCATTTACAGTTAA

Upstream 100 bases:

>100_bases
TGACTGTAAAACCAGGTTCATCATATGAGCCTGGTTTTTTGTGCTGGTGCTGTTCCGGAGTTTCGTCCTGATCAATTTTT
TAGAGGGGAAGGATTTTTAC

Downstream 100 bases:

>100_bases
AAGCGGGCAATACAGCAGATGAAAGGGGAACCTTTCATCTGTTTTTTTATAATATAGGGTTGACAGGTCGGAATAATATG
AATTAGTATGTTATTTAATA

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 591; Mature: 591

Protein sequence:

>591_residues
MKQESVQQKNISIMSSFSGSRKVYVEGSRPDIQVPMREISLSPTTGSFGDEENQPVRVYDTSGPYTDANADVDILKGLKP
LRATWIRERGDTEEYEGREIRPEDNGYKGKEKASHSYTGLKRKPLRAKKGQNVTQLHYARKGIITPEMEFIAIREHVSPE
FVRDEVASGRAIIPSNINHPESEPMIIGRNFHVKINANIGNSAVTSSIEEEVEKMTWAIRWGADTMMDLSTGKDIHTTRE
WIIRNCPVPVGTVPIYQALEKVNGVAEDLTWDIYRDTLIEQAEQGVDYFTIHAGVLLRYVPLTAKRVTGIVSRGGAIMAQ
WCLAHHEESFLYTHFEEICEIMKTYDIAFSLGDGLRPGSIADANDEAQFAELETLGELTEIAWKHDVQVMIEGPGHVPMH
KIKENVDKQMEICKEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWYGTAMLCYVTPKEHLGLPNKDDVREGVITYKIAA
HAADLAKGHPGAQIRDDALSKARFEFRWRDQFNLSLDPERALEYHDETLPAEGAKTAHFCSMCGPKFCSMRISQDIRDYA
KENNLDEEKAIQKGLEEKAKEFKKAGGSIYS

Sequences:

>Translated_591_residues
MKQESVQQKNISIMSSFSGSRKVYVEGSRPDIQVPMREISLSPTTGSFGDEENQPVRVYDTSGPYTDANADVDILKGLKP
LRATWIRERGDTEEYEGREIRPEDNGYKGKEKASHSYTGLKRKPLRAKKGQNVTQLHYARKGIITPEMEFIAIREHVSPE
FVRDEVASGRAIIPSNINHPESEPMIIGRNFHVKINANIGNSAVTSSIEEEVEKMTWAIRWGADTMMDLSTGKDIHTTRE
WIIRNCPVPVGTVPIYQALEKVNGVAEDLTWDIYRDTLIEQAEQGVDYFTIHAGVLLRYVPLTAKRVTGIVSRGGAIMAQ
WCLAHHEESFLYTHFEEICEIMKTYDIAFSLGDGLRPGSIADANDEAQFAELETLGELTEIAWKHDVQVMIEGPGHVPMH
KIKENVDKQMEICKEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWYGTAMLCYVTPKEHLGLPNKDDVREGVITYKIAA
HAADLAKGHPGAQIRDDALSKARFEFRWRDQFNLSLDPERALEYHDETLPAEGAKTAHFCSMCGPKFCSMRISQDIRDYA
KENNLDEEKAIQKGLEEKAKEFKKAGGSIYS
>Mature_591_residues
MKQESVQQKNISIMSSFSGSRKVYVEGSRPDIQVPMREISLSPTTGSFGDEENQPVRVYDTSGPYTDANADVDILKGLKP
LRATWIRERGDTEEYEGREIRPEDNGYKGKEKASHSYTGLKRKPLRAKKGQNVTQLHYARKGIITPEMEFIAIREHVSPE
FVRDEVASGRAIIPSNINHPESEPMIIGRNFHVKINANIGNSAVTSSIEEEVEKMTWAIRWGADTMMDLSTGKDIHTTRE
WIIRNCPVPVGTVPIYQALEKVNGVAEDLTWDIYRDTLIEQAEQGVDYFTIHAGVLLRYVPLTAKRVTGIVSRGGAIMAQ
WCLAHHEESFLYTHFEEICEIMKTYDIAFSLGDGLRPGSIADANDEAQFAELETLGELTEIAWKHDVQVMIEGPGHVPMH
KIKENVDKQMEICKEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWYGTAMLCYVTPKEHLGLPNKDDVREGVITYKIAA
HAADLAKGHPGAQIRDDALSKARFEFRWRDQFNLSLDPERALEYHDETLPAEGAKTAHFCSMCGPKFCSMRISQDIRDYA
KENNLDEEKAIQKGLEEKAKEFKKAGGSIYS

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=613, Percent_Identity=66.394779771615, Blast_Score=842, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_BACLD (Q65M65)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_078141.2
- RefSeq:   YP_090542.1
- STRING:   Q65M65
- EnsemblBacteria:   EBBACT00000054991
- EnsemblBacteria:   EBBACT00000062394
- GeneID:   3029797
- GeneID:   3100432
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi00940
- KEGG:   bli:BL05082
- NMPDR:   fig|279010.5.peg.1525
- eggNOG:   COG0422
- GeneTree:   EBGT00050000001472
- HOGENOM:   HBG285234
- ProtClustDB:   PRK09284
- BioCyc:   BLIC279010-1:BLI00940-MONOMER
- BioCyc:   BLIC279010:BL05082-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 66287; Mature: 66287

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: NA

Important sites: BINDING 198-198 BINDING 227-227 BINDING 256-256 BINDING 292-292 BINDING 392-392 BINDING 419-419

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQESVQQKNISIMSSFSGSRKVYVEGSRPDIQVPMREISLSPTTGSFGDEENQPVRVYD
CCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHEECCCCCCCCCCCCCCCEEEEE
TSGPYTDANADVDILKGLKPLRATWIRERGDTEEYEGREIRPEDNGYKGKEKASHSYTGL
CCCCCCCCCCCHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCC
KRKPLRAKKGQNVTQLHYARKGIITPEMEFIAIREHVSPEFVRDEVASGRAIIPSNINHP
CCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCEECCCCCCCC
ESEPMIIGRNFHVKINANIGNSAVTSSIEEEVEKMTWAIRWGADTMMDLSTGKDIHTTRE
CCCCEEECCEEEEEEECCCCCHHHHHHHHHHHHHHHEEEEECCCCEEECCCCCCHHHHHH
WIIRNCPVPVGTVPIYQALEKVNGVAEDLTWDIYRDTLIEQAEQGVDYFTIHAGVLLRYV
HHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHH
PLTAKRVTGIVSRGGAIMAQWCLAHHEESFLYTHFEEICEIMKTYDIAFSLGDGLRPGSI
CHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCCCCC
ADANDEAQFAELETLGELTEIAWKHDVQVMIEGPGHVPMHKIKENVDKQMEICKEAPFYT
CCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEE
LGPLTTDIAPGYDHITSAIGAAMIGWYGTAMLCYVTPKEHLGLPNKDDVREGVITYKIAA
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCHHHCCCCCHHHHHCCCEEEEHHH
HAADLAKGHPGAQIRDDALSKARFEFRWRDQFNLSLDPERALEYHDETLPAEGAKTAHFC
HHHHHHCCCCCCCCCHHHHHHHHEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHH
SMCGPKFCSMRISQDIRDYAKENNLDEEKAIQKGLEEKAKEFKKAGGSIYS
HHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKQESVQQKNISIMSSFSGSRKVYVEGSRPDIQVPMREISLSPTTGSFGDEENQPVRVYD
CCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHEECCCCCCCCCCCCCCCEEEEE
TSGPYTDANADVDILKGLKPLRATWIRERGDTEEYEGREIRPEDNGYKGKEKASHSYTGL
CCCCCCCCCCCHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCC
KRKPLRAKKGQNVTQLHYARKGIITPEMEFIAIREHVSPEFVRDEVASGRAIIPSNINHP
CCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCEECCCCCCCC
ESEPMIIGRNFHVKINANIGNSAVTSSIEEEVEKMTWAIRWGADTMMDLSTGKDIHTTRE
CCCCEEECCEEEEEEECCCCCHHHHHHHHHHHHHHHEEEEECCCCEEECCCCCCHHHHHH
WIIRNCPVPVGTVPIYQALEKVNGVAEDLTWDIYRDTLIEQAEQGVDYFTIHAGVLLRYV
HHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHH
PLTAKRVTGIVSRGGAIMAQWCLAHHEESFLYTHFEEICEIMKTYDIAFSLGDGLRPGSI
CHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCCCCC
ADANDEAQFAELETLGELTEIAWKHDVQVMIEGPGHVPMHKIKENVDKQMEICKEAPFYT
CCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEE
LGPLTTDIAPGYDHITSAIGAAMIGWYGTAMLCYVTPKEHLGLPNKDDVREGVITYKIAA
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCHHHCCCCCHHHHHCCCEEEEHHH
HAADLAKGHPGAQIRDDALSKARFEFRWRDQFNLSLDPERALEYHDETLPAEGAKTAHFC
HHHHHHCCCCCCCCCHHHHHHHHEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHH
SMCGPKFCSMRISQDIRDYAKENNLDEEKAIQKGLEEKAKEFKKAGGSIYS
HHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA