Click here to switch to the map view.

The map label for this gene is sacY [H]

Identifier: 52784641

GI number: 52784641

Start: 860437

End: 861279

Strand: Direct

Name: sacY [H]

Synonym: BLi00843

Alternate gene names: 52784641

Gene position: 860437-861279 (Clockwise)

Preceding gene: 52784640

Following gene: 52784647

Centisome position: 20.38

GC content: 41.28

Gene sequence:

>843_bases
ATGAAAGTAAAAAAAATCTTAAACAACAATGCGCTCGTCGTAAAGGATCGCGGCGAGGAAAAGATCGTATTAGGGTCCGG
AATCGCATTTCAAAAAAAGAAGAATGATATTGTCGACAGATCTAAAATTGAAAAAATATTCGTGATGAAAGATTCATCAG
AATACCGCCAATTCGAGGAGATCTTAAAAACATTGCCGGAAGATCATATTCAGGTGGCGGAGGATATTATTTCTTATGCG
GAAAAAGAGCTCGGGATCAAAATCAATGAGCGAATCCATGTCGCCTTTTCAGATCATTTATCCTTCGCGATAGAACGGTT
AAGCAAGGGAATGGTCATTAAAAATCCGCTGCTTCAAGAAGTCAAAGTCCTCTACACGAAAGAATTTCAAATCGGCCGCT
GGGCAAAGGCTCTCATTAAAGAAAAGCTGAAGATCGATATCCCCGAGGATGAAGTCGCCAATATTGCGCTGCATATCCAC
ACAGCCAAAACAAACGCGGGAGATATGACAAAAACGCTCGATCTCACAACGATGATCAAGGATATTATCGAGATCATCGA
AAACAAACTCAACATTAAAATAAAGGATGAGACGATTTCGTATGAGAGGCTTGTCAACCATCTCCGTTTCGCCATTCAGC
ATATCGAGTCGGGAGAGACATTTTACGAAATGGATGGTGAGATGATCAGCCTCATTAAAAAGAAGTTTGGACAAGCGTTT
ATGTGCGCAGAAGAAATCGGGGCATTTGTGAAGCGGGAGTATGGCTTTGAATTTCCGGAAAAAGAGCTGTGCTACATTAC
CATGCATATTCAGAGATTTTATCAGCGGTCTGCTGTCGTGTAA

Upstream 100 bases:

>100_bases
GGTGATTTCAGCATTTATTGCGACTTTGTGTCTTGGCTTTAAAGATGAAAAAGAATAATTCCGTTTTCATTCATTAAAAC
AGGATGAAAGGAAGGCAGCT

Downstream 100 bases:

>100_bases
AGATGCCTGCGTTTAAGACAAAACGGAGCCCTGTTAACGGGCTCCGTTTTTGTTTAAAGCTTCAACTTCCGTTAAAACCG
ATTTATCGATTAGAAATCCT

Product: SacY

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MKVKKILNNNALVVKDRGEEKIVLGSGIAFQKKKNDIVDRSKIEKIFVMKDSSEYRQFEEILKTLPEDHIQVAEDIISYA
EKELGIKINERIHVAFSDHLSFAIERLSKGMVIKNPLLQEVKVLYTKEFQIGRWAKALIKEKLKIDIPEDEVANIALHIH
TAKTNAGDMTKTLDLTTMIKDIIEIIENKLNIKIKDETISYERLVNHLRFAIQHIESGETFYEMDGEMISLIKKKFGQAF
MCAEEIGAFVKREYGFEFPEKELCYITMHIQRFYQRSAVV

Sequences:

>Translated_280_residues
MKVKKILNNNALVVKDRGEEKIVLGSGIAFQKKKNDIVDRSKIEKIFVMKDSSEYRQFEEILKTLPEDHIQVAEDIISYA
EKELGIKINERIHVAFSDHLSFAIERLSKGMVIKNPLLQEVKVLYTKEFQIGRWAKALIKEKLKIDIPEDEVANIALHIH
TAKTNAGDMTKTLDLTTMIKDIIEIIENKLNIKIKDETISYERLVNHLRFAIQHIESGETFYEMDGEMISLIKKKFGQAF
MCAEEIGAFVKREYGFEFPEKELCYITMHIQRFYQRSAVV
>Mature_280_residues
MKVKKILNNNALVVKDRGEEKIVLGSGIAFQKKKNDIVDRSKIEKIFVMKDSSEYRQFEEILKTLPEDHIQVAEDIISYA
EKELGIKINERIHVAFSDHLSFAIERLSKGMVIKNPLLQEVKVLYTKEFQIGRWAKALIKEKLKIDIPEDEVANIALHIH
TAKTNAGDMTKTLDLTTMIKDIIEIIENKLNIKIKDETISYERLVNHLRFAIQHIESGETFYEMDGEMISLIKKKFGQAF
MCAEEIGAFVKREYGFEFPEKELCYITMHIQRFYQRSAVV

Specific function: In the presence of sucrose, SacY is activated and prevents premature termination of transcription by binding to a RNA-antiterminator (RAT) sequence (partially overlapping with the terminator sequence) located upstream of the sacB gene. Formation of the Sa

COG id: COG3711

COG function: function code K; Transcriptional antiterminator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PRD domains [H]

Homologues:

Organism=Escherichia coli, GI1790160, Length=273, Percent_Identity=32.6007326007326, Blast_Score=181, Evalue=7e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004341
- InterPro:   IPR011608
- InterPro:   IPR001550 [H]

Pfam domain/function: PF03123 CAT_RBD; PF00874 PRD [H]

EC number: NA

Molecular weight: Translated: 32499; Mature: 32499

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: PS00654 ANTITERMINATORS_BGLG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVKKILNNNALVVKDRGEEKIVLGSGIAFQKKKNDIVDRSKIEKIFVMKDSSEYRQFEE
CCCHHHHCCCEEEEEECCCCEEEEECCCEECCCCCCHHHHHHHCEEEEEECCHHHHHHHH
ILKTLPEDHIQVAEDIISYAEKELGIKINERIHVAFSDHLSFAIERLSKGMVIKNPLLQE
HHHHCCHHHHHHHHHHHHHHHHHCCEEECCEEEEEEHHHHHHHHHHHHCCCEECCHHHHH
VKVLYTKEFQIGRWAKALIKEKLKIDIPEDEVANIALHIHTAKTNAGDMTKTLDLTTMIK
HHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHCEEEEEEEECCCCCCCCHHHHHHHHHHH
DIIEIIENKLNIKIKDETISYERLVNHLRFAIQHIESGETFYEMDGEMISLIKKKFGQAF
HHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEHHCHHHHHHHHHHHCCHH
MCAEEIGAFVKREYGFEFPEKELCYITMHIQRFYQRSAVV
HHHHHHHHHHHHHCCCCCCCCHHEEEHHHHHHHHHHHCCC
>Mature Secondary Structure
MKVKKILNNNALVVKDRGEEKIVLGSGIAFQKKKNDIVDRSKIEKIFVMKDSSEYRQFEE
CCCHHHHCCCEEEEEECCCCEEEEECCCEECCCCCCHHHHHHHCEEEEEECCHHHHHHHH
ILKTLPEDHIQVAEDIISYAEKELGIKINERIHVAFSDHLSFAIERLSKGMVIKNPLLQE
HHHHCCHHHHHHHHHHHHHHHHHCCEEECCEEEEEEHHHHHHHHHHHHCCCEECCHHHHH
VKVLYTKEFQIGRWAKALIKEKLKIDIPEDEVANIALHIHTAKTNAGDMTKTLDLTTMIK
HHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHCEEEEEEEECCCCCCCCHHHHHHHHHHH
DIIEIIENKLNIKIKDETISYERLVNHLRFAIQHIESGETFYEMDGEMISLIKKKFGQAF
HHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEHHCHHHHHHHHHHHCCHH
MCAEEIGAFVKREYGFEFPEKELCYITMHIQRFYQRSAVV
HHHHHHHHHHHHHCCCCCCCCHHEEEHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2116367; 1806041; 7934828; 9384377; 8535520; 9202047; 9261875; 9305643 [H]