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The map label for this gene is acoA [H]

Identifier: 52784647

GI number: 52784647

Start: 867562

End: 868536

Strand: Direct

Name: acoA [H]

Synonym: BLi00849

Alternate gene names: 52784647

Gene position: 867562-868536 (Clockwise)

Preceding gene: 52784641

Following gene: 52784648

Centisome position: 20.55

GC content: 50.67

Gene sequence:

>975_bases
ATGGAAGAGGCACTGACAAAGGAAAAAGCAGTGTGGATGTATCAAAAAATGCTGGAAATCCGCTATTTCGAAGATCAGGT
TCATCAGCTGTTTGCTAAAGGAATACTGCCGGGTTTTGTCCATCTTTACGCCGGAGAAGAAGCGGTCGCTGCGGGCGTCT
GCGCTCATCTGAATGATGAAGACACCATCACGAGCACACATCGCGGCCATGGGCACTGCATTGCAAAAGGGTGCGACTTG
AAAGGCATGATGGCCGAGATATACGGAAAATCTGCAGGACTTTGTAAAGGAAAAGGCGGATCGATGCATATTGCAGACTT
TGACAAAGGGATGCTAGGCGCAAACGGAATCGTCGGCGGGGGATTTCCTTTGGCCTGCGGAGCAGCTCTCACTGCAAAGT
ATAAAAAGACTAAAAATGTAAGCGTTTGCTTTTTTGGCGACGGCGCCAACAACCAGGGGACATTTCACGAAGGGATCAAC
CTTGCCGCCATCTGGAAGCTCCCTGTCATATTTGTCGCCGAAAACAACGGGTATGGCGAAGCGACTCCGTTTTCCTATGC
GTCAAGCTGCGAGTCGATCGCAGATCGTGCGGCCGCTTATGGGATTCCGGGCGTTCGCGTTGATGGAAAAGATGCGGCCG
CGGTCTTCCAGGCTGCGGGCCGGGCGATAGAGCGTGCCAAAAACGGAGAAGGACCGACGCTCATTGAGTGTATGACATAC
CGGAACTACGGCCATTTTGAAGGAGATGCCCAGCGCTACAAAACAAATGAAGAAAAAGCGGTGCATCAGGAAGAAAAAGA
TGCCATTGCCGTGTTCAAAAGCGAACTGCTGGCAAAAGGGCTGATGAGTGAAGAAGAGCAGTCGCGGATTGAAAAAGAAG
TTGAAGAAGCGGTCGAGGAAGCGGTCGCATTCAGCGAAAAAAGCGCTTATCCGGAAGAAACCGAACTGCTGACGGATGTA
TATGTATCCTATTAA

Upstream 100 bases:

>100_bases
AAAGGATGTCCGCCTGAAAAGCGGCTTATGTTCGCTTCGGAGAGCTGGCACGATTTTTGCATCATAACGTGTGAGTAAAA
AAGATAGGAGGTTTTTTCAT

Downstream 100 bases:

>100_bases
TTCATGAAGGGGGAAGGAAACATGACGAGGGAAATCAGCATGTCAGCCGCATTGAATGAAGCGATCAAACTTGCGATGAG
AAGGGACGATCACGTCATTT

Product: hypothetical protein

Products: [dihydrolipoyllysine-residue acetyltransferase] ; $S-acetyldihydrolipoyllysine; CO2

Alternate protein names: Acetoin:DCPIP oxidoreductase-alpha; Ao:DCPIP OR; TPP-dependent acetoin dehydrogenase E1 subunit alpha [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MEEALTKEKAVWMYQKMLEIRYFEDQVHQLFAKGILPGFVHLYAGEEAVAAGVCAHLNDEDTITSTHRGHGHCIAKGCDL
KGMMAEIYGKSAGLCKGKGGSMHIADFDKGMLGANGIVGGGFPLACGAALTAKYKKTKNVSVCFFGDGANNQGTFHEGIN
LAAIWKLPVIFVAENNGYGEATPFSYASSCESIADRAAAYGIPGVRVDGKDAAAVFQAAGRAIERAKNGEGPTLIECMTY
RNYGHFEGDAQRYKTNEEKAVHQEEKDAIAVFKSELLAKGLMSEEEQSRIEKEVEEAVEEAVAFSEKSAYPEETELLTDV
YVSY

Sequences:

>Translated_324_residues
MEEALTKEKAVWMYQKMLEIRYFEDQVHQLFAKGILPGFVHLYAGEEAVAAGVCAHLNDEDTITSTHRGHGHCIAKGCDL
KGMMAEIYGKSAGLCKGKGGSMHIADFDKGMLGANGIVGGGFPLACGAALTAKYKKTKNVSVCFFGDGANNQGTFHEGIN
LAAIWKLPVIFVAENNGYGEATPFSYASSCESIADRAAAYGIPGVRVDGKDAAAVFQAAGRAIERAKNGEGPTLIECMTY
RNYGHFEGDAQRYKTNEEKAVHQEEKDAIAVFKSELLAKGLMSEEEQSRIEKEVEEAVEEAVAFSEKSAYPEETELLTDV
YVSY
>Mature_324_residues
MEEALTKEKAVWMYQKMLEIRYFEDQVHQLFAKGILPGFVHLYAGEEAVAAGVCAHLNDEDTITSTHRGHGHCIAKGCDL
KGMMAEIYGKSAGLCKGKGGSMHIADFDKGMLGANGIVGGGFPLACGAALTAKYKKTKNVSVCFFGDGANNQGTFHEGIN
LAAIWKLPVIFVAENNGYGEATPFSYASSCESIADRAAAYGIPGVRVDGKDAAAVFQAAGRAIERAKNGEGPTLIECMTY
RNYGHFEGDAQRYKTNEEKAVHQEEKDAIAVFKSELLAKGLMSEEEQSRIEKEVEEAVEEAVAFSEKSAYPEETELLTDV
YVSY

Specific function: Catalyzes the 2,6-dichlorophenolindophenol-dependent cleavage of acetoin into acetate and acetaldehyde. The alpha subunit is probably the catalytic subunit of the enzyme [H]

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4885543, Length=320, Percent_Identity=36.25, Blast_Score=214, Evalue=6e-56,
Organism=Homo sapiens, GI291084742, Length=321, Percent_Identity=37.6947040498442, Blast_Score=196, Evalue=2e-50,
Organism=Homo sapiens, GI4505685, Length=320, Percent_Identity=37.8125, Blast_Score=196, Evalue=2e-50,
Organism=Homo sapiens, GI291084744, Length=328, Percent_Identity=36.890243902439, Blast_Score=189, Evalue=3e-48,
Organism=Homo sapiens, GI291084757, Length=321, Percent_Identity=33.6448598130841, Blast_Score=156, Evalue=2e-38,
Organism=Homo sapiens, GI11386135, Length=319, Percent_Identity=31.6614420062696, Blast_Score=152, Evalue=3e-37,
Organism=Homo sapiens, GI258645172, Length=319, Percent_Identity=31.6614420062696, Blast_Score=149, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI32564172, Length=319, Percent_Identity=38.2445141065831, Blast_Score=217, Evalue=8e-57,
Organism=Caenorhabditis elegans, GI17536047, Length=319, Percent_Identity=38.2445141065831, Blast_Score=216, Evalue=9e-57,
Organism=Caenorhabditis elegans, GI86563355, Length=318, Percent_Identity=30.5031446540881, Blast_Score=148, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI86563357, Length=318, Percent_Identity=30.5031446540881, Blast_Score=148, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6321026, Length=320, Percent_Identity=36.5625, Blast_Score=201, Evalue=1e-52,
Organism=Drosophila melanogaster, GI24639740, Length=321, Percent_Identity=40.1869158878505, Blast_Score=216, Evalue=2e-56,
Organism=Drosophila melanogaster, GI24639744, Length=321, Percent_Identity=40.1869158878505, Blast_Score=216, Evalue=2e-56,
Organism=Drosophila melanogaster, GI28571106, Length=321, Percent_Identity=40.1869158878505, Blast_Score=216, Evalue=2e-56,
Organism=Drosophila melanogaster, GI24639746, Length=306, Percent_Identity=40.1960784313725, Blast_Score=205, Evalue=3e-53,
Organism=Drosophila melanogaster, GI24639748, Length=322, Percent_Identity=37.5776397515528, Blast_Score=205, Evalue=3e-53,
Organism=Drosophila melanogaster, GI21355903, Length=324, Percent_Identity=27.4691358024691, Blast_Score=119, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017 [H]

Pfam domain/function: PF00676 E1_dh [H]

EC number: 1.2.4.1

Molecular weight: Translated: 35100; Mature: 35100

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEALTKEKAVWMYQKMLEIRYFEDQVHQLFAKGILPGFVHLYAGEEAVAAGVCAHLNDE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHCCCCC
DTITSTHRGHGHCIAKGCDLKGMMAEIYGKSAGLCKGKGGSMHIADFDKGMLGANGIVGG
CCCCCCCCCCCEEEECCCCCHHHHHHHHCCCCCCEECCCCEEEEEECCCCCCCCCCCCCC
GFPLACGAALTAKYKKTKNVSVCFFGDGANNQGTFHEGINLAAIWKLPVIFVAENNGYGE
CCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHCCCEEEEEECCEEEEECCCCCCC
ATPFSYASSCESIADRAAAYGIPGVRVDGKDAAAVFQAAGRAIERAKNGEGPTLIECMTY
CCCCHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEC
RNYGHFEGDAQRYKTNEEKAVHQEEKDAIAVFKSELLAKGLMSEEEQSRIEKEVEEAVEE
CCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
AVAFSEKSAYPEETELLTDVYVSY
HHHHHHCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MEEALTKEKAVWMYQKMLEIRYFEDQVHQLFAKGILPGFVHLYAGEEAVAAGVCAHLNDE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHCCCCC
DTITSTHRGHGHCIAKGCDLKGMMAEIYGKSAGLCKGKGGSMHIADFDKGMLGANGIVGG
CCCCCCCCCCCEEEECCCCCHHHHHHHHCCCCCCEECCCCEEEEEECCCCCCCCCCCCCC
GFPLACGAALTAKYKKTKNVSVCFFGDGANNQGTFHEGINLAAIWKLPVIFVAENNGYGE
CCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHCCCEEEEEECCEEEEECCCCCCC
ATPFSYASSCESIADRAAAYGIPGVRVDGKDAAAVFQAAGRAIERAKNGEGPTLIECMTY
CCCCHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEC
RNYGHFEGDAQRYKTNEEKAVHQEEKDAIAVFKSELLAKGLMSEEEQSRIEKEVEEAVEE
CCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
AVAFSEKSAYPEETELLTDVYVSY
HHHHHHCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine

Specific reaction: pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S- $acetyldihydrolipoyllysine + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 10368162; 9384377 [H]