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The map label for this gene is opuE [H]

Identifier: 52784528

GI number: 52784528

Start: 733340

End: 734821

Strand: Reverse

Name: opuE [H]

Synonym: BLi00728

Alternate gene names: 52784528

Gene position: 734821-733340 (Counterclockwise)

Preceding gene: 52784534

Following gene: 52784527

Centisome position: 17.4

GC content: 49.66

Gene sequence:

>1482_bases
GTGAGTGTTGAAGTATTTATTTCGTTGGGGATTTATTTTGCCGCGATGCTCTTAATCGGCTGGTACTCCTATAGAAAGAC
ATCCAATTTGAATGACTACATGCTTGGCGGCAGAGGTCTTGGGCCTGCTGTTACGGCTTTATCGGCCGGCGCGTCTGACA
TGAGCGGCTGGATGCTGATGGGACTGCCCGGAGCCATGTATGTGACAGGACTTTCAAGCGCCTGGCTTGCCGTCGGTTTA
ACAATCGGAGCTTATGTGAACTATTTGGTGGTCGCTCCCCGGCTGCGCACATATACAGAAGTAGCAAACGATGCGATTAC
AATTCCGGACTTTTTCGAAAATCGGTTTAATGATACAACAAGAATCTTGCGCCTGGCATCAGCCATCGTCATCATGGTGT
TTTTCACATTGTACACGTCGGCGGGTATGGTGTCCGGAGGAAGACTGTTTGAAACGGCGTTTGGCCTCGATTATAAAATC
GGTCTTTTCTTAACGGCCGCAGTCGTAATTGCCTATACGCTGTTCGGAGGATTTTTGGCCGTCAGTCTGACAGACTTCGT
GCAAGGTTTGATTATGTTTATCGCTTTAGTGCTCGTGCCGATCGTTGCCTTTACTCAATTAGGCGGAATTACGCCGACTT
TCAATGTGATCGAAGAAATCAATCCAAGCTTGCTCGATTTCTTCAAAGGAACGACCGTCATTAGCATTATTTCATTTCTC
GCCTGGGGACTGGGCTATTTCGGTCAGCCGCATATTATCGTCCGCTTTATGGCGATTTCATCCGTAAAAGAACTGAAGCC
TGCGCGCCGCATCGCGATGGGCTGGATGATCATCTCCGTAATCGGCGCTCTGCTCGTCGGACTGACCGGTGTGGCGTATG
TCCATGAGACGGGTACAGCGCTTAAAGATCCGGAAACGATTTTTATCGTGTTTTCAAAGGTATTATTCCATCCGCTCATC
ACCGGATTTTTGCTTTCCGCTTTGCTCGCGGCGATTATGAGTACGGTGTCTTCCCAGCTGCTTGTTACATCAAGCGCCAT
GACGGAAGATTTTTACCGGACGTTTTTCCGGCGCAAAGCTTCGGATAAGGAACTCGTTCTTGTCGGACGTCTGAGCGTTC
TGCTTGTCGCCGTCATCGCGATCCTTCTGTCTCTGACACCGAATAACACGATCCTTGATCTGGTCGGCTATGCGTGGGCC
GGGTTTGGTTCTGCATTCGGACCCGCCATCCTTCTCAGCTTGTACTGGAAGCGGATGAACCAGTGGGGCGCATTGGCCGG
GATGATTACAGGGGCCGTGACCGTGCTGATTTGGATTCTGACCGGCCTGCAGGAGAAGACGGGTATCTATGAAATGATCC
CTGGTTTCTTCCTGAGCTTGATTGTCGTTTACGCTGTCAGCATGATCACGAACAAACCGGCAAAAGAGGCAAGAGATATG
TTTGAAGAAATGGAACAGACGCATGAGGAAGAACATTCTTAA

Upstream 100 bases:

>100_bases
AGAGGAGGCCCTGCAATTTTCTTCGTACCCGCAAATCAGTATTGTTGAAAATTTTAAAGAGGTGATGCCACATTATTTTT
AAAGAGGAGAGATATGAAAC

Downstream 100 bases:

>100_bases
ATGATCAGGTTACGAAAGCCGCCGGGCGATATCCGGCGGCTTTTTTGCTCAGACGGAATTCTTTTGATGCCACGCCGGCA
ATATAATGATAGGAGTATAG

Product: OpuE

Products: NA

Alternate protein names: Sodium/proline symporter [H]

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MSVEVFISLGIYFAAMLLIGWYSYRKTSNLNDYMLGGRGLGPAVTALSAGASDMSGWMLMGLPGAMYVTGLSSAWLAVGL
TIGAYVNYLVVAPRLRTYTEVANDAITIPDFFENRFNDTTRILRLASAIVIMVFFTLYTSAGMVSGGRLFETAFGLDYKI
GLFLTAAVVIAYTLFGGFLAVSLTDFVQGLIMFIALVLVPIVAFTQLGGITPTFNVIEEINPSLLDFFKGTTVISIISFL
AWGLGYFGQPHIIVRFMAISSVKELKPARRIAMGWMIISVIGALLVGLTGVAYVHETGTALKDPETIFIVFSKVLFHPLI
TGFLLSALLAAIMSTVSSQLLVTSSAMTEDFYRTFFRRKASDKELVLVGRLSVLLVAVIAILLSLTPNNTILDLVGYAWA
GFGSAFGPAILLSLYWKRMNQWGALAGMITGAVTVLIWILTGLQEKTGIYEMIPGFFLSLIVVYAVSMITNKPAKEARDM
FEEMEQTHEEEHS

Sequences:

>Translated_493_residues
MSVEVFISLGIYFAAMLLIGWYSYRKTSNLNDYMLGGRGLGPAVTALSAGASDMSGWMLMGLPGAMYVTGLSSAWLAVGL
TIGAYVNYLVVAPRLRTYTEVANDAITIPDFFENRFNDTTRILRLASAIVIMVFFTLYTSAGMVSGGRLFETAFGLDYKI
GLFLTAAVVIAYTLFGGFLAVSLTDFVQGLIMFIALVLVPIVAFTQLGGITPTFNVIEEINPSLLDFFKGTTVISIISFL
AWGLGYFGQPHIIVRFMAISSVKELKPARRIAMGWMIISVIGALLVGLTGVAYVHETGTALKDPETIFIVFSKVLFHPLI
TGFLLSALLAAIMSTVSSQLLVTSSAMTEDFYRTFFRRKASDKELVLVGRLSVLLVAVIAILLSLTPNNTILDLVGYAWA
GFGSAFGPAILLSLYWKRMNQWGALAGMITGAVTVLIWILTGLQEKTGIYEMIPGFFLSLIVVYAVSMITNKPAKEARDM
FEEMEQTHEEEHS
>Mature_492_residues
SVEVFISLGIYFAAMLLIGWYSYRKTSNLNDYMLGGRGLGPAVTALSAGASDMSGWMLMGLPGAMYVTGLSSAWLAVGLT
IGAYVNYLVVAPRLRTYTEVANDAITIPDFFENRFNDTTRILRLASAIVIMVFFTLYTSAGMVSGGRLFETAFGLDYKIG
LFLTAAVVIAYTLFGGFLAVSLTDFVQGLIMFIALVLVPIVAFTQLGGITPTFNVIEEINPSLLDFFKGTTVISIISFLA
WGLGYFGQPHIIVRFMAISSVKELKPARRIAMGWMIISVIGALLVGLTGVAYVHETGTALKDPETIFIVFSKVLFHPLIT
GFLLSALLAAIMSTVSSQLLVTSSAMTEDFYRTFFRRKASDKELVLVGRLSVLLVAVIAILLSLTPNNTILDLVGYAWAG
FGSAFGPAILLSLYWKRMNQWGALAGMITGAVTVLIWILTGLQEKTGIYEMIPGFFLSLIVVYAVSMITNKPAKEARDMF
EEMEQTHEEEHS

Specific function: Catalyzes the sodium-dependent uptake of extracellular proline [H]

COG id: COG0591

COG function: function code ER; Na+/proline symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family [H]

Homologues:

Organism=Homo sapiens, GI310128183, Length=492, Percent_Identity=59.1463414634146, Blast_Score=588, Evalue=1e-168,
Organism=Homo sapiens, GI14140236, Length=558, Percent_Identity=23.8351254480287, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI11141885, Length=530, Percent_Identity=23.9622641509434, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI110835708, Length=549, Percent_Identity=21.8579234972678, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI157671931, Length=459, Percent_Identity=23.7472766884532, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1787251, Length=494, Percent_Identity=49.5951417004049, Blast_Score=486, Evalue=1e-138,
Organism=Escherichia coli, GI87082237, Length=447, Percent_Identity=29.9776286353468, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI1790503, Length=442, Percent_Identity=26.9230769230769, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI1790113, Length=389, Percent_Identity=24.1645244215938, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17539284, Length=460, Percent_Identity=24.7826086956522, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI115533094, Length=408, Percent_Identity=22.5490196078431, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24648033, Length=394, Percent_Identity=22.5888324873096, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI21356865, Length=394, Percent_Identity=22.5888324873096, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI221459588, Length=384, Percent_Identity=23.4375, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI221459584, Length=357, Percent_Identity=23.249299719888, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI221459586, Length=383, Percent_Identity=22.7154046997389, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24640370, Length=387, Percent_Identity=24.5478036175711, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI221459582, Length=380, Percent_Identity=24.2105263157895, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24651739, Length=477, Percent_Identity=22.8511530398323, Blast_Score=67, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24645928, Length=398, Percent_Identity=23.6180904522613, Blast_Score=66, Evalue=5e-11,
Organism=Drosophila melanogaster, GI28573698, Length=445, Percent_Identity=24.0449438202247, Blast_Score=66, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011851
- InterPro:   IPR001734
- InterPro:   IPR018212
- InterPro:   IPR019900 [H]

Pfam domain/function: PF00474 SSF [H]

EC number: NA

Molecular weight: Translated: 53763; Mature: 53632

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00456 NA_SOLUT_SYMP_1 ; PS00457 NA_SOLUT_SYMP_2 ; PS50283 NA_SOLUT_SYMP_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHCCC
>Mature Secondary Structure 
SVEVFISLGIYFAAMLLIGWYSYRKTSNLNDYMLGGRGLGPAVTALSAGASDMSGWMLM
CEEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCHHHHHHHCCCCCCCCEEEE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HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969499; 9384377 [H]