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The map label for this gene is prpB [C]

Identifier: 52784289

GI number: 52784289

Start: 472244

End: 473086

Strand: Reverse

Name: prpB [C]

Synonym: BLi00469

Alternate gene names: 52784289

Gene position: 473086-472244 (Counterclockwise)

Preceding gene: 52784290

Following gene: 52784288

Centisome position: 11.2

GC content: 51.96

Gene sequence:

>843_bases
ATGAAATCATTTGCAAAGTATCAAACATTTCTGGATCTCCATCATCAGTCAGACCCATTCGTTCTGCCAAATGCATGGGA
TGCAGCCAGCGCCAAAGCATTTCAAAACTGCGGTTTTAAAGCAGTCGGGACAACCAGCGCCGGAATCGCTATGTCCCTCG
GCTATCCCGACGGCGAACATCTCCCCTTTGAAACATTGCTTTCCGTACTGAAAACCATCACGGATGCCGTCGATATTCCC
GTCAGTGCAGATCTTGAAGCGGGTTACGGCCAAACACCGGAAACAGTCGCTGAAAACGCAAAAAAAGCCGCAGCTGCCGG
GGTTGTCGGTATGAATATTGAAGACGGAACGAAAGACCCCTGCCGGCCGCTTTCTGATGCAACATTAATGGAAGAAAAAA
TAACGGCTGTTAAAAAACTGAACCTTCCTGTTTTAATCAATGCCCGTACGGATGTGTACTGGCTCAATGCTGACGCCCCC
GGCTCCCGTCTTCAAGCAGCAGTCCGGCGGGCAAATCGGTACAGTCAAGCAGGCGCGGACTGCATTTTTATCCCCGGAGC
CGATGATGCAGGCACCATTGCCGGGCTGAGAAAAGGTATATCCGGGCCGATCAACGTGCTGGCCGGCTCACACACCCCCT
CTTTACAGATTCTTTCCGAACTGGGGATTGAAAGGATCAGCTGCGGATCCGCCCCTTTCAGAGCTGCACTGTCACTGGTG
AAAAAGATCGGTGAAGACATCATCCGCCGCGGCGCCTTCAATCGTATGACGGACGGCGTGCTTTCATACGGAGAAGCGGC
TGATTGGATCAGCCTTCATCGTGACCCAAAACAAAATCGTTGA

Upstream 100 bases:

>100_bases
CTATCGGTGATCGATAGCCTTTTTCCATGCGTTCCATTCCACATTTCGATGAGCATCGAAGGTCTTTACATTTACACTAT
GTAAAAAAGGAGTGGAATAC

Downstream 100 bases:

>100_bases
CCGAAACTGTTCGGTCTGTTAATATTTCATTTATGAAAACTCATGGGTTTTTTAGTGACCCATGAGTTTTATCATTGCGA
AAGAAGGAGCCGGAATGAAC

Product: hypothetical protein

Products: Pyruvate; Succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MKSFAKYQTFLDLHHQSDPFVLPNAWDAASAKAFQNCGFKAVGTTSAGIAMSLGYPDGEHLPFETLLSVLKTITDAVDIP
VSADLEAGYGQTPETVAENAKKAAAAGVVGMNIEDGTKDPCRPLSDATLMEEKITAVKKLNLPVLINARTDVYWLNADAP
GSRLQAAVRRANRYSQAGADCIFIPGADDAGTIAGLRKGISGPINVLAGSHTPSLQILSELGIERISCGSAPFRAALSLV
KKIGEDIIRRGAFNRMTDGVLSYGEAADWISLHRDPKQNR

Sequences:

>Translated_280_residues
MKSFAKYQTFLDLHHQSDPFVLPNAWDAASAKAFQNCGFKAVGTTSAGIAMSLGYPDGEHLPFETLLSVLKTITDAVDIP
VSADLEAGYGQTPETVAENAKKAAAAGVVGMNIEDGTKDPCRPLSDATLMEEKITAVKKLNLPVLINARTDVYWLNADAP
GSRLQAAVRRANRYSQAGADCIFIPGADDAGTIAGLRKGISGPINVLAGSHTPSLQILSELGIERISCGSAPFRAALSLV
KKIGEDIIRRGAFNRMTDGVLSYGEAADWISLHRDPKQNR
>Mature_280_residues
MKSFAKYQTFLDLHHQSDPFVLPNAWDAASAKAFQNCGFKAVGTTSAGIAMSLGYPDGEHLPFETLLSVLKTITDAVDIP
VSADLEAGYGQTPETVAENAKKAAAAGVVGMNIEDGTKDPCRPLSDATLMEEKITAVKKLNLPVLINARTDVYWLNADAP
GSRLQAAVRRANRYSQAGADCIFIPGADDAGTIAGLRKGISGPINVLAGSHTPSLQILSELGIERISCGSAPFRAALSLV
KKIGEDIIRRGAFNRMTDGVLSYGEAADWISLHRDPKQNR

Specific function: Catalyzes The Formation Of Pyruvate And Succinate From 2-Methylisocitrate (By Similarity). [C]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786525, Length=177, Percent_Identity=29.3785310734463, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR015813 [H]

Pfam domain/function: NA

EC number: 4.1.3.30 [C]

Molecular weight: Translated: 29811; Mature: 29811

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSFAKYQTFLDLHHQSDPFVLPNAWDAASAKAFQNCGFKAVGTTSAGIAMSLGYPDGEH
CCHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHCCCEEECCCCCCEEEEECCCCCCC
LPFETLLSVLKTITDAVDIPVSADLEAGYGQTPETVAENAKKAAAAGVVGMNIEDGTKDP
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCEEECCCCCCCCCC
CRPLSDATLMEEKITAVKKLNLPVLINARTDVYWLNADAPGSRLQAAVRRANRYSQAGAD
CCCCCHHHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCC
CIFIPGADDAGTIAGLRKGISGPINVLAGSHTPSLQILSELGIERISCGSAPFRAALSLV
EEEECCCCCCHHHHHHHHCCCCCCEEEECCCCCHHHHHHHCCCHHCCCCCCHHHHHHHHH
KKIGEDIIRRGAFNRMTDGVLSYGEAADWISLHRDPKQNR
HHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKSFAKYQTFLDLHHQSDPFVLPNAWDAASAKAFQNCGFKAVGTTSAGIAMSLGYPDGEH
CCHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHCCCEEECCCCCCEEEEECCCCCCC
LPFETLLSVLKTITDAVDIPVSADLEAGYGQTPETVAENAKKAAAAGVVGMNIEDGTKDP
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCEEECCCCCCCCCC
CRPLSDATLMEEKITAVKKLNLPVLINARTDVYWLNADAPGSRLQAAVRRANRYSQAGAD
CCCCCHHHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCC
CIFIPGADDAGTIAGLRKGISGPINVLAGSHTPSLQILSELGIERISCGSAPFRAALSLV
EEEECCCCCCHHHHHHHHCCCCCCEEEECCCCCHHHHHHHCCCHHCCCCCCHHHHHHHHH
KKIGEDIIRRGAFNRMTDGVLSYGEAADWISLHRDPKQNR
HHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: methylisocitrate [C]

Specific reaction: methylisocitrate <==> Pyruvate + Succinate [C]

General reaction: Elimination [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]