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The map label for this gene is sfp [H]

Identifier: 52784229

GI number: 52784229

Start: 407316

End: 407996

Strand: Reverse

Name: sfp [H]

Synonym: BLi00407

Alternate gene names: 52784229

Gene position: 407996-407316 (Counterclockwise)

Preceding gene: 52784232

Following gene: 52784227

Centisome position: 9.66

GC content: 49.63

Gene sequence:

>681_bases
ATGAAGATTTACGCCGTTTATATGGATCAGCTGCCGGAAGCCGGTCAATTTAACCGAATGATGGCCATGGTGTCTGAAGA
AAAGCGAAAAAAAGTGGGCAGATACAGATATCCCGAAGATGCCAGCCGGACGCTGATCGGAGAAGTGCTGGCGAGGCACA
TCATCAGCGAAACGTTTCATTTGCCGAATGAGCAGATCCGTTTTACAGAAGGAAGGTACGGCAAACCGGCTGCTGAAGGT
CTGAACGACTTTCATTTCAACATTTCCCACTCCGGAAAATGGATCGTGTGCGGAGCCGGCGCGCAGCCGATCGGAGTGGA
TGTCGAAAAGATCAAGCCGATCAATTTCGACATTGCCAAACGGTTTTTTTCACCTTCCGAGCACGATGATCTCATGGAAA
AGGACGAATCAGAACGCCTGTCGTATTTCTATCATTTGTGGACGATGAAAGAAAGCTTTATTAAACAGGCGGGAAAAGGC
CTGTCTCTGCCGCTTGATTCCTTCTCCGTCAAACTGAACGAACAGGGGCGAGCTTCGGTGGAAACTCCCCCCGGCTATCC
GCCTTGCTATATACAAGCCTATGAAATTGACCCCGGCTATAAAATGGCGGTGTGTTCCGCCGCACCTGAATTTCCCGGCC
GGATTGTGATGAAAAGCTGCGCTGAACTGTCAATGCTTTAA

Upstream 100 bases:

>100_bases
TGTTCATTACTCCATTCTGCACAAGTATCATGCCTATCTTTCCTGCTGTCTGTTTGCTAAAATGAAAGCAGAAAAATATC
ATGTGAAGAGGTTGCGTTTG

Downstream 100 bases:

>100_bases
AAAGTCCCGCCCTAAAAATAAGCGGTTTCCACCGCTTGCCGCAACCGCTCGATGCCGCTGCGGATGTCCTCTTCCTTCAC
ATTGGACGTATTGATTTTCA

Product: hypothetical protein

Products: adenosine 3',5'-bisphosphate [C]

Alternate protein names: Surfactin synthase-activating enzyme [H]

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MKIYAVYMDQLPEAGQFNRMMAMVSEEKRKKVGRYRYPEDASRTLIGEVLARHIISETFHLPNEQIRFTEGRYGKPAAEG
LNDFHFNISHSGKWIVCGAGAQPIGVDVEKIKPINFDIAKRFFSPSEHDDLMEKDESERLSYFYHLWTMKESFIKQAGKG
LSLPLDSFSVKLNEQGRASVETPPGYPPCYIQAYEIDPGYKMAVCSAAPEFPGRIVMKSCAELSML

Sequences:

>Translated_226_residues
MKIYAVYMDQLPEAGQFNRMMAMVSEEKRKKVGRYRYPEDASRTLIGEVLARHIISETFHLPNEQIRFTEGRYGKPAAEG
LNDFHFNISHSGKWIVCGAGAQPIGVDVEKIKPINFDIAKRFFSPSEHDDLMEKDESERLSYFYHLWTMKESFIKQAGKG
LSLPLDSFSVKLNEQGRASVETPPGYPPCYIQAYEIDPGYKMAVCSAAPEFPGRIVMKSCAELSML
>Mature_226_residues
MKIYAVYMDQLPEAGQFNRMMAMVSEEKRKKVGRYRYPEDASRTLIGEVLARHIISETFHLPNEQIRFTEGRYGKPAAEG
LNDFHFNISHSGKWIVCGAGAQPIGVDVEKIKPINFDIAKRFFSPSEHDDLMEKDESERLSYFYHLWTMKESFIKQAGKG
LSLPLDSFSVKLNEQGRASVETPPGYPPCYIQAYEIDPGYKMAVCSAAPEFPGRIVMKSCAELSML

Specific function: Activates the seven peptidyl carrier protein (PCP) domains of surfactin synthase SRF1/2/3 by transferring the 4'- phosphopantetheinyl moiety of coenzyme A (CoA) to a serine residue. Required for cells of B.subtilis to become producers of the lipopeptide a

COG id: COG2091

COG function: function code H; Phosphopantetheinyl transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P-Pant transferase superfamily. Gsp/sfp/hetI/acpT family [H]

Homologues:

Organism=Homo sapiens, GI20357568, Length=177, Percent_Identity=30.5084745762712, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17564880, Length=193, Percent_Identity=27.979274611399, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17569611, Length=173, Percent_Identity=27.7456647398844, Blast_Score=70, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24663173, Length=194, Percent_Identity=26.2886597938144, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008278
- InterPro:   IPR004568 [H]

Pfam domain/function: PF01648 ACPS [H]

EC number: NA

Molecular weight: Translated: 25675; Mature: 25675

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIYAVYMDQLPEAGQFNRMMAMVSEEKRKKVGRYRYPEDASRTLIGEVLARHIISETFH
CEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHC
LPNEQIRFTEGRYGKPAAEGLNDFHFNISHSGKWIVCGAGAQPIGVDVEKIKPINFDIAK
CCCCCEEECCCCCCCHHHCCCCCEEEEEECCCCEEEECCCCCCCCCCHHHCCCCCHHHHH
RFFSPSEHDDLMEKDESERLSYFYHLWTMKESFIKQAGKGLSLPLDSFSVKLNEQGRASV
HHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCC
ETPPGYPPCYIQAYEIDPGYKMAVCSAAPEFPGRIVMKSCAELSML
CCCCCCCCCEEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKIYAVYMDQLPEAGQFNRMMAMVSEEKRKKVGRYRYPEDASRTLIGEVLARHIISETFH
CEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHC
LPNEQIRFTEGRYGKPAAEGLNDFHFNISHSGKWIVCGAGAQPIGVDVEKIKPINFDIAK
CCCCCEEECCCCCCCHHHCCCCCEEEEEECCCCEEEECCCCCCCCCCHHHCCCCCHHHHH
RFFSPSEHDDLMEKDESERLSYFYHLWTMKESFIKQAGKGLSLPLDSFSVKLNEQGRASV
HHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCC
ETPPGYPPCYIQAYEIDPGYKMAVCSAAPEFPGRIVMKSCAELSML
CCCCCCCCCEEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Coenzyme A; apo-[acyl carrier protein] [C]

Specific reaction: Coenzyme A + apo-[acyl carrier protein] = adenosine 3',5'-bisphosphate + [acyl carrier protein] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1557038; 8407792; 8355609; 8639027; 9384377; 8939709; 9484229; 10581256 [H]