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The map label for this gene is ywbA [H]

Identifier: 52784156

GI number: 52784156

Start: 307965

End: 309251

Strand: Reverse

Name: ywbA [H]

Synonym: BLi00334

Alternate gene names: 52784156

Gene position: 309251-307965 (Counterclockwise)

Preceding gene: 52784157

Following gene: 52784155

Centisome position: 7.32

GC content: 48.33

Gene sequence:

>1287_bases
ATGAAACAAAATAAAATGATGGCATTCATCGAATCAAACATCATGCCGATCGCCGGAAGGATCGGTTCCCAAAGGCACCT
GATTGCAATACGCGACGGATTCATCGCCATTATGCCTCTTATCATCATCGGTTCCCTCGCCATCCTGGTCAATAATTTTC
CAATACCGGCGTTTCAAAACTTTATGACCGGTCTTTTCGGCGAGGGATGGACGGCTGTCGGCGGAGCGATATGGAATGGC
TCTTTTGCTCTGTTGAGCCTTTTAGCCGTTGCGGCTGTCAGCTTCAAGCTGGCCGAGTCATATCACACAGATGGTCTGTC
TGCCGCATTAATCGGGGTTGGCGCATTCGCCGTGCTTACACCGACCACTGAAGACTTTGGCTTCTCAATGACTTGGCTGG
GAGCCCAAGGTCTGTTCGTCGGCTTAATCGTGGCTTTGCTGTCGACAGAAGTCTTCCGGCTGTTTTCCAAAAATGAGAAA
TTGAAAATCAAAATGCCTGAAGGAGTGCCTGACGGCGTCACAAAATCCTTGAATGCTCTATTGCCGGCTGCCGTCATCTT
ATCCACTGCCGGGCTATGTAACGCTCTGATCGTTCACTTTTCGGGGCAAAGCATCCATGAGCTTGTTTTCAGCACGATTC
AAGAGCCGCTTCAAGGCCTTTCCAACACGGTTGGGTCAGCCTTGATTTTTACTTTCTTGAATCATTTGCTCTGGTTCTTT
GGATTGCATGGAACAAACATCCTCGGGCCGATCATGGAATCCGTCTACCTCCCGCTCATTCAGGAAAATCAGCACTTATT
CTCGCAAGGAATGAGCGCTTTTGAAGTGCCGTATATTGTGACAAAGCCTTTCTTCGATTCTTACGTATTTATGGGGGGAT
CGGGAACAACATTGGCTCTGCTTGCCGCCATTTTAATTGCCGTGAAATCGAAGCACTACAAAACGATAGGAAAGGTGTCT
CTCGCACCCGGCCTTTTTAACATCAATGAACCCGTTCTGTTCGGCCTTCCTATTGTATTAAACCCGATCATGTTCATTCC
GTTTATCTTGATTCCATTAATTTTGACGATTACATCATATATCGCTTTGGCTTCCGGTCTCGTCCCGAAAACTGTGGCGA
TCCTTCCGTGGACGACGCCTCCGATTGTCAGCGGATATCTTGTCACCGGAGGATCAGTCCGAGGGGTCTTTTTGCAAATC
TTCAACCTGGTGCTGGCCACCCTGCTCTACCTCCCGTTTATAAAAGCGGCTCAACGTTCCGCCTTGCACCAGACAGAAAA
TGAATAA

Upstream 100 bases:

>100_bases
CCATTGGTCTCTTCTGATCACAAAGCTCAAAAGGTGCTCGATGAAATGCTCGAGGCTCATAAACGTTATCTCCCGCAATT
TTTTGAGGAGGTCCGTGCAC

Downstream 100 bases:

>100_bases
ATAAGGAGTGAATGTTATGGCAGATACACTTCAATTGGAACAGGTCTCATTTGAAATCATCCTTCACGCCGGAAATGCGC
GTTCTTCTGCAATGGAAGCG

Product: YwbA

Products: pyruvate; diacetylchitobiose-6-phosphate [Cytoplasm]; cellobiose-6-phosphate [Cytoplasm] [C]

Alternate protein names: EIIC-Lic; PTS system lichenan-specific EIIC component [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MKQNKMMAFIESNIMPIAGRIGSQRHLIAIRDGFIAIMPLIIIGSLAILVNNFPIPAFQNFMTGLFGEGWTAVGGAIWNG
SFALLSLLAVAAVSFKLAESYHTDGLSAALIGVGAFAVLTPTTEDFGFSMTWLGAQGLFVGLIVALLSTEVFRLFSKNEK
LKIKMPEGVPDGVTKSLNALLPAAVILSTAGLCNALIVHFSGQSIHELVFSTIQEPLQGLSNTVGSALIFTFLNHLLWFF
GLHGTNILGPIMESVYLPLIQENQHLFSQGMSAFEVPYIVTKPFFDSYVFMGGSGTTLALLAAILIAVKSKHYKTIGKVS
LAPGLFNINEPVLFGLPIVLNPIMFIPFILIPLILTITSYIALASGLVPKTVAILPWTTPPIVSGYLVTGGSVRGVFLQI
FNLVLATLLYLPFIKAAQRSALHQTENE

Sequences:

>Translated_428_residues
MKQNKMMAFIESNIMPIAGRIGSQRHLIAIRDGFIAIMPLIIIGSLAILVNNFPIPAFQNFMTGLFGEGWTAVGGAIWNG
SFALLSLLAVAAVSFKLAESYHTDGLSAALIGVGAFAVLTPTTEDFGFSMTWLGAQGLFVGLIVALLSTEVFRLFSKNEK
LKIKMPEGVPDGVTKSLNALLPAAVILSTAGLCNALIVHFSGQSIHELVFSTIQEPLQGLSNTVGSALIFTFLNHLLWFF
GLHGTNILGPIMESVYLPLIQENQHLFSQGMSAFEVPYIVTKPFFDSYVFMGGSGTTLALLAAILIAVKSKHYKTIGKVS
LAPGLFNINEPVLFGLPIVLNPIMFIPFILIPLILTITSYIALASGLVPKTVAILPWTTPPIVSGYLVTGGSVRGVFLQI
FNLVLATLLYLPFIKAAQRSALHQTENE
>Mature_428_residues
MKQNKMMAFIESNIMPIAGRIGSQRHLIAIRDGFIAIMPLIIIGSLAILVNNFPIPAFQNFMTGLFGEGWTAVGGAIWNG
SFALLSLLAVAAVSFKLAESYHTDGLSAALIGVGAFAVLTPTTEDFGFSMTWLGAQGLFVGLIVALLSTEVFRLFSKNEK
LKIKMPEGVPDGVTKSLNALLPAAVILSTAGLCNALIVHFSGQSIHELVFSTIQEPLQGLSNTVGSALIFTFLNHLLWFF
GLHGTNILGPIMESVYLPLIQENQHLFSQGMSAFEVPYIVTKPFFDSYVFMGGSGTTLALLAAILIAVKSKHYKTIGKVS
LAPGLFNINEPVLFGLPIVLNPIMFIPFILIPLILTITSYIALASGLVPKTVAILPWTTPPIVSGYLVTGGSVRGVFLQI
FNLVLATLLYLPFIKAAQRSALHQTENE

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-3 domain [H]

Homologues:

Organism=Escherichia coli, GI1788032, Length=450, Percent_Identity=35.5555555555556, Blast_Score=273, Evalue=1e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003352
- InterPro:   IPR004796
- InterPro:   IPR004501 [H]

Pfam domain/function: PF02378 PTS_EIIC [H]

EC number: NA

Molecular weight: Translated: 46038; Mature: 46038

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: PS51105 PTS_EIIC_TYPE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQNKMMAFIESNIMPIAGRIGSQRHLIAIRDGFIAIMPLIIIGSLAILVNNFPIPAFQN
CCCCCEEHHHHCCCEEHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCHHHHH
FMTGLFGEGWTAVGGAIWNGSFALLSLLAVAAVSFKLAESYHTDGLSAALIGVGAFAVLT
HHHHHCCCCCHHHCCHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEEEC
PTTEDFGFSMTWLGAQGLFVGLIVALLSTEVFRLFSKNEKLKIKMPEGVPDGVTKSLNAL
CCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHH
LPAAVILSTAGLCNALIVHFSGQSIHELVFSTIQEPLQGLSNTVGSALIFTFLNHLLWFF
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLHGTNILGPIMESVYLPLIQENQHLFSQGMSAFEVPYIVTKPFFDSYVFMGGSGTTLAL
HCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCHHCCCEEEECCCCCCEEEECCCCHHHHH
LAAILIAVKSKHYKTIGKVSLAPGLFNINEPVLFGLPIVLNPIMFIPFILIPLILTITSY
HHHHHHHHHHHHHHHHHCEEECCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
IALASGLVPKTVAILPWTTPPIVSGYLVTGGSVRGVFLQIFNLVLATLLYLPFIKAAQRS
HHHHHCCCCCEEEEECCCCCCHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALHQTENE
HHHCCCCC
>Mature Secondary Structure
MKQNKMMAFIESNIMPIAGRIGSQRHLIAIRDGFIAIMPLIIIGSLAILVNNFPIPAFQN
CCCCCEEHHHHCCCEEHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCHHHHH
FMTGLFGEGWTAVGGAIWNGSFALLSLLAVAAVSFKLAESYHTDGLSAALIGVGAFAVLT
HHHHHCCCCCHHHCCHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEEEC
PTTEDFGFSMTWLGAQGLFVGLIVALLSTEVFRLFSKNEKLKIKMPEGVPDGVTKSLNAL
CCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHH
LPAAVILSTAGLCNALIVHFSGQSIHELVFSTIQEPLQGLSNTVGSALIFTFLNHLLWFF
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLHGTNILGPIMESVYLPLIQENQHLFSQGMSAFEVPYIVTKPFFDSYVFMGGSGTTLAL
HCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCHHCCCEEEECCCCCCEEEECCCCHHHHH
LAAILIAVKSKHYKTIGKVSLAPGLFNINEPVLFGLPIVLNPIMFIPFILIPLILTITSY
HHHHHHHHHHHHHHHHHCEEECCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
IALASGLVPKTVAILPWTTPPIVSGYLVTGGSVRGVFLQIFNLVLATLLYLPFIKAAQRS
HHHHHCCCCCEEEEECCCCCCHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALHQTENE
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: diacetylchitobiose [Periplasm]; phosphoenolpyruvate; cellobiose [Periplasm] [C]

Specific reaction: phosphoenolpyruvate + diacetylchitobiose [Periplasm] = pyruvate + diacetylchitobiose-6-phosphate [Cytoplasm] phosphoenolpyruvate + cellobiose [Periplasm] = cellobiose-6-phosphate [Cytoplasm] + pyruvate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8990303; 8969509; 9384377 [H]