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The map label for this gene is licH [H]

Identifier: 52784157

GI number: 52784157

Start: 309238

End: 310572

Strand: Reverse

Name: licH [H]

Synonym: BLi00335

Alternate gene names: 52784157

Gene position: 310572-309238 (Counterclockwise)

Preceding gene: 52784162

Following gene: 52784156

Centisome position: 7.35

GC content: 47.42

Gene sequence:

>1335_bases
ATGGCGCTGAAAGTAGTTGTTATAGGCGGAGGTTCCAGCTACACACCAGAAATCATTGAAGGCATCATCAAACGGCATCA
TCAATTTCCAGTGAATGAGATTGTACTGGTTGATATCGAGGAGGGCAAAGAAAAGCTTCGCATCGTCTCAGAACTCGCGA
AGCGAATGATCAGACATGCGGGGGTTCCCATCGAACTGAGCCATACAATAGATCGGGCAAAAGCATTGAAAGGCGCCGAT
TTCGTAACGGTTCAAATCAGAGTCGGCGGACTGAAGGCGAGAGCTTTAGACGAAAAAATTCCGCTCAGCCACGGCCTGAT
CGGCCAGGAAACAAACGGGGCGGGCGGCATGTTTAAAGCTTTGCGCACAATTCCCTTTATGCTGGATATCGCCCGCGATA
TTCATGAGATTTGTCCCGAAGCATGGCTGATTAACTTTACCAATCCGGCGGGCATCGTGACGGAAGCTTTGCTGAAGCAC
GGACCTCATCAAAAGGTCGTCGGGGTATGCAATATTCCTTACAACATGAAAAATAGCATCGCAGAAATTTTCGGCGTGGA
TGTAAACCTCGTCATGATTGAATTCGCGGGCATCAATCATTTTGTTTTCGGAAAAAGAGTTTGGATCGACGGGATCGACC
GGACAGAAGAGACGATTGAGCTTCTGTTGAATGATCACTTCGGCTACTCGCCTTCCAATATTGCGGCGCTGCCGTGGAAT
CGCCAATTTTTGCGGTCTTTGCGAATGCTTCCGAGCCCTTATCATCAGTACTATTATCAACATGAAGATGCGCTCGCAAA
AGATATCGAAGCGTACAATAACAAAGGGACGCGCGCAGAAACCGTCATGGAAGTGGAGCGGCAGCTGTTTGAGAGATACA
AAGACGCCAAGATCCATGAAAAGCCTAAAGAACTGGAAAGCCGCGGCGGAGCTTACTATAGTGAATCAGCCTGCTCATTA
ATGAACTCGCTTTATCATCATTCAATGGATATTCAGACCGTGAACACACTGAATAAAGGAAGCATACCAGACCTTCCAGA
TGATGCGGTGATCGAAGTCAACAGTGTCATTACAAAAAGCGGACCGATTCCCCTGACCGTCGGAAAACTGCCCGATGCCA
TCAGCGGGCCGATCATCTTGATGAAAAAATTTGAACAATTAGTGATTGAAGCTGCGGTCACCGGCGACTACGATACGGCA
TACTTAGCCATGATCACCAATCCATTGGTCTCTTCTGATCACAAAGCTCAAAAGGTGCTCGATGAAATGCTCGAGGCTCA
TAAACGTTATCTCCCGCAATTTTTTGAGGAGGTCCGTGCACATGAAACAAAATAA

Upstream 100 bases:

>100_bases
ACAATTTGTTATAACAAATATGTATGGACAAATTTGTTATAACATGTTAAGTTTCAATTGTGTGAAGCGCTTTCATAAAA
GAGAGTTAAGGAGGGGGATT

Downstream 100 bases:

>100_bases
AATGATGGCATTCATCGAATCAAACATCATGCCGATCGCCGGAAGGATCGGTTCCCAAAGGCACCTGATTGCAATACGCG
ACGGATTCATCGCCATTATG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MALKVVVIGGGSSYTPEIIEGIIKRHHQFPVNEIVLVDIEEGKEKLRIVSELAKRMIRHAGVPIELSHTIDRAKALKGAD
FVTVQIRVGGLKARALDEKIPLSHGLIGQETNGAGGMFKALRTIPFMLDIARDIHEICPEAWLINFTNPAGIVTEALLKH
GPHQKVVGVCNIPYNMKNSIAEIFGVDVNLVMIEFAGINHFVFGKRVWIDGIDRTEETIELLLNDHFGYSPSNIAALPWN
RQFLRSLRMLPSPYHQYYYQHEDALAKDIEAYNNKGTRAETVMEVERQLFERYKDAKIHEKPKELESRGGAYYSESACSL
MNSLYHHSMDIQTVNTLNKGSIPDLPDDAVIEVNSVITKSGPIPLTVGKLPDAISGPIILMKKFEQLVIEAAVTGDYDTA
YLAMITNPLVSSDHKAQKVLDEMLEAHKRYLPQFFEEVRAHETK

Sequences:

>Translated_444_residues
MALKVVVIGGGSSYTPEIIEGIIKRHHQFPVNEIVLVDIEEGKEKLRIVSELAKRMIRHAGVPIELSHTIDRAKALKGAD
FVTVQIRVGGLKARALDEKIPLSHGLIGQETNGAGGMFKALRTIPFMLDIARDIHEICPEAWLINFTNPAGIVTEALLKH
GPHQKVVGVCNIPYNMKNSIAEIFGVDVNLVMIEFAGINHFVFGKRVWIDGIDRTEETIELLLNDHFGYSPSNIAALPWN
RQFLRSLRMLPSPYHQYYYQHEDALAKDIEAYNNKGTRAETVMEVERQLFERYKDAKIHEKPKELESRGGAYYSESACSL
MNSLYHHSMDIQTVNTLNKGSIPDLPDDAVIEVNSVITKSGPIPLTVGKLPDAISGPIILMKKFEQLVIEAAVTGDYDTA
YLAMITNPLVSSDHKAQKVLDEMLEAHKRYLPQFFEEVRAHETK
>Mature_443_residues
ALKVVVIGGGSSYTPEIIEGIIKRHHQFPVNEIVLVDIEEGKEKLRIVSELAKRMIRHAGVPIELSHTIDRAKALKGADF
VTVQIRVGGLKARALDEKIPLSHGLIGQETNGAGGMFKALRTIPFMLDIARDIHEICPEAWLINFTNPAGIVTEALLKHG
PHQKVVGVCNIPYNMKNSIAEIFGVDVNLVMIEFAGINHFVFGKRVWIDGIDRTEETIELLLNDHFGYSPSNIAALPWNR
QFLRSLRMLPSPYHQYYYQHEDALAKDIEAYNNKGTRAETVMEVERQLFERYKDAKIHEKPKELESRGGAYYSESACSLM
NSLYHHSMDIQTVNTLNKGSIPDLPDDAVIEVNSVITKSGPIPLTVGKLPDAISGPIILMKKFEQLVIEAAVTGDYDTAY
LAMITNPLVSSDHKAQKVLDEMLEAHKRYLPQFFEEVRAHETK

Specific function: Hydrolyzes phospho-beta-glucosides (Probable) [H]

COG id: COG1486

COG function: function code G; Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 4 family [H]

Homologues:

Organism=Escherichia coli, GI1788029, Length=435, Percent_Identity=47.1264367816092, Blast_Score=409, Evalue=1e-115,
Organism=Escherichia coli, GI1790560, Length=454, Percent_Identity=25.7709251101322, Blast_Score=131, Evalue=9e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019802
- InterPro:   IPR001088
- InterPro:   IPR022616
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02056 Glyco_hydro_4; PF11975 Glyco_hydro_4C [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 49769; Mature: 49638

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS01324 GLYCOSYL_HYDROL_F4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALKVVVIGGGSSYTPEIIEGIIKRHHQFPVNEIVLVDIEEGKEKLRIVSELAKRMIRHA
CEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHC
GVPIELSHTIDRAKALKGADFVTVQIRVGGLKARALDEKIPLSHGLIGQETNGAGGMFKA
CCCEEEHHHHHHHHHHCCCCEEEEEEEECCEEHHHHCCCCCCCCCCCCCCCCCCCHHHHH
LRTIPFMLDIARDIHEICPEAWLINFTNPAGIVTEALLKHGPHQKVVGVCNIPYNMKNSI
HHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCCCEEEEECCCCCHHHHH
AEIFGVDVNLVMIEFAGINHFVFGKRVWIDGIDRTEETIELLLNDHFGYSPSNIAALPWN
HHHHCCCEEEEEEEEECCCEEEECCEEEECCCCCHHHHHHHHHHCCCCCCCCCEEECCCC
RQFLRSLRMLPSPYHQYYYQHEDALAKDIEAYNNKGTRAETVMEVERQLFERYKDAKIHE
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCC
KPKELESRGGAYYSESACSLMNSLYHHSMDIQTVNTLNKGSIPDLPDDAVIEVNSVITKS
CHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCEEEEHHHHCCC
GPIPLTVGKLPDAISGPIILMKKFEQLVIEAAVTGDYDTAYLAMITNPLVSSDHKAQKVL
CCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEHHHHHCCCCCCCHHHHHHH
DEMLEAHKRYLPQFFEEVRAHETK
HHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ALKVVVIGGGSSYTPEIIEGIIKRHHQFPVNEIVLVDIEEGKEKLRIVSELAKRMIRHA
EEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHC
GVPIELSHTIDRAKALKGADFVTVQIRVGGLKARALDEKIPLSHGLIGQETNGAGGMFKA
CCCEEEHHHHHHHHHHCCCCEEEEEEEECCEEHHHHCCCCCCCCCCCCCCCCCCCHHHHH
LRTIPFMLDIARDIHEICPEAWLINFTNPAGIVTEALLKHGPHQKVVGVCNIPYNMKNSI
HHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCCCEEEEECCCCCHHHHH
AEIFGVDVNLVMIEFAGINHFVFGKRVWIDGIDRTEETIELLLNDHFGYSPSNIAALPWN
HHHHCCCEEEEEEEEECCCEEEECCEEEECCCCCHHHHHHHHHHCCCCCCCCCEEECCCC
RQFLRSLRMLPSPYHQYYYQHEDALAKDIEAYNNKGTRAETVMEVERQLFERYKDAKIHE
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCC
KPKELESRGGAYYSESACSLMNSLYHHSMDIQTVNTLNKGSIPDLPDDAVIEVNSVITKS
CHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCEEEEHHHHCCC
GPIPLTVGKLPDAISGPIILMKKFEQLVIEAAVTGDYDTAYLAMITNPLVSSDHKAQKVL
CCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEHHHHHCCCCCCCHHHHHHH
DEMLEAHKRYLPQFFEEVRAHETK
HHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8990303; 9384377; 8969509 [H]