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The map label for this gene is lysS [H]

Identifier: 52783938

GI number: 52783938

Start: 93349

End: 94848

Strand: Direct

Name: lysS [H]

Synonym: BLi00097

Alternate gene names: 52783938

Gene position: 93349-94848 (Clockwise)

Preceding gene: 52783937

Following gene: 52783939

Centisome position: 2.21

GC content: 43.67

Gene sequence:

>1500_bases
ATGAGCCATGAAGAGCATAACCATGAAGAATTAAACGACCAGCTGCAAGTCAGACGCGATAAAATGAATCAGTTGAGAGA
AAGCGGAAAAGATCCTTTCGGACAGCGTTTTGATCGCACCCATCAATCAACTGATCTCATTGAGTCGTATAATGAATTCT
CAAAAGAAGAATTAGAGGAAAAAGCAATTGAAGTAACGATTGCCGGCCGGATGATGACAAAGCGCGGAAAAGGGAAAGCG
GGATTCGCGCATATTCAGGATTTAAAGGGCCAGATTCAGATCTATGTCAGAAAAGACAGTGTCGGTGAAGAGCAGTACGA
ATGGTTTAAAAGCTCTGACCTCGGGGACATCGTCGGCGTTACAGGCGTCATGTTTAAAACGAATGTAGGCGAATTGTCTG
TAAAAGCAACATCTTTTGATTTATTGACAAAAGCTCTTCGTCCGCTTCCGGACAAATATCATGGATTAAAGGACATCGAG
CAGCGCTACCGTCAGCGTTATTTGGATTTGATCGTAAATCCGGAAAGCAAAAATACGTTTATTACACGAAGCAAAATCAT
TCAATCCATGAGAAGATACTTAGATGACCACGGATACTTAGAAGTTGAAACGCCTACGATGCACGCGATTCCGGGTGGCG
CATCTGCCCGTCCGTTCATTACACATCATAATGCGCTGGATATGCCGCTTTACATGAGGATCGCTATCGAGCTCCATTTG
AAAAGGCTGATCGTCGGCGGGTTGGAAAAAGTCTATGAAATCGGACGTGTATTCCGAAATGAAGGGGTCTCCACACGGCA
CAATCCTGAATTTACGATGATCGAACTTTACGAAGCTTATGCTGACTATAAGGATATTATGAGATTGACGGAAAATCTCA
TTGCTCATATTGCACAAGAAGTGCTCGGCACTACGACCGTTCAATATGGAGATTATGAAGTCGACCTCAAGCCTGAATGG
AAGAGACTCCATATGGTCGATGCTGTTAAAGAAGCCACCGGTGTAGACTTCTGGAAAGAAGTATCGGTAGAAGAAGCAAG
AGCATATGCCAAAGAACACGGAATTGAAATTTCTGAAGGCATGTCAGTTGGACATATCATTAATGAATTCTTCGAGCAAA
AAGTTGAAGAAACGTTGATTCAGCCTACTTTTATATACGGACACCCTGTGGAGATTTCTCCTCTGGCTAAGAAAAATCCT
GAAGACCCTCGATTTACGGACCGTTTTGAGCTGTTTATCGTGGGCCGTGAGCACGCGAATGCATTTACTGAATTAAATGA
TCCTATCGACCAAAAAGAACGCTTTGAAGCGCAATTAAAAGAACGTGAAGAAGGAAACGATGAAGCGCATATGATGGATG
AAGACTTTGTCGAAGCGCTGGAGTACGGAATGCCTCCTACCGGCGGCCTTGGAATCGGCATCGACAGACTGATTATGCTT
TTGACGAATTCTCCTTCAATCAGGGACGTTTTACTATTCCCGCAAATGAGACATCGTTAG

Upstream 100 bases:

>100_bases
TGCAAAAGTAGGATAAATCCGACCTCTGCTCACTGCCAGTTTTCACTGGCAGTTTTTCTGCTTTTTTCCTGAAAGACTAA
AAGTAACGGAGTGATATATG

Downstream 100 bases:

>100_bases
TTGACTTTCGTGCAGCAGCCGCGTAACAAGCGGCTGCTGCTCATTTAAAACAAATTAAATTTTTTCTCAAAAAAGTATTG
CACAATCATAAATACGGTGG

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 499; Mature: 498

Protein sequence:

>499_residues
MSHEEHNHEELNDQLQVRRDKMNQLRESGKDPFGQRFDRTHQSTDLIESYNEFSKEELEEKAIEVTIAGRMMTKRGKGKA
GFAHIQDLKGQIQIYVRKDSVGEEQYEWFKSSDLGDIVGVTGVMFKTNVGELSVKATSFDLLTKALRPLPDKYHGLKDIE
QRYRQRYLDLIVNPESKNTFITRSKIIQSMRRYLDDHGYLEVETPTMHAIPGGASARPFITHHNALDMPLYMRIAIELHL
KRLIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAYADYKDIMRLTENLIAHIAQEVLGTTTVQYGDYEVDLKPEW
KRLHMVDAVKEATGVDFWKEVSVEEARAYAKEHGIEISEGMSVGHIINEFFEQKVEETLIQPTFIYGHPVEISPLAKKNP
EDPRFTDRFELFIVGREHANAFTELNDPIDQKERFEAQLKEREEGNDEAHMMDEDFVEALEYGMPPTGGLGIGIDRLIML
LTNSPSIRDVLLFPQMRHR

Sequences:

>Translated_499_residues
MSHEEHNHEELNDQLQVRRDKMNQLRESGKDPFGQRFDRTHQSTDLIESYNEFSKEELEEKAIEVTIAGRMMTKRGKGKA
GFAHIQDLKGQIQIYVRKDSVGEEQYEWFKSSDLGDIVGVTGVMFKTNVGELSVKATSFDLLTKALRPLPDKYHGLKDIE
QRYRQRYLDLIVNPESKNTFITRSKIIQSMRRYLDDHGYLEVETPTMHAIPGGASARPFITHHNALDMPLYMRIAIELHL
KRLIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAYADYKDIMRLTENLIAHIAQEVLGTTTVQYGDYEVDLKPEW
KRLHMVDAVKEATGVDFWKEVSVEEARAYAKEHGIEISEGMSVGHIINEFFEQKVEETLIQPTFIYGHPVEISPLAKKNP
EDPRFTDRFELFIVGREHANAFTELNDPIDQKERFEAQLKEREEGNDEAHMMDEDFVEALEYGMPPTGGLGIGIDRLIML
LTNSPSIRDVLLFPQMRHR
>Mature_498_residues
SHEEHNHEELNDQLQVRRDKMNQLRESGKDPFGQRFDRTHQSTDLIESYNEFSKEELEEKAIEVTIAGRMMTKRGKGKAG
FAHIQDLKGQIQIYVRKDSVGEEQYEWFKSSDLGDIVGVTGVMFKTNVGELSVKATSFDLLTKALRPLPDKYHGLKDIEQ
RYRQRYLDLIVNPESKNTFITRSKIIQSMRRYLDDHGYLEVETPTMHAIPGGASARPFITHHNALDMPLYMRIAIELHLK
RLIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAYADYKDIMRLTENLIAHIAQEVLGTTTVQYGDYEVDLKPEWK
RLHMVDAVKEATGVDFWKEVSVEEARAYAKEHGIEISEGMSVGHIINEFFEQKVEETLIQPTFIYGHPVEISPLAKKNPE
DPRFTDRFELFIVGREHANAFTELNDPIDQKERFEAQLKEREEGNDEAHMMDEDFVEALEYGMPPTGGLGIGIDRLIMLL
TNSPSIRDVLLFPQMRHR

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=504, Percent_Identity=42.6587301587302, Blast_Score=394, Evalue=1e-109,
Organism=Homo sapiens, GI194272210, Length=504, Percent_Identity=42.6587301587302, Blast_Score=393, Evalue=1e-109,
Organism=Homo sapiens, GI40789249, Length=246, Percent_Identity=26.8292682926829, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI4758762, Length=326, Percent_Identity=24.2331288343558, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1790571, Length=490, Percent_Identity=55.1020408163265, Blast_Score=544, Evalue=1e-156,
Organism=Escherichia coli, GI1789256, Length=490, Percent_Identity=54.6938775510204, Blast_Score=538, Evalue=1e-154,
Organism=Escherichia coli, GI87082379, Length=337, Percent_Identity=30.8605341246291, Blast_Score=151, Evalue=9e-38,
Organism=Escherichia coli, GI1788173, Length=277, Percent_Identity=28.158844765343, Blast_Score=96, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17535925, Length=489, Percent_Identity=46.6257668711656, Blast_Score=431, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17535927, Length=489, Percent_Identity=46.6257668711656, Blast_Score=431, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI71994340, Length=489, Percent_Identity=46.6257668711656, Blast_Score=430, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17551876, Length=467, Percent_Identity=22.2698072805139, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI32566633, Length=266, Percent_Identity=25.187969924812, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320242, Length=512, Percent_Identity=42.3828125, Blast_Score=398, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6324256, Length=554, Percent_Identity=31.5884476534296, Blast_Score=211, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6323011, Length=465, Percent_Identity=23.8709677419355, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6321807, Length=323, Percent_Identity=27.8637770897833, Blast_Score=93, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24640849, Length=505, Percent_Identity=41.980198019802, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24640851, Length=505, Percent_Identity=41.980198019802, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI17136276, Length=465, Percent_Identity=23.010752688172, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24584738, Length=266, Percent_Identity=27.4436090225564, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 57691; Mature: 57560

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHEEHNHEELNDQLQVRRDKMNQLRESGKDPFGQRFDRTHQSTDLIESYNEFSKEELEE
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KAIEVTIAGRMMTKRGKGKAGFAHIQDLKGQIQIYVRKDSVGEEQYEWFKSSDLGDIVGV
HHEEEEEECHHHHCCCCCCCCHHHHHCCCCEEEEEEECCCCCHHHHHHHHCCCCHHHHHH
TGVMFKTNVGELSVKATSFDLLTKALRPLPDKYHGLKDIEQRYRQRYLDLIVNPESKNTF
HHHEEECCCCCEEEEHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHEECCCCCCCC
ITRSKIIQSMRRYLDDHGYLEVETPTMHAIPGGASARPFITHHNALDMPLYMRIAIELHL
HHHHHHHHHHHHHHCCCCEEEEECCEEEECCCCCCCCCEEEECCCCCCCHHHHHHHHHHH
KRLIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAYADYKDIMRLTENLIAHIAQE
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLGTTTVQYGDYEVDLKPEWKRLHMVDAVKEATGVDFWKEVSVEEARAYAKEHGIEISEG
HHCCCEEEECCEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHCC
MSVGHIINEFFEQKVEETLIQPTFIYGHPVEISPLAKKNPEDPRFTDRFELFIVGREHAN
CHHHHHHHHHHHHHHHHHHCCCEEEECCCCEECCCCCCCCCCCCCCCEEEEEEEECHHHH
AFTELNDPIDQKERFEAQLKEREEGNDEAHMMDEDFVEALEYGMPPTGGLGIGIDRLIML
HHHHCCCCCCHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHCCCCCCCCCCCHHHHHHH
LTNSPSIRDVLLFPQMRHR
HCCCCCHHHEEECCHHCCC
>Mature Secondary Structure 
SHEEHNHEELNDQLQVRRDKMNQLRESGKDPFGQRFDRTHQSTDLIESYNEFSKEELEE
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KAIEVTIAGRMMTKRGKGKAGFAHIQDLKGQIQIYVRKDSVGEEQYEWFKSSDLGDIVGV
HHEEEEEECHHHHCCCCCCCCHHHHHCCCCEEEEEEECCCCCHHHHHHHHCCCCHHHHHH
TGVMFKTNVGELSVKATSFDLLTKALRPLPDKYHGLKDIEQRYRQRYLDLIVNPESKNTF
HHHEEECCCCCEEEEHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHEECCCCCCCC
ITRSKIIQSMRRYLDDHGYLEVETPTMHAIPGGASARPFITHHNALDMPLYMRIAIELHL
HHHHHHHHHHHHHHCCCCEEEEECCEEEECCCCCCCCCEEEECCCCCCCHHHHHHHHHHH
KRLIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAYADYKDIMRLTENLIAHIAQE
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLGTTTVQYGDYEVDLKPEWKRLHMVDAVKEATGVDFWKEVSVEEARAYAKEHGIEISEG
HHCCCEEEECCEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHCC
MSVGHIINEFFEQKVEETLIQPTFIYGHPVEISPLAKKNPEDPRFTDRFELFIVGREHAN
CHHHHHHHHHHHHHHHHHHCCCEEEECCCCEECCCCCCCCCCCCCCCEEEEEEEECHHHH
AFTELNDPIDQKERFEAQLKEREEGNDEAHMMDEDFVEALEYGMPPTGGLGIGIDRLIML
HHHHCCCCCCHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHCCCCCCCCCCCHHHHHHH
LTNSPSIRDVLLFPQMRHR
HCCCCCHHHEEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]